Volume 18 Issue 11, November 2017

Volume 18 Issue 11

'Editing the mRNA film' by Vicky Summersby, inspired by the Review on p655.

Research Highlights

  • Research Highlight |

    The size and activity of nucleoli are cellular hallmarks of longevity and ageing, in model organisms and in humans.

    • Eytan Zlotorynski
  • Research Highlight |

    Low-level transcription persists during mitosis, and the reinstatement of robust gene expression occurs in a stepwise manner, starting with genes regulating cell organization and growth followed by the expression of cell type-specific genes.

    • Paulina Strzyz
  • Journal Club

Reviews

  • Review Article |

    Atomic-resolution structures have recently been obtained for the intact spliceosome at different stages of the splicing cycle. These structural data have proved that the spliceosome is a protein-directed metalloribozyme and have increased our understanding of pre-mRNA splicing mechanisms, explaining a large body of existing genetic and biochemical data.

    • Yigong Shi
  • Review Article |

    Lipolysis degrades triacylglycerols to supply cells with free fatty acids, which are essential components of membrane lipids and substrates for energy production. Recent discoveries transformed our understanding of the functions of and crosstalk between 'neutral' lipolysis, which occurs in the cytosol, and lipophagy and 'acid' lipolysis, which occur in lysosomes, and how dysfunction in these processes contributes to metabolic diseases.

    • Rudolf Zechner
    • , Frank Madeo
    •  & Dagmar Kratky
  • Review Article |

    Fluorescence nanoscopy enables the optical imaging of cellular components with resolutions at the nanometre scale. With the growing availability of super-resolution microscopes, nanoscopy methods are being increasingly applied. Quantitative, multicolour, live-cell nanoscopy and the corresponding labelling strategies are under continuous development.

    • Steffen J. Sahl
    • , Stefan W. Hell
    •  & Stefan Jakobs
  • Review Article |

    Assembly of the microtubule nucleus is energetically unfavourable and, in vivo, microtubule nucleation requires support, such as a stable template, to stabilize the initial weak interactions between tubulin dimers. Microtubules can also be nucleated in the absence of a template by certain microtubule-associated proteins, which stabilize the nascent nucleation intermediates.

    • Johanna Roostalu
    •  & Thomas Surrey