Volume 15 Issue 11, November 2020

This protocol describes the light-induced functionalization of proteins with compounds bearing the photochemically active aryl azide group. The procedure is exemplified by the light-induced radiosynthesis of zirconium-89 radiolabeled mAbs.

The authors describe an approach for optimizing transcranial magnetic stimulation (TMS) targeting by combining functional magnetic resonance imaging and iterative electric-field stimulation.

This protocol describes how to record intracellular neuronal activity in awake nonhuman primates in response to external stimuli. With the help of a coaxial guide tube, multiple neurons can be recorded over longer times from a single individual.

Here, the authors describe step-by-step procedures for integrating single-cell sequencing datasets from different experiments or modalities to identify common and distinct cell types using the R-based software tool LIGER.

The authors describe how to easily prepare a large number of dipsticks from cellulose-based filter paper and use them to rapidly purify nucleic acids from a variety of sources.

This protocol assesses proinflammatory properties of nucleic acid nanoparticles (NANPs) in PBMCs, which are highly predictive of cytokine responses. The authors detail how to prepare NANPs and analyze characteristic biomarkers produced by PBMCs transfected with NANPs.

The production and titration of the SARS-CoV-2 S pseudotyped virus using a VSV-based pseudovirus production system in this protocol enable its use under biosafety level 2 conditions as well as in a neutralization assay to assess the level of neutralizing antibodies or molecular inhibitors in a sample.

This protocol describes how to generate oligodendrocyte precursor cells from human pluripotent stem cells that can subsequently myelinate neurons, both in vitro and in vivo.

RepeatExplorer is a software tool for repeat identification and quantification using unassembled sequencing reads. The authors describe four pipelines implemented on the Galaxy platform, highlighting different applications.