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In this tutorial, the authors provide a comprehensive description of the considerations for designing single-cell transcriptomics studies, from sample preparation and single-cell RNA sequencing methodologies through data processing and analysis.
This Perspective discusses the development of the linear amplified RNA amplification technique over the last 25 years, and future applications of this important and versatile methodology.
Here Sarah Teichmann and colleagues provide a Perspective on the exponential scaling of single-cell RNA-sequencing experiments over the last decade, commenting on the methodological developments that have underpinned the advances in this technology.