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This protocol describes an approach to quantifying DNA replication dynamics (initiation and termination frequencies and origin firing efficiencies) at defined genomic loci in asynchronously growing cells.
A new protocol for solid-state NMR of soluble and membrane proteins in E. coli, in both whole cells and isolated membrane fractions. The procedure describes conventional 13C/15N ssNMR as well as sensitivity-enhanced DNP-ssNMR and 1H-detected ssNMR.
This protocol describes VAMPIRE, an unsupervised machine-learning approach that can be used to quantify and categorize cellular morphology from fluorescence or bright-field images of cells grown in 2D, 3D and tissue slices.
Mancuso and De Strooper and colleagues describe MIGRATE (microglia in vitro generation refined for advanced transplantation experiments, a combined in vitro differentiation and in vivo xenotransplantation protocol for studying human microglia transplanted into mouse brain.
Optimization of chemical reactions can be facilitated by techniques that enable experiments to be set up in parallel. In this work, 96 Pd–catalyzed cross-coupling experiments are performed and analyzed in parallel with commonly available equipment.
The authors discuss experimental design considerations and describe a computational pipeline to reveal the synergistic and additive effects of combinatorial perturbations on gene expression measured by RNA sequencing.
Takebe et al. describe a protocol for the continuous patterning of hepatic, biliary and pancreatic structures from a 3D culture of human pluripotent stem cells.