Articles

  • Article | | open

    The recovery of 7,903 bacterial and archaeal metagenome-assembled genomes increases the phylogenetic diversity represented by public genome repositories and provides the first representatives from 20 candidate phyla.

    • Donovan H. Parks
    • , Christian Rinke
    • , Maria Chuvochina
    • , Pierre-Alain Chaumeil
    • , Ben J. Woodcroft
    • , Paul N. Evans
    • , Philip Hugenholtz
    •  & Gene W. Tyson
  • Article |

    The structures of a variant surface glycoprotein (VSG) from Trypanosoma brucei suggest that VSGs adopt different conformations to respond to obstacles present in the cell membrane, enabling them to maintain a protective coat at all times.

    • Thomas Bartossek
    • , Nicola G. Jones
    • , Christin Schäfer
    • , Mislav Cvitković
    • , Marius Glogger
    • , Helen R. Mott
    • , Jochen Kuper
    • , Martha Brennich
    • , Mark Carrington
    • , Ana-Sunčana Smith
    • , Susanne Fenz
    • , Caroline Kisker
    •  & Markus Engstler
  • Article |

    CD81 is shown to interact with SAMHD1 and lead to its proteasomal degradation, thereby impacting dNTP availability and enhancing HIV-1 reverse transcription in primary human T cells.

    • Vera Rocha-Perugini
    • , Henar Suárez
    • , Susana Álvarez
    • , Soraya López-Martín
    • , Gina M. Lenzi
    • , Felipe Vences-Catalán
    • , Shoshana Levy
    • , Baek Kim
    • , María A. Muñoz-Fernández
    • , Francisco Sánchez-Madrid
    •  & Maria Yáñez-Mó
  • Article |

    TRIM23 is identified as an essential regulator of virus-induced autophagy that mediates restriction to several RNA and DNA viruses. K27-mediated ubiquitylation activates TRIM23 GTPase activity, triggering its relocalization and selective autophagy.

    • Konstantin M. J. Sparrer
    • , Sebastian Gableske
    • , Matthew A. Zurenski
    • , Zachary M. Parker
    • , Florian Full
    • , Gavin J. Baumgart
    • , Jiro Kato
    • , Gustavo Pacheco-Rodriguez
    • , Chengyu Liang
    • , Owen Pornillos
    • , Joel Moss
    • , Martha Vaughan
    •  & Michaela U. Gack
  • Article |

    Chikungunya virus infection leads to painful arthritis-like joint inflammation. This study shows that the NLRP3 inflammasome is crucial for alphavirus-induced inflammation and its inhibition is an effective therapeutic strategy.

    • Weiqiang Chen
    • , Suan-Sin Foo
    • , Ali Zaid
    • , Terk-Shin Teng
    • , Lara J. Herrero
    • , Stefan Wolf
    • , Kothila Tharmarajah
    • , Luan D. Vu
    • , Caryn van Vreden
    • , Adam Taylor
    • , Joseph R. Freitas
    • , Rachel W. Li
    • , Trent M. Woodruff
    • , Richard Gordon
    • , David M. Ojcius
    • , Helder I. Nakaya
    • , Thirumala-Devi Kanneganti
    • , Luke A. J. O’Neill
    • , Avril A. B. Robertson
    • , Nicholas J. King
    • , Andreas Suhrbier
    • , Matthew A. Cooper
    • , Lisa F. P. Ng
    •  & Suresh Mahalingam
  • Article |

    This study reports the identification of broadly protective antibodies targeting the influenza B neuraminidase away from its active site. One dose of antibody therapy was more protective in mice than multiple doses of the current standard of care.

    • Teddy John Wohlbold
    • , Kira A. Podolsky
    • , Veronika Chromikova
    • , Ericka Kirkpatrick
    • , Veronica Falconieri
    • , Philip Meade
    • , Fatima Amanat
    • , Jessica Tan
    • , Benjamin R. tenOever
    • , Gene S. Tan
    • , Sriram Subramaniam
    • , Peter Palese
    •  & Florian Krammer
  • Article |

    Both African and epidemic strains of Zika virus are shown to target CD14+ monocytes, which are more susceptible in pregnant women, but African strains are associated with inflammatory responses, and epidemic strains with immunotolerance.

    • Suan-Sin Foo
    • , Weiqiang Chen
    • , Yen Chan
    • , James W. Bowman
    • , Lin-Chun Chang
    • , Younho Choi
    • , Ji Seung Yoo
    • , Jianning Ge
    • , Genhong Cheng
    • , Alexandre Bonnin
    • , Karin Nielsen-Saines
    • , Patrícia Brasil
    •  & Jae U. Jung
  • Article |

    A new class of drugs, hexahydroquinolines, inhibits host haemoglobin endocytosis by Plasmodium falciparum and displays both therapeutic and transmission-blocking activities.

    • Manu Vanaerschot
    • , Leonardo Lucantoni
    • , Tao Li
    • , Jill M. Combrinck
    • , Andrea Ruecker
    • , T. R. Santha Kumar
    • , Kelly Rubiano
    • , Pedro E. Ferreira
    • , Giulia Siciliano
    • , Sonia Gulati
    • , Philipp P. Henrich
    • , Caroline L. Ng
    • , James M. Murithi
    • , Victoria C. Corey
    • , Sandra Duffy
    • , Ori J. Lieberman
    • , M. Isabel Veiga
    • , Robert E. Sinden
    • , Pietro Alano
    • , Michael J. Delves
    • , Kim Lee Sim
    • , Elizabeth A. Winzeler
    • , Timothy J. Egan
    • , Stephen L. Hoffman
    • , Vicky M. Avery
    •  & David A. Fidock
  • Article |

    During acute HIV type 1 infection, a subset of γδ T cells that express Δ42PD1 are shown to home to the gut, where they activate innate immunity and inflammation through direct interaction of Δ42PD1 with Toll-like receptor 4. Blockade of this pathway reduces mucosal damage.

    • Allen Ka Loon Cheung
    • , Hau-yee Kwok
    • , Yiru Huang
    • , Min Chen
    • , Yufei Mo
    • , Xilin Wu
    • , Ka-shing Lam
    • , Hoi-Kuan Kong
    • , Terrence Chi Kong Lau
    • , Jingying Zhou
    • , Jingjing Li
    • , Lin Cheng
    • , Boon Kiat Lee
    • , Qiaoli Peng
    • , Xiaofan Lu
    • , Minghui An
    • , Hui Wang
    • , Hong Shang
    • , Boping Zhou
    • , Hao Wu
    • , Aimin Xu
    • , Kwok-Yung Yuen
    •  & Zhiwei Chen
  • Article |

    A genome-wide association approach identifies differential biofilm and virulence attributes associated with mortality in two Staphylococcus aureus clonal complexes.

    • Mario Recker
    • , Maisem Laabei
    • , Michelle S. Toleman
    • , Sandra Reuter
    • , Rebecca B. Saunderson
    • , Beth Blane
    • , M. Estee Török
    • , Khadija Ouadi
    • , Emily Stevens
    • , Maho Yokoyama
    • , Joseph Steventon
    • , Luke Thompson
    • , Gregory Milne
    • , Sion Bayliss
    • , Leann Bacon
    • , Sharon J. Peacock
    •  & Ruth C. Massey
  • Article |

    The group A streptococcal M protein virulence factor induces inflammasome activation during infection.

    • J. Andrés Valderrama
    • , Angelica M. Riestra
    • , Nina J. Gao
    • , Christopher N. LaRock
    • , Naveen Gupta
    • , Syed Raza Ali
    • , Hal M. Hoffman
    • , Partho Ghosh
    •  & Victor Nizet
  • Article |

    In situ analysis of natural Crocosphaera populations revealed diel cycling of transcript abundances for a range of metabolic pathways, indicating that these cyanobacteria are dominant nitrogen fixers and contributors to primary productivity.

    • Samuel T. Wilson
    • , Frank O. Aylward
    • , Francois Ribalet
    • , Benedetto Barone
    • , John R. Casey
    • , Paige E. Connell
    • , John M. Eppley
    • , Sara Ferrón
    • , Jessica N. Fitzsimmons
    • , Christopher T. Hayes
    • , Anna E. Romano
    • , Kendra A. Turk-Kubo
    • , Alice Vislova
    • , E. Virginia Armbrust
    • , David A. Caron
    • , Matthew J. Church
    • , Jonathan P. Zehr
    • , David M. Karl
    •  & Edward F. DeLong
  • Article |

    A structural analysis of the C-terminal extension of DotL and its binding partners DotN, IcmS, IcmW and LvgA elucidates how the type IV secretion system (T4SS) of Legionella pneumophila mediates the recruitment of T4SS effectors.

    • Mi-Jeong Kwak
    • , J. Dongun Kim
    • , Hyunmin Kim
    • , Cheolhee Kim
    • , James W. Bowman
    • , Seonghoon Kim
    • , Keehyoung Joo
    • , Jooyoung Lee
    • , Kyeong Sik Jin
    • , Yeon-Gil Kim
    • , Nam Ki Lee
    • , Jae U. Jung
    •  & Byung-Ha Oh
  • Article |

    A lack of models has hindered the study of Ebola virus (EBOV) persistence and sequelae, which are now shown to occur in rhesus monkeys. Progressive EBOV spread into eyes, brain and testes with CD68+ cells as the cryptic EBOV reservoir.

    • Xiankun Zeng
    • , Candace D. Blancett
    • , Keith A. Koistinen
    • , Christopher W. Schellhase
    • , Jeremy J. Bearss
    • , Sheli R. Radoshitzky
    • , Shelley P. Honnold
    • , Taylor B. Chance
    • , Travis K. Warren
    • , Jeffrey W. Froude
    • , Kathleen A. Cashman
    • , John M. Dye
    • , Sina Bavari
    • , Gustavo Palacios
    • , Jens H. Kuhn
    •  & Mei G. Sun
  • Article |

    Development of a broad-spectrum β-lactamase inhibitor capable of combatting multidrug-resistant pathogens, including Acinetobacter baumannii.

    • Thomas F. Durand-Réville
    • , Satenig Guler
    • , Janelle Comita-Prevoir
    • , Brendan Chen
    • , Neil Bifulco
    • , Hoan Huynh
    • , Sushmita Lahiri
    • , Adam B. Shapiro
    • , Sarah M. McLeod
    • , Nicole M. Carter
    • , Samir H. Moussa
    • , Camilo Velez-Vega
    • , Nelson B. Olivier
    • , Robert McLaughlin
    • , Ning Gao
    • , Jason Thresher
    • , Tiffany Palmer
    • , Beth Andrews
    • , Robert A. Giacobbe
    • , Joseph V. Newman
    • , David E. Ehmann
    • , Boudewijn de Jonge
    • , John O'Donnell
    • , John P. Mueller
    • , Rubén A. Tommasi
    •  & Alita A. Miller
  • Article |

    Structural analysis of the type VI secretion TssK baseplate protein shows structural similarities with phage receptor-binding protein.

    • Van Son Nguyen
    • , Laureen Logger
    • , Silvia Spinelli
    • , Pierre Legrand
    • , Thi Thanh Huyen Pham
    • , Thi Trang Nhung Trinh
    • , Yassine Cherrak
    • , Abdelrahim Zoued
    • , Aline Desmyter
    • , Eric Durand
    • , Alain Roussel
    • , Christine Kellenberger
    • , Eric Cascales
    •  & Christian Cambillau
  • Article |

    Cycloclasticus bacterial symbionts of mussels and sponges that live in deep-sea gas and oil seeps are capable of using short-chain alkanes as their primary energy source, providing further insight into chemosynthetic symbioses.

    • Maxim Rubin-Blum
    • , Chakkiath Paul Antony
    • , Christian Borowski
    • , Lizbeth Sayavedra
    • , Thomas Pape
    • , Heiko Sahling
    • , Gerhard Bohrmann
    • , Manuel Kleiner
    • , Molly C. Redmond
    • , David L. Valentine
    •  & Nicole Dubilier
  • Article |

    Sequence-specific interactions between the Hepatitis B virus pre-genome and the core protein define the nucleocapsid assembly pathway and trigger virus-like particle formation. Cp-RNA contacts may regulate pre-genome organization, facilitating reverse transcription.

    • Nikesh Patel
    • , Simon J. White
    • , Rebecca F. Thompson
    • , Richard Bingham
    • , Eva U. Weiß
    • , Daniel P. Maskell
    • , Adam Zlotnick
    • , Eric C. Dykeman
    • , Roman Tuma
    • , Reidun Twarock
    • , Neil A. Ranson
    •  & Peter G. Stockley
  • Article |

    Virus attenuation is used to obtain vaccine strains. Here, the rapid evolution of RNA viruses is exploited by engineering their genomes to encode sites that are a mutation away from a stop codon, a clever method to generate attenuated viruses.

    • Gonzalo Moratorio
    • , Rasmus Henningsson
    • , Cyril Barbezange
    • , Lucia Carrau
    • , Antonio V. Bordería
    • , Hervé Blanc
    • , Stephanie Beaucourt
    • , Enzo Z. Poirier
    • , Thomas Vallet
    • , Jeremy Boussier
    • , Bryan C. Mounce
    • , Magnus Fontes
    •  & Marco Vignuzzi
  • Article |

    Methanonatronarchaeia are a distinct class-level lineage of extremely halophilic methanogens, which lack features of classical methanogenesis and have a high intracellular concentration of potassium, suggesting potassium-based osmoprotection.

    • Dimitry Y. Sorokin
    • , Kira S. Makarova
    • , Ben Abbas
    • , Manuel Ferrer
    • , Peter N. Golyshin
    • , Erwin A. Galinski
    • , Sergio Ciordia
    • , María Carmen Mena
    • , Alexander Y. Merkel
    • , Yuri I. Wolf
    • , Mark C. M. van Loosdrecht
    •  & Eugene V. Koonin