Articles

  • Article |

    The cryo-EM structures of Cas12g in complex with sgRNA in the absence and presence of target RNA reveal that the duplex formed by target RNA and crRNA binds to a central channel of Cas12g, inducing its conformational change and activation.

    • Zhuang Li
    • , Heng Zhang
    • , Renjian Xiao
    • , Ruijie Han
    •  & Leifu Chang
  • Article |

    In honey bee colonies infested with the mite Varroa destructor, six Varroa-parasitization-specific (VPS) compounds trigger protective behavior in the bees, which are able to distinguish VPS compounds from healthy signals and recognize an infested brood.

    • Fanny Mondet
    • , Solene Blanchard
    • , Nicolas Barthes
    • , Dominique Beslay
    • , Celia Bordier
    • , Guy Costagliola
    • , Maxime R. Hervé
    • , Benoit Lapeyre
    • , Seo Hyun Kim
    • , Benjamin Basso
    • , Alison R. Mercer
    •  & Yves Le Conte
  • Article |

    E3 ligase subunit protein Fbxo48 interacts with phosphorylated Ampkα and mediates its proteasomal degradation. Interruption of the pAmpkα/Fbxo48 interaction by a small-molecule BC1618 promoted Ampkα activation and improved insulin sensitivity.

    • Yuan Liu
    • , Michael J. Jurczak
    • , Travis B. Lear
    • , Bo Lin
    • , Mads B. Larsen
    • , Jason R. Kennerdell
    • , Yanwen Chen
    • , Brydie R. Huckestein
    • , Matthew K. Nguyen
    • , Ferhan Tuncer
    • , Yu Jiang
    • , Satdarshan P. Monga
    • , Christopher P. O’Donnell
    • , Toren Finkel
    • , Bill B. Chen
    •  & Rama K. Mallampalli
  • Article |

    Crystal structures of a cobalamin-dependent radical S-adenosylmethionine (SAM) methylase reveal an unexpected mechanism that involves substrate-assisted catalysis whereby the carboxylate group of the co-substrate SAM serves as a general base.

    • Hayley L. Knox
    • , Percival Yang-Ting Chen
    • , Anthony J. Blaszczyk
    • , Arnab Mukherjee
    • , Tyler L. Grove
    • , Erica L. Schwalm
    • , Bo Wang
    • , Catherine L. Drennan
    •  & Squire J. Booker
  • Article |

    Structural analysis of the A2058-dimethylated and unmethylated 70S ribosome complex alone and in combination with macrolides reveals the role of the desosamine moiety of macrolides in drug binding and resistance.

    • Maxim S. Svetlov
    • , Egor A. Syroegin
    • , Elena V. Aleksandrova
    • , Gemma C. Atkinson
    • , Steven T. Gregory
    • , Alexander S. Mankin
    •  & Yury S. Polikanov
  • Article |

    Bacteriophage single-stranded DNA annealing proteins (SSAPs) interact with the C termini of single-stranded binding proteins in host bacteria, a finding that enables engineering of enhanced SSAP portability and DNA recombineering activities.

    • Gabriel T. Filsinger
    • , Timothy M. Wannier
    • , Felix B. Pedersen
    • , Isaac D. Lutz
    • , Julie Zhang
    • , Devon A. Stork
    • , Anik Debnath
    • , Kevin Gozzi
    • , Helene Kuchwara
    • , Verena Volf
    • , Stan Wang
    • , Xavier Rios
    • , Christopher J. Gregg
    • , Marc J. Lajoie
    • , Seth L. Shipman
    • , John Aach
    • , Michael T. Laub
    •  & George M. Church
  • Article |

    A chemical screen identified BET bromodomain inhibitors as promoters of keratinocyte regenerative function and skin wound healing. Specifically, low-dose transient treatment with BET inhibitors imposes an activated, migratory state in keratinocytes.

    • Gabi Schutzius
    • , Christian Kolter
    • , Sebastian Bergling
    • , Federico Tortelli
    • , Florian Fuchs
    • , Steffen Renner
    • , Vito Guagnano
    • , Simona Cotesta
    • , Heinrich Rueeger
    • , Michael Faller
    • , Laure Bouchez
    • , Adrian Salathe
    • , Florian Nigsch
    • , Shola M. Richards
    • , Malvina Louis
    • , Viktoria Gruber
    • , Alexandra Aebi
    • , Jonathan Turner
    • , Frederic Grandjean
    • , Jun Li
    • , Chris Dimitri
    • , Jason R. Thomas
    • , Markus Schirle
    • , Jutta Blank
    • , Peter Drueckes
    • , Andrea Vaupel
    • , Ralph Tiedt
    • , Paul W. Manley
    • , Julia Klopp
    • , Rene Hemmig
    • , Florence Zink
    • , Nelly Leroy
    • , Walter Carbone
    • , Guglielmo Roma
    • , Caroline Gubser Keller
    • , Natalie Dales
    • , Armin Beyerbach
    • , Alfred Zimmerlin
    • , Debora Bonenfant
    • , Remi Terranova
    • , Amy Berwick
    • , Sukhdeep Sahambi
    • , Aimee Reynolds
    • , Lori L. Jennings
    • , Heinz Ruffner
    • , Peter Tarsa
    • , Tewis Bouwmeester
    • , Vickie Driver
    • , Mathias Frederiksen
    • , Felix Lohmann
    •  & Susan Kirkland
  • Article |

    Unlike other amine oxidase-family enzymes, nicotine oxidoreductase (NicA2) reacts very slowly with oxygen, prompting the search for and identification of a cytochrome c protein (CycN) that is responsible for accepting electrons from NicA2 in vivo.

    • Mark Dulchavsky
    • , Christopher T. Clark
    • , James C. A. Bardwell
    •  & Frederick Stull
  • Article |

    Laboratory evolution of the bacterial transpeptidase sortase A coupled with yeast display selection enables a change of the enzyme’s substrate preference to recognize and covalently label endogenous amyloid-β protein, impeding the protein’s ability to aggregate.

    • Christopher J. Podracky
    • , Chihui An
    • , Alexandra DeSousa
    • , Brent M. Dorr
    • , Dominic M. Walsh
    •  & David R. Liu
  • Article |

    A new DNA data storage technology—data recording in vivo by electrical stimulation (DRIVES)—places CRISPR-based DNA encoding activity under electrochemical control by coupling cellular redox state to CRISPR array gene expression.

    • Sung Sun Yim
    • , Ross M. McBee
    • , Alan M. Song
    • , Yiming Huang
    • , Ravi U. Sheth
    •  & Harris H. Wang
  • Article |

    Single-molecule FRET of mGluR2 shows that the conformations of the ligand-binding domain and the linked cysteine-rich domain are loosely coupled during ligand-induced activation and defines two pre-active states linking inactive and active states.

    • Brandon Wey-Hung Liauw
    • , Hamid Samareh Afsari
    •  & Reza Vafabakhsh
  • Article |

    Crystal structures of FEM1C in apo and in complex with a C-degron ending with arginine reveal a binding pocket in FEM1C that recognizes C-degrons and the essential role of C-terminal arginine for recognition.

    • Xiaojie Yan
    • , Xiaolu Wang
    • , Yao Li
    • , Mengqi Zhou
    • , Yanjun Li
    • , Lili Song
    • , Wenyi Mi
    • , Jinrong Min
    •  & Cheng Dong
  • Article |

    Beginning with a functional site and building a supporting scaffold around it enables the de novo design of proteins with distinct binding motifs for use in biosensors to detect antibody responses and as ligands of synthetic signaling receptors.

    • Che Yang
    • , Fabian Sesterhenn
    • , Jaume Bonet
    • , Eva A. van Aalen
    • , Leo Scheller
    • , Luciano A. Abriata
    • , Johannes T. Cramer
    • , Xiaolin Wen
    • , Stéphane Rosset
    • , Sandrine Georgeon
    • , Theodore Jardetzky
    • , Thomas Krey
    • , Martin Fussenegger
    • , Maarten Merkx
    •  & Bruno E. Correia
  • Article |

    Structural characterization of the substrate adapters of the C-degron pathway reveals the selective recognition mode towards substrates with C-terminal arginine by FEM1A/C and FEM1B.

    • Xinyan Chen
    • , Shanhui Liao
    • , Yaara Makaros
    • , Qiong Guo
    • , Zhongliang Zhu
    • , Rina Krizelman
    • , Karin Dahan
    • , Xiaoming Tu
    • , Xuebiao Yao
    • , Itay Koren
    •  & Chao Xu
  • Article |

    Coupling light-inducible bacterial biofilm formation with hydroxyapatite mineralization enables the synthesis of living patterned and gradient composite biomaterials with control over the degree of mineralization and the ability to self-heal.

    • Yanyi Wang
    • , Bolin An
    • , Bin Xue
    • , Jiahua Pu
    • , Xiuli Zhang
    • , Yuanyuan Huang
    • , Yi Yu
    • , Yi Cao
    •  & Chao Zhong
  • Article |

    Using synthetic ubiquitins with non-natural acceptor site, the authors revealed that the length of lysine side chain in acceptor ubiquitins affects ubiquitin chain linkage specificity with native lysine as the preferred geometry.

    • Joanna Liwocha
    • , David T. Krist
    • , Gerbrand J. van der Heden van Noort
    • , Fynn M. Hansen
    • , Vinh H. Truong
    • , Ozge Karayel
    • , Nicholas Purser
    • , Daniel Houston
    • , Nicole Burton
    • , Mark J. Bostock
    • , Michael Sattler
    • , Matthias Mann
    • , Joseph S. Harrison
    • , Gary Kleiger
    • , Huib Ovaa
    •  & Brenda A. Schulman
  • Article |

    Rather than their expected role in self-immunity, kinases encoded in the biosynthetic gene clusters of nucleoside natural products catalyze the phosphorylation of an early intermediate, a modification that is later removed in a downstream step.

    • Matthew M. Draelos
    • , Anyarat Thanapipatsiri
    • , Hilda Sucipto
    •  & Kenichi Yokoyama
  • Article |

    The authors report PROTAC ternary complex structures involving the E3 ligase cIAP1 and target protein BTK, showing that cooperativity is not always correlated with degradation efficiency.

    • James Schiemer
    • , Reto Horst
    • , Yilin Meng
    • , Justin I. Montgomery
    • , Yingrong Xu
    • , Xidong Feng
    • , Kris Borzilleri
    • , Daniel P. Uccello
    • , Carolyn Leverett
    • , Stephen Brown
    • , Ye Che
    • , Matthew F. Brown
    • , Matthew M. Hayward
    • , Adam M. Gilbert
    • , Mark C. Noe
    •  & Matthew F. Calabrese
  • Article |

    A proximity labeling strategy enables enrichment of cell type-selective secretomes in mice by direct biochemical purification of biotinylated polypeptides from blood plasma.

    • Wei Wei
    • , Nicholas M. Riley
    • , Andrew C. Yang
    • , Joon T. Kim
    • , Stephanie M. Terrell
    • , Veronica L. Li
    • , Marta Garcia-Contreras
    • , Carolyn R. Bertozzi
    •  & Jonathan Z. Long
  • Article |

    Qemistree uses fragmentation spectra to predict molecular fingerprints and represent their relationships as a tree, enabling comparison of metabolomics data across different experimental conditions and exploration of chemical diversity in mixtures.

    • Anupriya Tripathi
    • , Yoshiki Vázquez-Baeza
    • , Julia M. Gauglitz
    • , Mingxun Wang
    • , Kai Dührkop
    • , Mélissa Nothias-Esposito
    • , Deepa D. Acharya
    • , Madeleine Ernst
    • , Justin J. J. van der Hooft
    • , Qiyun Zhu
    • , Daniel McDonald
    • , Asker D. Brejnrod
    • , Antonio Gonzalez
    • , Jo Handelsman
    • , Markus Fleischauer
    • , Marcus Ludwig
    • , Sebastian Böcker
    • , Louis-Félix Nothias
    • , Rob Knight
    •  & Pieter C. Dorrestein
  • Article |

    Native mass spectrometry, HDX-MS and MD simulations define the mechanism for how LPS binding to the Gram-negative outer membrane complex LptDE opens the LptD lateral exit gate and how thanatin impairs transport across the periplasm.

    • Francesco Fiorentino
    • , Joshua B. Sauer
    • , Xingyu Qiu
    • , Robin A. Corey
    • , C. Keith Cassidy
    • , Benjamin Mynors-Wallis
    • , Shahid Mehmood
    • , Jani R. Bolla
    • , Phillip J. Stansfeld
    •  & Carol V. Robinson
  • Article |

    The authors identify the interaction between transcription factor YY1 and DNA G4 structures, which contributes to chromatin looping induced by YY1 dimerization as well as its transcriptional regulation.

    • Lin Li
    • , Preston Williams
    • , Wendan Ren
    • , Michelle Y. Wang
    • , Zi Gao
    • , Weili Miao
    • , Ming Huang
    • , Jikui Song
    •  & Yinsheng Wang
  • Article |

    Tryptolinamide (TLAM) is a small molecule compound that inhibits phosphofructokinase-1 activity and rescues the metabolic defects of patient-derived induced pluripotent stem cell lines with mutated mitochondrial DNA.

    • Hiroki Kobayashi
    • , Hideyuki Hatakeyama
    • , Haruna Nishimura
    • , Mutsumi Yokota
    • , Sadafumi Suzuki
    • , Yuri Tomabechi
    • , Mikako Shirouzu
    • , Hiroyuki Osada
    • , Masakazu Mimaki
    • , Yu-ichi Goto
    •  & Minoru Yoshida
  • Article |

    A two-cell setup containing tryptophanase, a flavin-dependent monooxygenase and a regiospecific halogenase (linked to a flavin reductase as a solubility tag) enables the production of 6,6'-dibromoindigo and other indigoid dyes in Escherichia coli.

    • Jeongchan Lee
    • , Joonwon Kim
    • , Ji Eun Song
    • , Won-Suk Song
    • , Eun-Jung Kim
    • , Yun-Gon Kim
    • , Hee-Jin Jeong
    • , Hye Rim Kim
    • , Kwon-Young Choi
    •  & Byung-Gee Kim
  • Article |

    The authors identified a pH-dependent protonated status in the miR-21 precursor, which leads to additional base pairing in its secondary structure, thus affecting Dicer processing and miR-21 maturation.

    • Jared T. Baisden
    • , Joshua A. Boyer
    • , Bo Zhao
    • , Scott M. Hammond
    •  & Qi Zhang
  • Article |

    The helical bundle structure of the CC1 domain of STIM1 of the store-activated calcium channel CRAC is crucial to maintaining the channel resting state, and helix–helix interactions can be manipulated to normalize a disease-linked STIM1 mutant.

    • Petr Rathner
    • , Marc Fahrner
    • , Linda Cerofolini
    • , Herwig Grabmayr
    • , Ferdinand Horvath
    • , Heinrich Krobath
    • , Agrim Gupta
    • , Enrico Ravera
    • , Marco Fragai
    • , Matthias Bechmann
    • , Thomas Renger
    • , Claudio Luchinat
    • , Christoph Romanin
    •  & Norbert Müller
  • Article |

    Structural and kinetic analyses of the transcriptional repressor SqrR in multiple states indicate that its persulfide selectivity is determined by structural frustration in the disulfide form, favoring formation of the tetrasulfide-bridged product.

    • Daiana A. Capdevila
    • , Brenna J. C. Walsh
    • , Yifan Zhang
    • , Christopher Dietrich
    • , Giovanni Gonzalez-Gutierrez
    •  & David P. Giedroc
  • Article |

    Screening for substrate preference of the SARS-CoV and SARS-CoV-2 main protease Mpro leads to the development of activity-based probes useful for structural analysis and for visualization of active Mpro in infected patient epithelial cells.

    • Wioletta Rut
    • , Katarzyna Groborz
    • , Linlin Zhang
    • , Xinyuanyuan Sun
    • , Mikolaj Zmudzinski
    • , Bartlomiej Pawlik
    • , Xinyu Wang
    • , Dirk Jochmans
    • , Johan Neyts
    • , Wojciech Młynarski
    • , Rolf Hilgenfeld
    •  & Marcin Drag
  • Article |

    A screening approach finds VH-domain antibodies that bind the SARS-CoV-2 Spike protein receptor-binding domain at its interface with host ACE2. Bi-paratopic and multivalent binders have high affinity and potency.

    • Colton J. Bracken
    • , Shion A. Lim
    • , Paige Solomon
    • , Nicholas J. Rettko
    • , Duy P. Nguyen
    • , Beth Shoshana Zha
    • , Kaitlin Schaefer
    • , James R. Byrnes
    • , Jie Zhou
    • , Irene Lui
    • , Jia Liu
    • , Katarina Pance
    • , Caleigh M. Azumaya
    • , Julian R. Braxton
    • , Axel F. Brilot
    • , Meghna Gupta
    • , Fei Li
    • , Kyle E. Lopez
    • , Arthur Melo
    • , Gregory E. Merz
    • , Frank Moss
    • , Joana Paulino
    • , Thomas H. Pospiech Jr.
    • , Sergei Pourmal
    • , Cristina Puchades
    • , Alexandrea N. Rizo
    • , Amber M. Smith
    • , Ming Sun
    • , Paul V. Thomas
    • , Feng Wang
    • , Zanlin Yu
    • , Daniel Asarnow
    • , Julian R. Braxton
    • , Melody G. Campbell
    • , Cynthia M. Chio
    • , Un Seng Chio
    • , Miles Sasha Dickinson
    • , Devan Diwanji
    • , Bryan Faust
    • , Meghna Gupta
    • , Nick Hoppe
    • , Mingliang Jin
    • , Fei Li
    • , Junrui Li
    • , Yanxin Liu
    • , Gregory E. Merz
    • , Henry C. Nguyen
    • , Joana Paulino
    • , Thomas H. Pospiech Jr.
    • , Sergei Pourmal
    • , Smriti Sangwan
    • , Raphael Trenker
    • , Donovan Trinidad
    • , Eric Tse
    • , Kaihua Zhang
    • , Fengbo Zhou
    • , Caleigh M. Azumaya
    • , Christian Billesboelle
    • , Alisa Bowen
    • , Melody G. Campbell
    • , Devan Diwanji
    • , Nick Hoppe
    • , Yen-Li Li
    • , Phuong Nguyen
    • , Carlos Nowotny
    • , Cristina Puchades
    • , Mali Safari
    • , Smriti Sangwan
    • , Kaitlin Schaefer
    • , Amber M. Smith
    • , Raphael Trenker
    • , Tsz Kin Martin Tsui
    • , Natalie Whitis
    • , Jianhua Zhao
    • , Daniel Asarnow
    • , Caleigh M. Azumaya
    • , Cynthia M. Chio
    • , Bryan Faust
    • , Meghna Gupta
    • , Kate Kim
    • , Michelle Moritz
    • , Tristan W. Owens
    • , Joana Paulino
    • , Jessica K. Peters
    • , Sergei Pourmal
    • , Kaitlin Schaefer
    • , Tsz Kin Martin Tsui
    • , Justin Biel
    • , Ishan Deshpande
    • , Nadia Herrera
    • , Huong T. Kratochvil
    • , Xi Liu
    • , Ursula Schulze-Gahmen
    • , Iris D. Young
    • , Jen Chen
    • , Amy Diallo
    • , Loan Doan
    • , Sebastian Flores
    • , Meghna Gupta
    • , Mingliang Jin
    • , Huong T. Kratochvil
    • , Victor L. Lam
    • , Yang Li
    • , Megan Lo
    • , Gregory E. Merz
    • , Joana Paulino
    • , Aye C. Thwin
    • , Erron W. Titus
    • , Zanlin Yu
    • , Fengbo Zhou
    • , Yang Zhang
    • , David Bulkley
    • , Arceli Joves
    • , Almarie Joves
    • , Liam McKay
    • , Mariano Tabios
    • , Eric Tse
    • , David A. Agard
    • , Yifan Cheng
    • , James S. Fraser
    • , Adam Frost
    • , Natalia Jura
    • , Tanja Kortemme
    • , Nevan J. Krogan
    • , Aashish Manglik
    • , Oren S. Rosenberg
    • , Daniel R. Southworth
    • , Robert M. Stroud
    • , Kliment A. Verba
    • , Xin X. Zhou
    • , Kevin K. Leung
    •  & James A. Wells
  • Article |

    The plant cuticle was initially thought to act as a passive diffusion barrier. Genetic and metabolic analysis reveals that it is also a sink/concentrator for volatiles protecting cells from toxic effects of these hydrophobic compounds.

    • Pan Liao
    • , Shaunak Ray
    • , Benoît Boachon
    • , Joseph H. Lynch
    • , Arnav Deshpande
    • , Scott McAdam
    • , John A. Morgan
    •  & Natalia Dudareva
  • Article |

    Increased production of (S)-reticuline and other alkaloids is achieved through alleviating norcoclaurine synthase toxicity by targeting the enzyme to the peroxisome plus enlarging peroxisomes by expression of engineered transcription factors.

    • Parbir S. Grewal
    • , Jennifer A. Samson
    • , Jordan J. Baker
    • , Brian Choi
    •  & John E. Dueber
  • Article |

    A family of riboswitches named Sensei that specifically sense iron has been discovered and characterized, which enables the authors to engineer metal ion sensing riboswitches to convert their metal selectivity.

    • Siladitya Bandyopadhyay
    • , Susmitnarayan Chaudhury
    • , Dolly Mehta
    •  & Arati Ramesh
  • Article |

    A chemical glycobiology approach reveals that heparan-sulfate glycosaminoglycans regulate vascular development through direct interactions with angiopoietin (Ang) ligands and the Tie1 receptor of the Ang–Tie signaling system.

    • Matthew E. Griffin
    • , Alexander W. Sorum
    • , Gregory M. Miller
    • , William A. Goddard III
    •  & Linda C. Hsieh-Wilson
  • Article |

    Structural analysis of transcription activation complex comprising E. coli transcription factor CueR, RNAP holoenzyme and promoter DNA reveals that CueR distorts the DNA conformation to promote the association of promoter with polymerase.

    • Chengli Fang
    • , Steven J. Philips
    • , Xiaoxian Wu
    • , Kui Chen
    • , Jing Shi
    • , Liqiang Shen
    • , Juncao Xu
    • , Yu Feng
    • , Thomas V. O’Halloran
    •  & Yu Zhang
  • Article |

    The whitefly Bemisia tabaci defends against plant glucosinolate toxins by serial addition of glucose moieties catalyzed by a pair of glycoside hydrolases, preventing toxin activation during feeding on the plant tissue.

    • Osnat Malka
    • , Michael L. A. E. Easson
    • , Christian Paetz
    • , Monika Götz
    • , Michael Reichelt
    • , Beate Stein
    • , Katrin Luck
    • , Aleksa Stanišić
    • , Ksenia Juravel
    • , Diego Santos-Garcia
    • , Lilach L. Mondaca
    • , Simon Springate
    • , John Colvin
    • , Stephan Winter
    • , Jonathan Gershenzon
    • , Shai Morin
    •  & Daniel G. Vassão
  • Article |

    A fluorescence-based sensor of PKA activity has increased brightness, dynamic range and signal-to-noise ratio over related sensors and is useful for visualizing kinase activity in HeLa cells, primary neurons and the cortex of awake mice.

    • Jin-Fan Zhang
    • , Bian Liu
    • , Ingie Hong
    • , Albert Mo
    • , Richard H. Roth
    • , Brian Tenner
    • , Wei Lin
    • , Jason Z. Zhang
    • , Rosana S. Molina
    • , Mikhail Drobizhev
    • , Thomas E. Hughes
    • , Lin Tian
    • , Richard L. Huganir
    • , Sohum Mehta
    •  & Jin Zhang
  • Article |

    Native ion mobility mass spectrometry reveals two isoforms of the two-pore domain K+ channel K2P4.1 have distinct binding preferences for lipids and show a relationship between the strength of individual lipid binding events and channel activity.

    • Samantha Schrecke
    • , Yun Zhu
    • , Jacob W. McCabe
    • , Mariah Bartz
    • , Charles Packianathan
    • , Minglei Zhao
    • , Ming Zhou
    • , David Russell
    •  & Arthur Laganowsky
  • Article |

    A bifunctional AURORA-A degrader induces the fast and specific degradation of this kinase in cancer cell lines, which enables targeting of non-catalytic, oncogenic functions of AURORA-A resulting in S-phase arrest and rampant apoptosis.

    • Bikash Adhikari
    • , Jelena Bozilovic
    • , Mathias Diebold
    • , Jessica Denise Schwarz
    • , Julia Hofstetter
    • , Martin Schröder
    • , Marek Wanior
    • , Ashwin Narain
    • , Markus Vogt
    • , Nevenka Dudvarski Stankovic
    • , Apoorva Baluapuri
    • , Lars Schönemann
    • , Lorenz Eing
    • , Pranjali Bhandare
    • , Bernhard Kuster
    • , Andreas Schlosser
    • , Stephanie Heinzlmeir
    • , Christoph Sotriffer
    • , Stefan Knapp
    •  & Elmar Wolf
  • Article |

    Immunomodulatory drugs are used for the treatment of multiple myeloma. ARID2, a component of the PBAF chromatin-remodeling complex, is a new pomalidomide-induced neosubstrate of CRL4CRBN, which accounts for its superior efficacy over lenalidomide.

    • Junichi Yamamoto
    • , Tetsufumi Suwa
    • , Yuki Murase
    • , Shumpei Tateno
    • , Hirotaka Mizutome
    • , Tomoko Asatsuma-Okumura
    • , Nobuyuki Shimizu
    • , Tsutomu Kishi
    • , Shuji Momose
    • , Masahiro Kizaki
    • , Takumi Ito
    • , Yuki Yamaguchi
    •  & Hiroshi Handa
  • Article |

    Imaging of phosphatidylcholine, sphingomyelin and their interorganelle lipid transport in live cells, using azido-choline and a spatially limited bioorthogonal tag, suggests that autophagosomal membranes originate from the ER.

    • Tomonori Tamura
    • , Alma Fujisawa
    • , Masaki Tsuchiya
    • , Yuying Shen
    • , Kohjiro Nagao
    • , Shin Kawano
    • , Yasushi Tamura
    • , Toshiya Endo
    • , Masato Umeda
    •  & Itaru Hamachi
  • Article |

    Cryo-EM structural work shows sterols binding at four adjacent locations within the class F GPCR Smoothened (SMO), where the transmembrane core functions as a sterol tunnel in which occupancy activates SMO for downstream Hedgehog signaling.

    • Xiaofeng Qi
    • , Lucas Friedberg
    • , Ryan De Bose-Boyd
    • , Tao Long
    •  & Xiaochun Li
  • Article |

    Incorporation of the non-canonical amino acid 3-aminotyrosine into the chromophores of green fluorescent protein-based biosensors systematically red-shifts their fluorescent properties while maintaining brightness, dynamic range and responsiveness.

    • Shen Zhang
    •  & Hui-wang Ai
  • Article |

    Reengineering of the lac operon in E. coli from a ligand-inducible to a blue-light-regulated gene expression system facilitates optogenetic control of biotechnological applications including metabolic engineering and protein expression.

    • Makoto A. Lalwani
    • , Samantha S. Ip
    • , César Carrasco-López
    • , Catherine Day
    • , Evan M. Zhao
    • , Hinako Kawabe
    •  & José L. Avalos
  • Article |

    Pyruvate-responsive circuits based on an orthologous transcription factor and adaptation of an antisense transcriptional circuit were developed to sense pyruvate in Bacillus subtilis and redirect metabolism for optimized glucaric acid production.

    • Xianhao Xu
    • , Xueliang Li
    • , Yanfeng Liu
    • , Yonglian Zhu
    • , Jianghua Li
    • , Guocheng Du
    • , Jian Chen
    • , Rodrigo Ledesma-Amaro
    •  & Long Liu
  • Article |

    An irreversible small-molecule inhibitor of histone methyltransferase NSD1 is developed, which binds covalently to the C2062 residue in the catalytic SET domain and represses H3K36 dimethylation and target gene expression in leukemia cells.

    • Huang Huang
    • , Christina A. Howard
    • , Sergei Zari
    • , Hyo Je Cho
    • , Shirish Shukla
    • , Hao Li
    • , Juliano Ndoj
    • , Paula González-Alonso
    • , Caroline Nikolaidis
    • , Joshua Abbott
    • , David S. Rogawski
    • , Mykhaylo A. Potopnyk
    • , Katarzyna Kempinska
    • , Hongzhi Miao
    • , Trupta Purohit
    • , Andrew Henderson
    • , Anna Mapp
    • , Maria Luisa Sulis
    • , Adolfo Ferrando
    • , Jolanta Grembecka
    •  & Tomasz Cierpicki
  • Article |

    A cell-free system for cannabinoid production uses only low-cost inputs with 12 enzymes and can operate either aerobically or anaerobically, in addition to reducing ATP requirements by use of an engineered system for malonate-CoA biosynthesis.

    • Meaghan A. Valliere
    • , Tyler P. Korman
    • , Mark A. Arbing
    •  & James U. Bowie
  • Article |

    Structural and biochemical analysis of E. coli transketolase with 2′-methoxy-thiamine shows that this antivitamin selectively inhibits the bacterial enzyme via a steric clash with a critical glutamate residue, preventing cofactor activation.

    • Fabian Rabe von Pappenheim
    • , Matteo Aldeghi
    • , Brateen Shome
    • , Tadhg Begley
    • , Bert L. de Groot
    •  & Kai Tittmann
  • Article |

    Cryo-EM structural work defines binding of the insecticide CHL in the pseudo-voltage-sensor domain of ryanodine receptor RyR that triggers conformational changes leading to channel opening and explains the resistance to CHL by some insects.

    • Ruifang Ma
    • , Omid Haji-Ghassemi
    • , Dan Ma
    • , Heng Jiang
    • , Lianyun Lin
    • , Li Yao
    • , Arthur Samurkas
    • , Yuxin Li
    • , Yiwen Wang
    • , Peng Cao
    • , Shian Wu
    • , Yan Zhang
    • , Takashi Murayama
    • , Bernard Moussian
    • , Filip Van Petegem
    •  & Zhiguang Yuchi