Volume 19 Issue 4, April 2017

Volume 19 Issue 4

Stem cells. This multi-scale, single-cell study reveals that human haematopoietic stem cells continuously commit to individual blood cell lineages without passing through discrete progenitor stages. [article p271]

Editorial

  • Editorial |

    Data sharing is an inherent principle of research publication, and information on how data may be accessed is key for the replication and furthering of scientific findings. Here we revisit the policies of Nature Cell Biology on data availability.

News and Views

  • News & Views |

    Determining the differentiation potential of stem and progenitor cells is essential for understanding their function, yet our ability to do so is limited by the restrictions of experimental assays. Based on single-cell functional and molecular profiling experiments, a new computational approach shows how lineage commitment may occur in human haematopoiesis.

    • Fiona K. Hamey
    •  & Berthold Göttgens
  • News & Views |

    Although the mitochondrial inner membrane rhomboid peptidase PARL is known to participate in critical signalling cascades, its role in apoptosis has remained unclear. PARL is now shown to process the mitochondrial pro-apoptotic protein Smac (also known as DIABLO) for its subsequent release into the cytosol where it antagonizes XIAP-mediated caspase inhibition to promote apoptosis.

    • Naotada Ishihara
    •  & Katsuyoshi Mihara
  • News & Views |

    Genetic mutations in the SHANK family of proteins are linked to multiple neuropsychiatric disorders including autism spectrum disorders. A study now elucidates critical roles for SHANK in regulating integrin-mediated cell–extracellular matrix adhesion, by sequestering integrin activators.

    • Paul Atherton
    •  & Christoph Ballestrem
  • News & Views |

    The Hippo pathway responds to environmental factors including nutrient availability, cell density and substrate stiffness to regulate organ size. This pathway is now shown to also regulate antiviral defence by modulating the TBK1-mediated control of interferon production.

    • Natalia Muñoz-Wolf
    •  & Ed C. Lavelle

Articles

  • Article |

    Velten et al. use single-cell transcriptomics and functional data to map the early lineage commitment of human haematopoietic stem cells as a continuous process of cells passing through transitory states rather than demarcating discrete progenitors.

    • Lars Velten
    • , Simon F. Haas
    • , Simon Raffel
    • , Sandra Blaszkiewicz
    • , Saiful Islam
    • , Bianca P. Hennig
    • , Christoph Hirche
    • , Christoph Lutz
    • , Eike C. Buss
    • , Daniel Nowak
    • , Tobias Boch
    • , Wolf-Karsten Hofmann
    • , Anthony D. Ho
    • , Wolfgang Huber
    • , Andreas Trumpp
    • , Marieke A. G. Essers
    •  & Lars M. Steinmetz
  • Article |

    Chia et al. show that developmental arrest after human nuclear transfer is associated with genetic instability, including DNA damage, which occurs during DNA replication and is influenced by the developmental origin of the transferred nucleus.

    • Gloryn Chia
    • , Judith Agudo
    • , Nathan Treff
    • , Mark V. Sauer
    • , David Billing
    • , Brian D. Brown
    • , Richard Baer
    •  & Dieter Egli
  • Article |

    Lilja et al. find that SHANK proteins inhibit the signalling of Ras and Rap G-proteins by restricting their availability at the plasma membrane. This leads to restricted integrin activation, affecting cell spreading, migration and invasion.

    • Johanna Lilja
    • , Thomas Zacharchenko
    • , Maria Georgiadou
    • , Guillaume Jacquemet
    • , Nicola De Franceschi
    • , Emilia Peuhu
    • , Hellyeh Hamidi
    • , Jeroen Pouwels
    • , Victoria Martens
    • , Fatemeh Hassani Nia
    • , Malte Beifuss
    • , Tobias Boeckers
    • , Hans-Juergen Kreienkamp
    • , Igor L. Barsukov
    •  & Johanna Ivaska
  • Article |

    Zebrafish neuroectoderm morphogenesis is influenced by the mesoderm germ layer. Smutny et al. now show that friction forces between cells moving in opposite directions, mediated by E-cadherin adhesion, determine the position of the neural anlage.

    • Michael Smutny
    • , Zsuzsa Ákos
    • , Silvia Grigolon
    • , Shayan Shamipour
    • , Verena Ruprecht
    • , Daniel Čapek
    • , Martin Behrndt
    • , Ekaterina Papusheva
    • , Masazumi Tada
    • , Björn Hof
    • , Tamás Vicsek
    • , Guillaume Salbreux
    •  & Carl-Philipp Heisenberg
  • Article |

    Miao et al. show through imaging and mathematical simulations that changing the activation thresholds in an excitable PIP2–Ras–Rap signal transduction network can alter the types of protrusions formed in migrating Dictyostelium cells.

    • Yuchuan Miao
    • , Sayak Bhattacharya
    • , Marc Edwards
    • , Huaqing Cai
    • , Takanari Inoue
    • , Pablo A. Iglesias
    •  & Peter N. Devreotes
  • Article |

    Donato et al. show that Fbxo15 targets acetylated KBP for degradation to limit mitochondrial expansion, whereas KBP accumulation promotes mitochondrial biogenesis in a Kif1Bα-dependent manner.

    • Valerio Donato
    • , Massimo Bonora
    • , Daniele Simoneschi
    • , Davide Sartini
    • , Yasusei Kudo
    • , Anita Saraf
    • , Laurence Florens
    • , Michael P. Washburn
    • , Matthias Stadtfeld
    • , Paolo Pinton
    •  & Michele Pagano
  • Article |

    Zhang et al. show that YAP and TAZ suppress nucleic acid sensing and antiviral responses by inhibiting the TBK1 kinase. Conversely, Hippo pathway activity inactivates YAP/TAZ to relieve TBK1 suppression and enhance antiviral defence in zebrafish.

    • Qian Zhang
    • , Fansen Meng
    • , Shasha Chen
    • , Steven W. Plouffe
    • , Shiying Wu
    • , Shengduo Liu
    • , Xinran Li
    • , Ruyuan Zhou
    • , Junxian Wang
    • , Bin Zhao
    • , Jianming Liu
    • , Jun Qin
    • , Jian Zou
    • , Xin-Hua Feng
    • , Kun-Liang Guan
    •  & Pinglong Xu

Letters

Erratum