Volume 38 Issue 6, June 2020

Volume 38 Issue 6

Cas13d guide design principles

Optimizing guide RNA design for Cas13. With data from massively parallel screens, Wessels et al. develop computational models to identify optimal guide RNAs for the RNA-targeting CRISPR enzyme Cas13d.

See Wessels et al.

Image: Christian Stolte. Cover Design: Erin Dewalt.

Editorial

  • Editorial |

    Insufficient SARS-CoV-2 testing has left many nations flying blind as they reopen their economies. Without adequate testing, new infections could outrun control measures.

News

Correspondence

Features

    Patents

  • Patents |

    Recent patents related to vaccines and methods of treatment of coronaviruses.

News & Views

Research

    Letters

  • Letter |

    Millions of primary IgG-secreting cells from mouse and human are characterized for activity and antibody sequence at the single-cell level.

    • Annabelle Gérard
    • , Adam Woolfe
    • , Guillaume Mottet
    • , Marcel Reichen
    • , Carlos Castrillon
    • , Vera Menrath
    • , Sami Ellouze
    • , Adeline Poitou
    • , Raphaël Doineau
    • , Luis Briseno-Roa
    • , Pablo Canales-Herrerias
    • , Pascaline Mary
    • , Gregory Rose
    • , Charina Ortega
    • , Matthieu Delincé
    • , Sosthene Essono
    • , Bin Jia
    • , Bruno Iannascoli
    • , Odile Richard-Le Goff
    • , Roshan Kumar
    • , Samantha N. Stewart
    • , Yannick Pousse
    • , Bingqing Shen
    • , Kevin Grosselin
    • , Baptiste Saudemont
    • , Antoine Sautel-Caillé
    • , Alexei Godina
    • , Scott McNamara
    • , Klaus Eyer
    • , Gaël A. Millot
    • , Jean Baudry
    • , Patrick England
    • , Clément Nizak
    • , Allan Jensen
    • , Andrew D. Griffiths
    • , Pierre Bruhns
    •  & Colin Brenan
  • Articles

  • Article |

    The protein complexes associated with specific chromatin marks in living cells are identified using engineered binding proteins.

    • Rodrigo Villaseñor
    • , Ramon Pfaendler
    • , Christina Ambrosi
    • , Stefan Butz
    • , Sara Giuliani
    • , Elana Bryan
    • , Thomas W. Sheahan
    • , Annika L. Gable
    • , Nina Schmolka
    • , Massimiliano Manzo
    • , Joël Wirz
    • , Christian Feller
    • , Christian von Mering
    • , Ruedi Aebersold
    • , Philipp Voigt
    •  & Tuncay Baubec
  • Analysis

  • Analysis |

    Seven methods for single-cell RNA sequencing are benchmarked on cell lines, primary cells and mouse cortex.

    • Jiarui Ding
    • , Xian Adiconis
    • , Sean K. Simmons
    • , Monika S. Kowalczyk
    • , Cynthia C. Hession
    • , Nemanja D. Marjanovic
    • , Travis K. Hughes
    • , Marc H. Wadsworth
    • , Tyler Burks
    • , Lan T. Nguyen
    • , John Y. H. Kwon
    • , Boaz Barak
    • , William Ge
    • , Amanda J. Kedaigle
    • , Shaina Carroll
    • , Shuqiang Li
    • , Nir Hacohen
    • , Orit Rozenblatt-Rosen
    • , Alex K. Shalek
    • , Alexandra-Chloé Villani
    • , Aviv Regev
    •  & Joshua Z. Levin
  • Analysis |

    A multicenter study compares 13 commonly used single-cell RNA-seq protocols.

    • Elisabetta Mereu
    • , Atefeh Lafzi
    • , Catia Moutinho
    • , Christoph Ziegenhain
    • , Davis J. McCarthy
    • , Adrián Álvarez-Varela
    • , Eduard Batlle
    • , Sagar
    • , Dominic Grün
    • , Julia K. Lau
    • , Stéphane C. Boutet
    • , Chad Sanada
    • , Aik Ooi
    • , Robert C. Jones
    • , Kelly Kaihara
    • , Chris Brampton
    • , Yasha Talaga
    • , Yohei Sasagawa
    • , Kaori Tanaka
    • , Tetsutaro Hayashi
    • , Caroline Braeuning
    • , Cornelius Fischer
    • , Sascha Sauer
    • , Timo Trefzer
    • , Christian Conrad
    • , Xian Adiconis
    • , Lan T. Nguyen
    • , Aviv Regev
    • , Joshua Z. Levin
    • , Swati Parekh
    • , Aleksandar Janjic
    • , Lucas E. Wange
    • , Johannes W. Bagnoli
    • , Wolfgang Enard
    • , Marta Gut
    • , Rickard Sandberg
    • , Itoshi Nikaido
    • , Ivo Gut
    • , Oliver Stegle
    •  & Holger Heyn

Amendments & Corrections

  • Author Correction |

    • Jiarui Ding
    • , Xian Adiconis
    • , Sean K. Simmons
    • , Monika S. Kowalczyk
    • , Cynthia C. Hession
    • , Nemanja D. Marjanovic
    • , Travis K. Hughes
    • , Marc H. Wadsworth
    • , Tyler Burks
    • , Lan T. Nguyen
    • , John Y. H. Kwon
    • , Boaz Barak
    • , William Ge
    • , Amanda J. Kedaigle
    • , Shaina Carroll
    • , Shuqiang Li
    • , Nir Hacohen
    • , Orit Rozenblatt-Rosen
    • , Alex K. Shalek
    • , Alexandra-Chloé Villani
    • , Aviv Regev
    •  & Joshua Z. Levin
  • Author Correction |

    • Annabelle Gérard
    • , Adam Woolfe
    • , Guillaume Mottet
    • , Marcel Reichen
    • , Carlos Castrillon
    • , Vera Menrath
    • , Sami Ellouze
    • , Adeline Poitou
    • , Raphaël Doineau
    • , Luis Briseno-Roa
    • , Pablo Canales-Herrerias
    • , Pascaline Mary
    • , Gregory Rose
    • , Charina Ortega
    • , Matthieu Delincé
    • , Sosthene Essono
    • , Bin Jia
    • , Bruno Iannascoli
    • , Odile Richard-Le Goff
    • , Roshan Kumar
    • , Samantha N. Stewart
    • , Yannick Pousse
    • , Bingqing Shen
    • , Kevin Grosselin
    • , Baptiste Saudemont
    • , Antoine Sautel-Caillé
    • , Alexei Godina
    • , Scott McNamara
    • , Klaus Eyer
    • , Gaël A. Millot
    • , Jean Baudry
    • , Patrick England
    • , Clément Nizak
    • , Allan Jensen
    • , Andrew D. Griffiths
    • , Pierre Bruhns
    •  & Colin Brenan
  • Publisher Correction |

    • Joo Guan Yeo
    • , Martin Wasser
    • , Pavanish Kumar
    • , Lu Pan
    • , Su Li Poh
    • , Fauziah Ally
    • , Thaschawee Arkachaisri
    • , Amanda Jin Mei Lim
    • , Jing Yao Leong
    • , Liyun Lai
    • , Kee Thai Yeo
    • , Elene Seck Choon Lee
    • , Camillus Jian Hui Chua
    • , Anis Larbi
    • , Ma Shwe Zin Nyunt
    • , Tze Pin Ng
    • , Sabrina Chiesa
    • , Marco Gattorno
    • , Alberto Martini
    • , Bhairav Suryakant Paleja
    • , Charles-Antoine Dutertre
    • , Jinmiao Chen
    • , Katherine Nay Yaung
    • , Swee Ping Tang
    • , Sue Kheng Ng
    • , Chee Fu Yung
    • , Angela Yun June Tan
    • , Shu Ying Lee
    • , Florent Ginhoux
    •  & Salvatore Albani

Careers & Recruitment

    Career Feature

  • Career Feature |

    Though academic institutions have contributed enormously to the remarkable growth in the healthcare industry, a key limiting resource for this continued growth is the creation of career opportunities for doctoral graduates.

    • Sinje Gehr
    • , Craig C. Garner
    •  & Karin N. Kleinhans
  • People

  • People |

    Recent moves of note in and around the biotech and pharma industries.