Series |

Series on CRISPR technology

Advances in CRISPR-based systems have greatly expanded the molecular toolbox for biologists. In this Series, we present commissioned Perspective and Review articles that highlight the progress made using CRISPR–Cas9 technology and its relevance for cell biological research. In addition, this online collection also features related research published in Nature Cell Biology. The Series will be updated as new primary and commissioned content is being published.

Nature Cell Biology Reviews & Perspectives

In this Perspective, Lea and Niakan describe advances in CRISPR/Cas9 genome editing techniques and discuss ethical questions and potential clinical implications of this technology.

Perspective | | Nature Cell Biology

Editorial

Advances in CRISPR-based systems have greatly expanded the molecular toolbox for biologists. In this issue, we present the first of a Series of commissioned Review articles that highlight the progress made using CRISPR–Cas9 technology and its relevance for cell biological research.

Editorial | | Nature Cell Biology

Related Nature Cell Biology Research & Comment

CRISPR base editors can induce single-base-pair changes in the genome, although they are often inefficient. A study now shows that fusion of the DNA-binding domain of RAD51 to base editors enhances both the efficiency and the targeting range of optimized enzymes. These ‘hyper-editors’ offer effective tools for disease modeling and gene therapy.

News & Views | | Nature Cell Biology

MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.

News & Views | | Nature Cell Biology

Functional genetic screening of mice and other mammals is exceedingly challenging. A CRISPR-based mutagenesis screen in mice has successfully revealed amino acids vital for protein function of the DND1 gene, missense mutations of which lead to defects in primordial germ cell development.

News & Views | | Nature Cell Biology