Functional genetic screening of mice and other mammals is exceedingly challenging. A CRISPR-based mutagenesis screen in mice has successfully revealed amino acids vital for protein function of the DND1 gene, missense mutations of which lead to defects in primordial germ cell development.
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Serebrenik, Y.V., Shalem, O. CRISPR mutagenesis screening of mice. Nat Cell Biol 20, 1235–1237 (2018). https://doi.org/10.1038/s41556-018-0224-y
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DOI: https://doi.org/10.1038/s41556-018-0224-y