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Temperature alters Plasmodium blocking by Wolbachia

Abstract

Very recently, the Asian malaria vector (Anopheles stephensi) was stably transinfected with the wAlbB strain of Wolbachia, inducing refractoriness to the human malaria parasite Plasmodium falciparum. However, conditions in the field can differ substantially from those in the laboratory. We use the rodent malaria P. yoelii and somatically transinfected An. stephensi as a model system to investigate whether the transmission blocking potential of wAlbB is likely to be robust across different thermal environments. wAlbB reduced malaria parasite prevalence and oocyst intensity at 28°C. At 24°C there was no effect on prevalence but a marked increase in oocyst intensity. At 20°C, wAlbB had no effect on prevalence or intensity. Additionally, we identified a novel effect of wAlbB that resulted in reduced sporozoite development across temperatures, counterbalancing the oocyst enhancement at 24°C. Our results demonstrate complex effects of temperature on the Wolbachia-malaria interaction and suggest the impacts of transinfection might vary across diverse environments.

Introduction

Despite intensive control efforts, malaria remains one of the most important infectious diseases worldwide1. Manipulation of mosquitoes to render them ineffective at transmitting parasites and pathogens could create exciting new opportunities for control of vector-borne diseases e.g.2,3,4,5,6. One of the most promising approaches involves transinfection of mosquitoes with strains of the maternally inherited endosymbiotic bacteria, Wolbachia (reviewed in7,8,9,10,11,12). Studies with a range of mosquito and parasite/pathogen species have shown Wolbachia to reduce vector competence and/or vectorial capacity4,13,14,15,16,17,18,19,20,21,22,23,24,25,26. Further, Wolbachia possesses a genetic drive mechanism (cytoplasmic incompatibility) that enhances its own spread through host populations by skewing normal Mendelian inheritance ratios27. Research on the use of Wolbachia for control of dengue has progressed to the level of controlled field releases of transinfected Aedes aegypti mosquitoes8,28. Research on malaria mosquito vectors has proven more challenging, but a stably transinfected line of Anopheles stephensi has recently been developed and evaluated in the laboratory4.

The mechanisms underlying the transmission blocking properties of Wolbachia remain unclear, but appear linked to aspects of mosquito immune function4,15,19,20,21,29,30,31 and possible competition for resources within the mosquito15. Recent research has demonstrated that mosquito immune function can vary considerably with modest changes in temperature32,33,34. It is also well established that growth of malaria parasites within the mosquito is strongly temperature-dependent35. Furthermore, studies from a wide diversity of non-vector systems illustrate that Wolbachia replication, dissemination, vertical transmission, fitness effects and the extent of cytoplasmic incompatibility can all vary with temperature (SI Table 1). This range of thermal sensitivities raises the possibility that patterns of Wolbachia-induced transmission blocking might depend strongly on the local environment. If so, insights gained under standard insectary conditions at 27°C may reveal little about the natural environments for malaria transmission where mean temperatures can range from 18 to 34°C and daily temperature variation frequently exceeds 10°C36,37,38. For example, blocking could be enhanced under certain conditions, increasing the effectiveness of the approach. Alternatively, variation in temperature might reduce blocking. The worst-case scenario is that malaria infection might actually be enhanced in certain environments by the interaction between Wolbachia and Plasmodium39.

Table 1 Generalized linear model analysis of the effect of experimental treatment on wAlbB density, oocyst prevalence and oocyst intensity

As proof of principle, we use a rodent malaria P. yoelii and somatically transinfected An. stephensi as a model to investigate how changes in temperature influence the Wolbachia-malaria parasite interaction. If temperature significantly influences pathogen blocking, such effects would suggest that the impacts of transinfection cannot be determined from studies conducted under one set of conditions alone.

Results

Effects of temperature on Wolbachia density and mosquito survival

The density of Wolbachia increased over time (Fig. 1, Table 1), with mosquitoes sampled on day 20 exhibiting significantly higher densities of wAlbB than on days 16 (p < 0.0001), 12 (p < 0.0001) and 8 (p < 0.0001) post-injection. Wolbachia densities were also significantly higher in the warmer temperatures of 26°C and 28°C, compared with cooler temperatures (20°C vs. warmer temperatures, p < 0.0001; 22°C vs. 24°C, p = 0.017; 22°C vs. 26°C and 28°C, p < 0.0001; 24°C vs. warmer temperatures, p < 0.0001; Table 1). A significant interaction between sampling day and temperature (days post-Wolbachia infection x temperature) indicated that the rates of wAlbB replication increased with warming temperatures, with no significant increases in wAlbB densities occurring at 20°C and 22°C (Fig. 1).

Figure 1
figure 1

Temperature, sampling time point and their interaction significantly influenced wAlbB replication in whole mosquito carcasses.

wAlbB density (ratio of wAlbB to host rpS7 genomes) is clearly mediated by temperature, with the rate of replication significantly increasing in mosquitoes housed at 26°C compared to those housed at 24°C and no significant changes through time in wAlbB densities occurring in mosquitoes housed at 20°C and 22°C. Bars around mean values represent standard errors.

There was no significant effect of temperature or Wolbachia infection on mosquito survival (Supplementary Information Text S2). There was a minor transient effect of micro-injection on mortality within the first 24-48 hrs and this was consistent between the wAlbB and Sua5B control treatments (Supplementary Information Text S2Fig. 1; Sua5B cell control: X2 = 106.28, p < 0.0001); wAlbB: X2 = 113.48, p < 0.0001). After the first two days of the experiment, the Sua5B control and wAlbB survival curves resemble that of the unmanipulated population (Supplementary Information Text S2).

Effects of temperature on Plasmodium blocking

There was an effect of temperature on the probability of a mosquito becoming infected with P. yoelii (Table 1, Fig. 2a), with oocyst prevalence being significantly lower at 28°C (p < 0.0001) compared with cooler temperatures. There was no additional effect of Wolbachia on parasite prevalence. However, temperature and Wolbachia did interact to impact oocyst intensity (Table 1, Fig. 2b). At 20°C, the number of oocysts per mosquito midgut did not differ between treatments. At 24°C, infection with wAlbB significantly facilitated the establishment of oocysts, such that the transinfected mosquitoes had more than double the oocyst burdens of the Sua5B control and unmanipulated control mosquitoes (Sua5B lysate, p < 0.0001; unmanipulated, p = 0.01). At 28°C on the other hand, infection with wAlbB limited the number of establishing oocysts, reducing oocyst burdens by approximately 80% relative to control groups (Sua5B lysate, p < 0.0001; unmanipulated, p < 0.0001).

Figure 2
figure 2

Temperature shaped the wAlbB-malaria interaction in complex ways.

(a) Temperature alone significantly affected oocyst prevalence (the proportion of mosquitoes with oocysts on their midguts), with significant declines in oocyst prevalence occurring at 28°C for all treatment groups (asterisk represents significant pairwise comparisons for each treatment group at 28°C with treatment groups placed at lower temperatures, p < 0.05). (b) Temperature significantly mediated the effect of wAlbB on oocyst intensity (the number of oocysts per midgut). wAlbB infection either enhanced (24°C), blocked (28°C), or had no effect on the number of establishing oocysts (20°C). (c) Infection with wAlbB significantly reduced the number of sporozoites produced per oocyst across all temperature treatments. (d) Temperature significantly mediated the effect to wAlbB on the total number of sporozoites produced per mosquito, with significant declines in overall sporozoite production at 28°C in wAlbB infected mosquitoes. Bars represent the unadjusted means of each response variable, while whiskers portray the standard error around the mean. Asterisks in (b–d) denote significant pair-wise comparisons within a temperature between wAlbB and the other treatment groups. The asterisk within parentheses at 24°C indicates a significant effect of wAlbB infection in explaining variation between the estimated marginal means in the full statistical model, but there is no significant effect when the unadjusted treatment means are compared.

We next examined the number of sporozoites per oocyst to provide a measure of parasite replication rate. Infection with wAlbB significantly reduced the number of sporozoites produced per oocyst across all temperatures, irrespective of Wolbachia infection status (Table 2, Fig. 2c). In general there was a relationship between the number of oocysts per midgut (oocyst intensity) and the number of sporozoites produced per oocyst. This negative effect of oocyst density was most marked in the transinfected mosquitoes and temperatures sub optimal for parasite development (20°C and 28°C). This was especially the case for wAlbB infected mosquitoes at 28°C (Regression on model residuals: B = −0.203, R2 = 0.867, F1,8 = 21.111, p = 0.002), as illustrated by a significant interaction between treatment and oocyst intensity observed at 28°C (Table 2).

Table 2 Generalized linear model analysis of the effect of treatment on the number of sporozoites produced per oocyst and midgut analyzed independently for each experimental temperature

Finally, we used total sporozoites per mosquito midgut as a measure of overall infection intensity (and hence ultimate transmission potential). With this overall measure, GZLM model analyses predicted that infection with wAlbB significantly reduced the total number of sporozoites produced at 24°C (unmanipulated vs. wAlbB, p < 0.0001; Sua5B lysate vs. wAlbB, p < 0.0001) and 28°C (unmanipulated vs. wAlbB, p < 0.0001; Sua5B lysate vs. wAlbB, p = 0.002; Table 2) relative to the controls and no significant effect of Wolbachia on malaria infection at 20°C (Table 2). However, when comparing the unadjusted means at 24°C to model estimates, we did not observe a significant difference in total sporozoite production in the wAlbB treatment group relative to the controls (Fig. 2d). This is most likely because the majority of the variance in the model is explained by the positive relationship between oocyst intensity and sporozoites produced per midgut. Thus, while the contribution of treatment in the model is small, it still significantly predicts the remaining variation unexplained by our covariate, with wAlbB infected mosquitoes producing fewer sporozoites (Supplementary Information Text S2). This result indicates that enhancement of oocyst intensity by wAlbB at 24°C was more or less counterbalanced by the negative effects wAlbB infection on parasite replication rate.

At 28°C wAlbB caused significant reductions in overall infection intensity since both numbers of oocysts and sporozoite replication were negatively affected (Sua5B cells, p = 0.003; unmanipulated, p = 0.010; Table 2, Fig. 2d). We did see replicate effects at one temperature for both the sporozoite per oocyst and sporozoite per midgut analyses, likely due to variation in infection intensities between individual mice (Table 2). However, there were no significant interactions between replicate and temperature or treatment, so the replicate effects appear to have little influence on the results overall.

Discussion

Here we use a rodent malaria and somatically transinfected An. stephensi as a model to investigate for the first time how changes in temperature influence the Wolbachia-malaria parasite interaction. We show that temperature significantly affects Wolbachia replication in the mosquito vector and alters the extent and apparent mode of action of transmission blocking. Temperature affected the replication kinetics of Wolbachia, establishment and replication of the malaria parasite and the Wolbachia-parasite interaction. Infection with wAlbB reduced oocyst intensity at 28°C, increased oocyst intensity at 24°C and had no effect at 20°C. Oocyst intensity is a common measure used to estimate parasite transmission blocking21,39. In this context, our results demonstrate that somatic infection with wAlbB can partly block, enhance, or have no impact on infection depending on temperature. However, wAlbB also appears to interfere with parasite replication, reducing the number of sporozoites produced per oocyst. When these effects are combined, wAlbB reduces transmission potential strongly at 28°C but has no overall effect at 20°C, or the thermal optimum for P. yoelii development, 24°C. The potential for such marked temperature-dependence in transmission blocking phenotypes has not been considered previously.

Use of a rodent malaria complicates direct extension of our results to human malaria. Successful sporozoite invasion of the salivary glands by P. yoelii is inconsistent, especially at 28°C35, which is why we used sporozoite load within the midgut as our measure of overall infection intensity. Quantification of sporozoite intensity within the salivary glands, as is more reliable with P. falciparum, would provide a more definitive measure of pathogen blocking. We also used somatically infected mosquitoes rather than the recently developed stable transinfected line. While there has been little research to date comparing somatic infection and stable transinfection with Wolbachia, previous studies indicate that apart from the ovarian tissues, the density and distribution of Wolbachia within host tissues is similar between somatic and stable infections, both infection strategies impair development of P. falciparum at the oocyst stage4,21 and modulate similar expression levels of immune genes4,20,21,29,31. Further, the establishment and development rate of both human and rodent malarias are temperature sensitive, even though the absolute thermal performance profiles differ between species. Similar to earlier work investigating the effects of temperature on P. yoelii35, we found negative effects of temperature on P. yoelii oocyst establishment in the midgut at 28°C. We would not expect P. falciparum oocyst establishment to be negatively affected until temperatures exceed 30°C40. However, this is not an unusual temperature for An. stephensi to experience in the field37. Moreover, P. vivax, which is the other key species of human malaria transmitted by An. stephensi, has a lower temperature threshold than P. falciparum37. Accordingly, there is little reason to believe the influence of temperature to be unique to our model system.

We found replication of wAlbB to be temperature sensitive, with an apparent optimum between 26°C and 28°C. Studies in numerous systems show Wolbachia replication to increase towards some thermal optimum and then decline as temperatures increase further (Supplementary Information Table S1). Consistent with previous research conducted on An. gambiae, we found no effect of somatic infection on mosquito mortality, irrespective of whether or not a mosquito received a bloodmeal21. At 28°C, we found a reduction in oocyst prevalence and intensity due to wAlbB. These results are similar to those reported for P. falciparum in the stably transinfected line of An. stephensi at 27°C4. At cooler temperatures we found no effects on prevalence but an increase in oocyst intensity (significant at 24°C). This is in accord with studies on another rodent malaria, P. berghei, which showed no impact on prevalence, but enhancement of oocyst intensities in somatically transinfected An. gambiae housed at 19°C39.

The mechanisms underpinning the diverse transmission blocking phenotypes require further study. There is some evidence that wAlbB induces the production of reactive oxygen species (ROS), as well as the upregulation of immune genes like TEP1 and LIRM-120 and that this can inhibit initial Plasmodium infection4,21. How this would lead to enhancement under certain temperatures is unclear but the timing of midgut microbiota proliferation, ROS production, immune gene expression and parasite kinetics directly following the blood meal might be crucial for parasite blocking at early stages of malaria infection. These different processes are all likely temperature sensitive but need not show identical responses34. In addition, the rodent malarias might initiate different responses since ookinetes typically form and migrate through the midgut more rapidly (12–24 hr post-infection) than P. falciparum (48 hr)41. Other elements of mosquito immune response also differ between rodent and human malaria. The Toll pathway primarily regulates mosquito defense at the oocyst stage against rodent malarias, while the IMD pathway is important for defense against P. falciparum42,43,44. Such differences might lead to contrasting patterns of blocking depending on which genes are modulated by wAlbB infection and how immune mechanisms are affected by temperature21. Variation in wAlbB densities within the mosquito could also play a role, although previous research on somatically infected An. gambiae suggests there is no direct relationship between oocyst burdens and wAlbB densities for either P. berghei or P. falciparum21,39.

The impact of wAlbB infection on sporozoite development is a completely novel finding. Overall, wAlbB reduced the number of sporozoites produced per oocyst across experimental temperatures. This effect counterbalanced the substantial enhancement of oocysts at 24°C leading to no net impact of wAlbB on overall infection intensity, while further strengthening blocking at 28°C. The link with oocyst density suggests that the effect could be due in part to direct or indirect resource-mediated competition with sporozoite replication inhibited when wAlbB and/or malaria parasite density are high45. The presence of Wolbachia, combined with host resource demands, could limit the amount or accessibility of resources available for Plasmodium development15. Wolbachia and Plasmodium are amino acid heterotrophs and have important and potentially overlapping lipid requirements46,47,48,49. Whether Wolbachia infection further affects the viability and invasibility of Plasmodium sporozoites needs to be further investigated in this system.

In this study we demonstrate complex effects of ambient temperature on the Wolbachia-malaria interaction that alter the nature and extent of pathogen blocking. Mean temperatures and daily temperature ranges in natural transmission environments can far exceed those considered here. Based on our data it would be remarkable if the interactions between Wolbachia, the mosquito vector and human malaria parasites were unaffected by such variation. Moreover, the impact of wAlbB on species such as P. vivax or on mixed infections remains unexplored; the potential for enhancement need not be limited to rodent malaria. Further development of this promising control technology requires an improved understanding of how mosquitoes, Wolbachia and malaria parasites will interact in diverse transmission settings.

Methods

Mosquito rearing, experimental design and infections

We reared Anopheles stephensi (Liston) under standard insectary conditions at 26 ± 0.5°C, 80% humidity and a 12 h light: 12 h dark photo-period and on a 10% glucose solution diet32. Wolbachia (wAlbB, isolated from in vitro cultivation of Wolbachia pipientis in an Ae. albopictus cell line50) was cultured and extracted from wAlbB-infected Sua5B Anopheles cells and suspended in Schneider's insect cell media as previously described31,51,52. On day three post-emergence, adult female mosquitoes were randomly allocated to one of three treatment groups: 1) unmanipulated, 2) injected with 0.2 μL Sua5B cell lysates and Schneider's insect cell media, or 3) injected with 0.2 μL of Wolbachia (5 × 106Wolbachia per mL; 1,000 bacteria per dose). Mosquitoes that were challenged with Sua5B cell lysates or Wolbachia received intrathoracic injection into the anepisternal cleft32 with a mouth pipette and microcapillary glass needle. Immediately after injection, 1200 mosquitoes from each treatment group (3600 mosquitoes total) were placed into cages (20 × 20 × 20 cm, 80 mosquitoes per cage) and randomly distributed across five Percival incubators set to different experimental temperatures (20°C, 22°C, 24°C, 26°C and 28°C ± 0.5°C, 80% humidity at a 12 hr light: 12 hr dark photo-period) and three replicates. These experimental temperatures reflect a realistic temperature range for P. yoelii transmission, which has a thermal optimum around 24°C for parasite transmission and development35,53.

To maintain experimental tractability, on day eight post-infection with Wolbachia, mosquitoes from each treatment group and replicate that were housed across three experimental temperatures (20°C, 24°C and 28°C) received a malaria infectious bloodmeal from a mouse (C57 BI/6, >6 weeks old) infected with 105Plasmodium yoelii parasites (clone 17XNL, from the WHO Registry of Standard Malaria Parasites, University of Edinburgh, UK) four days prior. Individuals that did not feed were removed from the cage. Mosquitoes were maintained at 24°C, the thermal optimum for Plasmodium yoelii development and replication35, for two hours following the 30 min bloodfeed to allow for gamete and zygote formation. After infection, individuals were placed back into their respective temperature treatments and provided with cotton balls moistened with 10% glucose offered ad libitum. Throughout the duration of the experiment, we counted and removed dead mosquitoes in each cage daily to quantify the effects of treatment and temperature on daily mosquito mortality.

Quantifying the effects of temperature on Wolbachia dissemination

We destructively sampled 10 mosquitoes per cage across all temperatures on days 8, 12, 16 and 20 post-Wolbachia infection. Mosquitoes were killed and immediately frozen at −80°C for future qPCR analyses. DNA was extracted from whole mosquito carcasses using the insect supplement in the 96-well DNeasy Blood and Tissue kit (Qiagen, Inc.) as per the manufacturer's protocol. We performed all qPCR analyses on an ABI Prism 7500 Sequence Detection System (TaqMan) using the Rotor-Gene SYBR Green PCR kit as per the manufacturer's protocol (Qiagen, Inc.) and the following cycler conditions (40 cycles total: activation, 95°C for 5 min; denaturation, 40 cycles run at 95°C for 5 sec each; and annealing and extension, 60°C for 30 sec). wAlbB was amplified from mosquito carcasses with modified GF and BR primers which specifically bind to the wsp gene54. The relative abundance of wAlbB strain was determined after normalization to the mosquito single-copy rpS7 gene21,32. All qPCRs were completed in triplicate and the average efficiency of each assay was determined by quantifying the slope of a standard curve of five 1:10 serial dilutions from a positive sample in duplicate. Wolbachia densities (presented as a fold-change relative to rpS7 DNA) were generated using the qGENE software55.

Quantifying the effects of temperature on Plasmodium blocking

We compared how oocyst prevalence, intensity and sporozoite replication varied with Wolbachia infection and temperature (20°C, 24°C and 28°C). To quantify parasite prevalence and intensity, we removed and immediately killed 20 mosquitoes from each treatment and temperature group with chloroform and dissected their midguts in 1× phosphate-buffered saline solution under a standard dissecting scope. Using a compound microscope, we noted whether a midgut was infected or uninfected and counted the number of Plasmodium oocysts that had established in each infected midgut. Midguts were then immediately stored in 95% ethanol for future molecular analyses to assess Plasmodium replication and potential sporozoite production. To account for thermal shifts in Plasmodium development rates35, dissections were staggered to ensure midguts were sampled at approximately the same stage of oocyst development (day 7, 8 and 10 post-malaria infection for 24°C, 28°C and 20°C, respectively).

Due to inconsistent salivary gland invasion by P. yoelii sporozoites, we used sporozoite production per midgut and per oocyst as proxies to estimate transmission potential. To assess sporozoite production, DNA was extracted from individual midguts collected during oocyst dissection using the E.Z.N.A. MicroElute Genomic DNA kit (Omega Bio-Tek, as per the manufacturer's protocol). DNA was eluted in 20 μL of elution buffer and the number of parasite genomes present in midguts was quantified using a previously developed qPCR assay56. Sporozoite production per oocyst was evaluated by dividing the total number of sporozoites per midgut by the number of oocysts quantified for each midgut.

Statistical analyses

All statistical analyses for these experiments were run in IBM SPSS Statistics 21.0 (IBM Corporation). Full factorial models from generalized linear model (GZLM) analysis were reduced through backward elimination of non-significant interactions. We assessed goodness of fit of the final models through model deviance, log likelihood values and model residuals. Covariates included in GZLMs were centred on their grand mean and adjusted Bonferroni post-hoc tests were used to identify significant pair-wise comparisons. For all dependent variables analyzed, we included the following factors in our model analysis: temperature (Wolbachia densities and mosquito survival, 20°C, 22°C, 24°C, 26°C and 28°C; Plasmodium blocking, 20°C, 24°C and 28°C), treatment (unmanipulated, Sua5B and wAlbB) and replicate.

We used a GZLM assuming a normal distribution to determine how Wolbachia density was affected by our experimental treatment groups. In addition to the fixed factors mentioned above, we included sampling date post-Wolbachia infection in our model analysis (day 8, 12, 16 and 20 PI). We used a Poisson GZLM to quantify the effects of experimental treatment on the average number of infected mosquitoes and a negative binomial (log link function) GZLM to quantify the effects of experimental treatment on oocyst intensity. Because of differences in parasite replication rates due to temperature, we ran a series of GZLM analyses (assuming either a normal distribution on transformed sporozoite data or a gamma distribution on untransformed data) on the total number of Plasmodium sporozoites per midgut and per oocyst independently for each temperature. Because midguts with more oocysts likely produce more sporozoites, we also included oocyst intensity as a covariate in both analyses. Finally, we used Kaplan-Meier survival analyses to generate cumulative daily survival estimates to determine the effects of temperature and treatment on daily mosquito survival throughout the course of the experiment.

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Acknowledgements

We thank members of the Thomas and Read lab groups for discussion and J. Teeple for insectary support and R. Schneider for assistance with Wolbachia culture and purification. This research was funded by the following grants: NSF-NIH EID (EF-0914384), NIH-R21 (AI096036-01), NIH-R21 (AI070178) and NIH-R21 (AI088311). The content of this paper is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases and the National Institutes of Health. This project was also assisted by a grant from the Pennsylvania Department of Health using Tobacco Settlement Funds. The Department specifically disclaims responsibility for any analyses, interpretations or conclusions.

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C.C.M. contributed to conceiving and designing experiments, running the experiments, analyzing data associated with the experiments and in writing the manuscript. S.B. contributed to designing and running the experiments. G.L.H. contributed to designing the experiment, provided tools and techniques for the experiment and in writing the manuscript. J.L.R. contributed by providing resources for the experiment and in writing the manuscript. M.B.T. contributed to conceiving the experiments, providing resources for the experiments and in writing the manuscript.

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Murdock, C., Blanford, S., Hughes, G. et al. Temperature alters Plasmodium blocking by Wolbachia. Sci Rep 4, 3932 (2014). https://doi.org/10.1038/srep03932

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