Abstract
The conversion of carbon dioxide to medium-chain fatty acids (CO2-to-MCFAs) through microbial processes represents a valuable technology for sequestering and exploiting CO2, generating superior bio-chemicals from the primary contributor to the greenhouse effect. However, a comprehensive overview and generalization of microbial CO2-to-MCFAs are presently deficient. Based on this, the present review systematically summarizes the research progress, explicates the process mechanisms, analyses the key challenges and possible solutions, and anticipates forthcoming research perspectives and priorities for the first time. We proposed two original strategies, namely the synchronous strategy and integrated strategy, from current research into microbial CO2-to-MCFAs. The synchronous strategy concurrently achieves hydrogen (H2) and CO2 assimilation, as well as MCFAs production, by employing a reactor that co-cultivates predominant H2/CO2-utilizing microorganisms and chain elongation microorganisms. The integrated approaches involve CO2-to-precursors (i.e., acetate and ethanol) and subsequent precursors-to-MCFAs, achieved through the use of two bioreactors for separately cultivating H2/CO2-utilizing microorganisms and chain elongation microorganisms. Mechanistic insights reveal that microbial CO2-to-MCFAs predominantly encompasses two processes: H2 and CO2 assimilation into precursor and subsequent precursors chain elongation into MCFAs, through a Wood-Ljungdahl pathway and a two-round elongation, respectively. The analyses of key challenges and possible solutions for microbial CO2-to-MCFAs underscore the imperative to enhance efficiency and economy and to shed light on metabolic mechanisms. Furthermore, in order to improve the strategy application potential of microbial CO2-to-MCFAs, future research perspectives and priorities, e.g. exploitation of functional pure bacteria, screening of functional pure bacteria, multi-omics analysis, genetic modification and enhancement, enhancement of bioreactor stability, specific MCFA production, development of coupled purification technology for MCFAs, and economic benefits and ecological environmental risks, are proposed and prospected. This work is expected to offer a thorough understanding of the microbial CO2-to-MCFAs, guide and inspire researchers to address critical challenges in-depth and propel the development of CO2-to-MCFAs.
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Introduction
With the development of human society, the global energy demand is growing rapidly, with more than 80% of the need being met by fossil fuels, e.g., coal, petroleum, natural gas, etc. However, the global carbon dioxide (CO2) released from fossil fuel combustion is now approximately 36.8 billion tons yearly1. If the current uptrend continues, the CO2 fossil-fuel-related emissions load is likely to double by mid-century, which will cause global warming of about 2 °C2,3. This trajectory of climate change entails an array of potential risks associated with climate destabilization, encompassing adverse natural phenomena, glacier melt, and intensified occurrences of extreme weather patterns. Confronted with the dual challenges of escalating energy demand and the exacerbation of greenhouse climate effect, CO2 capture, utilization, and storage (CCUS) has garnered considerable attention in recent years due to its pivotal role in mitigating the impact of global warming4,5,6.
In comparison to CO2 storage, the conversion and utilization of CO2 represent a noteworthy renewable energy approach, holding the potential to alleviate CO2 emissions and diminish reliance on fossil fuels, thereby contributing significantly to combating global warming. Presently, a growing number of CO2 utilization technologies, including chemical catalysts7,8, photochemical9,10, electrochemical11,12, as well as biogenic source such as photoautotrophic microalgae13,14 and chemoautotrophic CO2-utilizing bacterium15,16, are undergoing development and practical implementation. These technologies facilitate the conversion of CO2 into a range of renewable energy sources, spanning bio-fuels, bio-chemicals, high-molecular compounds, and degradable plastics. As illustrated in Fig. 1, the bio-products based-CO2 involve in methane (CH4)17, alcohols18, carboxylates19,20, biodiesel21, glucose22, polyhydroxybutyrate23, and starch24, single-cell protein25, and analogous substances.
Among these bio-products derived from CO2, carboxylates, consisting of short-chain fatty acids (SCFAs, with 1-5 carbon chain, e.g., acetate and propionate) and medium-chain fatty acids (MCFAs, with 6-12 carbon chain, e.g., caproate and heptylate), are an extremely versatile bio-chemicals. Notably, MCFAs, serving as high value-added platform compounds, can be directly used as feed additives, plant growth promoters, and antimicrobials or serve as precursors for a broad spectrum of commodities, such as aviation fuel, lubricants, fragrances, paint additives, and pharmaceuticals26,27. Furthermore, MCFAs exhibit diminished dissolution properties (caproate of 93.1 mmol L−1, heptylate of 18.5 mmol L−1, caprylate of 4.9 mmol L−1, pelargonate of 1.1 mmol L−1, caprate, undecanoate and dodecanoate are almost insoluble in water, at 25 °C and 1.01 × 105 Pa), allowing MCFAs much more straightforward to separate from liquid effluent. This inherent property of lower solubility gives MCFAs a significant advantage in lowering separation costs.
This review aims to present a comprehensive overview of chemoautotrophic CO2-utilizing bacterium for CO2 conversion into MCFAs. Our focus is on summarizing the progress of microbial CO2-to-MCFAs and elucidating the underlying strategies and mechanisms involved. Additionally, the challenges and possible solutions in this process are addressed, and future perspectives and priorities in this field are discussed.
Progress of microbial CO2-to-MCFAs
Current research into microbial CO2-to-MCFAs predominantly employs hydrogen (H2) or H2 proton (H+) as an electron donor to assist CO2 conversion. Additionally, a subset of studies explores the conversion of CO2 into MCFAs by using co-electron donors and acceptors including short-chain alcohols (e.g., methanol and ethanol), carboxylate (e.g., acetate, propionate, butyrate), and lactate. As delineated in Tables 1–3, these studies on microbial CO2-to-MCFAs can be systematically categorized into two principal strategies: the synchronous strategy and the integrated strategy.
Function microbial
The research into CO2-to-MCFAs primarily encompasses two categories of functional microorganisms: H2/CO2-utilizing microorganism (also known as Homoacetogen) and chain elongation microorganisms. The both microorganisms togetherly facilitate the efficient conversion of H2 and CO2 into MCFAs. In the mixed-culture bioreactors, H2/CO2-utilizing microorganism and chain elongation microorganisms play a dominant role in the microbial community. Meanwhile, numerous high-effective Homoacetogens, e.g., Acetobacterium woodii28, Clostridum ljungdahlii29, Clostridum ragsdalei P1130, Clostridum autoethanogenum31, Clostridum carboxidivorans32, etc, along with a select number of chain elongation microorganisms, e.g., Clostridum kluyveri33, have been isolated and screened for their efficacy in converting CO2 to ethanol and acetate, as well as facilitating the chain elongation process for MCFAs production in the pure-culture bioreactors.
H2 roles and sources
It is widely believed that H2 acts as electrons to drive the conversion of CO2 into acetate and ethanol within H2/CO2-utilizing microorganisms. The sources of pure H2 and H+ are derived from electrolytic water and anode of microbial electrolysis cells (MEC). The electric power is generated by surplus windmills or solar panels. In addition, the H2 utilized for driving CO2 conversion sources from syngas (a mixture of H2, CO2, CO), which has a huge capacity emanating from biomass gasification, steelmaking industry, and coke-oven plant34,35. Among these H2 supply means, MEC stands out as a low-input technology capable of overcoming the challenges associated with low-efficiency -H2 gas-liquid mass transfer. The conversion of syngas into chemicals or fuel represents a noteworthy advancement in waste gas recycling technology.
CO2 sources
The present investigation into microbial CO2-to-MCFAs showed that a predominant proportion of the CO2 utilized for MCFAs production emanates from simulated captured CO2 gas (Tables 1 and 2). The potential of CO2 for MCFAs production is very large and abundant. Currently, there exist approximately 40 commercial capture facilities globally, with a total annual capture capacity exceeding 45 million tons of CO2 (International Energy Agency, 2023). The capture of CO2 predominantly originates from large point sources, including power generation or industrial facilities that use either fossil fuels or biomass for fuel. Alternatively, the CO2 can be captured directly from the atmosphere. Some studies also use syngas to provide CO2 for MCFAs production (Tables 1 and 2). Besides, biogas comprises 35–45% CO2, attains an annual production exceeding 70 billion cubic meters (bcm) and a global potential of up to 200 bcm per year36. Biogas, therefore, can also afford a mass of CO2 for MCFAs production37. In fact, there are many more sources of CO2 that can be used to produce MCFAs.
Two strategies for CO2-to-MCFAs
The review introduces two innovative strategies in the current exploration of microbial CO2-to-MCFAs, as depicted in the schematic diagram presented in Fig. 2. The synchronous strategy employed a reactor primarily housing two collaborating microbes, namely CO2-utilizing microorganisms and chain elongation microorganisms, to facilitate simultaneous CO2 assimilation and chain elongation. In the presence of H2, H2/CO2-utilizing microorganisms and chain elongation microorganisms dominate the microbiome of a mixed-culture bioreactor, allowing for the concurrent assimilation of H2 and CO2 into acetate and ethanol and chain elongation of endogenous acetate and ethanol into MCFAs. Moreover, an accompanying strategy for H2/CO2-to-MCFAs involves the co-cultivate of pure Homoacetogen and chain elongation microorganisms (e.g., co-culture of Clostridum autoethanogenum and Clostridum kluyveri) to achieve synchronous H2/CO2 assimilation and chain elongation (Table 1). Furthermore, in the synchronous strategy for H2/CO2-to-MCFAs, external addition of electron donors (e.g., ethanol) and acceptors (e.g., acetate and propionate) are often implemented to facilitate chain elongation38,39.
In addition, in the synchronous strategy for CO2-to-MCFAs, certain studies have showed the feasibility of elongating CO2 into MCFAs without H2 assistance. In such cases, electron donors (e.g., methanol and ethanol) and acceptors (e.g., acetate and propionate) play a crucial role in the elongation process, as indicated in Table 2. Additionally, research reported that CO2 can promote the elongation of acetate and ethanol into MCFAs40. However, the underlying mechanism of converting CO2 and elongating electron donors (e.g., methanol and ethanol) and acceptors (e.g., acetate and propionate) into MCFAs remains unclear. In short, the synchronous strategy synchronously enables concurrent CO2 conversion and MCFAs production under with H2 assistance or without H2 assistance. The primary advantage of the synchronous strategy lies in its cost-effectiveness due to lower equipment investment. However, the synchronous implementation of both processes in a single reactor may lead to mutual inhibition, potentially causing reduced efficiency.
The integrated strategy for CO2-to-MCFAs involves two separate bioreactors (Fig. 2). In the first reactor, the assimilation of H2 and CO2 into precursors (acetate and ethanol) occurs within the H2/CO2-to-precursors bioreactor. The microbiome of this bioreactor is primarily composed of H2 and CO2 -utilizing microorganisms (e.g., Clostridum ljungdahlii, Clostridum autoethanogenum). Subsequently, the H2 and CO2 fermentation effluent, containing the precursors (acetate and ethanol), is fed into the chain elongation bioreactor. In this second bioreactor, dominant chain elongation microorganisms, such as Clostridum kluyveri, facilitate the elongation of acetate and ethanol into MCFAs. In the integrated strategy, the sources of H2 and CO2 primarily originate from syngas, MEC, and simulated captured CO2 gas (Table 3). The coupling approach effectively achieved the sequential objectives of CO2-to-precursors and subsequent precursors-to-MCFAs. Its primary advantage lies in separating CO2 assimilation and MCFAs production processes into distinct reaction units, ensuring system stability and enhancing overall performance.
Mechanism of CO2-to-MCFAs
Figure 3 illustrates the mechanisms of microbial CO2-to-MCFAs. In the context of H2 and CO2 as the substrate for MCFAs, H2/CO2-to-MCFAs mainly encompasses two key processes: the assimilation of H2 and CO2 into precursor (acetate and ethanol) and subsequent chain elongation of these precursors (acetate and ethanol) into MCFAs. In addition, some studies have reported a supplementary process wherein other electron donors (e.g., lactate) and electron acceptor (e.g., propionate) substrates are introduced to facilitate the production of MCFAs.
H2 and CO2-to-acetate and ethanol
Whether employing a synchronous strategy or an integration strategy for H2/CO2-to-MCFAs, the assimilation of H2 and CO2 into precursors (acetate and ethanol) stands as the primary process implemented by H2 and CO2-utilizing microorganisms. The majority of H2 and CO2 -utilizing microorganisms convert H2 and CO2 into acetate and ethanol via a wood-ljungdahl pathway according to the equation 4H2 + 2CO2→CH3COOH+2H2O ∆G=−95kJ mol−1 and 6H2 + 2CO2→C2H5OH + 3H2O ∆G = −97 kJ mol−1 36,41. These reactions are catalyzed by a series of microbial enzymes. As shown in Fig. 3 (CO2-to-acetate and ethanol), the assimilation of CO2 involves two pathways (methyl and carbonyl pathways), in which acetyl-CoA (acetyl-coenzyme A) works as a pivotal intermediate for acetate and ethanol production. In the methyl pathway, CO2 is first converted into formate with the assistance of H2, which acts as an electron donor for reducing CO2. Subsequently, formate undergoes successive transformations into formyl-tetrahydrofolate (formyl-THF), methenyl-THF, methylene-THF, methyl-THF, and further methyl-corrinoid iron-sulfur protein (methyl-CoFeSP). In the carbonyl pathway, CO2 is first reduced into CO, followed by CO, and along with methyl-CoFeSP, it is transformed into acetyl-CoA. Acetyl-CoA is eventually transformed into acetate and ethanol. In addition to producing the main metabolites of acetate and ethanol, small amounts of C3-5 (with 3-5 carbon chain fatty acids, e.g., propionate, butyrate and valerate) are also generated by Homoacetogens29,42. These by-products can also serve as precursors to be elongated into MCFAs.
Acetate and ethanol-to-MCFAs
The process of acetate and ethanol-to-MCFAs consists of two consecutive rounds of elongation implemented by chain elongation bacteria. The corresponding reaction equations are represented:as follows 4H2 + 2CO2→CH3COOH+2H2O ∆G=−95kJ mol−1 and 5C2H5OH + 5C3H7COOH→C5H11COOH + 4H2O + 2H2 ∆G = −183.5 kJ mol−1 43. Each round elongation is catalyzed by a series of microbial enzymes, and the process results in an increment oftwo carbon chains in each round. As shown in Fig. 3 (ethanol and acetate-to-butyrate and butyrate-to-MCFAs), in the first-round elongation (ethanol and acetate-to-butyrate), ethanol from H2 and CO2 assimilation is first oxidized to acetaldehyde and then to acetyl-CoA. coupled to another acetyl-CoA, acetyl-CoA undergoes successive transformations into acetoacetyl-CoA, hydroxybutyryl-CoA, crotonyl-CoA, and further butyryl-CoA, which then is converted into acetyl-CoA and butyrate with acetate formed from H2 and CO2 assimilation. The acetyl-CoA continues to participate in the elongation of the first round, while concurrently initiating the second-round elongation by coupling with butytyl-CoA. In the second-round elongation (butyrate-to-MCFAs), acetyl-CoA and butytyl-CoA undergo successive conversions into ketohexanoyl-CoA, 3-hydroxyhexanoyl-CoA, hex-2-enoyl-CoA, and further hexanoyl-CoA. Hexanoyl-CoA is then converted into caproate along with the butyrate generated from the first-round elongation. Notably, H2 is generated during both rounds of the elongation process. In the integration strategy of CO2-to-MCFAs, the H2 release means that some energy will inevitably be lost. However, in the synchronous strategy of CO2-to-MCFAs, the H2 is recycled with CO2 to produce acetate and ethanol, which prevents energy loss.
In addition, except for caproate, a few small quantities of heptylate and caprylate are also produced in the course of two consecutive rounds of elongation, the underlying mechanism of which remains to be conclusively established. In general, the elongation of first-round and second-round work in sync for acetate and ethanol-to-caproate within chain elongation bacteria. Consequently, both butyrate and caproate are produced simultaneously. However, the elongation processes of acetate, ethanol-to-heptylate, and caprylate may unfold successively within chain elongation bacteria. Upon achieving a specific yield of butyrate and caproate, the elongation into heptylate and caprylate is initiated, resulting in the production of heptylate and caprylate. Therefore, heptylate and caprylate are usually produced during the intermediate and later stages of chain elongation process44.
During the two successive rounds of elongation for acetate and ethanol-to-MCFAs, butyrate acts as an intermediate electron acceptor to produce MCFAs, while butyrate is also a significant by-product with a higher cumulative yield. For example, butyrate, acting as the only electron acceptor, along with ethanol, can also be elongated into MCFAs45. Nevertheless, the elongating process of acetate and ethanol-to-MCFAs yields a higher butyrate output. This shows that the more butyrate is elongated into MCFAs, the higher yield of MCFAs is formed. However, a common phenomenon observed in numerous studies involves the substantial accumulation of butyrate without subsequent elongation into MCFAs33,46. This butyrate accumulation reduces the yield of MCFAs. Therefore, the efficiency of butyrate-to-MCFAs is a limiting factor to MCFAs production. The aspect warrants meticulous consideration in future research endeavors.
Lactate or propionate-to-MCFAs
Lactate usually acts as an externally introduced electron donor for the production of MCFAs, and it can be oxidized either in conjunction with ethanol as a co-electron donor or as a standalone electron donor47. As shown in Fig. 3 (lactate as ED), lactate is initially oxidized to pyruvate, subsequently progressing to further oxidation to form acetyl-CoA. Acetyl-CoA then enter the elongation process of the first and second rounds, ultimately yielding MCFAs. Notably, a portion of CO2 is released accompanied by oxidization of pyruvate into acetyl-CoA, indicating a part loss of the carbon source when the lactate as an electron donor. In contrast, the synchronous strategy of CO2-to-MCFAs offers a distinctive advantage, as CO2 is recycled with H2 to generate acetate and ethanol, thereby preventing the loss of carbon sources. Conversely, the integration strategy of CO2-to-MCFAs lacks this particular advantage and is susceptible to carbon source loss.
Propionate typically serves as an externally added electron acceptor for MCFAs production. As shown in Fig. 3 (propionate as EA), the process involves the initial elongation of propionate, which, when coupled with acetyl-CoA, progresses to form valerate. Subsequently, valerate, in conjunction with acetyl-CoA, undergoes further elongation to produce heptylate. Consequently, propionate and valerate can function as either co-electron acceptors or distinct electron acceptors in the production of MCFAs, resulting in the generation of MCFAs with uneven carbon chains.
Key challenges and possible solutions for CO2-to-MCFAs
The key challenges for CO2-to-MCFAs primarily revolve around the low microbial conversion efficiency, resulting in an insufficient yield of MCFAs. Additionally, the economic viability of the strategy is a matter of contention, and certain aspects of the process mechanisms remain inadequately elucidated (Fig. 4). These formidable challenges have considerably impeded the seamless transition of the strategy from laboratory research to practical application.
Efficiency improvement
As shown in Tables 1–3, whether employing a synchronous or integrated strategy for H2/CO2-to-MCFAs, numerous studies supplied H2 and CO2 in the form of gases, alongside MEC providing H2 protons. However, it is essential to note that H2 and CO2 gases exhibit a lower dissolution of 0.8 mmol L−1 and 33.9 mmol L−1 under 25 °C and 1.01 × 105 Pa. Moreover, H2 and CO2 can be captured and converted by Homoacetogenes before being dissolved into the liquid phase. Therefore, the lower gas-liquid mass transfers of H2 and CO2 are a crucial limiting condition to microbial conversion efficiency in H2 and CO2-to-acetate and ethanol processes, ultimately resulting in the lower production of MCFAs. As shown in Fig. 4, to address this challenge of suboptimal H2 and CO2 gas-liquid mass transfers, all kinds of advanced reactor designs, such as hollow-fiber membrane aerated biofilm reactors and MEC48, should be developed to enhance H2 and CO2 gas-liquid mass transfer. Meanwhile, the advanced bioreactors must be capable of trapping more microbes and facilitating the timely dissociation of MCFAs. The increased retention of microbes within bioreactors is a direct and effective approach to augment the performance of CO2-to-MCFAs. Concurrently, the prompt dissociation of MCFAs is pivotal in mitigating the toxicity of MCFAs to microorganisms.
In addition, the low efficiency observed in microbial transformation of CO2-to-MCFAs in current studies is due to the inherently low metabolic activity of microorganisms. Employing bio-augmentations and powerful engineered bacteria may be effective solutions to improve the efficiency of microbial transformation for CO2-to-MCFAs. Some studies have demonstrated the effectiveness of bio-augmentations, e.g., the addition of zero-valent nano-iron (ZVNI), biochar, and activated carbon, in bolstering microbial transformation efficiency by maintaining the system stability and enhancing microbial metabolic activity49,50. Furthermore, using robust pure bacteria or genetically modified engineering bacteria have been proved to be effective means to improve the efficiency of H2 and CO2 assimilation and ethanol and acetate chain elongation29,51.
Economy improvement
While microbial approaches for CO2-to-MCFAS are gaining increasing popularity, concerns have been raised regarding their economic viability. As shown in Fig. 4, various coupled resource recovery technologies, e.g., syngas-to-biofuel, biogas upgrading, acetate and ethanol of wastewater recycling, and the like, with microbial approaches of CO2-to-MCFAs, presents a promising avenue to enhance economic feasibility. Notably, syngas-to-biofuel has been extensively documented. Additionally, the successful coupling techniques involving biogas upgrading and recycling from acetate and ethanol wastewater with CO2-to-MCFAs have also been demonstrated in previous studies52,53. For instance, synchronous biogas upgrading and MCFAs production respectively achieve greater than 96% CH4 purity and 24 mmol L−1d−1 MCFAs production rate54,55. Another study reports a feasible approach of concurrently recycling of liquor-making wastewater and yielding the high output of MCFAs47. Moreover, the utilization of cheaper mixture gases of H2 or CO2 instead of pure H2 and CO2 can contribute to reducing costs. In addition, with the rapid development of CO2 capture technology worldwide and the growing environmental benefits of CO2 reduction in response to climate change, the associated costs are expected to decrease.
Process in-depth analysis
The lack of elucidation in certain the mechanisms governing CO2-to-MCFAs represents a significant challenge, impeding its practical application. As mentioned above, the mechanism involved in converting CO2 and the elongating of electron electrons (e.g., methanol and ethanol) and acceptors (e.g., acetate and propionate) into MCFAs remains unclear. While some studies have posited that the conversion of CO2-to-MCFAs is caused by the H2 gas assistance produced by the chain elongation of ethanol and acetate56, the explicit mechanism lacks definitive confirmation. In addition, a comprehensive analysis of the mechanism governing butyrate-to-MCFAs is warranted to enhance its transformation efficiency. The large accumulation of butyrate and failure to elongate into MCFAs are also important reasons for lower yield of MCFAs.
Future perspectives
The synchronous and integrated strategy for microbial CO2-to-MCFAs holds a promising potential for CO2 recycling and MCFAs production. MCFAs, as renewable bioenergy sources, derived from waste CO2, offer an opportunity to reduce the dependence on fossil fuels and contribute to the mitigation of CO2 emissions, thereby being conducive to tackling global climate change and energy supply shortage. However, the technology of microbial CO2-to-MCFAs has yet to transition from laboratory research to practical engineering applications. As the escalation of global CO2 emissions and the increasing severity of climate change, the technology, although slow to start, is developing rapidly.
Fig. 5 shows future research perspectives and priorities aimed at boosting the application potential of CO2-to-MCFAs. The exploitation of functional pure bacteria is a the cornerstone for high-efficiency CO2-to-MCFAs. In particular, screening vital functional pure bacteria serves as a catalyst for technological advancement. The application of multi-omics, e.g., metagenome, metabolome, transcriptome, proteome, etc., is crucial for a comprehensive understanding of the metabolic pathways and transformation processes of microbial CO2-to-MCFAs. This knowledge, in turn, will pave the way for genetic modification and enhancement. With the rapid development of biotechnology, the trend in future research and development is expected to focus on genetically modified and enhanced engineered bacteria. The stability of bioreactor is paramount for practical applications, yet ensuring their prolonged stability and efficient operation remains an urgent challenge. Notably, current studies predominantly yield mixed MCFAs, including caproate, heptylate, and caprylate. However, achieving product separation and generating a specific MCFA suitable for targeted product formation presents a complex challenge. Meanwhile, the promotion of the synchronous CO2-to-MCFAs and MCFAs purification is advocated. This approach aims to achieve the simultaneous production and purification of MCFAs,, mitigating the toxic effects of MCFAs on microorganisms and stimulating the production of MCFAs. Furthermore, before practical engineering application, a meticulous assessment of economic benefits and ecological environmental risks is imperative.
In addition, future research will center on coupled resource recovery technologies, e.g., coupled syngas utilization and CO2-to-MCFAs, coupled biogas upgrading and CO2-to-MCFAs, and coupled acetate and ethanol recycle from wastewater and CO2-to-MCFAs, among others. These coupled resource recovery technologies offer a dual advantage: enhancing economic viability while simultaneously integrating and synergizing diverse process chains to achieve the production of energy products entirely from waste.
Conclusion
This review first comprehensively summarizes current research progress to microbial CO2-to-MCFAs, proposing two innovative strategies for microbial CO2-to-MCFAs, namely, synchronous strategy and integrated strategy. Then, the underlying mechanisms of the two original strategies are interpreted in detail. Moreover, a thorough analysis of the key challenges and possible solutions in microbial CO2-to-MCFAs is presented. Furthermore, future research perspectives and priorities of microbial CO2-to-MCFAs are prospected and discussed, aiming to enhance the strategy application potential. In a word, the microbial CO2-to-MCFAs emerge as a favored technology for high-value CO2 resource recovery, presenting an opportunity to reduce the dependence on fossil fuels and mitigate CO2 emissions, thus helping address global climate change and energy unsustainability.
Data availability
No datasets were generated or analysed during the current study.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. U22A20443, No. 52076063); the Fundamental Research Funds for the Central Universities (No.HIT. OCEF.2023014, No.HIT. OCEF.2021031); the Heilongjiang Provincial Natural Science Foundation of Excellent Young Scholars (Grant No. YQ2023C031); Open Project of Key Laboratory of Environmental Biotechnology, CAS (Grant No kf2020009); Heilongjiang Touyan Innovation Team Program; and the Fundamental Research Funds for the Central Universities (HIT. DZJJ.2023058); State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology) (2023DX04).
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Kai-Kai Wu: Investigation, Methodology, Validation, Writing – original draft. Pian-Pian Xu: Resources. Lei Zhao: Supervision, Writing – review & editing. Nan-Qi Ren: Supervision, writing-review & editing. Yi-Feng Zhang: Conceptualization, Supervision, Resources, Funding acquisition, Writing – review & editing.
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Wu, KK., Xu, PP., Zhao, L. et al. Microbial conversion of carbon dioxide into premium medium-chain fatty acids: the progress, challenges, and prospects. npj Mater. Sustain. 2, 4 (2024). https://doi.org/10.1038/s44296-024-00008-w
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DOI: https://doi.org/10.1038/s44296-024-00008-w