The intestine is key for nutrient absorption and for interactions between the microbiota and its host. Therefore, the intestinal response to caloric restriction (CR) is thought to be more complex than that of any other organ. Submitting mice to 25% CR during 14 days induced a polarization of duodenum mucosa cell gene expression characterised by upregulation, and downregulation of the metabolic and immune/inflammatory pathways, respectively. The HNF, PPAR, STAT, and IRF families of transcription factors, particularly the Pparα and Isgf3 genes, were identified as potentially critical players in these processes. The impact of CR on metabolic genes in intestinal mucosa was mimicked by inhibition of the mTOR pathway. Furthermore, multiple duodenum and faecal metabolites were altered in CR mice. These changes were dependent on microbiota and their magnitude corresponded to microbial density. Further experiments using mice with depleted gut bacteria and CR-specific microbiota transfer showed that the gene expression polarization observed in the mucosa of CR mice is independent of the microbiota and its metabolites. The holistic interdisciplinary approach that we applied allowed us to characterize various regulatory aspects of the host and microbiota response to CR.
The beneficial effects of caloric restriction (CR) were described as early as the 16th century (Luigi Cornaro, 1484–1566: “Discorsa della vita sobria”) and have been intensively investigated since the 1930s. However, the mechanisms behind the health outcomes of CR are not well understood. Numerous publications have consistently shown that CR increases lifespan and health span in different species regardless of sex, at least when there is little exposure to potentially life-shortening diseases1,2,3. A complex network of pathways and factors including Sirt1, mTOR, AMPK, IGF-1, FoxO, thyroid hormone, and ghrelin contribute to the overall beneficial effects of CR4. Most organs share common CR-modulated processes, but also have individual responses. The most prevalent process is the regulation of genes connected with inflammation, protein folding, metabolism, circadian rhythm, tumorigenesis, and detoxification5,6.
Surprisingly, the gastrointestinal (GI) tract, which is the first organ in contact with food, has not been well studied in the context of CR, but it is known that CR influences innate and adaptive immunity in the intestine7,8. In addition, CR also results in hypertrophy of the stomach9, but reduces cell proliferation in the duodenum and colorectal crypt cells and enterocyte differentiation10,11,12. However, there are conflicting results concerning the impact of CR on intestinal epithelial cell apoptosis11,13, villi length14,15,16, and colon weight and length11,15,17. CR does not influence intestinal permeability18, but it does counteract the age-related decline in nutrition absorption by increasing the uptake of proline, fructose, and glucose, without affecting mRNA levels of the glucose transporters14,15. Finally, CR increases the bile acid (BA) content in the intestine, which enhances lipid absorption from the intestinal lumen19.
The GI tract is singular for its crucial symbiotic interactions with a huge and diverse population of prokaryotic cells, the microbiota, and for harnessing indispensable macro- and micronutrients. Composition changes in the gut microbiota occur during weight gain and loss20,21,22,23, and the type and amount of food are the main factors shaping the gut microbiota composition23,24. An increased ratio of Firmicutes to Bacteroidetes is a hallmark of obesity25,26. Accordingly, CR has a strong influence on gut microbiota27,28.
This limited information on the intestinal response to CR prompted us to study the molecular response to CR of the duodenum mucosa and microbiota. We analysed the surface of the mucosa consisting mainly of absorptive enterocytes but also various other types of cells29. We also asked if and how the microbiota reacts and contributes to the response to CR. We discovered a clear polarisation of differentially expressed genes, with metabolic genes being upregulated and immune/inflammatory genes downregulated in the duodenum of CR mice. This gene expression dichotomisation revealed tissue-specific transcription markers of CR and their network correlated with essential changes in the faecal microbiota composition and metabolite content.
CR induces dichotomisation of the gene expression response in the duodenum mucosa
To explore the effects of CR on duodenum functions, we reduced the food intake of mice by 25% for 14 days (experimental set up is presented in Supplementary Fig. S1), which resulted in ~20% body weight loss (Supplementary Fig. S2A) and a reduction in body fat content (Supplementary Fig. S1B). The length of the small intestine (SI) and colon did not change significantly during CR (Supplementary Fig. S2C,D). However, the ratio of SI and colon length to body mass increased in CR animals, which may reflect an adaptation to increase the efficiency of nutrient uptake (Supplementary Fig. S2E,F).
Microarray-based gene expression profiling analysis of duodenum mucosa scrapings revealed 521 differentially expressed genes (DEGs) (p-value cut-off of 0.05; fold-change ≥1.5). A total of 249 genes were significantly upregulated and 272 were downregulated in CR mice compared with ad libitum mice (Supplementary Tables S1A,B).
This gene expression response to CR in the duodenum mucosa was assessed by gene ontology (GO) functional enrichment analysis30, which defined 19 enriched terms associated with metabolic processes for the upregulated genes (Figs. 1A,B, Supplementary Table S2A) and 31 terms associated with immune and inflammatory responses for the downregulated genes (Fig. 1A,B, Supplementary Table S2B). The lack of any overlapping GO term between the up- and downregulated genes suggested a strong dichotomisation of biological processes for metabolism and immunity. Furthermore, enrichment analysis of tissue-associated proteins carried out using the Uni-Prot datasets (‘UP_Tissue’) (Supplementary Table S2C) showed that the 249 metabolic associated gene subset was represented by proteins referring to small intestine, colon, kidney and liver, while the 272 immune system associated gene subset was represented by the proteins connected to jejunal and colic lymph nodes (at 87.14-fold enrichment), thymus, spleen, activated spleen, and bone marrow. In total, 96 DIGs were categorized as genes related to the immune system and 87.5% of these genes were transcriptionally downregulated. The list of these downregulated genes contains genes serving as specific markers for T-cells (Il2rg, Cd52, Atf3 and Laptm5), B-cells (Cd72, Cybasc3, Parp14, Bank1), NK-lymphocytes (Nkg7), murine macrophages (H2-q6), monocytes (Tgm2, Nkg7) and mature dendritic cells (Cxcl10, Ifit1, Tnfsf10, Atf3 and Usp18)31,32,33,34,35.
In summary, the GO analysis suggested that the functional response of the duodenum mucosa to CR is associated with dichotomised gene expression patterns referring to the immune and metabolic pathways.
MetaCore (MetaCoreTM; GeneGo Inc.) pathway enrichment analysis of the differentially expressed genes confirmed this dichotomisation (Supplementary Table S3A,B), further demonstrating that CR enhanced metabolic gene expression and reduced immune/inflammatory responses in the mouse duodenum mucosa.
Enriched interacting partners of the genes affected by CR
Next, using the MetaCoreTM gene-connectivity analysis hypergeometric test, we identified 78 genes from the entire mouse genome, which according to the literature could interact with the DEGs in the CR mouse duodenum (Supplementary Table S4A). Each of the 78 genes had a significantly higher number of interactions (literature and manual curation via MetaCoreTM) with the DEGs, when compared with the background set (i.e. all the genes from the genome), as assessed by the standard hypergeometric test (Supplementary Table S4A). A large number of them (62 out of 78) were transcription factor genes, such as Stat1/2/3/6, Irf1/2/3/7/8/9, Pparα, Pparγ, and Hnf1α/Hnf3α/Hnf3β/Hnf4α. Other genes encode enzymes such as p300 and Oas1 as well as cell membrane receptors such as Cd36, Cd74, and Tlr2. Of interest, the transcription factors Stat1/2/3/6 and Irf1/2/3/7/8/9 significantly interacted with genes downregulated under CR (Supplementary Table S4C) but not with those that were upregulated (Supplementary Table S4B). Also notable is that Stat1, Stat2, Irf1, and Irf9 were among the significantly downregulated genes in the CR mouse duodenum. In contrast, the transcription factor genes Pparα, Pparγ, and Hnf1α/Hnf3α/Hnf3β/Hnf4α significantly interacted with the upregulated probe sets, although only Pparα mRNA was significantly upregulated in the CR duodenum (Supplementary Fig. S3A). Overall, of the 57 genes that interacted with the downregulated genes, 16 (28%) were also significantly downregulated after CR, suggesting a potential tight self-regulating interconnected gene network. Furthermore, the limited overlap between the genes that interacted with upregulated vs. downregulated genes confirmed the dichotomic control of metabolic and inflammatory/immune response to CR (Supplementary Fig. S3B).
Interconnected networks of genes affected by CR
Mapping of the 521 genes differentially expressed in CR yielded 493 MetaCoreTM identified genes, which were subsequently used for network building. The MetaCoreTM network revealed a very high connectivity between genes (Supplementary Table S5). A large directly interconnected network was comprised of 119 genes (Fig. 2) with several gene hubs, such as the ISGF3 (interferon-stimulated gene factor) group comprising Stat1, Stat2, Irf9, and Irf1, and the Pparα group comprising Bmal1, Ppargc1 (Pgc1α), Srebp1 (nuclear), Spebp1 precursor, Atf-3, and Evi-1. In type 1 interferon signalling, the Stat1–Stat2 heterodimer combines with IRF9 (interferon response factor) to form a complex known as ISGF336. In fact, the most connected hub was the ISGF3 immune system gene hub, with 47, 20, 13, and 5 interactions for Stat1, Stat2, Irf9, and Isgf3, respectively. Most of the genes in this hub were downregulated after CR. The next important gene hub was the Pparα metabolic gene hub. The PPARα-interacting genes were generally upregulated after CR. The Pparα hub interacted with the Bmal1, Ppargc1 (Pgc1α), and Srebp1 hubs, which are all associated with energy metabolism. Overall, this network analysis confirmed the stimulatory and inhibitory impact of CR on the metabolic and immune systems, respectively. In addition, it revealed that PPARα and ISGF3 are likely the key transcription factors mediating this regulation.
Next, we plotted a bipartite graph of the direct interaction network (Fig. 3) to look for specific patterns of direct regulation with respect to activating or inhibiting interactions with genes exhibiting either small fold- or large fold-changes. The genes that we proposed above to be critical in the control of immune system processes, including Stat2, Irf9, and Irf1, were among those with the largest absolute fold-changes, potentially with a larger impact on downstream targets via transcriptional control.
Different parts of the GI tract show individual responses to CR
To characterise the response of the entire GI tract to CR, we performed comparative qPCR analysis of several DEGs identified by microarray analysis in the duodenum mucosa, using samples from the stomach, jejunum, ileum, and proximal and distal colon. Duodenum samples were also included for qPCR-based validation of the microarray-based observations (Fig. 4). The groups of genes tested were connected with the immune/inflammatory response (Stat1, Tlr3, Irf1, Reg3b, Reg3g, Oasl1a; Fig. 4A) and metabolism (Acot4, Acox2, Scd1, Cd36, Pparα, Vldlr; Fig. 4B). Among genes connected with immunity/inflammation (Stat1, Tlr3, Irf1), there was a significant downregulation when duodenum samples from ad libitum mice were compared to those from CR mice, confirming the microarray results. Strong responses were also observed in other parts of the SI (jejunum, ileum) compared with the stomach and colon, where they were much weaker. In addition, antimicrobial peptide (Reg3b, Reg3g, Oasl1a) gene expression was reduced during CR in all parts of the GI tract with the exception of the colon, which responded to CR by increasing transcription of Reg3b (Fig. 4A).
Genes connected with metabolism showed higher expression in the duodenum compared with colon and stomach in ad libitum fed mice. However, in response to CR, the expression of most of these genes increased not only in the duodenum, but also in the colon and stomach (Fig. 4B). Transcription of Pparα increased, in line with a previous report37, and network analysis revealed this gene was an essential regulator of the CR response (Fig. 2) and confirmed that a short 14 day CR is sufficient to induce a strong CR-like phenotype. Of interest, a simple overnight fast resulted in statistically significant CR-like gene expression changes for Stat1, Tlr3, Acot2, and Acox4 (Supplementary Fig. S3C). Altogether, these results show that gene expression in the SI differs in amplitude from that of the stomach and colon under ad libitum and CR conditions.
Response to CR in duodenum mucosa differs from that of other organs
We took advantage of published comparisons of commonly regulated genes after CR for several organs38, including the hypothalamus, liver, muscle, and colon to assess the potential specificity of the duodenum CR response. In contrast to the immune, inflammatory, and defence processes that were mostly downregulated in the duodenum, DNA packaging, nucleosome and chromatin assembly, and the biosynthesis of cholesterol, lipids, steroids, and sterol were the most downregulated processes in the colon, liver, muscle, and hypothalamus (Supplementary Fig. S4A). Duodenum shared a very limited number (14 upregulated and 5 downregulated) of CR-regulated genes with the colon, liver, muscle, and hypothalamus (Supplementary Fig. S4B,C) showing a strong tissue specific gene expression response to CR. These findings were confirmed by comparison with other recent data39. There was minimum or no overlap between microarray DEGs identified in different tissues (skeletal muscles, white adipose tissue, heart and brain neocortex)39 with those identified in duodenum mucosa samples in this study (Supplementary Figure S4D–H). These observations show that the duodenum mucosa presents a singular CR gene expression signature, most likely reflecting unique interactions among the duodenum, microbiota and food.
The mTOR pathway regulates metabolic gene expression in a CR-like manner
The most strongly upregulated (8.5 fold change, p ≤ 0.001) gene in the CR duodenum gene expression microarray data set was Fkbp5 (Supplementary Table S1A). FKBP5 forms a complex with rapamycin and acts as an inhibitor of mTOR40,41. This finding prompted us to investigate whether rapamycin would induce a duodenum CR phenotype. It did so partially by stimulating the metabolism-associated genes. However, it did not affect immunity/inflammation genes, including those encoding antibacterial peptides (Fig. 4C). Additionally, similar to CR-exposed mice, mice receiving rapamycin had lower body weight than the control mice (Supplementary Fig. S4I). We concluded that the mTOR pathway regulates the expression of metabolic genes in a CR-like manner.
CR-triggered metabolic and inflammatory gene regulation is not microbiota-dependent
Homeostasis in the intestinal tissue is orchestrated by microbiota cues that are mediated by Toll-like receptors (TLRs)42. In addition to this regulation, we found CR modifies the expression of antimicrobial peptides all along the GI tract. Taken together, these results suggest crosstalk between the intestine and the microbiota during CR. To address this question, we analysed the microbiota in both the feces and small intestine. In the feces, at the phyla level, the ratio of Firmicutes to Bacteroidetes was not drastically altered by CR, but increased diversity was observed within the families that constitute each of these phyla (Fig. 5A,B, Supplementary Figs S5 and S6). Firmicutes Clostridia Clostridiales were less dominant under CR, to the benefit of other families such as Firmicutes Bacillii (Lactobacillaceae), Clostridia (Lachnospiraceae, Ruminococcaceae) and Erysipelotrichi (Erysipelotrichaceae). Similarly, the Bacteroidetes Bacteroida S24-7 was less dominant to the benefit of Bacteroidetes Bacteroida (Bacteroidaceae, Porphyromonadaceae, and Prevotellaceae). In the small intestine, 25% of the OTUs were unassigned under ad libitum conditions and 9% under CR. Under CR, there was a decrease in Actinobacteria and Proteobacteria relative to both Firmicutes and Bacteroidetes. Moreover, the Firmicutes were dominated by Lactobacillaceae, whilst a more balanced spectrum (e.g. with Lachnospiraceae, as well as Ruminococcaceae) was observed in the ad libitum samples. S24-7 was the only assigned family for Bacteroidetes (Supplementary Figs S5 and S6). We observed a stronger impact of CR on SI bacteria than faecal bacteria. We then asked whether the microbiota has a significant role in the duodenum dichotomisation of gene expression described above. We tested this hypothesis using mice treated with antibiotics (AT) to deplete their gut flora, and then subjected them to CR. Successful depletion of microbiota in AT mice was confirmed by (i) DAPI staining of bacteria in faecal samples (Supplementary Table S6), (ii) the enlarged cecum of the treated mice, and (iii) the lack of short-chain fatty acids (SCFAs) in the CR faeces (Supplementary Fig. S7A–D); while, microbiota transfer reverted the cecum phenotype (Supplementary Fig. S7A).
Among the subsets of inflammation- and metabolism-related genes that we tested, the CR-induced gene expression changes in the duodenum mucosa were similar between AT animals and animals with a regular microbiota (Supplementary Fig. S7E). Furthermore, duodenum gene expression profiles did not differ between ad libitum mice gavaged with faecal or duodenum microbiota from CR (MT-F, MT-D) mice and from control ad libitum–fed mice (MT-FC, MT-DC; Supplementary Fig. S7E). We concluded that the duodenum mucosa regulation of metabolic and inflammatory genes triggered by CR in the duodenum mucosa is not primarily microbiota-dependent.
CR induces changes in intestinal and faecal metabolites
To characterise the metabolic consequences of CR, we analysed duodenum content (Supplementary Table S7A) and faecal samples (Supplementary Table S7B) using 1H-NMR–based metabolomics. Examples of the typical metabolic profiles obtained are displayed in Supplementary Fig. S8A,B. CR induced significant changes in duodenum metabolite content, as illustrated by the significant differences between the ad libitum and CR metabolic profiles using projection on latent structure-discriminant analysis (PLS-DA) modelling (Fig. 5C–E). Microbiota depletion attenuated the effect of CR, with a non-significant PLS-DA model between AT and AT-CR animals (Fig. 5C,F,G). In the duodenum, CR selectively increased many signals related to BA moieties, such as taurine-conjugated to BAs (triplets at 3.08 and 3.57 ppm) and CH- and -CH2 groups of BAs (multiplets between 1.2 and 2.2 ppm) (Fig. 5E). CR also increased the signals of two singlets at 0.726 and 0.73 ppm, corresponding to the C-18, C-19, and C-21 methyl groups of BAs. These signals were unambiguously assigned to cholic (CA) and taurocholic (TCA) acids by comparison with spikes in experiments of standard molecules (Supplementary Fig. S8C,D). Three other BA signals were detected in the NMR spectra of duodenum content, which were assigned to ß-muricholic acid (ßMCA), tauro-ß-muricholic acid (TßMCA), and tauroursodeoxycholic (TUDCA) acids based on previous publications43. Levels of TUDCA tended to increase upon CR (Fig. 5E,H); while, those of ßMCA, and TßMCA remained unchanged (Supplementary Fig. S8E). Accordingly, CR resulted in increased levels of ß-hydroxybutyrate in plasma (Supplementary Fig. S8E). Importantly, antibiotic treatment prevented the rise of CA, TCA, and TUDCA levels in the duodenum of AT-CR mice (Fig. 5H,I). Additionally, removal of bacteria resulted in increases in ßMCA and TßMCA levels independently of CR (Supplementary Fig. S8E). CR also decreased succinate and lactate levels in the duodenum content (Fig. 5J,K). This regulation was absent in the small intestine of CR mice with deprived microbiota.
The PLS-DA analyses conducted on faecal metabolite profiles confirmed that CR induced strong metabolite composition changes in both control and AT-treated mice (Fig. 6A–E). The analysis revealed antibiotic treatment had a very strong effect and there was significant discrimination between ad libitum and CR animals (Fig. 6C,E). CR affected SCFAs with decreased acetate, butyrate, and propionate levels (Fig. 6B, Supplementary Fig. S7B–D). Of interest, the CR-induced reduction in SCFAs was concomitant with a decrease in SCFA receptor expression in the duodenum and jejunum, but not in the colon (Fig. 6F). Faecal levels of many amino acids (branched-chain amino acids [BCAAs] - leucine, isoleucine, valine, as well as glutamine and phenylalanine), glucose and other oligosaccharides, and taurine (free) decreased under CR (Figs. 6B,G–J, Supplementary Fig. S8G,H). Most of these changes were gut microbiota-dependant since the levels of SCFAs, amino acids, and taurine were not affected by CR in AT-treated mice (Fig. 6G–I, and Supplementary Fig. S8G). Interestingly, contrary to faecal samples, the BCAA plasma concentrations increased during CR (Supplementary Fig. S8I), in line with a previously published report44. In mice lacking microbiota, CR decreased the levels of all NMR-detectable BA signals, as well as the BA-conjugated taurine signals (Fig. 6K, Supplementary Fig. S8J,K). In summary, CR induced significant changes in both duodenum and faecal metabolites, with a strong impact on BAs in the duodenum and on SCFAs and BCAAs in the faeces.
To the best of our knowledge, this is the first report to present gene expression profiling of the SI mucosa after CR. The intestine showed a tissue-specific response to CR by regulating groups of genes with selected functions. In addition, the holistic approach applied here identified a microbiota-dependent response to CR. We found that, under CR, the duodenum mucosa increased metabolism-associated gene transcription, while strongly reducing immune/inflammatory gene expression. This expression patterns most likely reflects the impact of CR on the activation of metabolic processes in the epithelial cells and possibly a reduction of the number of lymphocytes (not studied herein) or/and the suppression of immune cell activity in the duodenum mucosa.
In the intestinal mucosa, 96 immune tissue associated genes responded to CR. Among them were genes associated with T-, B- and NK-lymphocytes, which play pivotal roles in the cellular and humoral immune responses31,32,33,34,35. In the activating interaction sub-network, the genes connected with the immunity/inflammatory response were concentrated around the Isgf3 and Irf1 hubs; while, the enhanced metabolic genes were associated with Pparα, Pgc1α, Srebp1, and Bmal1. In CR, Bmal1 and other circadian clock genes are also affected in other tissues38. In fact, Bmal1 plays role in circadian rhythm as well as in metabolism. Thus, changes in its expression could reflect the affected metabolism of the host under caloric deficit. However, it may also indicate changes in the food intake pattern due to the fact that the CR mice received their daily food allowance as one meal, which they consumed rapidly. Time-restricted food intake has previously been shown to affect clock gene transcription45. Importantly, the gut microbiota itself affects the circadian rhythm of gene expression42. Disruption of the intestinal circadian clock was reported to change the uptake of water, sugars, proteins, lipids and drugs, as well as gut motility, gut serotonin and cortisol production, with an impact on the whole body42,46,47,48,49,50,51,52. In addition, PPARα and STAT1 inhibit each other at the transcriptional level53, which is consistent with our data. The upregulation of Pparα, coupled with the downregulation of Stat1, Stat2, Irf1, and Irf9 are the likely drivers of the dichotomic gene transcription observed under CR. Our bioinformatics analysis supports the hypothesis that the PPAR, STAT, IRF, and HNF families of transcription factors play critical roles in CR-induced transcriptional regulation.
To gain an overview of transcriptional regulation in response to CR, we compared our gene expression data with published microarray data sets from several organs after CR5,39. Although regulation of several immunity/inflammatory and metabolic genes has also been observed in various tissues in response to CR, the number of genes affected was much lower than found here in the duodenum mucosa. Therefore, the duodenum response is clearly distinct from that of other organs. Although decreased transcription of inflammatory genes triggered by CR is not the most prominent response to CR in organs other than the intestine mucosa, it constitutes one of the most common responses to CR among all tissues. It suggests that under CR, the whole organism saves energy by reducing its immune/inflammatory vigilance. Undernutrition, a severe form of CR, and infectious diseases caused by microorganisms have always been intricately linked, and the former is the primary cause of immunodeficiency worldwide54. Intestinal response most likely contributes to the outcome of malnutrition.
Gut microbiota–host interactions are based on mutual benefits, and several bacterial metabolites serve as signalling molecules, for instance, in shaping host immunity or in appetite regulation and energy homeostasis. It is now well established that microbiota-generated metabolites are implicated in health and disease55. An important finding of the current study in mice with depleted gut flora was that the CR-triggered changes in the expression of metabolic and immune/inflammatory genes were not significantly influenced by microbiota. However, it is likely that the changes in microbiota and their metabolites in CR are induced by the host, at least partly. Therefore, we anticipate that the strong polarisation of gene expression under CR and increased efficiency of nutrient uptake by the host would influence the microbiota composition (Fig. 7), as seen in this study for CR and previously found for restricted nutrient uptake28. This outcome is reflected by the more abundant and partially different faecal and duodenum metabolites in the ad libitum condition compared with CR.
The CR decreases BCAA in faeces, which was not observed in CR mice treated with antibiotics, implying that the CR-induced changes in BCAA almost fully depend on gut microbiota metabolism. In the colon, amino acids are intensively metabolised by microbiota, with lysine, glycine, and arginine BCAAs being the preferred substrates56. The BCAAs, as well as other AAs, that were affected by CR such as phenylalanine and glutamate, can be biosynthesized by bacteria and this process contributes significantly to the host AA pool57. Of interest, leucine, isoleucine, valine, and phenylalanine, which were upregulated in the faeces of ad libitum mice, compared with CR mice, are plasma markers that correlate with an increased diabetes risk58. However, it is unclear if the reduced levels of these amino acids contribute to the beneficial health effects of CR.
We found decreased concentrations of all SCFAs in the faeces of CR mice, an effect that was dependent on the microbiota. Of note, significantly higher concentrations of SCFAs were found in obese versus normal-weight children and reduced consumption of carbohydrates by obese subjects resulted in decreased concentrations of butyrate and butyrate-producing bacteria in faeces59,60. Butyrate and propionate stimulate intestinal gluconeogenesis by activating gluconeogenic gene expression61,62,63. Thus, shortage of butyrate and propionate during CR, or after antibiotic treatment, may impact the glucose balance in the whole body and enhance the energy deficit during CR. Moreover, decreased butyrate and propionate levels may play a signalling role in CR by preventing stimulation of adipocyte proliferation64 and attenuating signalling of satiety65,66.
A substantial part of our metabolic profiling results concerns changes in BA levels in the duodenum and faeces, which may directly impact lipid, glucose, and energy metabolism67,68. CR induced an increase of TUDCA, CA, and TCA in the SI; while, other BA-related signals remained unchanged. These observations are in line with previous reports demonstrating that CR dose-dependently increased the amount of tauro-conjugated but not unconjugated BAs. Moreover, CR selectively increased 12α-hydroxylated-BAs (i.e. BAs of the CA pathway) and decreased non-12α-hydroxylated groups (i.e. BAs from the MCA pathway) in all compartments of the enterohepatic cycle in mice19,69. Interestingly, this increased ratio of 12α-hydroxylated BAs/non-12α-hydroxylated BAs correlated with improved glucose tolerance and lipid parameters19 and is suspected to play a key role in metabolic improvement after bypass surgery in humans70. Furthermore, impaired generation of 12α-hydroxylated BAs has been associated with dyslipidaemia and insulin resistance71. However, these results are controversial since eliminating 12α-hydroxylated BAs in mice through Cyp8b1 knockout caused low body weight and improved glucose tolerance72. Additionally, antidiabetic treatment decreased the 12α-hydroxylated BA/non-12α-hydroxylated BA plasmatic ratio in humans73. Therefore, it remains unclear whether the CR-induced BA changes observed in our study mediate the beneficial effects of CR.
The herein described increase in total BAs in plasma and the SI and the increase in TCA and TDCA in the serum of CR mice were previously associated with CR19,69, but this is the first report in the context of a microbiota analysis. We demonstrated that the gut microbiota plays a key role in both the CR-induced increase in the amount of tauro-conjugated BAs and in the CR-induced changes in the relative proportions of BAs, as microbiota depletion disrupted the BA levels observed after CR. Moreover, the levels of conjugated vs. free BAs, which is dependent on the presence of microbiota, mirrored the well-established role of gut microbiota in BA conjugation74,75. We observed increased levels of TßMCA in antibiotic-treated mice. This corresponds with results of a previous comparative study in germ-free vs. conventional mice, in which TßMCA was suggested to regulate FXR activity76. Importantly, secondary BA production by microbiota is accompanied by a feedback mechanism of BAs on microbiota composition77,78. Thus, BA concentration changes during CR may reciprocally contribute to the observed microbiota composition changes. In general, the differences in metabolite composition between antibiotic-treated vs. non-treated mice, as well as between ad libitum and CR groups, were more pronounced in the faeces compared with the duodenum contents. Thus, the magnitude of the changes corresponds to bacteria abundance in duodenum and colon79, indicating a role for gut bacteria in the metabolic response to CR.
It is important to point out that our CR experimental procedure does not include dietary supplementation that would prevent malnutrition, which is a common practice80. Importantly, the chow fed during CR provides more than the minimal requirements of micronutrients for mice (Nutrient Requirements of Laboratory Animals)81. Therefore, a 25% reduction of the diet should not result in deficiency of amino acids, minerals, or vitamins. Thus, we propose that the short duration and relatively mild diet restriction applied in our study would not result in micronutrient malnutrition.
Gene expression profiling analysis of the duodenum mucosa showed that its response to CR was a strong downregulation of immunity/inflammatory genes and an upregulation of metabolic genes in the duodenum mucosa cells. Shulzhenko and colleagues82 reported that elevated inflammation in the intestine leads to impaired lipid absorption. These results combined with ours suggest a finely adjusted balance between mucosal cell metabolism and immune/inflammatory response to optimise energy management and homeostasis during CR. Moreover, the microbiota in the intestine and colon represents an essential source of metabolites, which can serve as nutrients or signalling molecules contributing to the adaptation of the host to the CR metabolic challenge. Further studies are needed to directly identify gene expression profiles of the individual cells, distinct cell types and their integrative response to CR in duodenum. Such studies would provide a better understanding of the metabolic and immune system pathways affected by CR including the roles of the gut microbiota and its metabolites in regulating the host’s physiology and pathophysiology during CR.
Materials and Methods
Animal care and experimental procedures
Male C56Bl/6 mice from in-house breed were kept under a 12-h light/12-h dark cycle in standard housing cages with corncob bedding (BioCob, Singapore). The animals were fed Diet 3436 from Irradiated Rat and Mouse Diet (Speciality Feeds, Glen Forrest, Australia) and housed with free water access. Mice aged 12 weeks were randomly divided into experimental groups of 8–10 mice, as follows: Ad lib – control ad libitum fed; CR – caloric restriction; AT – antibiotic treated; AT-CR – antibiotic treated and caloric restriction; MT-FC – ad libitum fed, antibiotic treated with microbiota transplant from the faeces of Ad lib mice; MT-F – ad libitum fed, antibiotic treated with microbiota transplant from the faeces of CR mice; MT-DC – ad libitum fed, antibiotic treated with microbiota transplant from the duodenum of Ad lib mice; MT-D – ad libitum fed, antibiotic treated with microbiota transplant from the duodenum of CR mice; Rapm – ad libitum fed, with rapamycin in drinking water. The groups did not differ significantly in body weight when starting the experimental procedures. This experimental setup is depicted in Suppl Fig. 1.
Animal food intake was measured for one week prior to the intervention to determine the amount of chow diet to be given under CR. The mice from the CR and AT-CR groups underwent 14 days of CR that consisted of an ~75% reduction of daily food intake. To deplete gut flora, mice from the AT, AT-CR, MT-C, and MT groups were repeatedly gavaged with 200 µl of an antibiotic cocktail (vancomycin 0.5 g/l, neomycin 1 g/l, ampicillin 1 g/l, metronidazole 1 g/l; all from Sigma-Aldrich, St. Louis, MO, USA). The AT and AT-CR groups were gavaged three times within 14 days of the experimental procedure, and the MT group was gavaged twice: 5 and 3 days before microbiota transplant. Microbiota depletion in the AT and AT-CR groups was confirmed by faecal sample sequencing.
After gut flora depletion, the mice from the MT groups were gavaged twice at a 2-day interval with freshly extracted duodenum or faecal microbiota from CR or Ad lib mice. To obtain inoculants, the duodenum content was squeezed out and mixed with 1 ml of sterile phosphate-buffered saline. Similarly, fresh faeces were mixed with PBS. The mixture was vortexed and centrifuged for 3 min at 1000 × g, and the isolated supernatant was immediately gavaged into MT mice. Mice were killed 14 days after the first gavage.
Mice in the Rapm group received rapamycin in drinking water for 14 days at a concentration that yielded an intake of 1.5 mg/kg body weight/day of rapamycin.
More information is available in the Supplementary materials and methods.
All animal experimentation protocols were approved by the Vaud Cantonal Authority (authorisation VD 2440), Switzerland, and by the Institutional Animal Care and Use Committee (authorisation 2015/SHS/1023) in Singapore. All experiments were carried out according to Swiss and Singaporean animal experimentation guidelines.
Total RNA from mice duodenum scrapings was isolated and purified using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands). The total RNA was measured with a NanoDrop®ND-1000 spectrophotometer, and the RNA quality was assessed using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA). For each sample, 100 ng of total RNA was amplified using the Ambion® WT Expression Kit (#4411973, Life Technologies). A total of 5.5 μg of the cDNA was fragmented and labelled using the GeneChip® WT Terminal Labeling kit (901525, Affymetrix). Affymetrix mouse gene 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) were hybridised with 2.3 μg of fragmented target at 45 °C for 16 h and washed and stained according to the protocol described in the Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007).
Sequencing the 16 S rDNA genes and metataxonomic analysis
Bacterial DNA was extracted and sequenced; the results were analysed as described previously83. In brief, DNA was extracted using a FastDNA Spin Kit for Soil (MP Biomedicals, UK). Following PCR amplification of the V4 and V5 regions of the 16 S rDNA genes, DNA sequencing was performed using the high-throughput GS FLX Titanium 454 pyrosequencing platform (the GS FLX Titanium platform)84. Analyses of the sequencing reads were performed using the Quantitative Insights Into Microbial Ecology (QIIME) pipeline. The resulting taxonomic data were analysed by principal component analysis (PCA).
1H NMR metabolomics
Faecal and duodenum extract preparations, NMR spectroscopy, and data pre-treatment were conducted as previously described83. Data were mean-centred and scaled using unit variance scaling prior to analysis using orthogonal projection on latent structure-discriminant analysis (O-PLS-DA). The 1H NMR data were used as independent variables (X matrix) and regressed against a dummy matrix (Y matrix) indicating the class of samples (ad libitum or CR)85. The O-PLS–derived model was evaluated for goodness of prediction (Q2Y value) using 8-fold cross-validation. The reliability of each model was established using a permutation test of the Y vector (1000 permutations) to determine a p-value for each Q2Y, as previously described86. Parameters of the final models are indicated in the figure legends.
To identify metabolites discriminating the animal groups, the O-PLS-DA correlation coefficients (r2) were calculated for each variable and back-scaled into a spectral domain, so that the shape of the NMR spectra and the sign of the coefficients were preserved87. The weights of the variables were color-coded according to the square of the O-PLS-DA correlation coefficients. Correlation coefficients extracted from significant models were filtered so that only significant correlations above the threshold defined by Pearson’s critical correlation coefficient (p < 0.05; |r| > 0.49) were considered significant. For illustration purposes, the area under the curve of several signals of interest was integrated, and statistical significance was tested using t-tests or one-way ANOVA. More information is provided in the Supplementary materials and methods. All authors had access to the study data and reviewed and approved the final manuscript.
The microarray datasets generated during and analyzed during the current study are available in the ArrayExpress repository, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6248. The sequencing results are deposited in the NCBI data base.
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The authors would like to acknowledge the help of the staff at the Metabolic Evaluation Facility and Genomic Technologies Facility, as well as of Leonore Wigger from Vital-IT (all at the Center for Integrative Genomics, University of Lausanne), and Arlaine Amoyo-Brion from the National Cancer Center in Singapore. The authors also thank Cécile Canlet from the French National Infrastructure of Metabolomics and Fluxomics (MetaboHUB-ANR-11-INBS-0010) in Toulouse for her help with the NMR analyses and Olivier Cloarec from Korrigan Sciences Limited (Maidenhead, Berkshire, UK) for providing the MATLAB functions for analysis of the NMR data. This study was funded by the Swiss National Science Foundation (WW); the 7th EU program TORNADO (WW, AN); the Bonizzi-Theler-Stiftung (WW); the Région Midi-Pyrénées (HG, Mathusalem Program; WW, Chaire d’Excellence Pierre de Fermat); the Etat de Vaud (WW); and the Start-Up Grant (WW) from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore. VK and GSO thank the BII/A-STAR for financial support of this study. Open access funding was provided by University of Vienna.
The authors declare no competing interests.
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Duszka, K., Ellero-Simatos, S., Ow, G.S. et al. Complementary intestinal mucosa and microbiota responses to caloric restriction. Sci Rep 8, 11338 (2018). https://doi.org/10.1038/s41598-018-29815-7
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