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Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes

Nature Biotechnology volume 38, pages 577–578 (2020)Cite this article

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An Author Correction to this article was published on 09 March 2020

The Original Article was published on 24 February 2020

This article has been updated

replying to Horlbeck, M.A. et al. Nature Biotechnology https://doi.org/10.1038/s41587-020-0428-0 (2020)

In this issue, Weissman and colleagues1 argue that the results in our recent paper2 on CRISPR/Cas9-based screening for functional long noncoding RNAs (lncRNAs) could have been affected by (1) copy-number effects on single-guide RNA (sgRNA) dropout and (2) lncRNA genes that overlap with protein-coding genes. We have carefully reanalyzed our screening results to evaluate the validity of their concerns.

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Data availability

All data used are from our original paper2.

Code availability

Source code for the computational analysis described in this paper is available in Supplementary Code.

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References

  1. Horlbeck, M. A. et al. Fitness effects induced by CRISPR/Cas9-targeting of long noncoding RNA gene loci. Nat. Biotechnol. https://doi.org/10.1038/s41587-020-0428-0 (2020).

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Author information

Authors and Affiliations

  1. Biomedical Pioneering Innovation Center, Beijing Advanced Innovation Center for Genomics, Peking-Tsinghua Center for Life Sciences, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China

    Ying Liu, Zhiheng Liu, Zhongzheng Cao & Wensheng Wei

  2. Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China

    Zhiheng Liu & Zhongzheng Cao

Authors
  1. Ying Liu
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  2. Zhiheng Liu
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  3. Zhongzheng Cao
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  4. Wensheng Wei
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Contributions

Z.L. performed the bioinformatics analysis. Y.L. and Z.C. integrated all the results. Y.L., Z.C. and W.W. wrote the manuscript with the help of Z.L.

Corresponding author

Correspondence to Wensheng Wei.

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Competing interests

W.W. is a founder of and scientific advisor to EdiGene.

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Supplementary information

Analysis of lncRNAs

Computer code

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Liu, Y., Liu, Z., Cao, Z. et al. Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes. Nat Biotechnol 38, 577–578 (2020). https://doi.org/10.1038/s41587-020-0431-5

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