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The hyphal-specific toxin candidalysin promotes fungal gut commensalism

Nature volume 627, pages 620–627 (2024)Cite this article

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Abstract

The fungus Candida albicans frequently colonizes the human gastrointestinal tract, from which it can disseminate to cause systemic disease. This polymorphic species can transition between growing as single-celled yeast and as multicellular hyphae to adapt to its environment. The current dogma of C. albicans commensalism is that the yeast form is optimal for gut colonization, whereas hyphal cells are detrimental to colonization but critical for virulence1,2,3. Here, we reveal that this paradigm does not apply to multi-kingdom communities in which a complex interplay between fungal morphology and bacteria dictates C. albicans fitness. Thus, whereas yeast-locked cells outcompete wild-type cells when gut bacteria are absent or depleted by antibiotics, hyphae-competent wild-type cells outcompete yeast-locked cells in hosts with replete bacterial populations. This increased fitness of wild-type cells involves the production of hyphal-specific factors including the toxin candidalysin4,5, which promotes the establishment of colonization. At later time points, adaptive immunity is engaged, and intestinal immunoglobulin A preferentially selects against hyphal cells1,6. Hyphal morphotypes are thus under both positive and negative selective pressures in the gut. Our study further shows that candidalysin has a direct inhibitory effect on bacterial species, including limiting their metabolic output. We therefore propose that C. albicans has evolved hyphal-specific factors, including candidalysin, to better compete with bacterial species in the intestinal niche.

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Fig. 1: The colonization fitness of C. albicans WT and yeast-locked cells is dependent on the mouse model.
Fig. 2: Colonization fitness of WT and yeast-locked C. albicans cells in hosts harbouring different bacterial populations.
Fig. 3: Candidalysin promotes C. albicans colonization in mice harbouring intact (antibiotic-naive) bacterial microbiomes.
Fig. 4: Candidalysin peptide exhibits direct anti-bacterial activity.

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Data availability

The 16S sequencing data are publicly available at the NCBI Sequence Read Archive (SRA) and can be accessed under BioProject PRJNA1008281. Microarray data for analysis of C. albicans expression are available at ArrayExpress under accession MTAB-13349. Source data are provided with this paper.

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Acknowledgements

This work was supported by NIH grants AI166869, AI141893, AI081704 and AI168222 to R.J.B. Y.-H.C. was supported by the Charles H. Revson Senior Fellowship in Biomedical Science. B.H. and T.B.S. were supported by the German Research Foundation (Deutsche Forschungsgemeinschaft (DFG)) within the Cluster of Excellence ‘Balance of the Microverse’, under Germany’s Excellence Strategy, EXC 2051, project ID 390713860. B.H. and S.A. were further supported by the DFG, project HU 528/20-1. J.C.P was supported by AI175081, P.B. was supported by DK125382 and S.P. was supported by a Graduate Research Fellowship from the NSF under award number 1644760. I.V.E. was supported by the Institut Pasteur and is a CIFAR Azrieli Global Scholar in the CIFAR Program Fungal Kingdom: Threats & Opportunities. We thank E. Pamer, I. Jacobsen and T. Hohl for sharing strains and the National Gnotobiotic Rodent Resource Center (NIH grants P40 OD010995 and P30 DK034987) for support.

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Author notes

  1. These authors contributed equally: Shen-Huan Liang, Shabnam Sircaik

Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology, Brown University, Providence, RI, USA

    Shen-Huan Liang, Shabnam Sircaik, Joseph Dainis, Pallavi Kakade, Swathi Penumutchu, Liam D. McDonough, Corey Frazer, Shipra Vaishnava, Peter Belenky & Richard J. Bennett

  2. Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA

    Ying-Han Chen & Ken Cadwell

  3. Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology–Hans Knoell Institute (HKI), Jena, Germany

    Tim B. Schille, Stefanie Allert, Osama Elshafee, Maria Hänel, Selene Mogavero & Bernhard Hube

  4. Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Jena, Germany

    Tim B. Schille & Bernhard Hube

  5. Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA

    J. Christian Perez

  6. Institute of Microbiology, Friedrich Schiller University Jena, Jena, Germany

    Bernhard Hube

  7. Institut Pasteur, Université Paris Cité, Fungal Heterogeneity Group, Paris, France

    Iuliana V. Ene

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Contributions

R.J.B. conceived the majority of the experiments and wrote the initial manuscript draft, with input from S.-H.L. and S.S. S.-H.L. and S.S. performed the majority of the experiments. P.K. performed the microscopic analysis of yeast and hyphal cells. L.D.M. constructed strains and analysed in vivo phenotypes. J.D. performed analyses of C. albicans strains with individual bacterial strains in mice. C.F. assisted with design and construction of C. albicans mutant strains. S.V. provided gnotobiotic mice and advised on the project. J.C.P., K.C. and Y.-H.C. performed consortia experiments. S.P. and P.B. analysed 16S data and generated related figures. T.B.S., S.A., M.H., S.M. and O.E. performed analyses of C. albicans with candidalysin. O.E. performed in vitro C. albicans competition experiments. S.A. and S.M. performed transcriptional profiling of the ece1 mutant. B.H. conceived the in vitro experiments, provided the C. albicans ece1 mutant strains and advised on the project. I.V.E. performed in vivo experiments and advised on the project.

Corresponding authors

Correspondence to Bernhard Hube or Richard J. Bennett.

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Extended data figures and tables

Extended Data Fig. 1 Comparison of the fitness of WT and yeast-locked efg1Δ/Δ cells from different C. albicans strain backgrounds.

a, Experiments were performed as described in Fig. 1. Mouse GI organs (St; stomach, Si; small intestine, Ce; cecum, Co; colon) were homogenized to identify WT and efg1Δ/Δ cells 14 days post-C. albicans inoculation. WT (CAY2698) versus efg1Δ/Δ (CAY11750) competitions in SD + P/S (P/S; penicillin/streptomycin) model (a), PD + P/S model (b) and PD model (c). dpi; days post-inoculation. d, Schematic of competition between WT and yeast-locked efg1Δ/Δ cells. Mice fed the SD without antibiotics were gavaged with a 1:1 mixture of WT and efg1Δ/Δ cells and C. albicans colonies were examined in fecal pellets at the indicated time points. e,f, Competition outcomes (e) and fecal CFUs (f) of WT versus efg1Δ/Δ in 529L (CAY5016 versus CAY11482) and CHN1 (CAY11170 versus CAY11184). Experiments were performed in BALB/c and C57BL/6 J mice as indicated. Each data point represents an individual mouse, data are mean ± s.e.m in a, b, and f. n = 3 for competitions in BALB/c mice and n = 4 for competitions in C57BL/6 J mice. A paired t-test (two-tailed) was used in e and a Mann-Whitney (two-tailed) test in f.

Source Data

Extended Data Fig. 2 Comparison of the fitness of WT and flo8Δ/Δ cells in different colonization models.

a, Microscopic images of cells in the colon of BALB/c SD + P/S mice infected with WT (CAY2698) or flo8Δ/Δ (CAY9796) cells at 7 days post-infection. b, Quantitative analysis of yeast and filamentous morphotypes. n = 2. c, Schematic of competition between WT and flo8Δ/Δ cells. n = 2. Each data point represents an individual mouse. A 1:1 mix of WT and flo8Δ/Δ cells were inoculated by oral gavage and fecal samples were collected at the indicated time points. d-f, Results for SC5314 WT (CAY2698) versus flo8Δ/Δ (CAY9796) cells in GF BALB/c mice (d), conventional PD-fed BALB/c mice treated with antibiotics (P/S; penicillin/streptomycin) (e), and conventional PD-fed BALB/c mice (without antibiotics) (f). dpi; days post-inoculation. n = 3 in e and f. g-i, Competition of WT versus flo8Δ/Δ cells in BALB/c mice fed a SD (without antibiotics). Experiments were performed using SC5314 (CAY2698 vs. CAY9796) (g), 529 L (CAY11168 vs. CAY11180) (h), or CHN1 (CAY11170 vs. CAY11186) (i) strain backgrounds. n = 3. Each data point represents an individual mouse. Data are mean ± s.e.m in b. Significance was determined by a Mann-Whitney (two-tailed) test in b and a paired two-tailed t-test in d-i.

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Extended Data Fig. 3 Analysis of microbiome composition upon changes in diet, antibiotic treatment, and C. albicans colonization.

a, Schematic of colonization experiments. BALB/c mice were fed the SD or PD, with or without antibiotics (P/S; penicillin/streptomycin). These groups were compared with and without colonization with C. albicans SC5314 cells. b, C. albicans CFUs in fecal pellets collected at the indicated time points. LOD, limit of detection. n = 8. c,d, Total bacterial levels in fecal pellets collected at the indicated time points by quantitative PCR in SD- or PD-fed mice with antibiotics (c) and without antibiotics (d). n = 8. e, Bubble plot depicting the amount of variation in gut microbial composition determined by Permutational Multivariate Analysis of Variance (PERMANOVA) analysis using the adonis function and Bray-Curtis distances of beta diversity. Effect size refers to the magnitude of the differences or dissimilarities between groups. f, Shannon diversity of control mice on SD or PD (n = 8). g-m, Distribution of bacterial phyla in different models as determined by MaAsLin2. PD vs. SD (g), SD vs. SD + P/S (h), PD vs. PD + P/S (i), SD +/- C. albicans (j), PD +/- C. albicans (k), PD + P/S +/- C. albicans (l) and SD + P/S +/- C. albicans (m). Coefficient with standard error shown on x-axis. adj p-val cutoff 0.05. n, Relative abundance of Actinobacteria across different diet fed mice (+/− Candida) on Day 21. n = 8. Each data point represents an individual mouse. Data are mean ± s.e.m in b-m. A Mann-Whitney test was used in b,f,and n.

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Extended Data Fig. 4 Linear discriminant analysis Effect Size (LEfSe) analysis to compare the alterations in gut bacterial populations in mice on different diets.

Linear discriminant analysis (LDA) effect size for significant taxa in the microbiome of mice fed the SD vs. PD are plotted onto a cladogram for Day 0 (a), Day 7 (b), Day 14 (c), Day 21 (d). and for mice fed the PD (+ /− C. albicans) for Day 0 (e), Day 7 (f), Day 14 (g) and Day 21 (h). Analysis was performed on mice as shown in Extended Data Fig. 3 (n = 8 per group). Differentially abundant species with LDA score >2 are shown in nodes represented with red (PD) and green (SD) and non-significant species are represented with yellow. A Kruskal-Wallis test was used to compare features between diets (p < 0.05) and the Pairwise Wilcoxon test was used to compare between taxa (p < 0.05).

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Extended Data Fig. 5 Analysis of gut bacterial populations in different murine models.

16S rRNA sequencing data was used to determine the relative abundance of bacterial phyla in BALB/c colonization experiments using mice fed the SD or PD, and supplemented or not supplemented with antibiotics (P/S; penicillin/streptomycin). Each group of mice were also compared +/− inoculation with C. albicans SC5314 cells, as shown in Extended Data Fig. 3. a,b, Shannon diversity for bacterial populations from fecal pellets for the shown experiments. n = 8. c, Relative abundance of bacteria shown for fecal pellets for days 0, 10, 14, and 21, and for the small intestine at day 21. n = 8. Data are mean ± s.e.m in a-c.

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Extended Data Fig. 6 C. albicans WT cells outcompete efg1Δ/Δ cells in gnotobiotic mice harboring different bacterial populations.

a, Schematic of competition between WT and yeast-locked efg1Δ/Δ cells in gnotobiotic colonization models. Mice were gavaged with a 1:1 mixture of WT SC5314 (CAY2698) and efg1Δ/Δ (CAY11750) cells. b-d, C. albicans cells were tested in GF BALB/c mice (b), GF NMRI mice (c), or GF C57BL/6 mice colonized with E. coli prior to inoculation with C. albicans (d). n = 4 in b, 8 in c, and 5 in d. e, C. albicans CFUs in fecal pellets collected at the indicated time points in GF NMRI mice (e) and GF C57BL/6 mice (f). n = 8 in e, n = 3–5 in f. LOD, limit of detection. g, WT v. efg1Δ/Δ competition in Amp-treated mice gavaged with heat-killed AmpR E. coli. n = 4. h,i, Competition between CHN1 WT (CAY11170) and efg1∆/∆ (CAY11184) cells in WT mice (h) or Rag1-/- mice (i) on the SD (no antibiotics). n = 4. j, C. albicans CFU levels in the fecal pellets from WT and Rag1-/- mice. n = 4. Each data point represents an individual mouse. Data are mean ± s.e.m in j. A paired t-test (two-tailed) was used in b-d and g-i, and a Mann-Whitney (two-tailed) test in e,f and j.

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Extended Data Fig. 7 Examining the role of ALS3 and SOD5 in GI colonization fitness.

a, Schematic of competition between WT C. albicans and sod5Δ/Δ or als3Δ/Δ cells (SC5314 background). b-e, BALB/c mice fed a PD without antibiotics or a SD with antibiotics (P/S; penicillin/streptomycin). Experiments used WT (CAY8785) and sod5Δ/Δ (CAY14738) in b and c. n = 4. WT (CAY8785) and als3Δ/Δ (CAY14696) in d and e. n = 8. Each data point represents an individual mouse. A paired t-test (two-tailed) was used for statistical significance.

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Extended Data Fig. 8 Colonization fitness of WT and ece1Δ/Δ mutants.

Experiments were performed as described in Fig. 3 Mouse GI organs (St; stomach, Si; small intestine, Duo; duodenum, Je; jejunum, Ile; ileum, Ce; cecum, Co; colon) were homogenized to identify the ratio of WT and ece1Δ/Δ cells 14 days post C. albicans infection. b, Competition between CAY11533 (WT) versus CAY11507 (ece1Δ/Δ) cells in BALB/c fed a PD. n = 5. c, Competition between WT (CAY11168) and ece1Δ/Δ cells (CAY12441) in the 529L background BALB/c mice fed a PD. n = 4. d, Competition between WT (CAY11170) and ece1Δ/Δ cells (CAY12446) in the CHN1 background in SD-fed BALB/c mice. n = 4. e-g, Competition between CAY12202 (WT) versus CAY8578 (ece1Δ/Δ) BALB/c mice fed a SD plus antibiotics (P/S; penicillin/streptomycin) (e), GF C57BL/6 mice (f), and GF C57BL/6 mice colonized with E. coli prior to C. albicans inoculation (g). n = 4 in e, and 5 in f and g. Competition outcomes of WT (CAY12202) versus ece1Δ/Δ+ECE1ΔpIII (CAY8580) in GF C57BL/6 mice (h) and in BALB/c mice fed a PD (i). n = 4 in h and i. Each data point represents an individual mouse. Data are mean ± s.e.m in c–e, h, i. A paired t-test (two-tailed) was used to determine the significance between two populations (b-h).

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Extended Data Fig. 9 Comparison of C. albicans WT and ece1Δ/Δ cells in vitro.

a, Heat map of transcriptome analysis representing the fold change (FC) in expression of fungal genes when strains were grown under hyphal-inducing conditions (3 h, 37 °C, 5% CO2). b-h, Competitive fitness of SC5314 WT and ece1Δ/Δ cells in RPMI (37 °C, 5% CO2) (b), acidic pH (pH 4.7) (c), high salt (1 M NaCl) (d), hypoxia (1% O2) (e), oxidative stress (2 mM H2O2) (f), in the presence of 2% acetate (g), and during incubation with TR146 epithelial cells (h). Competitive index was calculated using the formula: log2 [(MUTcompetition/WTcompetition)/ (MUTsingle/WTsingle)]. Fitness of the ece1Δ/Δ relative to the WT is represented as log2 competitive index. Data are representative of three biological replicates (n = 3). Graphs show the mean ± sem in b - h. Statistical analysis was performed using 1-way ANOVA with Bonferroni post hoc test to detect significance.

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Liang, SH., Sircaik, S., Dainis, J. et al. The hyphal-specific toxin candidalysin promotes fungal gut commensalism. Nature 627, 620–627 (2024). https://doi.org/10.1038/s41586-024-07142-4

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