Conserved regions of the circular DNA sequence of the M13mp18 bacteriophage, which is used as a scaffold for DNA origami construction, are targeted with specific hybridization-chain-reaction probes. The probes enable sensitive detection of DNA origami nanostructures in cells, organoids and tissues to assess their biodistribution and stability.
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Patino, T. Imaging DNA origami by fluorescence in situ hybridization. Nat. Nanotechnol. 19, 1–2 (2024). https://doi.org/10.1038/s41565-023-01556-3
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DOI: https://doi.org/10.1038/s41565-023-01556-3