Abstract
Lactic acid bacteria (LAB) are fundamental in the production of fermented foods and several strains are regarded as probiotics. Large quantities of live LAB are consumed within fermented foods, but it is not yet known to what extent the LAB we ingest become members of the gut microbiome. By analysis of 9445 metagenomes from human samples, we demonstrate that the prevalence and abundance of LAB species in stool samples is generally low and linked to age, lifestyle, and geography, with Streptococcus thermophilus and Lactococcus lactis being most prevalent. Moreover, we identify genome-based differences between food and gut microbes by considering 666 metagenome-assembled genomes (MAGs) newly reconstructed from fermented food microbiomes along with 154,723 human MAGs and 193,078 reference genomes. Our large-scale genome-wide analysis demonstrates that closely related LAB strains occur in both food and gut environments and provides unprecedented evidence that fermented foods can be indeed regarded as a possible source of LAB for the gut microbiome.
Similar content being viewed by others
Introduction
For several decades, lactic acid bacteria (LAB) have been among the most extensively studied microorganisms. LAB have a fundamental role in different biological processes and ecosystems, especially with respect to fermented foods. The microbiology of fermentations has been extensively studied for over a century and the ability to transform raw materials into edible products with defined characteristics dates back to thousands of years as a strategy of food preservation1,2. Industrial fermentations are based on selected cultures that are used as starters or adjuncts to guarantee specific metabolic activities along with quality, reproducibility, and safety. On the other hand, artisanal processes do not usually involve defined starter cultures and the LAB available in the raw materials, or sourced from a previous manufacturer, lead the fermentation. Food-associated LAB have been studied mainly from the perspective of their fermentation performances and phenotypic properties, and knowledge on such properties has recently increased, thanks to intense genome sequencing of LAB strains3,4.
Apart from their contributions to food quality and safety, LAB have attracted considerable interest due to their potentialities to add functional properties to certain foods or as supplements. Functional foods are designed to deliver additional benefits over their basic nutritional values and contribute to human health5. In this regard, several LAB species and strains have been recognized as probiotics, i.e., “live microorganisms that confer a health benefit on the host when administered in adequate amounts6.” Importantly, many LAB species also enjoy a generally recognized as safe status.
Despite the extensive literature focusing on characterizing LAB in food, it is still not fully understood how they interact with the human gut microbiome5. Ingested LAB need to first survive the physical and chemical barriers of the gut, before competing with hundreds of different species, and finally being able to exert their beneficial effects. Indeed, LAB are regarded as components of the transient gut microbial community, coming from the external environment and with food representing the main source, which interacts daily with the longer term members of the gut microbiome7. Despite this general view, it is still not known to what extent components of the food microbiome are actively transferred to become part of the gut microbiome and what role they play in this complex environment. Depending on the specific food, technology of production, and fermentation process, fermented foods can harbour several LAB species and strains, and are natural sources of live microorganisms that are consumed daily across all human populations, and that can potentially interact with the gut microbiome. Despite this, the degree to which LAB species and strains not explicitly regarded as probiotics can be transferred to the gut has been largely underexplored. In addition, no studies have been conducted to assess the distribution of LAB in the global population, a gap that may be bridged by taking advantage of the growing availability of high-throughput sequencing data.
In this study, we perform a large-scale genome-wide analysis of publicly available and newly sequenced food and human metagenomes to investigate the prevalence and diversity of LAB species with a view to identifying links between gut and food microbiomes. We find that LAB species occur with variable prevalence and generally low abundance in the human gut. Such prevalence is affected by age and lifestyle. LAB species identified in food only partially match those in the gut. Comparative genomics suggest an overall food origin for the gut strains.
Results
Large-scale meta-analysis on food and human microbiomes
We performed a large-scale meta-analysis on microbiomes from food sources and human body sites to investigate the prevalence and diversity of LAB species in the human microbiome and their overlap with species and strains found in food. To achieve this goal, we considered 303 food metagenomes (152 publicly available and 151 obtained in this study) (11 datasets; Table 1 and Supplementary Data 1) that we curated in this study, which corresponded to different types of fermented foods and beverages8,9,10,11,12,13,14. In addition, we considered 9445 human metagenomes from 47 public datasets spanning multiple body sites (84% from the gut), age categories, countries, and lifestyles, which we retrieved from recent meta-analyses15,16.
Variable prevalence of LAB in the human gut
We considered reference-based taxonomic profiles17 of all 9445 human metagenomes15,16 (see “Methods”) and focused specifically on LAB species in this study (Supplementary Data 2). We detected 152 species belonging to the Lactobacillales order occurring in at least one of the metagenomes with a relative abundance >0.01%. Among them, we identified 70 species belonging to the LAB group and restricted the following analysis to the 30 of them having a prevalence >0.1% in the human gut (see “Methods”). These represented mainly species (spanning Lactobacillus, Lactococcus, Leuconostoc, Streptococcus, and Weissella genera) of potential food origin, including bacteria occurring in probiotic supplements, in addition to typically non-food origin species such as Lactobacillus mucosae, Lactobacillus ruminis, and Lactobacillus salivarius (Fig. 1). The two most prevalent species in the gut were Streptococcus thermophilus (prevalence 31.2%, i.e., present at >0.01% relative abundance in 31.2% of the gut metagenomes) and Lactococcus lactis (16.3%), both commonly found in dairy products (Fig. 1, Supplementary Fig. 1, and Supplementary Data 3). Multiple Lactobacillus species of predominantly food origin were detected at lower prevalence (3–5%) and comprised Lactobacillus casei/paracasei, Lactobacillus delbrueckii, Lactobacillus fermentum, and Lactobacillus rhamnosus). Non-food origin bacteria were also identified at remarkable levels such as Lb. ruminis (11.0%), Lb. salivarius (4.7%), and Lb. mucosae (4.0%). Although prevalence was variable, average relative abundance (computed on positive samples only) of single species was generally rather low (<2%), including the case of the two most prevalent species S. thermophilus (0.6%) and Lc. lactis (0.4%). Exceptions (rel. ab. >2%) were verified for Lactobacillus amylovorus, Lactobacillus brevis, and Lactobacillus buchneri, which however rarely occurred (prev. <1%).
Strong age-related patterns were verified for some of the species prevalent in gut samples (N = 7907) (Fig. 1, Supplementary Fig. 2, and Supplementary Data 4). S. thermophilus increased in prevalence from newborns (8.4%) to adults (33.7%, p < 1e − 40), with comparable average abundance. This may reflect the increase in consumption of yoghurts and other dairy products that can be sources of S. thermophilus18. A similar pattern was observed for Lb. delbrueckii (p < 1e − 10) and the non-food origin species Lb. mucosae (p < 1e − 10), Lb. ruminis (p < 1e − 20), and Lb. salivarius (p < 1e − 10), which suggests their gut colonization later in age. Also, Lc. lactis had higher prevalence in adults (15.8%) than newborns (8.6%, p < 1e − 6), with its detection in only one infant cohort originating from Estonia, Finland, and Russia19. Other lactobacilli were more prevalent and abundant in newborns such as Lb. casei/paracasei (p < 1e − 20 with respect to adults), Lactobacillus gasseri (p < 1e − 7), Lactobacillus plantarum (p < 1e − 4), and Lb. rhamnosus (p < 1e − 70). These species have also been detected in human breast milk20, suggesting their possible transmission from mother to infant through breastfeeding, as previously reported for Lb. plantarum21. Notably, these species were not found to be vertically transmitted from other mother’s body sites22.
Overall, we found that LAB are a subdominant component of the gut microbiome, although several species exhibited non-negligible contributions. More specifically, we identified 21 LAB occurring with prevalence >1% and 18 with relative abundance >0.5% when detected in the gut. It is reasonable to hypothesize that those species may be short- or long-term colonizers of the human microbiome.
Occurrence and abundance of LAB is linked to lifestyle
We then stratified the gut metagenomes in terms of host lifestyles (Fig. 1, Supplementary Fig. 3, and Supplementary Data 4), which revealed variations in prevalence and abundance between westernized and non-westernized populations for multiple species. Higher prevalence in westernized populations was observed for six lactobacilli, mostly of food origin, such as Lactobacillus acidophilus (p < 1e − 6), Lb. casei/paracasei (p < 1e − 4), Lb. delbrueckii (p < 0.01), Lb. gasseri (p < 1e − 6), Lb. rhamnosus (p < 1e − 9), and Lactobacillus sakei (p < 1e − 3). By contrast, Lb. mucosae (p < 1e − 8) and Lb. ruminis (p < 1e − 100) that do not occur in food were more prevalent in the non-westernized cohorts. Despite different patterns in terms of prevalence, all lactobacilli were on average more abundant in the westernized populations. Among the other genera, S. thermophilus was highly prevalent in the westernized cohorts (p < 1e − 50). Higher prevalence in the non-westernized group was observed for Lactococcus garvieae (p < 1 − e30) in addition to multiple heterofermentative species such as Leuconostoc citreum (p < 1e − 70), Leuconostoc lactis (p < 1e − 60), Weissella cibaria (p < 1e − 10), and Weissella confusa (p < 1e − 100), which is consistent with their widespread prevalence in raw vegetables23 that are likely consumed in such populations. In fact, non-western populations usually have hunter–gatherer diet and lifestyle, which is recognized to be characterized by high consumption of tubers, drupes, roots, and fruits24,25. Indeed, it was also reported that the!Kung and the Hadza, two non-Western African populations, still obtain 60–80% and 50–65% of their diet from plant foods, respectively26.
We further grouped metagenomes by host country of origin (see “Methods”) and identified more subtle geographical variations (Fig. 1 and Supplementary Fig. 4). Overall, food-associated lactobacilli were most prevalent and abundant in Europe, were less so in Asia and North America, and were almost absent in China (kept distinct from the other Asian countries due to its large sample size) and in the non-westernized populations. The higher prevalence in European cohorts was significant (p < 0.05) for Lb. casei/paracasei (8.0%), Lb. delbrueckii (6.6%, with a similar value in Asia), and Lb. rhamnosus (7.1%). Exceptions were Lb. gasseri, having comparable prevalence in continents including westernized cohorts, and Lb. fermentum, more prevalent in North America, South America, and China, with the latter observation being consistent with its widespread occurrence in Chinese fermented foods27. Non-food lactobacilli were not prevalent in Europe. Lb. mucosae exhibited high prevalence (>10%) in Africa, China, and South America, with comparable abundance across the globe. A similar trend was verified for Lb. ruminis, although with higher prevalence in non-westernized cohorts, whereas the presence of Lb. salivarius was distinctive for the Chinese population (p < 0.01). Among the other genera, Lc. lactis exhibited high prevalence across the entire globe (ranging from 11.5% in Africa to 44.4% in South America) with the sole exception of China (1.7%). S. thermophilus reached high prevalence in Asia (41.5%), Europe (39.6%), and North America (28.1%), but was much less prevalent in the Chinese (5.6%) and non-westernized (<3%) cohorts.
LAB species from food only partially match those in the gut
We established genome level links between the microorganisms populating the human microbiome and those found in food by integrating the genomes reconstructed from a set of 9445 human metagenomes with those from the set of 303 food metagenomes that we generated, collected, and curated in this work (Table 1 and Supplementary Data 1). More specifically, we considered 303 metagenomic samples spanning 11 datasets and coming from different types of cheese (N = 191), multiple fermented foods (N = 58), nunu (N = 20), milk kefir (N = 18), and yoghurt and dietary supplements (N = 16). We applied a validated16,28 computational pipeline that combined single-metagenome assembly, contig binning, and genome quality control to reconstruct de novo metagenome-assembled genomes (MAGs) from the set of food metagenomes (see “Methods”). We generated a total of 666 food MAGs (completeness > 50% and contamination < 5%) of sufficient quality according to previous recommendations29. These MAGs from food were integrated with the set of 154,723 MAGs that we retrieved from the 9445 human metagenomes using the same assembly-based pipeline16 and with the set of 193,078 reference genomes (available in GenBank as of March 2019). This resulted in a total of 348,467 genomes that were clustered at 5% genetic distance based on whole-genome nucleotide similarity estimation and recapitulated in species-level genome bins (SGBs, i.e., clusters of genomes spanning 5% genetic diversity, see “Methods”). The 666 food MAGs were grouped into 171 SGBs (Supplementary Data 5 and 6), which we discuss below on the basis of their occurrence in food samples and human gut (Fig. 2a, b).
Most of the food MAGs (349, 52.4%) belonged to SGBs also found in the human gut, with 265 of them associated with twenty of the thirty LAB species discussed previously (Fig. 2a top panel and Supplementary Fig. 5). The species most reconstructed from food sources was Lc. lactis (N = 90 MAGs), with 86 MAGs extracted from cheese. Sixty MAGs were associated with S. thermophilus, the majority of them was reconstructed from cheese and yoghurt, and five additional genomes were extracted from different fermented foods such as wagashi, beetroot kvass, ryazhenka, ruž’a, and labne. A consistent number of MAGs was also retrieved from Lactobacillus helveticus (33 MAGs from cheese), Lactobacillus curvatus (14 MAGs from cheese and 1 from sauerkraut), Lb. delbrueckii (11 MAGs from cheese or yoghurt in addition to single genomes from dietary supplement and tofu), Leuconostoc mesenteroides (5 MAGs from nunu and single genomes from bread kvass, ginger beer, milk kefir, beetroot kvass, ruž’a, and cheese), and Lb. casei/paracasei (4 MAGs from cheese, 2 MAGs from dietary supplements, and 2 MAGs from water kefir). We also extracted four MAGs of Lb. mucosae, a typical non-food microorganism that is usually found in the intestine of pigs or other animals30, and which we instead reconstructed from different fermented foods such as kimchi, kombucha vinegar, agousha, and sauerkraut.
We identified 17 additional non-LAB SGBs having MAGs from both food and human metagenomes, for a total of 84 food MAGs (12.6%; Fig. 2a bottom panel) and spanning three phyla (namely Actinobacteria, Firmicutes, and Proteobacteria). Some of these may be microbial contaminants in the food chain that can arise from different sources including animal, feed, and soil31,32. The SGB with the most MAGs (N = 16) was that containing Streptococcus equinus and Streptococcus infantarius genomes, two species usually found in the rumen33 but occasional pathogens for humans34, and which we found in African fermented foods13.
The majority of the food SGBs (134 out of 171), accounting for 317 MAGs (47.6%), did not exhibit an overlap with human MAGs, likely representing species unable to reach the colon or characterized by low prevalence and abundance in the human gut (Fig. 2b). Among them, 71 SGBs (53.0%; comprising 225 MAGs) contained at least one reference genome (kSGBs; Fig. 2b left panel). The most prevalent food-specific species was Brevibacterium linens (24 MAGs), which was reconstructed from multiple cheese types (i.e., surface ripened8, smear ripened14, hard, and tomme). Food-specific SGBs also included Staphylococcus saprophyticus (13 MAGs), Glutamicibacter arilaitensis (12 MAGs), and 58 MAGs from 21 LAB species spanning 6 families, the most prevalent being Lc. lactis subsp. cremoris. This set of MAGs and reference genomes showed a >5% genetic distance from Lc. lactis subsp. lactis genomes35, which we kept as a separate SGB (ID 7985) and found to be prevalent in both food and human metagenomes, in contrast to Lc. lactis subsp. cremoris, which was only detected in food metagenomes. Similarly, Lactococcus raffinolactis was divided into two SGBs, with human and food MAGs grouped in the SGBs 7989 and 7991, respectively.
Out of the 134 SGBs not overlapping with human MAGs, 63 SGBs (47%; comprising 92 MAGs) consisted of MAGs reconstructed in this study from food metagenomes without any reference genomes. These represented new species currently not represented in public repositories (Fig. 2b right panel), of which only 12 were assigned to known genera, and which should be targeted for cultivation-based analysis.
The set of genomes reconstructed and the SGBs identified in this study and that we made publicly available (see “Methods”) facilitated a more in-depth comparative genomics analysis.
Comparative genomics suggests a food origin for the gut strains
Within the available set of MAGs and reference genomes, we performed strain-level comparative genomic analysis for the set of 348,467 genomes previously described and comprising 193,078 reference genomes, 154,723 human MAGs, and 666 food MAGs. The 2859 genomes (including 1042 MAGs) associated with the thirty LAB species of interest were kept for comparative genomics purposes. To inform the comparative analysis, we retrieved and manually curated the source types for all genomes (see “Methods”) and grouped MAGs and reference genomes in three categories: human, food, and other. Genomes for which this information was missing were labelled as NA (7.9% of genomes; Fig. 2c, Supplementary Fig. 6, and Supplementary Data 7).
Overall, two-thirds of the reference genomes came from food (43.8%) and human sources (21.0%). The group of genomes from strains not isolated from foods or humans (22.8%) comprised 67 genomes from probiotics and dietary supplements in addition to 347 genomes mainly coming from animal sources. The proportions of species assigned to the different source types was quite variable across species, with a general under-representation of human genomes corresponding to LAB that were prevalent in non-westernized cohorts (Fig. 2c and Supplementary Fig. 6). This reflected the overall scarce availability of genome from isolates for a substantial fraction of the non-pathogenic, commensal members of the human microbiome as recently highlighted16,36,37. Reference genomes from human samples were surprisingly almost absent in the case of prevalent species such as Lc. lactis (with only one reference genome from the vagina and one MAG from the gut) and S. thermophilus (with only one MAG from the gut). The absence of good reference genomes in public repositories prevented the comparison of food and human strains until now, which we aimed to overcome in the present study through an extensive comparative genomics analysis.
S. thermophilus was the species of LAB most frequently reconstructed from metagenomes (243 human and 60 food MAGs; Fig. 3a), an observation consistent with its high prevalence from mapping-based taxonomic profiling (Fig. 1). Comparative genomics, also including 44 reference genomes, did not highlight food-specific or gut-specific sub-clades, suggesting that food can be regarded as the main source of this species in the human microbiome. S. thermophilus also appeared to be a quite genetically diverse species both in food and human sources with MAGs reconstructed from Asian gut metagenomes enriched in a specific clade (Clade A, Fig. 3a, p < 1e − 10). Lb. delbrueckii was not prevalent in the gut, and the only two subspecies found in human samples were subsp. lactis and subsp. bulgaricus (Fig. 4a). Human MAGs of both subspecies clustered together with food MAGs and isolates, again indicating food as the most likely source of this species in the gut. On the other hand, subsp. delbrueckii, subsp. sunkii, and subsp. jakobsenii were found in food, but never reconstructed from the gut. Although Lb. rhamnosus was the LAB species for which the greatest number of genomes corresponding to human isolates (N = 105) was available, we collected only 32 human MAGs, which is in agreement with its low prevalence and abundance in the gut (Fig. 4b). We identified a specific cluster including 17% of the Lb. rhamnosus human genomes that included the reference genome associated with the Lb. rhamnosus strain GG (LGG), which may be due to recent consumption of commercial products due to its wide use in probiotic supplements38.
The highest number of food MAGs was obtained for Lc. lactis (N = 90, Fig. 3b). We refer here to subsp. lactis, whereas subsp. cremoris was associated with 12 food MAGs but never reconstructed from human metagenomes. Lc. lactis subsp. lactis formed two distinct clusters including both food and human genomes. The first cluster included 63% of the genomes, exhibited an overall low diversity (<0.8% genetic distance between closest genome pairs), and included all the food genomes related to cheese and dairy fermentation. The second cluster was more diverse, dominated by environmental and raw vegetable products, and included the only MAG from human skin and the three gut MAGs from non-westernized cohorts. An additional cluster containing two genomes from nunu13 was never found in humans and exhibited a >3% genetic diversity from all other genomes. Such results highlighted the overall importance of conducting strain-level analysis on the food-gut axis, depicted here by the identification of two main clusters in the human gut associated with different food sources (i.e., one from cheese and dairy fermentation, and the other one from environmental and raw vegetables products). Strains of these clusters are likely characterized by differences in functional traits and potential interaction with the host that deserve to be investigated in future studies.
The SGB 7142 (N = 216, Fig. 4c), labelled Lb. casei/paracasei, included reference genomes identified as both Lb. casei and Lb. paracasei, which, as recently highlighted, can be used interchangeably39. Within the combined species, we detected two main clusters, both of which occurred in food and human samples. The major cluster contained 86% of the available genomes, including all the dietary supplement strains and the majority (86%) of the human MAGs. Consistent with its low abundance (Fig. 1), only seven reference genomes and a single MAG were reconstructed from human samples for Lb. helveticus (Fig. 4d). We identified three main subspecies, all occurring in both food and human sources. One cluster included all the dietary supplement strains, whereas genomes coming from food were predominantly spread across the other two groups.
Despite the high number of collected genomes (N = 369), Lb. plantarum was scarcely prevalent (1.8%) and abundant (av. 1.2%) in the gut (Fig. 1), which was reflected by only 11 MAGs being reconstructed from human microbiomes (Supplementary Fig. 7). All of these belonged to the main cluster (96% of the total genomes) associated with subsp. plantarum. A separate cluster was identified as subsp. argentoratensis, which was found in both food and human isolates but never reconstructed from metagenomes. The occurrence of multiple subspecies within the same SGB was also observed for eight additional LAB, i.e., Lb. brevis, Lb. fermentum, Lactobacillus johnsonii, Lactobacillus reuteri, Lb. sakei, L. lactis, L. mesenteroides, and W. cibaria, (Supplementary Fig. 7). On the other hand, Lc. garvieae was spread into two different SGBs, with one comprising human MAGs from both westernized and non-westernized populations and the other only from non-westernized cohorts (Supplementary Fig. 7). No genomes from food samples were collected at all for Lactobacillus crispatus, Lb. gasseri, Lactobacillus jensenii, Lb. ruminis, and Lb. salivarius (excluding a single isolate from ground beef). The non-food species Lb. ruminis and Lb. salivarius were quite prevalent in the gut with 145 and 42 MAGs reconstructed from human metagenomes, respectively (Supplementary Fig. 7). For both species, isolate and MAGs extracted from the gut were distinct from genomes isolated from other animal microbiomes, which suggested long-term adaptation of these species to the human gut. We also identified a specific Lb. salivarius cluster associated with dietary supplement strains, which was found in a couple of saliva samples but never in the human gut.
LAB occurrence in non-human primates is affected by captivity
We finally considered the set of 203 publicly available gut metagenomes from non-human primates (NHPs) that was recently retrieved, curated, and processed with the same pipeline employed in this study28. It comprised 22 host species from 14 different countries in five continents. Among the 2985 reconstructed MAGs, we found that only 46 of them (1.6%) were assigned to the Lactobacillales order (Supplementary Data 8), which suggested an overall low prevalence and abundance of LAB in the NHP gut microbiome. We found strong differences between MAGs retrieved from wild NHPs and those extracted from NHPs living in captivity. Wild NHPs generated 29 MAGs of LAB, with 66% of them associated with new species not available in public repositories and never found in human metagenomes, therefore likely representing bacteria peculiar to the NHP gut microbiomes. Ten MAGs were instead associated with kSGBs, with only five of them belonging to LAB species found also in human gut metagenomes such as Lc. garvieae (N = 3), Lc. lactis, and W. cibaria. Comparative genomics analysis highlighted that the strains harboured in NHPs were quite different from those reconstructed from human microbiomes (Supplementary Fig. 8). Interestingly, the three MAGs of Lc. garvieae resembled more the strains found in non-westernized human populations in terms of nucleotide identity. No MAGs from lactobacilli were extracted at all from wild NHPs. A very different situation was observed in captive NHPs (Supplementary Fig. 8), in which the 17 MAGs were exclusively reconstructed from kSGBs associated with multiple Lactobacillus species, i.e., Lb. acidophilus, Lactobacillus animalis (N = 2), Lb. johnsonii (N = 4), Lb. mucosae (N = 2), Lb. reuteri (N = 5), and Lb. salivarius (N = 3). Strains of Lb. reuteri and Lb. salivarius found in NHPs were distinct from those extracted from human and food sources, which suggested possible host adaptation mechanisms. A stronger overlap among NHPs, human, and food MAGs was instead observed for the other species and likely linked to the sharing of strains due to the exposition of NHPs living in captivity to human-like environments and diets40.
Discussion
In this study, we showed that food is likely the major source of LAB in the human gut microbiome. This was accomplished by conducting a large-scale meta-analysis that integrated taxonomic profiling and comparative genomics from almost ten thousand metagenomes from human and food sources in addition to reference genomes from public repositories. We focused the analysis on the thirty LAB that exhibited a prevalence >0.1% in the human gut, which resulted mainly in species of potential food origin, including LAB occurring in probiotic supplements, in addition to non-food origin species such as Lb. mucosae, Lb. ruminis, and Lb. salivarius. The comparative genomics suggested that closely related strains are present in both food and gut microbiome. Although such evidence does not exclude the possibility of other potential sources of LAB strains in the gut, we believe that being fermented foods the principal ecological niche for LAB in nature, our results support the hypothesis that food is the major source of LAB for the gut microbiome. While we considered the currently available taxonomic nomenclature, a substantial reclassification of the genus Lactobacillus into 25 novel genera enclosing the current Lactobacillus species was recently proposed41. The new Lactobacillus genus incorporates only the species included in the Lb. delbrueckii group.
We found an overall limited amount of LAB in the gut in terms of prevalence and relative abundance; however, several species exhibited non-negligible contributions that deserve attention for potential probiotic potentials. There was no evident correlation between prevalence and relative abundance of the different LAB species in the human samples. The most prevalent LAB species was S. thermophilus. Its role as a gut microbiome member is questioned. However, the mechanisms and metabolic features that lead to it being regarded as a candidate probiotic species have been studied and debated, especially in terms of resistance to gastrointestinal barriers and potential positive health effects42. Beyond being one of the two LAB widely employed for yoghurt making, S. thermophilus is also employed as starter cultures for many cheeses characterized by a thermophilic fermentation. Continuous exposure to S. thermophilus through cheese and yoghurt consumption can be a likely explanation of its prevalence in human gut samples as resulted in this study.
We detected a remarkable prevalence in the gut also for Lc. lactis, which is widespread in cheeses produced by mesophilic fermentation. Albeit recognized as a transient member of the gut community, higher levels of this species were found in buttermilk consumers43. In addition, strains of L. lactis have been shown to survive the gastrointestinal stress and this species can be considered to potentially convey health benefits by antimicrobial activity through bacteriocin production against clostridia, to boost the immune system, and to be potentially used as a vehicle of interesting beneficial properties such as antimicrobial activity44,45.
The prevalence of LAB in the human gut was strongly affected by lifestyle46, intended here as possible consumption of fermented foods that are characteristics of specific geographical regions. Unfortunately, direct associations of genomic data with dietary patterns could not be achieved as dietary records documenting systematic food consumption in the human public cohorts considered were not available. Minor associations between gut microbiota and consumption of plant fermented foods were very recently found within the American gut cohort. A few LAB species were linked to fermented plant food consumers and included Lb. acidophilus, Lb. brevis, Lactobacillus kefiranofaciens, Lactobacillus parabuchneri, Lb. helveticus, and Lb. sakei. Interestingly, the authors highlighted that the stool detection of LAB may be a useful tool to verify the reliability of self-reported dietary information on fermented foods consumption47.
In our study, LAB species widely occurring in dairy products and yoghurt, such as S. thermophilus and lactobacilli, were more prevalent in westernized populations, whereas the heterofermentative Leuconostoc and Weissella, likely carried as part of the epiphytic microbiota of raw vegetables23, fermented vegetables48, and cereal-based fermented foods49 were more common in the non-westernized cohorts. We could speculate that this pattern was linked to the habitual consumption of foods and diets that were characteristics of the specific geographical areas. For example, non-westernized populations that have a higher consumption of raw plants and plant based fermented foods were enriched in heterofermentative cocci LAB, whereas the very low prevalence of Lc. lactis and S. thermophilus in multiple Chinese cohorts reflects the low consumption of dairy products by the Chinese population50.
We conducted an extensive comparative genomic analysis by integrating reference genomes and MAGs from human, food, and environmental sources. This opportunity was previously prevented even for prevalent species such as S. thermophilus and Lc. lactis due to the lack of reference genomes acquired from human sources in public repositories. We identified a general overlap among genomes from food and gut sources, which suggested again food as the main source of LAB in the human gut. To this end, we conducted a preliminary analysis devoted to evaluate potential differences in functions of strains between food and gut sources, that we limited to Lc. lactis and S. thermophilus due to their large number of MAGs reconstructed in this study (see “Methods”). We found 266 (247 in food) and 323 (275 in food) differently prevalent genes (p < 0.05) for Lc. lactis and S. thermophilus, respectively, after removing genes encoding for unidentified functions or occurring redundantly in both food and gut groups (differently prevalent sugar metabolism genes are listed in Supplementary Data 9). However, such differences did not suggest remarkable potential functional differences between food and gut genomes, which was consistent with the comparative genomics and phylogenetic results shown in Fig. 3. At the same time, we identified an increase of unannotated genes in the gut genomes for both species, which agreed with the scarcity of reference genomes from human sources in public repositories. This may reflect further differences of strains found in the human gut that are currently unexplored due to the incompleteness of available functional databases51. Functional differences may suggest a possible adaptation of the food LAB to the gut environment. However, such mechanisms of adaptations cannot occur in strains that are part of a transient microbiome and would only take place for those LAB that more stably colonize the gut environment. This opens the need to conduct new analyses focused on the isolation of these microorganisms from the gut and their more in-depth functional characterization, also based on phenotypic traits. Different patterns were observed for typical non-food origin species such as Lb. ruminis and Lb. salivarius. By comparing human genomes with those found in other environments including animal microbiomes, we identified a strong adaptation of these species to the human gut, which suggested that these species are more specific and persistent for the human host (Supplementary Fig. 7).
Some of the analysed LAB exhibited distinct groups with human and food genomes clustering together, which indicated the presence in the gut of different strains potentially coming from different food sources. For example, the genomes of Lb. delbrueckii reconstructed from the gut appeared to cluster in two main groups associated with subsp. bulgaricus and subsp. lactis, which were representative of LAB in yoghurt and cheese, respectively. Multiple subclusters were identified also in Lb. rhamnosus, with only 17% of the reconstructed human MAGs corresponding to the strain GG largely used in probiotic supplements. These species, along with others such as Lb. casei, Lb. plantarum, and Lb. reuteri have been largely explored due to their probiotic potential. However, their general low prevalence and abundance in the human gut suggested that they are unlikely to be long-term residents of the gut microbiota. However, we used only fecal samples as representative of the gut microbiome, while such species maybe more tightly adhered to the gut epithelium and therefore less detectable in stool specimen52.
Finally, we highlight the importance of considering computational approaches such as those exploited in this paper. Strain-level genome comparison is fundamental to track the resilience and persistence of probiotic LAB in the human gut and can be a useful approach to be adopted in clinical trials aimed at evaluating the efficacy of microbial strains for gut health. In addition, the same methodologies can be considered to evaluate the prevalence and resilience of non-food microorganisms that are currently studied as candidate for next generation probiotics. Such knowledge and approaches can be useful for an informed design of functional foods, conveying health benefits upon daily consumption beyond their nutritional value. Several functional foods are enriched with probiotic microbial strains and their fate in pre-clinical and clinical trials can be efficiently and reliably monitored by culture-independent genome reconstruction and comparison to help assessing both their efficacy as probiotics and the quality of the functional food.
The interest in LAB will keep the scientific community active in studies of their genomics and evolution. Some of the LAB species occurring in the gut can surely arise from the consumption of fermented foods or probiotic preparations. However, efforts in research and isolation of LAB from human specimen would be desirable in the future in order to have further evidence on their specific genomic features that may better reflect adaptation to the complex gut ecosystem.
Methods
Publicly available and newly acquired food metagenomes
We considered and curated public datasets from fermented food metagenomes in addition to food metagenomes newly sequenced in this study. In total we put together 303 samples spanning 11 datasets and coming from different types of cheese (N = 191), fermented foods (N = 58), nunu (N = 20), milk kefir (N = 18), and yoghurt and dietary supplements (N = 16)8,9,10,11,12,13,14. More information is detailed in Table 1. Additional information on the collected food metagenomes is available in Supplementary Data 1.
Publicly available human metagenomes
In addition, we considered publicly available metagenomic datasets corresponding to the human microbiome. More specifically, we included 47 human microbiome datasets totalling 9,445 metagenomes and 4.2e11 Illumina reads as done in ref. 16 (17 metagenomes that were left out due to technical issues in ref. 16 were included here by marginally expanding the original set of 9428 metagenomes). Overall, the samples were acquired from six major body sites: the gut by stool sampling (N = 7907), oral cavity (N = 785), skin (N = 508, including from the anterior nares), airways (N = 151), vagina (N = 86), and breast milk (N = 8, data not included in figures). These samples covered 31 countries that were grouped by continent as follows: Africa (MDG: Madagascar, TZA: Tanzania), Asia (BGD: Bangladesh, BRN: Brunei, IDN: Indonesia, ISR: Israel, KAZ: Kazakhstan, MNG: Mongolia, MYS: Malaysia, SGP: Singapore), China (CHN, which we kept separated from the other Asian countries due to its large sample size), Europe (AUT: Austria, DEU: Germany, DNK: Denmark, ESP: Spain, EST: Estonia, FIN: Finland, FRA: France, GBR: Great Britain, HUN: Hungary, ISL: Iceland, ITA: Italy, NLD: The Netherlands, NOR: Norway, RUS: Russia, SVK: Slovakia, SWE: Sweden), North America (CAN: Canada, USA: United States), Oceania (FJI: Fiji), and South America (PER: Peru). The samples were also categorized as corresponding to westernized (N = 8850) and non-westernized (N = 595) lifestyles16. More specifically, westernization is a complex process that occurred during the last few centuries and that involved lifestyle changes compared with populations prior to the modern era. Such changes include increased hygiene and sanitized environments, introduction of antibiotics and other drugs, increased high-calorie high-fat dietary regimes, enhanced exposure to pollutants, and reduced contact with wildlife and domesticated animals. We adopt westernized and non-westernized as umbrella terms to depict populations that differ by the majority of the aforementioned factors even though this definition comprises heterogeneous populations. Finally, these metagenomes spanned multiple age categories: newborns (N = 711, <1 year of age), children (N = 802, age ≥ 1 and <12 years), school age individuals (N = 215, age ≥ 12 and <19 years), and adults (N = 7669, age ≥ 19 years). Despite curation efforts, age category metadata corresponding to 48 samples could not be sourced. These manually curated metadata are available in the Supplementary Data 2 and in the curatedMetagenomicData package15.
Taxonomic profiling of food and human metagenomes
Quantitative taxonomic profiling was applied on the 9,445 human metagenomes and the 303 food metagenomes by applying MetaPhlAn217 with default parameters. MetaPhlAn2 estimates relative abundances of microbial species using the pre-generated ~1 M unique clade-specific marker genes identified from ~17,000 reference genomes (~13,500 bacterial and archaeal, ~3500 viral, and ~110 eukaryotic). Taxonomic profiles along with associated metadata information are available in Supplementary Data 2. We detected 152 species belonging to the Lactobacillales order occurring in at least one of the metagenomes with a relative abundance >0.01%. Among them, we identified 70 species belonging to the LAB group (i.e., species belonging to Lactobacillus, Lactococcus, Leuconostoc, and Weissella genera in addition to S. thermophilus), and restricted the rest of the analysis to the 30 of them having a prevalence >0.1% in the human gut. Taxonomic profiles of these 30 species are reported in Fig. 1 and Supplementary Figs. 1–4. Prevalence was computed by thresholding relative abundance at 0.01%. Average relative abundance was computed on positive samples only.
Metagenome-assembled genomes reconstruction
Taxonomic profiling was coupled with the reconstruction of microbial genomes directly from metagenomes. The approach that we validated in16 was applied here to reconstruct MAGs MAGs from food metagenomes. More specifically, single-sample metagenomics assemblies were generated with metaSPAdes53 (version 3.10.1; default parameters) or IDBA-UD54 (version 1.1.3; default parameters). Contigs longer than 1,000 nt were binned with MetaBAT255 (version 2.12.1; option “-m 1500”). Quality control with CheckM (v. 1.0.7)56 yielded 666 medium-quality food MAGs (completeness > 50% and contamination <5%). These newly reconstructed MAGs were then considered within the human MAG catalogue totalling 154,723 MAGs reconstructed from the 47 human datasets considered in this study16.
Clustering of genomes into species-level genome bins
The 155,389 MAGs described in the previous section were integrated with the set of 193,078 reference genomes available in GenBank as of March 2019. This resulted in a total of 348,467 genomes that were clustered into SGBs following the procedure proposed in16. Genomes were clustered with average linkage at 5% genetic distance based on whole-genome nucleotide similarity estimation using Mash (v. 2.0; option “-s 10000” for sketching)57. The 666 food MAGs were grouped by this procedure into 171 SGBs: 108 SGBs (comprising 574 MAGs) contained at least one reference genome or human MAG (kSGBs), while a further 63 SGBs (comprising 92 MAGs) consisted only of genomes reconstructed in this study from food metagenomes (fSGBs). Summaries of the newly generated MAGs and SGBs are available in Fig. 2a, b and Supplementary Data 5 and 6.
Metadata curation for selected LAB species
We considered the 30 selected LAB species shown in Fig. 1 for comparative genomics purposes. Among the 348,467 genomes described in the previous section, 2859 genomes (comprising 1042 MAGs) were included in SGBs containing at least one reference genome assigned to these 30 species and were kept for further analyses. We retrieved and manually curated the source type in all cases. For reference genomes, the source of isolation was extracted from the NCBI portal or from related publications. Genomes were grouped in three categories based on the source type: “human,” “food,” and “other.” Genomes for which this information was missing were labelled as “NA” (N = 226, 7.9% of the cases). More information relating to these 2859 genomes is available in Supplementary Data 7.
Reconstruction of phylogenetic structure
Phylogenies were built using the newly developed PhyloPhlAn 3.0 package that extends the original PhyloPhlAn2 version58. Each SGB-specific phylogeny (Fig. 3) was based on the set of species-specific marker genes that can be retrieved in PhyloPhlan 3.0 with the command phylophlan2_setup_database.py. The number of marker genes for each SGB is summarized in Supplementary Data 10. This departs from the default option in using the 400 universal markers available in PhyloPhlAn 3.0 and guarantees a higher resolution of the built phylogenies. The parameters were set as follows “--diversity low --fast --min_num_marker 50”, which indicated that genomes mapping less than 50 markers were discarded from the phylogeny. External tools embedded in PhyloPhlan 3.0 were run with their specific options as follows:
-
blastn (version 2.6.0 + ;59) with parameters “-outfmt 6 -max_target_seqs 1000000”
-
mafft (version 7.310;60) using the “L-INS-i” algorithm and with parameters “--anysymbol --auto”
-
trimal (version 1.2rev59;61) with parameter “-gappyout”
-
FastTree (version 2.1.9;62) with parameters “-mlacc 2 -slownni -spr 4 -fastest -mlnni 4 -no2nd -gtr -nt”
-
RAxML (version 8.1.15;63) with parameters “-p 1989 -m GTRCAT -t <phylogenetic tree computed by FastTree > ”
Phylogenetic trees (Figs. 3 and 4) were visualized with GraPhlAn64. In addition, multidimensional scaling plots (Figs. 3 and 4, and Supplementary Figs. 4 and 5) were built on the whole-genome Average Nucleotide Identity distances computed with FastANI65.
Functional analysis and statistical significance
The set of genomes (MAGs and reference genomes) considered in this study was annotated with Prokka (v. 1.12;66) using default parameters. Proteins inferred by Prokka were then processed with Roary67 (v. 3.11; option ‘-i 90’) to generate the presence–absence binary matrix on the core and accessory genes. Gene enrichment within human and food genomes was determined by considering only MAGs and reference genomes having completeness >80% in order to avoid possible biases coming from highly incomplete genomes and by taking into account genes present in at least 5% and <95% of the genomes. Statistical significance was tested through Fisher’s test with false discovery rate correction for multiple hypothesis testing.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The raw data for the food metagenomes are available in NCBI-SRA under the BioProjects PRJEB6952 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB6952], PRJEB15423 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB15423], PRJEB15432 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB15432], PRJEB20873 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB20873], PRJEB32768 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB32768], PRJEB35321 [https://www.ncbi.nlm.nih.gov/bioproject/PRJEB35321], PRJNA286900 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA286900], PRJNA430402 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA430402], PRJNA482503 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA482503], PRJNA603575 [https://www.ncbi.nlm.nih.gov/bioproject/PRJNA603575], and in MG-RAST under the Project mgp3362 [https://www.mg-rast.org/linkin.cgi?project=mgp3362].
The taxonomic profiles with associated metadata from the human metagenomes are available in the curatedMetagenomicData package15. The MAGs from human metagenomes are available at http://segatalab.cibio.unitn.it/data/Pasolli_et_al.html. The newly reconstructed MAGs from food metagenomes are available at http://www.tfm.unina.it/DATA001-2020-Pasolli.
References
Cordain, L. et al. Origins and evolution of the Western diet: health implications for the 21st century. Am. J. Clin. Nutr. 81, 341–354 (2005).
Marco, M. L. et al. Health benefits of fermented foods: microbiota and beyond. Curr. Opin. Biotechnol. 44, 94–102 (2017).
Stefanovic, E., Fitzgerald, G. & McAuliffe, O. Advances in the genomics and metabolomics of dairy lactobacilli: a review. Food Microbiol. 61, 33–49 (2017).
Wu, C., Huang, J. & Zhou, R. Genomics of lactic acid bacteria: Current status and potential applications. Crit. Rev. Microbiol. 43, 393–404 (2017).
Douillard, F. P. & de Vos, W. M. Biotechnology of health-promoting bacteria. Biotechnol. Adv. 37, 107369 (2019).
Hill, C. et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11, 506–514 (2014).
Derrien, M. & van Hylckama Vlieg, J. E. T. Fate, activity, and impact of ingested bacteria within the human gut microbiota. Trends Microbiol. 23, 354–366 (2015).
Bertuzzi, A. S. et al. Omics-based insights into flavor development and microbial succession within surface-ripened cheese. mSystems 3, e00211-17 (2018).
Escobar-Zepeda, A., Sanchez-Flores, A. & Quirasco Baruch, M. Metagenomic analysis of a Mexican ripened cheese reveals a unique complex microbiota. Food Microbiol. 57, 116–127 (2016).
Milani, C. et al. Colonization of the human gut by bovine bacteria present in Parmesan cheese. Nat. Commun. 10, 1286 (2019).
Quigley, L. et al. Thermus and the pink discoloration defect in cheese. mSystems 1, e00023-16 (2016).
Walsh, A. M. et al. Microbial succession and flavor production in the fermented dairy beverage kefir. mSystems 1, e00052-16 (2016).
Walsh, A. M. et al. Strain-level metagenomic analysis of the fermented dairy beverage nunu highlights potential food safety risks. Appl. Environ. Microbiol. 83, e01144-17 (2017).
Wolfe, B. E., Button, J. E., Santarelli, M. & Dutton, R. J. Cheese rind communities provide tractable systems for in situ and in vitro studies of microbial diversity. Cell 158, 422–433 (2014).
Pasolli, E. et al. Accessible, curated metagenomic data through ExperimentHub. Nat. Methods 14, 1023–1024 (2017).
Pasolli, E. et al. Extensive unexplored human microbiome diversity revealed by over 150,000 genomes from metagenomes spanning age, geography, and lifestyle. Cell 176, 649–662.e20 (2019).
Truong, D. T. et al. MetaPhlAn2 for enhanced metagenomic taxonomic profiling. Nat. Methods 12, 902–903 (2015).
Redondo-Useros, N. et al. Associations of probiotic fermented milk (PFM) and yogurt consumption with Bifidobacterium and Lactobacillus components of the gut microbiota in healthy adults. Nutrients 11, 651 (2019).
Vatanen, T. et al. Variation in microbiome LPS immunogenicity contributes to autoimmunity in humans. Cell 165, 1551 (2016).
Soto, A. et al. Lactobacilli and bifidobacteria in human breast milk: influence of antibiotherapy and other host and clinical factors. J. Pediatr. Gastroenterol. Nutr. 59, 78–88 (2014).
Murphy, K. et al. The Composition of human milk and infant faecal microbiota over the first three months of life: a pilot study. Sci. Rep. 7, 40597 (2017).
Ferretti, P. et al. Mother-to-infant microbial transmission from different body sites shapes the developing infant gut microbiome. Cell Host Microbe 24, 133–145.e5 (2018).
Di Cagno, R., Coda, R., De Angelis, M. & Gobbetti, M. Exploitation of vegetables and fruits through lactic acid fermentation. Food Microbiol. 33, 1–10 (2013).
Broussard, J. L. & Devkota, S. The changing microbial landscape of Western society: Diet, dwellings and discordance. Mol. Metab. 5, 737–742 (2016).
Schnorr, S. L. et al. Gut microbiome of the Hadza hunter-gatherers. Nat. Commun. 5, 3654 (2014).
Crittenden, A. N. & Schnorr, S. L. Current views on hunter-gatherer nutrition and the evolution of the human diet. Am. J. Phys. Anthropol. 162, 84–109 (2017).
Zhu, K. et al. Anti-obesity effects of Lactobacillus fermentum CQPC05 isolated from Sichuan pickle in high-fat diet-induced obese mice through PPAR-α signaling pathway. Microorganisms 7, 194 (2019).
Manara, S. et al. Microbial genomes from non-human primate gut metagenomes expand the primate-associated bacterial tree of life with over 1000 novel species. Genome Biology 20, 299 (2019).
Bowers, R. M. et al. Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea. Nat. Biotechnol. 35, 725 (2017).
Roos, S., Karner, F., Axelsson, L. & Jonsson, H. Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. Int. J. Syst. Evol. Microbiol. 50(Pt 1), 251–258 (2000).
Heredia, N. & García, S. Animals as sources of food-borne pathogens: a review. Anim. Nutr. 4, 250–255 (2018).
Martin, N. H., Trmčić, A., Hsieh, T.-H., Boor, K. J. & Wiedmann, M. The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Front. Microbiol. 7, 1549 (2016).
Zanine, A. M. et al. Effects of Streptococcus bovis Isolated from bovine rumen on the fermentation characteristics and nutritive value of Tanzania grass silage. Sci. World J. 2016, 8517698 (2016).
Beck, M., Frodl, R. & Funke, G. Comprehensive study of strains previously designated Streptococcus bovis consecutively isolated from human blood cultures and emended description of Streptococcus gallolyticus and Streptococcus infantarius subsp. coli. J. Clin. Microbiol. 46, 2966–2972 (2008).
Godon, J. J., Delorme, C., Ehrlich, S. D. & Renault, P. Divergence of genomic sequences between Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris. Appl. Environ. Microbiol. 58, 4045–4047 (1992).
Almeida, A. et al. A new genomic blueprint of the human gut microbiota. Nature 568, 499–504 (2019).
Nayfach, S., Shi, Z. J., Seshadri, R., Pollard, K. S. & Kyrpides, N. C. New insights from uncultivated genomes of the global human gut microbiome. Nature 568, 505–510 (2019).
Segers, M. E. & Lebeer, S. Towards a better understanding of Lactobacillus rhamnosus GG–host interactions. Microb. Cell Fact. 13(Suppl 1), S7 (2014).
Wuyts, S. et al. Large-scale phylogenomics of the Lactobacillus casei group highlights taxonomic inconsistencies and reveals novel clade-associated features. mSystems 2, e00061–17 (2017).
Li, X. et al. Establishment of a Macaca fascicularis gut microbiome gene catalog and comparison with the human, pig, and mouse gut microbiomes. Gigascience 7, giy100 (2018).
Zheng, J. et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 10.1099/ijsem.0.004107 (2020).
Uriot, O. et al. Streptococcus thermophilus: from yogurt starter to a new promising probiotic candidate? J. Funct. Foods 37, 74–89 (2017).
Zhernakova, A. et al. Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science 352, 565–569 (2016).
Song, A. A.-L., In, L. L. A., Lim, S. H. E. & Rahim, R. A. A review on Lactococcus lactis: from food to factory. Microb. Cell Fact. 16, 55 (2017).
Nakamura, S., Morimoto, Y. V. & Kudo, S. A lactose fermentation product produced by Lactococcus lactis subsp. lactis, acetate, inhibits the motility of flagellated pathogenic bacteria. Microbiology 161, 701–707 (2015).
Food and Agriculture Organization of the United Nations. New Food Balances. http://www.fao.org/faostat/en/#data/FBS. Accessed: 1st February 2020.
Taylor, B. C. et al. Consumption of fermented foods is associated with systematic differences in the gut microbiome and metabolome. mSystems 5, e00901–19 (2020).
Jung, J. Y. et al. Metagenomic analysis of kimchi, a traditional Korean fermented food. Appl. Environ. Microbiol. 77, 2264–2274 (2011).
Tamang, J. P., Watanabe, K. & Holzapfel, W. H. Review: Diversity of microorganisms in global fermented foods and beverages. Front. Microbiol. 7, 377 (2016).
Prentice, A. M. Dairy products in global public health. Am. J. Clin. Nutr. 99, 1212S–1216SS (2014).
Thomas, A. M. & Segata, N. Multiple levels of the unknown in microbiome research. BMC Biol. 17, 48 (2019).
Hemarajata, P. & Versalovic, J. Effects of probiotics on gut microbiota: mechanisms of intestinal immunomodulation and neuromodulation. Ther. Adv. Gastroenterol. 6, 39–51 (2013).
Nurk, S., Meleshko, D., Korobeynikov, A. & Pevzner, P. A. metaSPAdes: a new versatile metagenomic assembler. Genome Res. 27, 824–834 (2017).
Peng, Y., Leung, H. C. M., Yiu, S. M. & Chin, F. Y. L. IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth. Bioinformatics 28, 1420–1428 (2012).
Kang, D. D. et al. MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies. PeerJ 7, e7359 (2019).
Parks, D. H., Imelfort, M., Skennerton, C. T., Hugenholtz, P. & Tyson, G. W. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 25, 1043–1055 (2015).
Ondov, B. D. et al. Mash: fast genome and metagenome distance estimation using MinHash. Genome Biol. 17, 132 (2016).
Asnicar, F. et al. Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0. Nat. Commun. (in the press).
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403–410 (1990).
Katoh, K. & Standley, D. M. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30, 772–780 (2013).
Capella-Gutiérrez, S., Silla-Martínez, J. M. & Gabaldón, T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25, 1972–1973 (2009).
Price, M. N., Dehal, P. S. & Arkin, A. P. FastTree 2–approximately maximum-likelihood trees for large alignments. PLoS ONE 5, e9490 (2010).
Stamatakis, A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313 (2014).
Asnicar, F., Weingart, G., Tickle, T. L., Huttenhower, C. & Segata, N. Compact graphical representation of phylogenetic data and metadata with GraPhlAn. PeerJ 3, e1029 (2015).
Jain, C., Rodriguez-R, L. M., Phillippy, A. M., Konstantinidis, K. T. & Aluru, S. High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat. Commun. 9, 5114 (2018).
Seemann, T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30, 2068–2069 (2014).
Page, A. J. et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 31, 3691–3693 (2015).
Acknowledgements
This study was developed within the project MASTER (Microbiome Applications for Sustainable food systems through Technologies and Enterprise). The project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 818368. This manuscript reflects only the authors’ views and the European Commission is not responsible for any use that may be made of the information it contains. The study has been also supported by the JPI HDHL-INTIMIC - Knowledge Platform of Food, Diet, Intestinal Microbiomics and Human Health, granted by the Italian Ministry of Agricultural, Food and Forestry and Tourism Policies with ID 790.
Author information
Authors and Affiliations
Contributions
Conception: D.E. and E.P. Formal analysis: E.P., F.D.F., I.E.M., A.W., J.L., and F.C. Resources and funding acquisition: D.E., N.S., and P.C. Writing–original draft, E.P. and D.E. Writing–review and editing, E.P., D.E., N.S., and P.C. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Peer review information Nature Communications thanks Dirk Haller, Anna Heintz-Buschart and the other, anonymous, reviewer for their contribution to the peer review of this work. Peer reviewer reports are available.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Pasolli, E., De Filippis, F., Mauriello, I.E. et al. Large-scale genome-wide analysis links lactic acid bacteria from food with the gut microbiome. Nat Commun 11, 2610 (2020). https://doi.org/10.1038/s41467-020-16438-8
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-020-16438-8
This article is cited by
-
Fermented foods and gastrointestinal health: underlying mechanisms
Nature Reviews Gastroenterology & Hepatology (2024)
-
Multifactorial inhibition of Candida albicans by combinations of lactobacilli and probiotic Saccharomyces cerevisiae CNCM I-3856
Scientific Reports (2024)
-
Next-generation probiotics: the upcoming biotherapeutics
Molecular Biology Reports (2024)
-
Assessment of the in-vitro probiotic efficacy and safety of Pediococcus pentosaceus L1 and Streptococcus thermophilus L3 isolated from Laban, a popular fermented milk product
Archives of Microbiology (2024)
-
Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient
Biotechnology and Bioprocess Engineering (2024)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.