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Hidden heterogeneity and circadian-controlled cell fate inferred from single cell lineages

Abstract

The origin of lineage correlations among single cells and the extent of heterogeneity in their intermitotic times (IMT) and apoptosis times (AT) remain incompletely understood. Here we developed single cell lineage-tracking experiments and computational algorithms to uncover correlations and heterogeneity in the IMT and AT of a colon cancer cell line before and during cisplatin treatment. These correlations could not be explained using simple protein production/degradation models. Sister cell fates were similar regardless of whether they divided before or after cisplatin administration and did not arise from proximity-related factors, suggesting fate determination early in a cell’s lifetime. Based on these findings, we developed a theoretical model explaining how the observed correlation structure can arise from oscillatory mechanisms underlying cell fate control. Our model recapitulated the data only with very specific oscillation periods that fit measured circadian rhythms, thereby suggesting an important role of the circadian clock in controlling cellular fates.

Introduction

Elucidating the mechanisms of cell cycle control has been one of the most important endeavors in cell biology over the last decades. Since the seminal discoveries of the cdc and wee genes in yeast and the introduction of the idea of cell cycle checkpoints1,2,3, much effort has been devoted to characterizing the genes and proteins that act in concert to regulate the cell cycle4. An important breakthrough in this regard has been the recognition that the circadian rhythm likely plays a crucial role in cell cycle control. While historically the cell cycle has been considered to be independent of the circadian clock, there is emerging evidence that these two processes may be intricately connected, with the circadian clock providing an extra layer of control on the cell cycle5,6,7. Not surprisingly, the coupling between the circadian clock, cell cycle and cell death pathways (or the lack thereof) has major implications for anti-cancer therapies8,9,10, and forms the basis of the emerging field of cancer chronotherapy11. Whether any coupling exists in different cancer types, the possible phenotypic outcomes of such a coupling, and how it can potentially drive heterogeneous cellular responses to cancer therapies remain fundamental questions to be addressed.

A recent study12 proposed that correlation structures in the inter-mitotic times (IMT) of cells, which have been observed in several experiments over the past decades12,13,14,15,16,17, could be generated as a result of circadian gating of the cell cycle. The origin of these intricate correlation structures among cellular lineages has been the subject of intense study, since they are expected to act as key probes into the underlying biochemical and physical processes governing cell cycle dynamics12,13,14,15,16,17,18. The recently proposed circadian model can in principle capture the observed correlations in IMT, including the widely varying mother–daughter relationships and the so called cousin–mother inequality12,19 (where the cousin correlation in IMT is greater than the mother–daughter correlation), but it does not account for the distinct shapes of IMT distributions that have consistently been observed in previous studies20,21. Inferring these distributions from single cell data is a challenging task in scenarios with multiple possible fates due to biases introduced in the observed data as a result of stochastic competition among cellular fates22. Current methods of inferring these distributions do not account for this competition effect20, and hence are applicable only in limited scenarios where a single fate dominates—for example when drug concentrations are very low or very high. In addition, there is evidence for the existence of strong correlations among times to death of sister and cousin cells22,23,24,25,26. However, all previous computational approaches describe mechanisms that specifically explore correlations in either IMT or apoptosis times (AT), and do not provide a unified approach to explain the experimental observations in a comprehensive manner. Existing models therefore cannot explain the entire set of observations obtained from single cell lineage tracking experiments.

Here we set out to design an integrative method to address these fundamental issues. We generated single cell lineage tracking data of human colorectal cancer cells (HCT116), both in the absence and presence of the chemotherapeutic agent cisplatin, to explore lineage correlation structures in IMT and AT of cells. We found complex correlation structures both in IMT and AT, which depend on the degree of relatedness of the cells. Interestingly, we also found that related cells display a large degree of similarity in p53 dynamics and cell fate after cisplatin treatment, providing strong evidence that cellular heterogeneity prior to drug treatment predisposes cells to specific fates. This result is reminiscent of previous work on TRAIL-induced apoptosis24 and proliferation-quiescence fate choices in cells27,28, and suggests that heterogeneous levels of proteins passed on from mother to daughter cells can to a large extent determine cell fates early in the daughter cell’s lifetime. Based on this result, we developed a theoretical model in which the phase of a cellular oscillator at the time when a mother cell divides controls eventual cell fate probabilities in the daughters. To investigate the ability of this theory to explain our experimental observations, we developed two computational algorithms: (1) a general statistical method to quantify the large extent of drug-induced hidden heterogeneities in IMT, which cannot be directly observed in the data due to stochastic competition between cell division and death events22, and (2) a computational algorithm to mimic single cell lineage tracking experiments allowing for oscillatory control of cell fates. We showed that this integrative method, using a minimal set of tunable parameters, can explain the entirety of the correlation structures in addition to accounting for hidden heterogeneities. Importantly, using the same theoretical formulation while switching to physically realistic but non-oscillatory models of cell fate control failed to recapitulate the cousin-mother inequality. In addition, our model was not able to reproduce the correlation structures for most values of the oscillation period, except for a period of 24 h and a few other multiples of 12 h such as 12 and 48 h. Our work therefore suggests an important role of the circadian clock in controlling times to cellular fates, both in the presence and absence of drugs, and provides a widely applicable method for correctly inferring heterogeneities in times to cell fate from single cell data.

Results

Correlation structures before and after cisplatin treatment

In order to obtain accurate single cell lineage data on cell fates and times to cell fates, we used HCT116 p53-VKI human colon cancer cells, a previously established clonal cell line in which one allele of the endogenous TP53 gene is tagged with the Venus fluorescent protein29. We imaged untreated, proliferating HCT116 p53-VKI cells for two days, followed by a switch to fresh media with 12.5 μM cisplatin. Time lapse microscopy and lineage tracking was then continued for another three days after cisplatin administration, and times at which cell divisions and death events took place were recorded throughout (Fig. 1a, Supplementary Figure 14 and 15, Supplementary Movie 1). Intermitotic and apoptosis times (IMT and AT, respectively) were defined from the time a cell was born to the time of mitosis or death (Fig. 1b). We classified these events into three categories—events that occur entirely before the time of cisplatin administration (Td), events that straddle Td, and those that occur after Td (Fig. 1b).

By computing correlation structures in times to division before cisplatin administration (Fig. 1c–e, Supplementary section 1), we found that the mother–daughter correlation in IMT is insignificantly different from 0 (Pearson correlation, $$\rho \sim - 0.03$$ for 71 pairs, P-val [t-test] = 0.7, 95% CI [−0.26, 0.16]), sister correlations are large ($$\rho \sim 0.73$$ for 80 sister pairs, P-val [t-test] = 2.9 × 10−14, 95% CI [0.6, 0.8]), and the cousin–mother inequality12,30 (where the cousin correlations are larger than the mother–daughter correlations) is satisfied (ρ ~ 0.34 for 46 cousin pairs, P-val [t-test] = 0.02, 95% CI [0.1, 0.57]). Here sister cells are defined as cells with the same mother while cousins are cells whose mothers were sisters. For division events straddling Td (red cells in Fig. 1b), we observed similar correlations among sisters and cousins, though smaller in magnitude (Supplementary section 1, Supplementary Figure 1). Note that for these events mother–daughter relationships are not defined, since the mothers are not part of this category. The apoptosis times (AT) of sister and cousin pairs of cells treated with cisplatin (red and green cells that die in Fig. 1b) also show significantly positive correlations (Fig. 1f, g; $$\rho \sim 0.64$$ for 93 sister pairs, P-val [t-test] = $$3.09 \times 10^{ - 12}$$, 95% CI [0.48, 0.78]; $$\rho \sim 0.38$$ for 60 cousin pairs, P-val [t-test] = 0.001, 95% CI [0.15, 0.54]).

We then explored correlations in cell fates after cisplatin administration (Fig. 2a–b). We found that sister cells shared the same fate (death or survival) about 80% of the time, regardless of whether sisters divided before or after cisplatin treatment (Fig. 2a, b). If cell fates were independent, sisters would be expected to share the same fate ~53% of the time (see Supplementary section 2 for calculations). The similarity in fates of related cells diminished with increasing numbers of divisions separating the cells (Supplementary Figure 2a). Cells separated by four divisions (3rd cousins) shared the same fate in similar proportions to unrelated cells. We observed similar trends when cell division following cisplatin treatment was also incorporated into cell fate considerations (Supplementary Figure 2b, c). To rule out possible spatial effects, such as similar cisplatin exposure levels of physically proximal cells driving the similarity in sister fates, we measured distances between related cells. Though sister cells tend to be close together in space (Fig. 2c), unrelated cells separated by similar distances do not exhibit the same degree of similarity in fates (Fig. 2d). This observation suggests that shared fate is not the result of proximity-related factors but rather cell-intrinsic factors that predispose cells to a particular fate. Using a geminin reporter, we ruled out potential connections between cell cycle stage at the time of cisplatin treatment and cell death (Supplementary section 3, Supplementary Figure 3). However, cells in G1 during cisplatin treatment were more likely to remain arrested following treatment than cells in G2/M (Supplementary Figure 3i). Finally, we found that p53 dynamics was correlated between related cells (Fig. 2e, f) and was also correlated with the time to death (Supplementary Figure 3h), consistent with our previous work on cisplatin-induced cell fates being associated with p53 dynamics29. Taken together, our results suggest that the state of a cell prior to cisplatin exposure, likely inherited from its mother during mitosis, affects the rate of p53 accumulation and predisposes it to a specific cell fate. This finding motivated the development of our birth-death process models and lineage simulations, as discussed below.

A statistical algorithm to quantify hidden heterogeneity

To develop a mechanistic understanding of these lineage correlation structures in HCT116 cells, it is crucial to correctly quantify and account for the large heterogeneities in IMT and AT. However, this is a challenging task in the presence of multiple competing cellular fates22. The true underlying distributions governing cell division and death processes are masked due to stochastic competition between the fates, and the observed experimental data (Fig. 3a–c) may therefore be very different compared to the true underlying distributions22. The cause of this bias is the mutual exclusivity of cellular fates—the only fate that is observed is the one that happens to occur earlier. Hence values chosen from the right tails of the true IMT and AT distributions are unlikely to be observed due to the earlier occurrence of the competing fate. As a result, the observed times to both division and death are skewed towards shorter times; the extent of this bias depends on how much the underlying IMT and AT distributions overlap.

In order to infer the correct underlying distributions of IMT and AT, we developed a computational framework to model the times to cell fates in the single cell lineage data, accounting for the large sister correlations. In brief, we described the entire dataset (the single cell data is provided as Supplementary Data 1 with a detailed explanation of the data structure in Supplementary Data 2) as a collection of sister pairs with concordant or discordant fates, and designed a likelihood function to compute the probability of observing the data. The basic form of the likelihood function for one sister pair is given by:

$$f_i\left( {t_1^i,t_2^i;{\mathbf{\theta }}} \right) = c_z\left( {1 - S^i\left( {t_1^i} \right),1 - S^i\left( {t_2^i} \right)} \right)S^i\left( {t_1^i} \right)h\left( {t_1^i;{\mathbf{\theta }}} \right)S^i\left( {t_2^i} \right)h\left( {t_2^i;{\mathbf{\theta }}} \right).$$
(1)

Here $$f_i\left( {t_1^i,t_2^i} \right)$$ is the bivariate joint probability density of observing the first sister cell in the ith pair to divide or die at time $$t_1^i$$ after its birth, and the second cell of that sister pair to divide or die at time $$t_2^i$$ since birth; $$S^i\left( {t_1^i} \right)$$ and $$S^i\left( {t_2^i} \right)$$are the univariate survival functions of the sisters, denoting their probabilities to survive until times $$t_1^i$$ and $$t_2^i$$, respectively; $$h\left( {t_1^i;{\mathbf{\theta }}} \right)$$ and $$h\left( {t_2^i;{\mathbf{\theta }}} \right)$$ are the univariate hazard functions of the sisters, representing their risks of dividing (or dying) at times $$t_1^i$$ and $$t_2^i$$, respectively (see Supplementary section 5 for details); and θ is the vector of parameters to be inferred from the data; it depends on the functional form chosen to represent the variability in IMT and AT. We used the Exponentially Modified Gaussian (EMG) function for this purpose, since this function was found to best describe our observed data (Supplementary section 4, Supplementary Tables 1-3). The EMG has also previously been shown to better explain cell division time variability than other commonly used functions20,21. The EMG is a convolution of a Gaussian with parameters μ,σ and an exponential with parameter λ. Finally, we accounted for the large sister correlations by using a copula cz, which is a function that joins together one-dimensional density functions to form a multivariate density function31 (Supplementary section 5). We used a Gaussian copula throughout this work, parameterized by the single parameter z, which represents the Pearson correlation between sister cells. We modeled stochastic competition among cellular fates using a competing risks framework. The full likelihood function is a product of Supplementary Equation (1) over all sister pairs in the dataset. Further details of the model are provided in Supplementary section 5. We observed that accounting for correlations among sisters led to significant improvements in the estimation of the distribution parameters using a simulation approach as well as direct observation of the pre-cisplatin IMT data (Supplementary section 5, Supplementary Figure 4). We also accounted for the cells that survive until the end of the experiment or 72 h after cisplatin treatment, and allowed for the possibility of a delay between the time of drug administration and the realization of its effect on cell fates30 (Supplementary section 5). Copulas, while commonly used in finance32, have rarely been used in biology. Our results highlight the usefulness of this method for modeling correlated data in this and potentially other biological contexts.

Our computational framework was first used to identify the underlying IMT distribution of HCT116 cells in the absence of cisplatin. Since there was very little cell death in this scenario, the inferred IMT distribution should be almost identical to a histogram of the IMT data, which is indeed what we found (parameters of the EMG function in Supplementary section 4 and Supplementary Table 4). In addition, since there are a relatively large number of IMT pairs available in the data (80 pairs), a direct calculation of the Pearson correlation of sisters from the data should also be close to the inferred value. As expected, the inferred sister–sister correlation of 0.71 (Supplementary Table 4; standard error calculated as the square root of the parameter variance, computed from the Hessian matrix) was within error identical to the directly calculated value of 0.73 (Fig. 1d). These results provide a direct validation of our inference procedure. The bivariate density of the sisters is also captured well by the copula framework with inferred univariate EMG margins and the inferred Pearson correlation, as demonstrated in Supplementary Figure 5.

We then inferred the drug-induced distributions of IMT and AT, accounting for the measured sister correlations in IMT and AT using the copula framework (Fig. 3d, e; inferred parameters given in Supplementary Table 5 and Supplementary Table 6). Remarkably, we found that the underlying IMT distribution is very different from the distribution obtained directly by binning the data (Fig. 3d): the directly computed mean of the division times of cells that straddle the dosing event is 20 h as opposed to the inferred mean of 47.22 h (Fig. 3d). Similarly, the standard deviation of the observed histogram is 5.65 h, underestimating the inferred but “hidden” heterogeneity with a standard deviation of 33.05 h (Fig. 3d). Current methods for analyzing this kind of single cell data that treat cell division and death independently20 would therefore severely underestimate the effects of the drug. The inferred distribution of AT (Fig. 3e) is also shifted, though not as much as the IMT distribution, as expected (see Supplementary Section 5 for a detailed discussion).

To independently confirm these results, we used the inferred IMT and AT distributions from Fig. 3d, e as inputs to a stochastic, age-dependent birth-death process simulation of cellular proliferation33. Following single cells over time, we generated stochastic waiting times to division or death of each cell based on the hazard functions corresponding to the input IMT and AT distributions (Supplementary section 6). A hazard function, as outlined in the context of Supplementary Equation (1), represents the risk of a cell dividing or dying at any point in time, given that it has survived until that time. The results of these simulations provide the post-competition IMT and AT histograms (Fig. 3f, g). We observed a close match between the predicted IMT distribution and experimental results (Fig. 3f), providing a confirmation of our inferences. A similarly close match was obtained for the AT distribution (Fig. 3g). Conversely, if the measured IMT and AT distributions (Fig. 3b, c, respectively) were instead used as inputs to the simulation, the results were not found to match the experimental data (Supplementary Figure 6). This observation arises because the observed data only exhibit the post-competition IMT and AT distributions and do not represent the true underlying distributions that generate the observed data. This finding highlights the importance of using an integrative analysis approach like ours to correctly infer the underlying IMT and AT distributions.

Protein production/degradation models cannot explain correlations

With the correct IMT and AT distributions inferred as outlined above, we then explored probable mechanistic origins of the lineage correlations in HCT116 cells. Previous work has suggested that cell-to-cell heterogeneity due to the stochastic production and degradation of proteins can influence cell fates and explain the correlation in IMT and AT in closely related cells25. We therefore sought to investigate whether such models would also be able to recapitulate the cousin-mother inequality observed in our data prior to cisplatin treatment (Fig. 1c, e). To compute lineage correlations, we added the additional capability of tracking lineages to our simulation framework using directed graphs (Supplementary section 6). In this framework, each vertex in the graph represents a unique cell and directed edges indicate a mother-daughter relationship. We kept track of the birth time and division time of each cell by assigning attributes to each vertex (Supplementary section 6). It has previously been shown that the level of a protein like CDK2 (or the ratio of the levels of two proteins like Cyclin D1 and p21) inherited by daughter cells at the mother’s division determines the chance of cell cycle progression versus quiescence27,28. To mimic this phenomenon, we generated stochastic trajectories of one protein (called Protein X) or two independent proteins (Proteins X and Y) within each single cell of our simulated lineage trees (Supplementary section 6). The level of Protein X (or the ratio of X and Y) in the mother cell that is passed on to the daughters sets the hazard function for division in our model (see Supplementary section 6 for details). As the level of Protein X at the time the mother divides increases, the probability of longer division times increases for the two daughter cells. As the level of Protein X decreases in the mother, there is an increased probability of shorter divisions for the daughters. In the case of two proteins controlling cell fate, the daughters are more likely to divide slower or faster depending on the magnitude of the ratio of their levels, X/Y. Within this general framework, we investigated a variety of protein production and degradation rates to mimic the fact that different proteins have varying “memory” levels and lose correlation at different timescales34 (Supplementary section 6).

We found that none of these models were able to generate higher cousin correlations than mother–daughter correlations. Figure 4 shows the correlations obtained for the Protein X only scenario, while the results for the Protein X and Protein Y models can be found in Supplementary Figure 7. As shown in Fig. 4a, when Protein X levels vary widely over time and lose memory of the initial level rapidly, the cousin correlation is less than the mother–daughter correlation and almost equal to zero (Fig. 4b). On the other hand, when Protein X has strong memory of its original state because of very low production and degradation rates (Fig. 4c), not only is the cousin correlation lower than the mother–daughter correlation, but the latter also becomes very strongly positive (Fig. 4d), which does not recapitulate the near zero mother–daughter correlation observed in our data. Similar results were found for the two-protein case, as shown in Supplementary Figure 7.

In summary, we found that simple models of stochastic production/degradation of proteins and their inheritance across cellular generations, representing our current understanding of cell cycle control mechanisms, cannot explain our observed correlation structures in the HCT116 cell line.

Unified theory with circadian gating explains correlations

The HCT116 cell line was shown to exhibit strong circadian oscillations with a period of 24 h35, and previous experiments suggested circadian control of both the cell cycle36,37,38 and cell death39,40 pathways. Motivated by these experimental observations and studies linking circadian gating to lineage correlations in IMT12,30, we developed a novel unified theory for explaining the observed correlation structures in HCT116 cells before and after cisplatin dosing. Since we had previously found that approximately 8% of HCT116 cells died over a period of 72 h in the absence of cisplatin29, we introduced the added dimension of cell death to our simulations (Fig. 5a) and found that while maintaining the correct IMT distribution (Fig. 5b), the origin of the correlations in the absence of drug cannot be ascribed to stochastic competition of fates alone (Fig. 5c). Next, based on our data suggesting that the cellular state inherited by a cell from its mother plays a major role in the decision of apoptosis versus division (Fig. 2a, b), we devised a form of coupling of the circadian clock to the cell cycle and cell apoptosis pathways: both hazard functions of division and death of any cell are determined by the circadian phase at the time the cell was born from its mother. Mathematically this coupling was achieved by introducing the following general structure for the parameter μ of the EMG:

$$\mu = \mu _0 + A\sin \left( \Phi \right),$$
(2)

where Φ represents the clock phase at the time a particular cell was born, and μ0 and A are two free parameters. An example plot of μ as a function of Φ and hazard functions of three cells born at different phases of the clock is shown in Fig. 5d (see Supplementary Equation 18 and Supplementary section 6 for further details.). We modeled the circadian clock as a sinusoidal wave of period 24 h, corresponding to a clock phase ranging from 0 to 2π. For cells born between the π and 2π phases of the clock corresponding to the second half of the circadian day, the probability to divide or die at earlier ages is increased (pink dot and line in Fig. 5d represent the risk of division; similar curves describe the risk of death). For cells born during the remainder of the phases (0 to π–the first half of the day), the probability is decreased (yellow dot and line in Fig. 5d). These probabilities were again modeled using hazard functions (Fig. 5d, Supplementary sections 5 and 6). This method of coupling the circadian clock to the cell cycle and cell death pathways via the hazard function is the defining aspect of our model, since it allows us to maintain the correct IMT and AT distributions as inferred from the data. The branching process model with this added gating mechanism was able to quantitatively reproduce the lineage correlations and the cousin–mother inequality observed in the pre-cisplatin part of the experiment (within 95% confidence intervals, Fig. 5e, f). Crucially, this model also reproduces the experimentally observed IMT distribution (Fig. 5g) and requires just one free parameter to recapitulate the correlation structures in addition to the three parameters required to characterize the IMT distribution (Supplementary section 6). Note that our results are robust to small phase differences between mother and daughters at the time of division36,41 (Supplementary section 6, Supplementary Figure 8). Furthermore, our model does not require the circadian clock of all cells to be synchronized (Supplementary section 6, Supplementary Figure 9)

.

The clock-driven correlations, as described above, were obtained by assuming a period of 24 h for the oscillations that couple to the cell cycle. We next investigated whether our model would be able to reproduce the correlation structure with other oscillation periods, since oscillatory processes distinct from the circadian clock have also been suggested to affect cellular proliferation42. To this end, we varied the oscillation period in our simulations, choosing the tunable parameters in a way that reproduced the sister correlations and IMT distribution observed in the data (Supplementary section 6; parameters are provided in Supplementary Table 7). Interestingly, we found that only certain multiples of ~12 h time-periods (approximately 12, 24, 48 h; not 36) could reproduce the experimentally observed correlation structure. For all other periods tested (for example 3.5, 6, 14, and 18.5 h), either one of two problems arose: (1) the mother–daughter correlation became strongly positive, for example with 14 and 18.5 h periods (Fig. 6 a, b and Supplementary Figure 10) or (2) at very small time periods like 3.5 h, the cousin correlations reduced to almost zero (Fig. 6c, d). An intuitive explanation for these observations is provided in Fig. 6 a, c. The mother–daughter correlation is set by the interplay between the variable cell cycle lengths and the period of oscillations of the clock. The HCT116 cell line has an approximately 16 h average cell division time. As shown in Fig. 6a, this cell cycle time along with an 18.5 h oscillation period would be expected to generate a strongly positive mother–daughter correlation as daughters are born in a similar part of the circadian cycle as their mothers. On the other hand, when the oscillator frequency is high (time period ~ 3.5 h), the heterogeneity in the cell division times will result in cousins being born at randomly different phases of the oscillator, thereby leading to negligible cousin correlations (Fig. 6c). These intuitive expectations are backed up by our simulation results which incorporate the correct heterogeneity in cell division times, and hence suggest that the circadian clock with a 24 h time period is likely to have generated the observed correlation structure. Ultradian oscillations, which have typical periods of ~2–4 h42 and are common in mammalian cells, are therefore unlikely driving the observed correlations.

We then investigated whether the circadian model could also explain the data arising from cisplatin-treated cells, where there is a significant amount of cell death and the drug induces a large extent of heterogeneity in the cell division and death times (Fig. 3d, e). We used the inferred IMT and AT distributions from our statistical model (Fig. 3d, e; parameters in Supplementary Table 5) as inputs to the birth-death process simulations. Similar to the pre-cisplatin scenario, we first investigated a null model with no coupling to the circadian clock (Fig. 7a, c) and subsequently studied a model with coupling of only the cell cycle to the circadian clock. We found that while the latter model was capable of generating high correlations in division times, it was not able to explain the experimentally observed magnitudes of correlations among the apoptosis times of either sister or cousin pairs (Supplementary Figure 11). This observation is interesting especially in light of previous work suggesting that correlated IMT of sisters can induce correlations among times to discordant fates of sister cells22,43. Our analysis predicts that even if the IMT of sisters are correlated due to circadian gating of the cell cycle, stochastic competition of fates alone cannot induce sufficiently large correlations in apoptosis times of sister or cousin cells.

A model with the circadian clock coupled to both cell cycle and cell death pathways, however, was able to recapitulate the high correlations in both IMT and AT (Fig. 7b, d). Note that our model predicts that the sister and cousin correlations in IMT after cisplatin addition are smaller than their values pre-cisplatin (Fig. 5f vs. 7b), similar to observations made from the data (Supplementary Figure 1 versus Fig. 1d, e). This decrease in correlations is due to the increased heterogeneity in IMT induced by cisplatin and highlights the importance of accounting for the correct level of variability in cell division (or apoptosis) times. A mechanistic model of lineage correlations therefore must be able to simultaneously account for these heterogeneities. Indeed, our circadian-gating model explains not only the correlation structures (Fig. 7b, d) but also the entire post-competition IMT and AT distributions (Supplementary Figure 12). Finally, to determine if our computational framework can also recapitulate the correlated fates of sisters (Fig. 2b), we introduced correlated random numbers to decide cell fate (Supplementary section 6). We found that this final computational model correctly predicts similarities in sister cell fates (Supplementary Figure 13).

Since the above results suggest that gating of both cell cycle and apoptosis pathways are simultaneously required, we explored the consequences of introducing a phase difference (Δφ) in the gating of the two pathways. When a daughter cell is born, the phase of the circadian clock at that moment could cause both the risks of eventual division or death to increase or decrease in sync (in phase, Δφ= 0). We found that this scenario was able to recapitulate the observed correlations in Fig. 7b, d. However, the circadian phase at the time of cell birth could also in principle increase the chance of division while decreasing that of death, and vice versa (out of phase, Δφ=π). A representation of the risks of division and death for these two extreme scenarios, Δφ= 0 and Δφ=π, are shown in Fig. 7e. We found that, as Δφ tends towards π (completely out of phase), the IMT correlations between sisters decrease to 0 or even negative values (Fig. 7f), thereby not recapitulating the observed data. These results suggest that the cell cycle and cell death pathways must be gated approximately in phase in the HCT116 cells treated with cisplatin.

Overall, our unified theory provides a comprehensive explanation of the correlation structures in intermitotic and apoptosis times, suggesting that the circadian phase passed on from mother to daughter cells during mitosis controls the chance of eventual division or death of the daughter.

Discussion

Distributions of cell division and apoptosis times along with lineage correlations among cells are signatures of underlying cellular processes, and deciphering their origins would provide a deep understanding of the fundamental principles of cell cycle control in both normal and cancer cells. However, comprehensive theories that account for both signatures do not currently exist. In addition, correct estimates of the extent of heterogeneity in cell division and death times, which are central to the understanding of both signatures, are difficult to obtain directly from experiments. Existing techniques for inferring these heterogeneities do not account for strong biases that arise from competition among various cellular fates.

To overcome these limitations, we developed a live cell imaging system to track cellular lineages both before and after drug dosing. Using this system, we uncovered complex correlation structures in the times to fate among closely related lineages both before and after chemotherapy administration. In addition, we found that correlations in cell fates and p53 dynamics in response to cisplatin treatment were highest in sister cells, decreasing with time and number of divisions separating cells, suggesting that a cellular state inherited by daughter cells at the time of their mother’s mitosis determines their likelihood of apoptosis. Although in different contexts, conceptually similar conclusions have previously been drawn in the case of TRAIL-induced apoptosis24 and in the cellular decision of proliferation versus quiescence27,28. Together, these results suggest the presence of a heritable cellular state, which determines the probabilities of eventual fate outcomes at the beginning of a cell’s lifetime.

We formalized these findings by creating a computational modeling framework in which the heritable cellular state is under circadian control. This approach provides a unified explanation to the entire set of correlation structures in cell division and apoptosis times both before and after treatment. Importantly, we showed that the magnitude of these correlations depends on the extent of heterogeneity in cell division and apoptosis times, and provide a new method to correctly infer their distributions. Our method, unlike previous approaches that treat cell division and death independently20, can be applied to any cell line treated with arbitrary drug concentrations. There is significant experimental evidence suggesting the control of both cell cycle and apoptosis pathways by the circadian clock36,37,38,39,40, thereby providing support to our modeling approach. Indeed, we demonstrated that our model was able to recapitulate the correlation structures of inter-mitotic times only for a few oscillation periods that are approximately multiples of 12 h, including 24 h (Fig. 6 and Supplementary Figure 10). Ultradian oscillations with typical periods of ~2–4 h42 are commonly observed in mammalian cells and are unlikely to drive the observed correlations. We cannot rule out some of the higher multiples of 12 h as potential time-periods of the oscillations, likely due to the 95% confidence intervals on the measured correlations being fairly large in our data.

Our circadian gating model is not mutually exclusive from previous protein production/degradation models that have been proposed specifically to explain correlations of sisters and cousins in drug-induced apoptosis times24,26. Indeed, since the circadian phase is likely to be passed on from mother to daughter cells via the fluctuating levels of proteins, our model conceptually encompasses the previously proposed mechanisms of generating correlations. While our theory in its current form does not explicitly model circadian protein concentrations and their oscillations, this information is implicitly incorporated in the varying circadian phase of our approach. However, we demonstrate that stochastic protein production and degradation alone cannot give rise to the entirety of the correlation structures, in particular the cousin-mother inequality in IMT (Fig. 4 and Supplementary Figure 7). Since by definition a cousin-mother inequality does not exist for AT, we cannot currently rule out the possibility that correlations in AT of related cells are due to stochastic production/degradation of non-oscillatory proteins. Finally, an advantage of our circadian gating model over previous work12,30 is that it relies on the birth-death process representation of cellular proliferation. A natural description of asexual reproduction where a single cell divides into two or dies after a stochastic waiting period, the birth–death process is widely used in contexts as diverse as the somatic evolution of cancer, bacterial dynamics, and genome evolution44,45,46. This model allows us to not only recapitulate the correlations observed in single cell data, but also the correct shapes of the highly variable division and death time distributions both before and after drug dosing. Our model is the first to explain such a diverse array of single cell results within one unified framework. It may, however, be possible to envision other more complex models that could explain all lineage correlations without invoking oscillatory mechanisms.

Our model generates exciting predictions for future experimental validation: we predict that both the IMT and AT should become less variable and less correlated if clock proteins are stabilized. This prediction may be directly tested by the addition of drugs like KL001, which have been shown to maintain the CRY protein at high levels throughout a circadian period47. In a recent study, deleting the clock genes in cyanobacteria led to a narrowing of the IMT distribution30, suggesting an important role of circadian gating in establishing the variation in cell division times. It would also be interesting to test our predictions in embryonic stem (ES) cells, which have been shown to develop the circadian clock only in later stages of differentiation as they lose their pluripotency48. Comparing correlation structures between ES cells in early versus late stages of differentiation could provide important insights into the consequences of circadian gating on cellular fates.

Methods

Cell culture and cell lines

HCT116 cells were obtained from ATCC and grown in McCoy’s with 10% FBS, 100 µg/ml penicillin, 0.25 µg/ml streptomycin and 85 µg/ml Amphotericin. For lineage tracking we used a previously established HCT116 p53-VKI clonal cell line where one allele of the TP53 gene is tagged at the endogenous locus. A lentiviral H2B-ECFP reporter was used to track cells over time.

Live-cell microscopy

To obtain cell lineages we plated approximately 5000 HCT116 p53-VKI H2B-CFP cells to poly-d-lysine coated glass bottom dishes with No. 1.5 thickness (MatTek corporation, P35GC-1.5–10-C) in McCoy’s media with 10% FBS. Cells were incubated at 37 °C and 5% CO2 for 72 h to allow cells to attach to the dishes. We then replaced the media with RPMI media lacking phenol red and riboflavin (imaging media) to reduce background fluorescence. Cells were imaged for 50 h in unstressed conditions to establish cell lineages. After 50 h, the media was replaced with imaging media containing 12.5 µM cisplatin and imaged for an additional 72 h to measure p53 dynamics and cell fate. Live cell microscopy was performed in a Nikon Eclipse Ti-E microscope in an enclosure to keep cells at 37 °C, 5% CO2 and maintain humidity. Images were captured using MetaMorph software every 30 min. We used the following filter sets: Venus—500/20, 515, 520 nM (excitation, beam splitter, emission filter); ECFP—436/20, 455, 480/40 nm. All filters were obtained from Chroma.

Data analysis

For cell tracking and image analysis, we used custom made software for Matlab (MathWorks) that allows the user to manually track cell lineages over time using both the H2B-ECFP nuclear marker and Phase contrast images to ensure faithful tracking49. Cell identities that were ambiguous were discarded to ensure the reliability of cell lineages. The p53-Venus traces were extracted from background subtracted images in Matlab and are the average of 9 pixels in the center of each nuclei. Cell death and division was identified by morphology in the phase channel, apoptotic cells detach from the glass while the membrane blebs out. Morphology of the localization of the H2B marker also allows identification of cell death and division, with cell death shown by a loss of the normal round nuclear shape and division shown by condensation of the chromatin to mitotic chromosomes and then separation to two separate nuclei. To determine whether cell fate was correlated in related cells a χ2 test was used to compare the expected and observed portion of cells that share the same fate. For calculations of significance for R-values in Fig. 2 and Supplemental Fig. 2, we compared measured R-values of related cells to the distribution of 10,000 R-values measured from randomly paired cells of equal size.

Mathematical models and simulations

The mathematical models and computational algorithms were written in R, version 3.4.0, and Wolfram Mathematica, version 11.0. These are described in detail in the Supplementary Information.

Code availability

All code used for the computational modeling will be made available on request to the corresponding authors. The code for lineage tracking and quantifying single cell data can be downloaded at: github.com/balvahal/p53CinemaManual.

Data availability

The lineage data generated in this study and used for all the analysis is available in Supplementary Data 1 along with an explanation of the data structure in Supplementary Data 2.

References

1. 1.

Hartwell, L. H., Culotti, J., Pringle, J. R. & Reid, B. J. Genetic control of the cell division cycle in yeast. Science 183, 46–51 (1974).

2. 2.

Nurse, P. Genetic control of cell size at cell division in yeast. Nature 256, 547–551 (1975).

3. 3.

Hartwell, L. H. & Weinert, T. A. Checkpoints: controls that ensure the order of cell cycle events. Science 246, 629–634 (1989).

4. 4.

Milestones in cell division. Nat. Cell Biol. 3, E265–E265 (2001).

5. 5.

Cardone, L. & Sassone-Corsi, P. Timing the cell cycle. Nat. Cell Biol. 5, 859–861 (2003).

6. 6.

Hunt, T. & Sassone-Corsi, P. Riding tandem: circadian clocks and the cell cycle. Cell 129, 461–464 (2007).

7. 7.

Masri, S., Cervantes, M. & Sassone-Corsi, P. The circadian clock and cell cycle: interconnected biological circuits. Curr. Opin. Cell Biol. 25, 730–734 (2013).

8. 8.

Fu, L. & Lee, C. C. The circadian clock: pacemaker and tumour suppressor. Nat. Rev. Cancer 3, 350–361 (2003).

9. 9.

Sahar, S. & Sassone-Corsi, P. Metabolism and cancer: the circadian clock connection. Nat. Rev. Cancer 9, 886–896 (2009).

10. 10.

Kiessling, S. et al. Enhancing circadian clock function in cancer cells inhibits tumor growth. BMC Biol. 15, 13 (2017).

11. 11.

Lévi, F. Circadian chronotherapy for human cancers. Lancet Oncol. 2, 307–315 (2001).

12. 12.

Sandler, O. et al. Lineage correlations of single cell division time as a probe of cell-cycle dynamics. Nature 519, 468–471 (2015).

13. 13.

Froese, G. The distribution and interdependence of generation times of HeLa cells. Exp. Cell Res. 35, 415–419 (1964).

14. 14.

Koch, A. L. & Schaechter, M. A model for statistics of the cell division process. J. Gen. Microbiol. 29, 435–454 (1962).

15. 15.

Grasman, J. A deterministic model of the cell cycle. Bull. Math. Biol. 52, 535–547 (1990).

16. 16.

Staudte, R. G., Huggins, R. M., Zhang, J., Axelrod, D. E. & Kimmel, M. Estimating clonal heterogeneity and interexperiment variability with the bifurcating autoregressive model for cell lineage data. Math. Biosci. 143, 103–121 (1997).

17. 17.

Yu, F. B. et al. Long-term microfluidic tracking of coccoid cyanobacterial cells reveals robust control of division timing. BMC Biol. 15, 11 (2017).

18. 18.

Strasser, M. K. et al. Lineage marker synchrony in hematopoietic genealogies refutes the PU.1/GATA1 toggle switch paradigm. Nat. Commun. 9, 2697 (2018).

19. 19.

Mosheiff, N. et al. Inheritance of cell-cycle duration in the presence of periodic forcing. Phys. Rev. X 8, 021035 (2018).

20. 20.

Tyson, D. R., Garbett, S. P., Frick, P. L. & Quaranta, V. Fractional proliferation: a method to deconvolve cell population dynamics from single-cell data. Nat. Methods 9, 923–928 (2012).

21. 21.

Golubev, A. Exponentially modified Gaussian (EMG) relevance to distributions related to cell proliferation and differentiation. J. Theor. Biol. 262, 257–266 (2010).

22. 22.

Duffy, K. R. et al. Activation-induced B cell fates are selected by intracellular stochastic competition. Science 335, 338–341 (2012).

23. 23.

Hawkins, E. D., Markham, J. F., McGuinness, L. P. & Hodgkin, P. D. A single-cell pedigree analysis of alternative stochastic lymphocyte fates. Proc. Natl Acad. Sci. 106, 13457–13462 (2009).

24. 24.

Spencer, S. L., Gaudet, S., Albeck, J. G., Burke, J. M. & Sorger, P. K. Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis. Nature 459, 428–432 (2009).

25. 25.

Bhola, P. D. & Simon, S. M. Determinism and divergence of apoptosis susceptibility in mammalian cells. J. Cell. Sci. 122, 4296–4302 (2009).

26. 26.

Bertaux, F., Stoma, S., Drasdo, D. & Batt, G. Modeling dynamics of cell-to-cell variability in TRAIL-induced apoptosis explains fractional killing and predicts reversible resistance. PLoS Comput. Biol. 10, e1003893 (2014).

27. 27.

Spencer, S. L. et al. The proliferation-quiescence decision is controlled by a bifurcation in CDK2 activity at mitotic exit. Cell 155, 369–383 (2013).

28. 28.

Yang, H. W., Chung, M., Kudo, T. & Meyer, T. Competing memories of mitogen and p53 signalling control cell-cycle entry. Nature 549, 404–408 (2017).

29. 29.

Paek, A. L., Liu, J. C., Loewer, A., Forrester, W. C. & Lahav, G. Cell-to-cell variation in p53 dynamics leads to fractional killing. Cell 165, 631–642 (2016).

30. 30.

Mosheiff, N. et al. Inheritance of cell-cycle duration in the presence of periodic forcing. Phys. Rev. X 8, 021035 (2018).

31. 31.

Nelsen, R. B. An Introduction to Copulas (Springer-Verlag, New York, 2006).

32. 32.

Bouyé, E., Durrleman, V., Nikeghbali, A., Riboulet, G. & Roncalli, T. Copulas for Finance—A Reading Guide and Some Applications (Social Science Research Network, 2000).

33. 33.

Stukalin, E. B., Aifuwa, I., Kim, J. S., Wirtz, D. & Sun, S. X. Age-dependent stochastic models for understanding population fluctuations in continuously cultured cells. J. R. Soc. Interface 10, 20130325 (2013).

34. 34.

Sigal, A. et al. Variability and memory of protein levels in human cells. Nature 444, 643–646 (2006).

35. 35.

Relógio, A. et al. Ras-mediated deregulation of the circadian clock in cancer. PLoS Genet. 10, e1004338 (2014).

36. 36.

Nagoshi, E. et al. Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells. Cell 119, 693–705 (2004).

37. 37.

Lévi, F., Filipski, E., Iurisci, I., Li, X. M. & Innominato, P. Cross-talks between circadian timing system and cell division cycle determine cancer biology and therapeutics. Cold Spring Harb. Symp. Quant. Biol. 72, 465–475 (2007).

38. 38.

Yang, Q., Pando, B. F., Dong, G., Golden, S. S. & van Oudenaarden, A. Circadian gating of the cell cycle revealed in single cyanobacterial cells. Science 327, 1522–1526 (2010).

39. 39.

Sancar, A. et al. Circadian clock, cancer, and chemotherapy. Biochemistry 54, 110–123 (2015).

40. 40.

Gotoh, T. et al. The circadian factor Period 2 modulates p53 stability and transcriptional activity in unstressed cells. Mol. Biol. Cell 25, 3081–3093 (2014).

41. 41.

Bieler, J. et al. Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells. Mol. Syst. Biol. 10, 739 (2014).

42. 42.

Isomura, A. & Kageyama, R. Ultradian oscillations and pulses: coordinating cellular responses and cell fate decisions. Development 141, 3627–3636 (2014).

43. 43.

Duffy, K. R. & Hodgkin, P. D. Intracellular competition for fates in the immune system. Trends Cell Biol. 22, 457–464 (2012).

44. 44.

Chakrabarti, S. & Michor, F. Pharmacokinetics and drug interactions determine optimum combination strategies in computational models of cancer evolution. Cancer Res. 77, 3908–3921 (2017).

45. 45.

Nee, S. Birth-death models in macroevolution. Annu. Rev. Ecol. Evol. Syst. 37, 1–17 (2006).

46. 46.

Novozhilov, A. S., Karev, G. P. & Koonin, E. V. Biological applications of the theory of birth-and-death processes. Brief. Bioinform. 7, 70-85 (2006).

47. 47.

Hirota, T. et al. Identification of small molecule activators of cryptochrome. Science 337, 1094–1097 (2012).

48. 48.

Yagita, K. et al. Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro. Proc. Natl Acad. Sci. 107, 3846–3851 (2010).

49. 49.

Reyes, J. et al. Fluctuations in p53 signaling allow escape from cell-cycle arrest. Mol. Cell 71, 581–591 (2018).

Acknowledgements

We would like to thank Mukund Thattai, Jacob Stewart-Ornstein and Bill Forrester for interesting discussions and comments. We also thank the Nikon Imaging Center at Harvard Medical School for help with microscopy. This research was supported by National Institute of Health grants GM083303 and GM116864, and the Ludwig Center at Harvard. A.L.P. was supported by an American Cancer Society New England Division—Ellison Foundation Postdoctoral Fellowship. J.R. received support from CONACyT/Fundacion Mexico en Harvard and a Harvard Graduate Merit Fellowship. The authors gratefully acknowledge support of the Dana-Farber Cancer Institute Physical Sciences Oncology Center (NCI grant U54CA193461).

Author information

Authors

Contributions

S.C., A.L.P., G.L., and F.M. designed and organized the study. A.L.P. performed the experiments. S.C., A.L.P., J.R., and K.L. analyzed the data. S.C. wrote and implemented all algorithms and mathematical models. S.C., A.L.P., and K.L. created figures. S.C., A.L.P. G.L., and F.M. wrote the manuscript.

Corresponding authors

Correspondence to Galit Lahav or Franziska Michor.

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Chakrabarti, S., Paek, A.L., Reyes, J. et al. Hidden heterogeneity and circadian-controlled cell fate inferred from single cell lineages. Nat Commun 9, 5372 (2018). https://doi.org/10.1038/s41467-018-07788-5

• Accepted:

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• The role of the circadian clock in cancer hallmark acquisition and immune-based cancer therapeutics

• Elizabeth Cash
• , Sandra Sephton
• , Cassandra Woolley
• , Attia M. Elbehi
• , Anu R. I.
• , Bene Ekine-Afolabi
•  & Victor C. Kok

Journal of Experimental & Clinical Cancer Research (2021)