Inhibition of cell division is an active response to DNA damage that enables cells to maintain genome integrity. However, how DNA damage arrests the plant cell cycle is largely unknown. Here, we show that the repressor-type R1R2R3-Myb transcription factors (Rep-MYBs), which suppress G2/M-specific genes, are required to inhibit cell division in response to DNA damage. Knockout mutants are resistant to agents that cause DNA double-strand breaks and replication stress. Cyclin-dependent kinases (CDKs) can phosphorylate Rep-MYBs in vitro and are involved in their proteasomal degradation. DNA damage reduces CDK activities and causes accumulation of Rep-MYBs and cytological changes consistent with cell cycle arrest. Our results suggest that CDK suppressors such as CDK inhibitors are not sufficient to arrest the cell cycle in response to DNA damage but that Rep-MYB-dependent repression of G2/M-specific genes is crucial, indicating an essential function for Rep-MYBs in the DNA damage response.
Genotoxic stress threatens genome integrity and is unavoidable for every organism. Various kinds of environmental stress as well as cellular processes (e.g., DNA replication, metabolism) can cause DNA damage; in plants, high boron or aluminium levels in soil, pathogen attack and stress-induced reactive oxygen species are known to damage genomic DNA1,2,3,4. To prevent the transmission of damaged DNA to daughter cells, cell cycle progression is delayed or arrested before the cell enters DNA replication or mitosis. In animals, the DNA damage checkpoint is triggered by the sensor kinases ATM (ataxia telangiectasia mutated) and ATR (ATM-related and Rad3-related); ATM senses DNA double-strand breaks (DSBs), while ATR is primarily activated by replication stress and single-strand breaks (SSBs)5, 6. Downstream of these kinases, checkpoint-1 and checkpoint-2 kinases (CHK1 and CHK2) phosphorylate and stabilize the transcription factor p53, which then induces expression of the CDK inhibitor p21 and reduces the activity of cyclin-dependent kinases (CDKs)7, 8. Plants also possess functional ATM and ATR9, 10, but these downstream regulators are all missing in plants; instead, suppressor of gamma response 1 (SOG1) has been identified in Arabidopsis as a plant-specific transcription factor that controls the DNA damage response11. Recent reports have shown that ATM and ATR directly phosphorylate and activate SOG112, 13.
DSBs inhibit plant cell division, but also induce an early transition from cell division to endoreplication, a repeated cycle of DNA replication without mitosis or cytokinesis14. Endoreplication is triggered by blocking the G2-to-M progression; indeed, in Arabidopsis cultured cells, DSBs arrest the cell cycle at G2 before provoking endoreplication14. Another typical response to DSBs is cell death around the stem cell region of shoot and root meristems15, 16. Both endoreplication and cell death occur in an ATM/ATR- and SOG1-dependent manner, implying a programmed response to DNA damage. We previously reported that DSBs increase the expression of CDK suppressors, such as CDK inhibitors, and downregulate cyclin A (CYCA) and B (CYCB) genes, which are essential for CDK activation at G2/M14. However, it remains unknown whether such a dramatic change in expression of cell cycle regulators is a cause or a consequence of G2 arrest.
In plants, many G2/M-specific genes, including those for mitotic cyclins, kinesin-like proteins and the cytokinesis-specific syntaxin KNOLLE, are controlled by three Myb repeat-containing transcription factors, called R1R2R3-Myb (MYB3R)17. The Arabidopsis genome has five MYB3R genes, MYB3R1 to MYB3R5. MYB3R1 and MYB3R4 act as transcriptional activators, while MYB3R3 and MYB3R5 are transcriptional repressors18,19,20. MYB3R1 exhibits a repressor function as well; therefore, MYB3R1 and MYB3R4 are known as the activator-type (Act-MYBs), and MYB3R1, MYB3R3 and MYB3R5 as the repressor-type (Rep-MYBs)20. Although both Act- and Rep-MYBs bind to the cis-acting MSA element on target gene promoters, Rep-MYBs repress transcription along the cell cycle except G2/M in proliferating cells, and suppress G2/M-specific genes in post-mitotic cells during organ development20.
In this study, we show that Rep-MYBs play a crucial role in inhibiting cell division under DNA damage conditions. We propose a model whereby a reduction in CDK activity leads to accumulation of Rep-MYBs, which in turn suppresses mitotic genes, representing an elaborate mechanism for stress-induced cell cycle arrest.
myb3r3 and myb3r5 mutants are tolerant to genotoxic stress
Since our previous observation demonstrated that DSBs trigger G2 arrest in Arabidopsis cultured cells14, we first examined the involvement of MYB3Rs in the DNA damage response. Among the five MYB3Rs, MYB3R2 is only distantly related to the other MYB3Rs, and MYB3R1 acts as both an activator and a repressor, and exhibits a supplementary function; indeed, in the myb3r1 knockout mutant, G2/M-specific genes are expressed at the same levels as they are in wild-type (WT)20. Therefore, we excluded these two MYB3Rs from further analyses, and observed T-DNA insertion mutants of MYB3R3, MYB3R4 and MYB3R5. Seedlings grown on Murashige and Skoog (MS) medium for 5 days were transferred to medium with or without 2 µM zeocin, an inducer of DSBs21, and grown for a further 6 days (Fig. 1a). myb3r3-1 and myb3r5-1 (hereafter called myb3r3 and myb3r5, respectively), which completely lack normal transcripts20, displayed higher tolerance to zeocin than WT (Fig. 1b). Faster root growth was also observed at lower (1 µM) and higher (4 µM) zeocin concentrations (Supplementary Fig. 1). Similar results were obtained with two other alleles, myb3r3-2 and myb3r5-2, as shown in Supplementary Fig. 2. The myb3r3 myb3r5 double mutant was tolerant to zeocin to an extent similar to each single mutant (Supplementary Fig. 3a), implying that both MYB3R3 and MYB3R5 are essential for root growth arrest. Zeocin tolerance was comparable between myb3r3, sog1-1 and the myb3r3 sog1-1 double mutant (Supplementary Fig. 3b), suggesting that Rep-MYBs function in SOG1-mediated inhibition of root growth.
myb3r4-2 (hereafter called myb3r4) has a T-DNA insertion 35 bp upstream of the start codon, and the MYB3R4 transcript level decreases to less than 3% of the WT level18. In this mutant, root growth was inhibited by zeocin to the same extent as in WT (Fig. 1b). To examine the possibility that residual MYB3R4 transcripts function in myb3r4, we used another allele, myb3r4-3, which carries two copies of T-DNA insertions and produces no MYB3R4 transcripts (Supplementary Fig. 4). Our data showed that roots exhibited the same sensitivity to zeocin as that in WT and myb3r4-2 (Supplementary Fig. 2), indicating that MYB3R4 is not involved in controlling root growth in response to DSBs.
Observation of the root tip showed that increasing zeocin concentrations resulted in weaker reduction of the meristem cell number in myb3r3 and myb3r5, but not in myb3r4, compared to WT (Fig. 1c and Supplementary Fig. 5). Moreover, propidium iodide-stained dead cells were observed in stem cells and stele precursor cells of WT and myb3r4 in the presence of 1 µM zeocin, while a higher concentration was required for myb3r3 and myb3r5 (Supplementary Fig. 5). These results suggest that Rep-MYBs participate in the inhibition of cell division and induction of stem cell death in response to DSBs.
Next, we tested the response of myb3r3 and myb3r5 to other DSB-inducing agents, namely bleomycin, bleocin, gamma irradiation and boron overload. These mutants were significantly more tolerant to all four treatments than WT (Supplementary Fig. 6). We then examined the response to hydroxyurea (HU) and aphidicolin, which inhibit DNA replication by decreasing the dNTP pool and by inhibiting DNA polymerase α, respectively22, 23. Although HU had a milder effect than aphidicolin, both treatments revealed tolerance in myb3r3 and myb3r5 (Supplementary Fig. 6). These results suggest that Rep-MYBs function in the response to replication stress as well as to DSBs.
To investigate whether Rep-MYBs are also involved in the control of cell division in the shoot apex, we irradiated Arabidopsis seeds with 200 or 400 Gy of gamma rays and grew the seedlings for 10 days in MS medium, followed by counting the number of true leaves. sog1-1 and lig4, the latter of which is defective in non-homologous end-joining, exhibited lower and higher sensitity to gamma irradiation, respectively, as reported previously (Supplementary Fig. 7)11, 24. myb3r3, myb3r5 and the myb3r3 myb3r5 double mutant produced more true leaves than WT at 400 Gy (Supplementary Fig. 7), suggesting that Rep-MYBs also function in DNA damage-induced inhibition of cell division in the shoot apex.
Rep-MYB proteins accumulate highly under DNA stress
To examine whether MYB3R expression responds to DNA damage, we conducted quantitative real-time PCR (qRT-PCR) using RNA from root tips. The transcript level of MYB3R4 decreased by about 70% within 1 h of zeocin treatment, while those of MYB3R3 and MYB3R5 did not change during the 12-h treatment (Supplementary Fig. 8). This result agrees with data obtained from zeocin-treated cultured cells14.
Since Rep-MYBs did not respond to zeocin at the mRNA level, we next observed protein accumulation of MYB3R3 using ProMYB3R3::MYB3R3-GFP, which carries the 1.3-kb promoter and the protein-coding region of MYB3R3. This reporter gene can complement the myb3r3 mutation, indicating that the MYB3R3-GFP fusion protein is functional20. GFP signals were very faint in the absence of zeocin, but became pronounced in the meristem after 12 h of zeocin treatment and thereafter (Fig. 2a). Immunoblot analysis of total protein from roots also showed marked accumulation of MYB3R3-GFP, dependent on zeocin concentration and treatment time (Fig. 2b). When the proteasome inhibitor MG132 was applied to the reporter line in the absence of zeocin, GFP fluorescence was again enhanced in the root tip, and dramatic accumulation of MYB3R3-GFP was observed by immunoblotting (Fig. 2c, d). Similar patterns of protein accumulation upon zeocin or MG132 treatment were also observed for MYB3R5, albeit to a lesser extent than MYB3R3 (Supplementary Fig. 9). These data suggest that Rep-MYBs are actively degraded via the ubiquitin-proteasome pathway under normal growth conditions, but accumulate to high levels in response to DNA damage.
We also examined protein accumulation of MYB3R4 using ProMYB3R4::MYB3R4-GFP. Since the MYB3R4-GFP fusion protein was barely detected by immunoblotting, we quantified GFP fluorescence in the root tip to estimate the protein level. The amount of MYB3R4-GFP was greatly reduced within 6 h of zeocin treatment (Supplementary Fig. 10a, b), correlating with a rapid decrease in the transcript level (Supplementary Fig. 8). Unlike Rep-MYBs, MG132 treatment did not change the protein level of MYB3R4-GFP under normal growth conditions (Supplementary Fig. 10c, d), suggesting that MYB3R4 is not under the control of proteasomal degradation.
DSBs induce cell cycle arrest in roots
Since Rep-MYBs are involved in repression of G2/M-specific genes20, it is possible that their accumulation causes cell cycle arrest. We therefore investigated cell cycle stages of root meristematic cells under DNA damage conditions. Flow cytometry analysis using root tip cells cannot distinguish mitotic 4C cells from endocycling 4C cells, which increase in response to DSBs14. Moreover, the G2/M-marker gene CYCB1;1 is responsive to DNA damage25; thus, we took another approach. Since nuclear DNA content, which increases during progression through S phase, is well correlated with nuclear size26, we measured the nuclear area of each meristematic cell to estimate cell cycle stages. A previous report showed that the incorporation of 5-ethynyl-2′-deoxyuridine (EdU) into DNA stains whole nuclei of early S-phase cells, while speckled signals are detected for late S-phase cells due to DNA replication of heterochromatic regions27. Thus, we used EdU staining to identify early and late S-phase cells (Fig. 3a). G1- and G2-phase cells were searched for using the dual-colour marker system Cytrap; as previously reported28, cells exhibiting both RFP and GFP fluorescence were identified as late G2-phase cells, and non-fluorescing cells as G1-phase cells (Fig. 3a). Our measurement of nuclear area at each cell cycle stage showed that it increased in the progression of G1, early S, late S and late G2 (Fig. 3b), reflecting the elevation of DNA content during replication.
When WT seedlings were treated with zeocin, the nuclear area increased significantly (Fig. 3c), suggesting cell cycle arrest during S/G2, but not G1. To further investigate cell cycle progression in the presence of zeocin, roots were treated with EdU for 15 min, and the number of EdU-labelled cells with mitotic figures was then counted. Our results showed that the percentage of EdU-labelled cells among those with mitotic figures was elevated in the absence of zeocin and reached a maximum after 6 h, while such an increase was delayed and suppressed after treatment with zeocin (Supplementary Fig. 11). This result suggests that DSBs inhibit G2 progression in root meristematic cells, thereby preventing their entry into M phase.
To examine whether Rep-MYB accumulation is associated with zeocin-induced cell cycle arrest, we then used the myb3r3 mutant harbouring ProMYB3R3::MYB3R3-GFP. When nuclei of GFP-expressing cells were observed in the absence of zeocin, the distribution of nuclear area was similar to that in early S-phase cells (Fig. 3b, Early S; Fig. 3d, Mock). This suggests that MYB3R3 protein preferentially accumulates around early S phase under normal growth conditions. We found that zeocin treatment increased the nuclear area of GFP-expressing cells in a time-dependent manner: the average nuclear area increased at 24 h and reached that of late G2-phase cells at 72 h (Fig. 3d). Although measuring the nuclear area alone cannot clearly identify cell cycle phases, this result suggests that zeocin-induced DSBs enhance MYB3R3 accumulation, thereby leading to cell cycle arrest.
Rep-MYB degradation is dependent on CDK activities
The next question is how Rep-MYB accumulation is controlled in response to DNA damage. Araki et al.29 demonstrated that NtmybA2, one of the tobacco Act-MYBs, is phosphorylated and activated by CDK. A transient transactivation assay showed that Arabidopsis MYB3R4 is activated by co-expression with CYCB1 18. Therefore, we speculated that Rep-MYBs are also under the control of CDK. To test this possibility, we first treated plants carrying ProMYB3R3::MYB3R3-GFP or ProMYB3R5::MYB3R5-GFP with the CDK-specific inhibitor roscovitine. We observed a marked increase in GFP signals and protein levels of MYB3R3- and MYB3R5-GFP after 24 h of roscovitine treatment (Fig. 2c, d and Supplementary Fig. 9). The same treatment did not change the transcript levels of MYB3R3 or MYB3R5 (Supplementary Fig. 12), indicating that Rep-MYB accumulation upon roscovitine treatment is controlled at the protein level. These results suggest that CDK activity is required for Rep-MYB degradation under normal growth conditions. We also examined the transcript and protein levels of MYB3R4 and found that they were elevated upon roscovitine treatment for some unknown reason (Supplementary Figs. 10c, d and 12). As described above, MYB3R4 expression is rapidly reduced by zeocin treatment, although DNA damage reduces CDK activity. This indicates the existence of a mechanism to actively downregulate MYB3R4 expression in response to DNA damage.
We then examined whether protein stability of MYB3R3 is affected by CDK activity or DNA damage by performing an in vitro assay according to a previously described method30. Histidine (His)-tagged MYB3R3 produced in Escherichia coli was incubated with total protein extracted from WT roots, and the remaining amount of His-MYB3R3 was estimated by immunoblotting using anti-His antibody. A reduction of His-MYB3R3 was observed after a 5-min incubation, and almost no protein was detected after 20 min (Fig. 4a). However, when MG132 or roscovitine was included in the reaction, His-MYB3R3 remained detectable after a 30-min incubation (Fig. 4a). When ATP was omitted from the reaction, His-MYB3R3 degradation was suppressed (Fig. 4a), suggesting that MYB3R3 phosphorylation and/or ATP-dependent ubiquitination are impaired, thus preventing proteasomal degradation. Next, we used protein extracts from zeocin-treated roots; His-MYB3R3 was still detected after a 20-min incubation, indicating slower degradation than in the non-treated control (Fig. 4b). This and the above results of GFP reporter lines suggest that proteasome-mediated degradation of MYB3R3 is dependent on CDK activities and is suppressed by DNA damage.
CDK phosphorylates the C-terminal region of MYB3R3
To examine whether Rep-MYBs are directly phosphorylated by CDK, we conducted an in vitro kinase assay. In plants, two types of CDKs control cell cycle progression: CDKA is an orthologue of mammalian CDK1 and yeast Cdc2/Cdc28, and CDKB is a plant-specific type with two subfamilies, CDKB1 and CDKB231. Each CDK was immunoprecipitated from Arabidopsis cultured cells using specific antibodies32,33,34, and subjected to kinase assay. As shown in Fig. 5a, all three CDKs phosphorylated His-MYB3R3 and His-MYB3R5, but not His-GFP. CDKA exhibited a higher kinase activity than CDKBs in our assay, but this may reflect variable avidity of CDK antibodies and/or variable amounts of active CDK-cyclin complexes in cultured cells. Nevertheless, our results indicate that both CDKA and CDKB can phosphorylate Rep-MYBs in vitro.
Next, to identify the phosphorylation sites, we dissected the MYB3R3 coding region into three fragments, F1, F2 and F3 (Fig. 5b). CDKA immunoprecipitated from cultured cells phosphorylated F3, but neither F1 nor F2 (Fig. 5c). We then conducted liquid chromatography−tandem mass spectrometry (LC−MS/MS). Active CDKA-cyclin D3 complexes were produced in E. coli 35, and subjected to a kinase reaction with GST-MYB3R3. We identified six phosphorylated residues, among which Ser394 (S394), Thr407 (T407) and Ser461 (S461) were located in the F3 fragment (Supplementary Fig. 13). We also detected phosphorylation of the corresponding residues (S412, T427 and S493) in MYB3R5 (Supplementary Fig. 14). Note that these residues belong to the consensus motif of CDK phosphorylation sites (S/T-P). To identify in vivo phosphorylation sites, we tried to overexpress MYB3R3 in Arabidopsis plants and in protoplasts of Arabidopsis and tobacco cultured cells. However, we were unable to obtain sufficient protein for MS analysis, probably due to the highly unstable nature of phosphorylated MYB3R3.
In vitro kinase assays using His-MYB3R3 with alanine substitutions showed that phosphorylation level did not decrease for S394A or T407A, and not markedly for S461A (Fig. 5d), indicating the presence of multiple phosphorylation sites. A dramatic reduction in phosphorylation level was observed for S394A/T407A, S394A/S461A and S394A/T407A/S461A, but not for T407A/S461A (Fig. 5d). These results suggest that S394 is the major phosphorylation site, while T407 and S461 are also targeted by CDK. The persistent phosphorylation level observed for S394A indicates higher phosphorylation at T407 and/or S461 in this mutated protein (Fig. 5d), suggesting complementary roles of these three residues in being targeted by CDK. Impaired protein degradation was observed in vitro for His-MYB3R3 with non-phosphorylatable alanine substitutions at all three sites (hereafter called MYB3R3AAA) (Fig. 4c), suggesting that CDK phosphorylation at these residues is indeed responsible for MYB3R3 degradation.
Phosphomimic MYB3R3 cannot suppress root growth
We then asked whether phosphorylation of Rep-MYBs is associated with the DNA damage response. As described above, GFP-fused MYB3R3 accumulates at a very low level in the root tip under non-stress conditions (Fig. 2c, d); however, MYB3R3AAA-GFP expressed under the MYB3R3 promoter in myb3r3 showed higher GFP fluorescence, and we observed less dramatic increases in the fluorescence levels following zeocin or MG132 treatment (Fig. 6a). This result was supported by immunoblotting using anti-GFP antibody (Fig. 6b), indicating that MYB3R3AAA-GFP accumulates highly even in the absence of DNA damage. We also introduced ProMYB3R3::MYB3R3DDD-GFP, which carries phosphomimic substitutions to aspartic acid at the three phosphorylation sites, into myb3r3. The level of MYB3R3DDD-GFP was very low in the absence of zeocin, and was not as highly elevated by zeocin treatment as that for ProMYB3R3::MYB3R3-GFP (Figs. 2c, d and 6a, b). This suggests that a decrease in phosphorylation of the three amino acids is necessary for DNA damage-induced protein accumulation of MYB3R3.
Kobayashi et al.20 reported that loss of Rep-MYBs caused enhanced organ growth and ectopic cell division. In agreement with those results, the myb3r3 mutant displayed slightly enhanced shoot growth (Fig. 6c). Whereas myb3r3 expressing MYB3R3DDD-GFP showed a similarly enhanced phenotype, expression of MYB3R3AAA-GFP suppressed shoot growth as compared to WT (Fig. 6c). Slower growth was also noted in myb3r3 roots harbouring ProMYB3R3::MYB3R3AAA-GFP (Fig. 6d). It is noteworthy that the average nuclear area of root meristematic cells expressing MYB3R3AAA-GFP was larger than of those expressing ProMYB3R3::MYB3R3-GFP (Fig. 3d). This suggests that a higher accumulation of MYB3R3AAA-GFP delayed cell cycle progression, thereby slowing down shoot and root growth.
In the presence of 2 µM zeocin, myb3r3 expressing MYB3R3-GFP or MYB3R3AAA-GFP showed root growth inhibition to a similar extent to WT, while expression of MYB3R3DDD-GFP did not rescue the mutant phenotype, but rather led to tolerance to zeocin as observed in myb3r3 (Fig. 6d, e). As described below, alanine substitutions at the three phosphorylation sites did not impair the ability of MYB3R3 to bind to target promoters or to repress target genes, suggesting that the three residues are not essential for protein function; rather, they control protein accumulation. Therefore, it is likely that the failure of MYB3R3DDD-GFP to rescue the mutant phenotype was due to a protein level that was too low to suppress G2/M-specific genes.
In myb3r3 and myb3r5, the CDK inhibitor genes SMR5 and SMR7, and the Act-MYB gene MYB3R4, were rapidly upregulated and downregulated by zeocin, respectively, as in WT (Supplementary Fig. 15). A previous analysis of global transcription and our qRT-PCR data for MYB3R4 indicate that these transcriptional responses are dependent on SOG111 (Supplementary Fig. 16). This indicates that, in myb3r3 and myb3r5, an initial reduction in CDK activity normally occurs by upregulation and downregulation of genes for CDK inhibitors and Act-MYB, respectively, in a SOG1-dependent manner, but it is not sufficient for growth arrest. This supports the above-mentioned idea that Rep-MYBs, which avoid CDK phosphorylation and accumulate highly under DNA stress, play an indispensable role in inhibiting cell division and organ growth.
Rep-MYBs suppress G2/M-specific genes upon DNA damage
We next examined whether DNA damage affects the binding of MYB3R3 to promoters of G2/M-specific genes. We focused on four genes identified as MYB3R3 targets: KNOLLE, CYCB1;2, EDE1 and IMK2 20. Chromatin immunoprecipitation (ChIP) using ProMYB3R3::MYB3R3-GFP seedlings showed that the promoter fragments of all four genes were highly enriched by anti-GFP antibody (Fig. 7a). The promoter of CDKA;1, whose expression is not specific to G2/M31, did not show any enrichment, indicating the specificity of ChIP (Fig. 7a). When seedlings were treated with 2 µM zeocin for 24 h, MYB3R3 binding was significantly enhanced on the promoters of KNOLLE and CYCB1;2, but not EDE1 or IMK2, relative to the non-treated control (Fig. 7b). We also tested other MYB3R3 target genes; MYB3R3 binding to the promoters of PAKRP1, EHD2 and PLE/MAP65-3 was highly elevated by zeocin treatment, while a slight or no increase was observed for those of AUR2, PAKRP2 and CDC20.1 (Supplementary Fig. 17). These results indicate that MYB3R3, highly accumulated under DNA damage conditions, efficiently binds to the promoters of some of its target genes. The binding of MYB3R3 to the KNOLLE and CYCB1;2 promoters showed a marked increase after 12 h and 18 h of zeocin treatment, respectively (Fig. 7c), which agrees with the observation that MYB3R3-GFP was abundant in the root tip after 12 h (Fig. 2a).
We next compared their binding to the KNOLLE and CYCB1;2 promoters between MYB3R3-GFP, MYB3R3AAA-GFP and MYB3R3DDD-GFP. As shown in Fig. 8a, the binding of MYB3R3-GFP was elevated by zeocin treatment, while that of MYB3R3AAA-GFP was already high in the absence of zeocin. On the other hand, MYB3R3DDD-GFP did not display as dramatic an increase in promoter binding as that observed for MYB3R3-GFP even after 24 h of zeocin treatment. This result correlates with the levels of GFP fusion proteins accumulating in each line (Fig. 6b). Measurement of KNOLLE and CYCB1;2 transcripts showed that they decreased after 24 h of zeocin treatment in myb3r3 expressing MYB3R3-GFP or MYB3R3AAA-GFP to levels comparable to those in WT plants (about 60 and 80% reduction for KNOLLE and CYCB1;2, respectively). However, in myb3r3 expressing MYB3R3DDD-GFP, the transcript levels were less drastically decreased (about 40−50% reduction for both genes) (Fig. 8b). These results suggest that the protein level of MYB3R3 determines its binding to target promoters and the extent of repression of target genes.
When WT seedlings were treated with 2 µM zeocin, transcripts of KNOLLE and CYCB1;2 were reduced and reached a stable level after 18 h (Fig. 9). Those of EDE1 and IMK2 also decreased but reached the stable level after 12 h. In myb3r3 and myb3r5 mutants, transcript accumulation of EDE1 and IMK2 was almost the same as that in WT, but mRNA levels of KNOLLE and CYCB1;2 did not further decrease beyond 12 h of zeocin treatment (Fig. 9). A similar tendency was also observed in myb3r3 for PAKRP1, EHD2 and PLE/MAP65-3, but not for AUR2, PAKRP2 or CDC20.1 (Supplementary Fig. 18). These results suggest that Rep-MYBs function in further suppressing a particular set of G2/M-specific genes at a later stage of the DNA damage response (beyond 12 h of zeocin treatment in our experimental system), thus blocking cell cycle progression.
Previous reports demonstrated that tobacco and Arabidopsis Act-MYBs are phosphorylated and activated by CDK18, 29. Since mitotic cyclins, one of the targets of MYB3Rs, form active CDK-cyclin complexes, Act-MYBs are further phosphorylated and activated through a positive feedback loop, thus causing a burst of G2/M-specific gene expression (Fig. 10, left). In this study, we found that Rep-MYBs are targeted for degradation by proteasomes. Treatment with the CDK-specific inhibitor roscovitine enhanced accumulation of Rep-MYBs, indicating that CDK activity is required for Rep-MYB degradation. Indeed, Rep-MYBs were phosphorylated by CDKs in vitro, and phosphomimic or alanine substitutions at the phosphorylation sites led to lower or higher accumulation of MYB3R3 in roots, respectively. These data suggest that, in the absence of DNA stress, CDK phosphorylates Rep-MYBs and promotes proteasomal degradation (Fig. 10, left). Kobayashi et al.20 reported that Rep-MYBs repress transcription along the cell cycle except for G2/M, and our measurement of nuclear area in the meristem showed that MYB3R3 protein preferentially accumulates around early S phase. Since CDK activity is high at G2/M phase36, it is probable that CDK-mediated control of protein degradation is involved in oscillation of Rep-MYB accumulation during the cell cycle.
In response to DNA damage, the ATM/ATR-SOG1 pathway is activated, and SOG1 induces the expression of CDK suppressors such as SMRs, thereby reducing CDK activity14, 37. We previously reported that MYB3R4 expression is downregulated by DSBs14, and here we revealed that SOG1 is required for a rapid decrease of MYB3R4 transcripts. As a result, Act-MYB-mediated transcription of G2/M-specific genes is suppressed, and lowered CDK activity liberates Rep-MYBs from proteasomal degradation (Fig. 10, right). In support of this idea, we observed zeocin-induced accumulation of Rep-MYBs, which was cancelled by introducing phosphomimic substitutions at the phosphorylation sites. Rep-MYBs then bind to the MSA element and repress the expression of G2/M-specific genes (Fig. 10, right). We showed that this repression occurs at a later stage of the DNA damage response and is essential for inhibiting cell division. We also found that the myb3r3 and myb3r5 mutants exhibited less zeocin-induced stem cell death, which is also a programmed response involving the ATM/ATR-SOG1 pathway15, 16. Therefore, stem cell death is most likely another outcome of Rep-MYB-mediated cell cycle arrest.
Our results indicate that Rep-MYBs function in repressing a particular set of G2/M-specific genes under DNA stress. There is no apparent difference in the core MSA element between target and non-target genes, and differential binding activity of Rep-MYBs may therefore be dependent on promoter contexts of individual G2/M-specific genes. Kobayashi et al.20 demonstrated that Rep-MYBs form a complex with retinoblastoma-related protein and the E2F transcription factor E2FC, indicating that plants have the multiprotein complex known as DREAM/dREAM-like complex in vertebrates and Drosophila. In mammalian cells, the transition from quiescence to mitotic state is accompanied by the release of some subunits of the DREAM complex, in conjunction with the recruitment of the Myb transcription factor B-MYB, which activates transcription of various genes that are essential for mitosis38. It is therefore probable that Arabidopsis Rep-MYBs belong to a distinct DREAM complex under DNA stress conditions, and that another subunit participates in controlling target specificity together with Rep-MYBs. Biochemical and genetic studies on the DREAM complex may elucidate the mechanism regulating a distinct set of G2/M-specific genes under DNA damage conditions.
During protein degradation by the 26S proteasome, phosphorylation of target proteins provides a recognition signal for an E3 ubiquitin ligase, especially for SCF (SKP1-CUL1-F-box) complexes39. In the case of Sic1, one of the CDK inhibitors in budding yeast, phosphorylation of a minimum of six residues by Cdc28 sets a threshold for SCF recognition, thereby providing a sensitive switch mechanism for the induction of the G1/S transition40. Here, we show that CDKs can phosphorylate Rep-MYBs in vitro, and that CDK activities are required for their degradation by proteasomes. On the other hand, CDK is known to phosphorylate and activate Act-MYBs18, 29. This implies that CDK acts on Act- and Rep-MYBs in opposite directions, and that once CDK activities decrease in response to DNA damage, the mitotic state can switch to the quiescent state in a rapid and precise manner. It is therefore possible that other stresses that cause a reduction in CDK activity also induce MYB3R-mediated inhibition of cell division. Further studies will identify the E3 ubiquitin ligase that recognizes Rep-MYBs and reveal how it is involved in stress responses by controlling the cell cycle.
Plant materials and growth conditions
WT and mutant plants used in this study are all in the Col-0 background. lig4, sog1-1, the T-DNA insertion mutants myb3r3-1 (SALK_041111), myb3r3-2 (GABI 546A07), myb3r4-2 (SALK_034806) and myb3r5-1 (SALK_031972), and the transgenic line carrying ProMYB3R3::MYB3R3-GFP were described previously11, 18, 20, 24. myb3r4-3 (GABI 634B01) and myb3r5-2 (GABI 252G10) were also used in this study. To generate ProMYB3R5::MYB3R5-GFP, the promoter and the coding region of MYB3R5 were PCR-amplified from genomic DNA using primers listed in Supplementary Table 1, and cloned into the Gateway entry vector pDONR221 (Invitrogen) by a BP reaction. An LR reaction was performed with the destination vector pGWB55041 to generate a binary vector carrying the fusion construct with GFP. Alanine or aspartic acid substitutions were introduced by PCR using primers listed in Supplementary Table 1. Plants were grown in MS medium under continuous light conditions at 22 °C. Chemicals used in this study are zeocin (Invitrogen), bleomycin (Wako), bleocin (Wako), hydroxyurea (Nacalai Tesque), aphidicolin (Sigma), boric acid (Sigma), MG132 (Biomol) and roscovitine (Wako). Gamma-ray irradiation was conducted with a 137Cs source (Radiation Biology Center, Kyoto University). For counting the number of true leaves, seeds were grown in MS medium for 10 days.
Seedlings were immersed in 0.1 mg ml−1 propidium iodide solution for 3 min, and observed with a confocal laser scanning microscope (FV1000, Olympus). Root meristem size was measured by counting the number of cortex cells between the quiescent centre and the first elongated cell. GFP fluorescence was quantified by AxioVision Rel. 4.8 software (Carl Zeiss).
Nuclear area measurement
Roots were fixed in MTSB (5 mM PIPES, 0.5 mM EGTA, 0.35 mM MgSO4 7H2O, 8.9 mM KOH, pH 8.0) containing 3% (w/v) paraformaldehyde and 0.5% (v/v) Triton X-100 for 20 min at room temperature. After washing with phosphate-buffered saline (PBS), cell walls were partially digested in an enzyme solution containing 0.05% (w/v) pectolyase (Kyowa Kasei) and 0.15% (v/v) Triton X-100 for 2 h at 30 °C. The roots were then washed with PBS and stained with 1 µg ml−1 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at room temperature. Fluorescence images of epidermal trichoblast cells in the root meristem were taken from a Z-stack of 20 confocal images at 0.4 µm intervals, and the resulting images were processed using ImageJ software to measure the nuclear area.
Double staining with EdU and DAPI
EdU staining was conducted with a Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s instruction. In brief, seedlings were immersed in liquid MS medium containing 10 μM EdU for 60 min, and fixed in MTSB containing 3% (w/v) paraformaldehyde and 0.5% Triton X-100 for 20 min. After washing twice with PBS, samples were incubated in the Click-iT reaction cocktail for 30 min in the dark. The reaction cocktail was then removed, and samples were washed with PBS, followed by cell wall digestion and DAPI staining as described for nuclear area measurement.
Pulse labelling with EdU
Four-day-old WT seedlings were grown on solid medium containing different concentrations of zeocin for 12 h and transferred to liquid MS medium containing zeocin and 20 µM EdU, followed by a 15-min incubation. After washing with MS medium, seedlings were again grown on zeocin-containing medium for different chase time periods, and subjected to double staining with EdU and DAPI as described above.
Total protein extracted from root tips was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene difluoride membrane (Millipore). Immunoblotting was conducted with anti-GFP antibody (11814460001, Roche), anti-α-tubulin antibody (ab4074, Abcam) or anti-histone H3 antibody (AS10 710, Agrisera) at a dilution of 1:2000, 1:3000 or 1:5000, respectively. Clarity Western ECL Substrate (Bio-Rad Laboratories) was used for detection. The intensity of each band was measured by Image Lab software (Bio-Rad Laboratories), and relative values were calculated by normalizing with respect to the bands of controls. Uncut blot images are shown in Supplementary Figs. 19, 20 and 22.
In vitro kinase assay
Whole or partial MYB3R open reading frames were cloned into the pENTR/D-TOPO vector (Invitrogen), and then transferred to the pDEST17 vector (Invitrogen) using LR Clonase (Invitrogen) to be in-frame with the 6× His tag. Alanine substitutions were introduced by PCR using primers listed in Supplementary Table 1. His-tagged MYB3R proteins were purified with Ni Sepharose 6 Fast Flow (GE Healthcare) according to the manufacturer’s instruction. For in vitro kinase assay, CDKs were immunoprecipitated from MM2d cultured cells with specific antibodies, and the immunoprecipitates were incubated with 1 μg His-tagged substrates in 10 µl assay buffer (50 mM Tris-HCl, 15 mM MgCl2, 5 mM EGTA, 1 mM dithiothreitol, 0.01 mM ATP, 0.185 MBq of [γ-32P] ATP, pH 7.5) at 30 °C for 15 min. The reaction was stopped by the addition of sample buffer for SDS-PAGE, boiled for 5 min, and loaded onto a polyacrylamide gel. Phosphorylated proteins were detected with an imaging analyzer (BAS-1800, Fujifilm). Uncut autoradiography images are shown in Supplementary Fig. 21.
In vitro degradation assay
One hundred micrograms of root extracts from Arabidopsis seedlings was incubated with 50 ng of His-MYB3R3 or His-MYB3R3AAA in a buffer containing 25 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 5 mM dithiothreitol, 10 mM NaCl and 10 mM ATP. After protein separation by SDS-PAGE, immunoblotting was conducted using anti-His antibodies (11922416001, Roche) at a 1:5000 dilution and an ECL Western Blotting Detection Kit (GE Healthcare).
Two grams of 10-day-old ProMYB3R3::MYB3R3-GFP seedlings were collected and fixed in 100 ml of 1% (w/w) formaldehyde under vacuum for 30 min. After washing twice with ice-cold 0.2 M glycine, the plant materials were ground into powder with liquid nitrogen and lysed in 40 ml nuclear isolation buffer [1 M hexylene glycol, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 0.5% Triton X-100, 5 mM 2-mercaptoethanol, 1× protease inhibitor cocktail (Roche)]. Nuclei were separated by filtration through Miracloth (Calbiochem), and isolated nuclei were suspended in 6 ml lysis buffer (50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1% Triton X-100, 1× protease inhibitor cocktail (Roche)) and sonicated. Chromatin complexes bound to MYB3R3-GFP were immunoprecipitated with anti-GFP antibody (GF200, Nacali Tesque) at a 1:1000 dilution. To quantify the precipitated chromatin, gene-specific primers (Supplementary Table 1) were used for real-time qPCR.
Total RNA was extracted from Arabidopsis roots with an RNeasy Plant Mini Kit (QIAGEN). First-strand cDNAs were prepared from total RNA using the Superscript II First-Strand Synthesis System (Invitrogen) according to the manufacturer’s instruction. Quantitative PCR was performed with a THUNDERBIRD SYBR qPCR Mix (Toyobo) with 100 nM primers and 0.1 µg of first-strand cDNAs. Primer sequences are listed in Supplementary Table 1. PCR reactions were conducted with the LightCycler 480 Real-Time PCR System (Roche) under the following conditions: 95 °C for 5 min; 45 cycles of 95 °C for 10 s, 60 °C for 10 s and 72 °C for 15 s. ACTIN2 (At3g18780) was used as a control.
Identification of phosphorylation sites
For production of CDKA;1-CYCD3;1 complexes, E. coli. BL21 strain was transformed with the two plasmids, pCDFDuet-CDKA;1 and pHGGWA-CYCD3;1, which carry StrepIII-CDKA;1 and GST-CYCD3;1, respectively35. Cells were grown in 50 ml of LB medium containing 50 μg ml−1 ampicillin and 50 μg ml−1 spectinomycin at 37 °C to OD600 of 0.6. The production of proteins was induced by adding IPTG to 0.3 mM, and cells were cultured overnight at 18 °C. After centrifugation, the cell pellet was resuspended in 2.5 ml binding buffer (50 mM NaH2PO4, 100 mM NaCl, 10% (v/v) glycerol, 25 mM imidazole, pH 8.0) and lysed by sonication. The cell slurry was then centrifuged at 10,000×g for 40 min at 4 °C, and the supernatant was applied onto an Econopack column (Bio-rad) packed with 300 μl Ni-NTA resin (Qiagen). After washing sequentially with 3 ml binding buffer, CDKA;1−CYCD3;1 complexes were eluted with 600 μl elution buffer (50 mM NaH2PO4, 100 mM NaCl, 10% (v/v) glycerol, 225 mM imidazole, pH 8.0). For phosphorylation reaction, the buffer was exchanged to the kinase buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EGTA, pH 7.5) containing 1× protease inhibitors cocktail (Roche). MYB3R3 and MYB3R5 open reading frames were transferred from the pENTR/D-TOPO vector to the pDEST15 vector (Invitrogen) using LR Clonase to be in-frame with the GST tag. GST-MYB3R3 and GST-MYB3R5 were purified with Glutathione Sepharose 4B (GE Healthcare) according to the manufacturer’s instruction.
A phosphorylation reaction was performed using 1 mg of GST-MYB3R3 and GST-MYB3R5 as substrate. After separation of reaction mixtures by SDS-PAGE, proteins were stained with Coomassie Brilliant Blue, and the bands corresponding to GST-MYB3R3 and GST-MYB3R5 were subjected to in-gel digestion using Sequencing Grade Modified Trypsin (Promega). LC−MS/MS analysis was performed using an LTQ-orbitrap XL-HTC-PAL system (Thermo Fisher Scientific)42. Obtained spectra were compared with a protein database (TAIR10) using the Mascot server (version 2.4). The Mascot search parameters were as follows: ion-score cut-off with the threshold at 0.05; peptide tolerance at 10 p.p.m.; MS/MS tolerance at ± 0.8 Da; peptide charge of 2+ or 3+; trypsin as enzyme allowing up to two missed cleavages; carbamidomethylation on cysteine as a fixed modification and oxidation on methionine and phosphorylation on serine, threonine and tyrosine as variable modifications.
All data supporting the findings of this study are available within the article and its Supplementary Information files or from the corresponding author on request.
Rounds, M. A. & Larsen, P. B. Aluminum-dependent root-growth inhibition in Arabidopsis results from AtATR-regulated cell-cycle arrest. Curr. Biol. 18, 1495–1500 (2008).
Roldán-Arjona, T. & Ariza, R. R. Repair and tolerance of oxidative DNA damage in plants. Mutat. Res. 681, 169–179 (2009).
Sakamoto, T. et al. Condensin II alleviates DNA damage and is essential for tolerance of boron overload stress in Arabidopsis. Plant Cell 23, 3533–3546 (2011).
Song, J. & Bent, A. F. Microbial pathogens trigger host DNA double-strand breaks whose abundance is reduced by plant defense responses. PLoS Pathog. 10, e1004030 (2014).
Bensimon, A., Aebersold, R. & Shiloh, Y. Beyond ATM: the protein kinase landscape of the DNA damage response. FEBS Lett. 585, 1625–1639 (2011).
Flynn, R. L. & Zou, L. ATR: a master conductor of cellular responses to DNA replication stress. Trends Biochem. Sci. 36, 133–140 (2011).
Cheng, Q. & Chen, J. Mechanism of p53 stabilization by ATM after DNA damage. Cell Cycle 9, 472–478 (2010).
Smith, J., Tho, L. M., Xu, N. & Gillespie, D. A. The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. Adv. Cancer Res. 108, 73–112 (2010).
Garcia, V. et al. AtATM is essential for meiosis and the somatic response to DNA damage in plants. Plant Cell 15, 119–132 (2003).
Culligan, K., Tissier, A. & Britt, A. ATR regulates a G2-phase cell-cycle checkpoint in Arabidopsis thaliana. Plant Cell 16, 1091–1104 (2004).
Yoshiyama, K., Conklin, P. A., Huefner, N. D. & Britt, A. B. Suppressor of gamma response 1 (SOG1) encodes a putative transcription factor governing multiple responses to DNA damage. Proc. Natl Acad. Sci. USA 106, 12843–12848 (2009).
Yoshiyama, K. O. et al. ATM-mediated phosphorylation of SOG1 is essential for the DNA damage response in Arabidopsis. EMBO Rep. 14, 817–822 (2013).
Sjogren, C. A., Bolaris, S. C. & Larsen, P. B. Aluminum-dependent terminal differentiation of the Arabidopsis root tip is mediated through an ATR-, ALT2-, and SOG1-regulated transcriptional response. Plant Cell 27, 2501–2515 (2015).
Adachi, S. et al. Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis. Proc. Natl Acad. Sci. USA 108, 10004–10009 (2011).
Fulcher, N. & Sablowski, R. Hypersensitivity to DNA damage in plant stem cell niches. Proc. Natl Acad. Sci. USA 106, 20984–20988 (2009).
Furukawa, T. et al. A shared DNA-damage-response pathway for induction of stem-cell death by UVB and by gamma irradiation. DNA Repair 9, 940–948 (2010).
Ito, M. Conservation and diversification of three-repeat Myb transcription factors in plants. J. Plant Res. 118, 61–69 (2005).
Haga, N. et al. R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana. Development 134, 1101–1110 (2007).
Haga, N. et al. Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis. Plant Physiol. 157, 706–717 (2011).
Kobayashi, K. et al. Transcriptional repression by MYB3R proteins regulates plant organ growth. EMBO J. 34, 1992–2007 (2015).
Berdy, J. in Amino Acid and Peptide Antibiotics (ed. Berdy, J.) 459–497 (CRC Press, 1980).
Saban, N. & Bujak, M. Hydroxyurea and hydroxamic acid derivatives as antitumor drugs. Cancer Chemother. Pharmacol. 64, 213–221 (2009).
Sala, F. et al. Specific and reversible inhibition by aphidicolin of the α-like DNA polymerase of plant cells. FEBS Lett. 117, 93–98 (1980).
Hefner, E., Huefner, N. & Britt, A. B. Tissue-specific regulation of cell-cycle responses to DNA damage in Arabidopsis seedlings. DNA Repair 5, 102–110 (2006).
Culligan, K. M., Robertson, C. E., Foreman, J., Doerner, P. & Britt, A. B. ATR and ATM play both distinct and additive roles in response to ionizing radiation. Plant J. 48, 947–961 (2006).
Jovtchev, G., Schubert, V., Meister, A., Barow, M. & Schubert, I. Nuclear DNA content and nuclear cell volume are positively correlated in angiosperms. Cytogenet. Genome Res. 114, 77–82 (2006).
Hayashi, K., Hasegawa, J. & Matsunaga, S. The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion. Sci. Rep. 3, 2723 (2013).
Yin, K. et al. A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis. Plant. J. 80, 541–552 (2014).
Araki, S., Ito, M., Soyano, T., Nishihama, R. & Machida, Y. Mitotic cyclins stimulate the activity of c-Myb-like factors for transactivation of G2/M phase-specific genes in tobacco. J. Biol. Chem. 279, 32979–32988 (2004).
Wang, F. et al. Biochemical insights on degradation of Arabidopsis DELLA proteins gained from a cell-free assay system. Plant Cell 21, 2378–2390 (2009).
Inagaki, S. & Umeda, M. Cell-cycle control and plant development. Int. Rev. Cell Mol. Biol. 291, 227–261 (2011).
Kono, A. et al. Arabidopsis D-type cyclin CYCD4;1 is a novel cyclin partner of B2-type cyclin-dependent kinase. Plant Physiol. 132, 1315–1321 (2003).
Adachi, S., Uchimiya, H. & Umeda, M. Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana. Plant Cell Physiol. 47, 1683–1686 (2006).
Takatsuka, H., Ohno, R. & Umeda, M. The Arabidopsis cyclin-dependent kinase-activating kinase CDKF;1 is a major regulator of cell proliferation and cell expansion but is dispensable for CDKA activation. Plant J. 59, 475–487 (2009).
Harashima, H. & Schnittger, A. Robust reconstitution of active cell-cycle control complexes from co-expressed proteins in bacteria. Plant Methods 8, 23 (2012).
De Veylder, L., Larkin, J. C. & Schnittger, A. Molecular control and function of endoreplication in development and physiology. Trends Plant Sci. 16, 624–634 (2011).
Yi, D. et al. The Arabidopsis thaliana SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 control the DNA damage checkpoint in response to reactive oxygen species. Plant Cell 26, 296–309 (2014).
Sadasivam, S., Duan, S. & DeCaprio, J. A. The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression. Genes Dev. 26, 474–489 (2012).
Glickman, M. H. & Ciechanover, A. The ubiquitin-proteasome proteolytic pathway: Destruction for the sake of construction. Physiol. Rev. 82, 373–428 (2002).
Nash, P. et al. Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication. Nature 414, 514–521 (2001).
Nakagawa, T. et al. Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Biosci. Biotechnol. Biochem. 71, 2095–2100 (2007).
Fujiwara, M. et al. Interactomics of Qa-SNARE in Arabidopsis thaliana. Plant Cell Physiol. 55, 781–789 (2014).
We would like to thank Anne B. Britt for sog1-1 seeds. This work was supported by MEXT KAKENHI (Grant numbers 22119009 and 24657033), JSPS KAKENHI (Grant numbers 26291061 and 26650099) and JST, CREST (Grant number JPMJCR12B2).
The authors declare no competing financial interests.
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Chen, P., Takatsuka, H., Takahashi, N. et al. Arabidopsis R1R2R3-Myb proteins are essential for inhibiting cell division in response to DNA damage. Nat Commun 8, 635 (2017). https://doi.org/10.1038/s41467-017-00676-4
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