Lysyl oxidase-like 3 (LOXL3) is a member of the lysyl oxidase family comprising multifunctional enzymes with depicted roles in extracellular matrix maturation, tumorigenesis, and metastasis. In silico expression analyses followed by experimental validation in a comprehensive cohort of human cell lines revealed a significant upregulation of LOXL3 in human melanoma. We show that LOXL3 silencing impairs cell proliferation and triggers apoptosis in various melanoma cell lines. Further supporting a pro-oncogenic role in melanoma, LOXL3 favors tumor growth in vivo and cooperates with oncogenic BRAF in melanocyte transformation. Upon LOXL3 depletion, melanoma cells display a faulty DNA damage response (DDR), characterized by ATM checkpoint activation and inefficient ATR activation leading to the accumulation of double-strand breaks (DSBs) and aberrant mitosis. Consistent with these findings, LOXL3 binds to proteins involved in the maintenance of genome integrity, in particular BRCA2 and MSH2, whose levels dramatically decrease upon LOXL3 depletion. Moreover, LOXL3 is required for efficient DSB repair in melanoma cells. Our results reveal an unexpected role for LOXL3 in the control of genome stability and melanoma progression, exposing its potential as a novel therapeutic target in malignant melanoma, a very aggressive condition yet in need for more effective treatment options.
Lysyl oxidase-like 3 (LOXL3) is a member of the lysyl oxidase (LOX) protein family that comprises five closely related members, prototypical LOX and four LOX-like enzymes (LOXL1–4) . LOX proteins are primarily known for their role as extracellular enzymes; upon secretion they promote stabilization of collagen and elastin fibers contributing to extracellular matrix (ECM) maturation [2,3,4]. Beyond ECM cross-linking, lysyl oxidases have been involved in gene transcription, epithelial to mesenchymal transition (EMT), development, differentiation, and angiogenesis, as well as in distinct pathologies such as fibrosis and cancer (reviewed in refs. [5,6,7,8]). Some of the emerging roles of several LOX members are independent of their secretion and have been associated with their intracellular and intranuclear localization [5, 6, 8, 9]. Moreover, the amine oxidase catalytic activity is not always required for some of the recently reported lysyl oxidase functions, including their involvement in angiogenesis, EMT, and inflammation [6, 9,10,11], suggesting complex and wide-ranging roles for the members of the LOX family.
Human LOXL3 presents differential tissue expression regarding other LOX proteins [12,13,14,15] and has been recently proposed as a candidate gene responsible for recessive autosomal Stickler syndrome , a collagenopathy , whereas null mutations in LOXL3 have been associated with early-onset high myopia . Genetic inactivation in mice has revealed Loxl3 involvement in skeletal, muscular, and lung development [19,20,21]. Thus far the reported roles for LOXL3 were essentially associated with LOXL3 extracellular activity regarding ECM maturation, whereas LOXL3 involvement in cancer remains limited. Our previous studies identified human LOXL3 as modulator of EMT and Snail1 functional activity  and LOXL3 has been involved in the nuclear regulation of Stat3 activity . Since the involvement of several LOX members in cancer has been extensively characterized [5, 6, 8], we explored LOXL3 contribution to human cancer by interrogating a comprehensive set of human cancer samples for LOXL3 expression that unveiled an association of LOXL3 to melanoma. We, therefore, performed gain and loss-of-function experiments to determine the contribution of LOXL3 to melanoma pathogenesis. Our studies reveal that human melanoma cells are addicted to LOXL3 expression since LOXL3 knockdown halts cell proliferation and triggers apoptosis. Moreover, LOXL3 cooperates to malignant transformation and contributes to melanomagenesis. We have found that LOXL3 binds to proteins that protect genome integrity (BRCA2, MSH2, SMC1A, NUMA1) and that its absence promotes a defective DNA damage checkpoint activation, deficient DNA repair and aberrant mitosis in melanoma cells. Our data uncover an unprecedented role for LOXL3 in melanoma biology and support the relevance of LOXL3 as a novel druggable target for therapeutic intervention in this severe disease.
LOXL3 is overexpressed in human melanoma
In order to explore the involvement of LOXL3 in cancer, we performed in silico analyses of public data sets. Mining of the Cancer Cell Line Encyclopedia (CCLE) database (www.broadinstitute.org/ccle) rendered LOXL3 gene expression levels in 1036 cancer cell lines . Besides Hodgkin lymphoma and glioma, LOXL3 highest expression was found in melanoma and chondrosarcoma (Fig. 1a). Considering LOXL3 mRNA expression levels and the cell lines corresponding to each tumor type analyzed (n = 61 for melanoma, n = 4 for chondrosarcoma)  we further evaluated LOXL3 role in melanoma. Data mining of The Cancer Genome Atlas (TCGA), including whole genome sequencing and transcriptomic profiles of more than 300 cutaneous melanoma patients , revealed upregulated LOXL3 mRNA levels significantly associated with the presence of well-known driver mutations in melanoma. In particular, LOXL3 levels were higher among tumors harboring BRAF activating mutations whereas a similar trend, without reaching statistical significance, was observed for the NRAS and the NF1 subtype compared to the LOXL3 mRNA levels found in the triple WT subtype (Fig. 1b). The latter comprised all those samples not harboring mutations in any of the aforementioned genes, suggesting a link between canonical MAPK pathway activation and LOXL3 upregulation in melanoma. Moreover, examination of published melanoma transcriptomic profiles [25, 26] and TCGA data  for LOXL3 expression confirmed a significant upregulation of LOXL3 levels in both primary and metastatic melanoma patient-derived samples compared to controls (Fig. 1c, d), while no differences in expression were found between primary and metastatic samples (Fig. 1c, d; Supplementary Fig. 1a). Accordingly, analysis of a large cohort of primary and metastatic melanoma cell lines (Supplementary Table 1) confirmed increased LOXL3 expression at both LOXL3 mRNA and protein levels in melanoma cells compared to primary and immortalized human melanocytes (Fig. 1e, f). As depicted in Fig. 1f, canonical LOXL3 and a splicing variant lacking exons 4 and 5 (LOXL3ΔE4E5 or LOXL3Δ) (Supplementary Fig 1b, c), were detected by western blot. Both LOXL3 isoforms were found similarly expressed in most melanoma cells tested (Fig. 1f). LOXL3Δ isoform probably corresponds to the recently identified splice variant LOXL3-sv2 . LOXL3 expression is thus associated with both primary and metastatic melanomas, and considerably upregulated compared to other LOX family members frequently associated with cancer, such as LOX and LOXL2 [5, 6] (Fig. 1f). These results suggest a previously unexplored role for LOXL3 in melanoma.
Silencing LOXL3 expression in melanoma induces cell death
To investigate LOXL3 involvement in melanoma, we initially evaluated the effects of LOXL3 silencing in a panel of human melanoma cells. We designed two different short hairpin RNAs (shRNAs) against LOXL3 also targeting LOXL3Δ without altering LOX and LOXL2 protein levels (Supplementary Fig. 2a). All melanoma cell lines depleted for LOXL3 expression halt proliferation between 5–8 days post-infection (dpi) compared to control cells infected with a non-targeting control (NTC) (Fig. 2a). LOXL3 depletion had analogous effects on cells harboring BRAF (A375P, WM98-1, and SK-MEL-28) or NRAS (SK-MEL-147) activating mutations as well as on cells wild type for NRAS/BRAF lacking NF1 protein (MeWo)  (Fig. 2a and Supplementary Table 1). Moreover, LOXL3 silencing was deleterious for primary (A375P and WM98-1) and metastatic melanoma cell lines (SK-MEL-28, SK-MEL-147, and MeWo) corroborating that melanoma cells require LOXL3 expression to survive independently of their mutational status or the disease stage from which they were derived. We then analyzed whether the absence of LOXL3 prevents cell growth by triggering apoptosis. Indeed, LOXL3-depleted cell lines displayed caspase-3 activation (Fig. 2b) and increased annexin V staining (Fig. 2c) at 5–6 dpi compared to control NTC-transduced cells. Likewise, efficient LOXL3 silencing by transient siRNA transfection induced a similar level of apoptosis as shRNA transduction in SK-MEL-28 cells as shown by caspase-3 cleavage and annexin V staining (Supplementary Fig. 2b). Moreover, the CRISPR/Cas9-mediated knockout of LOXL3 in A375P cells also promoted a cell proliferation delay accompanied by an increase in apoptotic cell death (Supplementary Fig. 2c–e). Taken together, we conclude that human melanoma cell lines expressing LOXL3 are addicted to the maintenance of its expression.
LOXL3 promotes tumorigenesis
To assess LOXL3 contribution to melanomagenesis, we overexpressed LOXL3 in immortalized melanocytes expressing BRAFV600E (HMEL)  with insignificant levels of endogenous LOXL3 (Fig. 1f and Fig. 3a). This cell line allows investigating the cooperation of genetic alterations to neoplastic transformation. Stable LOXL3 and LOXL3Δ expression in HMEL cells greatly enhanced their anchorage independent growth, a hallmark of transformation (Fig. 3b) without affecting cell proliferation (Fig. 3c). Additionally, LOXL3 and LOXL3Δ ectopic expression favored migration and invasiveness of HMEL cells (Fig. 3d, e). Similarly, LOXL3 transduction in a metastatic melanoma cell line (501MEL) harboring minimal endogenous LOXL3 levels (Fig. 1f and Supplementary Fig. 3a) promoted migration and invasion without affecting cell proliferation (Supplementary Fig. 3b–d), whereas endogenous LOXL3 silencing had analogous effects as previously shown for other melanoma cell lines (Supplementary Fig. 3e–g). Besides, tumorigenic A375P cells depleted for LOXL3 (Fig. 2b) showed increased latency and delayed tumor growth compared to control cells (Fig. 3f). Thus, enhanced LOXL3 expression cooperates with oncogenic alterations such as active BRAF to transform human melanocytes favoring migration and invasion of melanoma cells and supporting melanoma tumorigenicity.
LOXL3 binding partners are involved in mitosis and the DNA damage response (DDR)
We carried out a comprehensive proteomic analysis of LOXL3 protein interactors to further understand LOXL3 involvement in melanomagenesis. Since we could not discriminate specific roles for LOXL3 and LOXL3Δ, we ectopically expressed both FLAG-tagged isoforms in metastatic melanoma 501MEL cell line (Supplementary Fig. 3a) and performed an anti-FLAG immunopurification followed by mass spectrometry analysis. After analysis and filtering, we focused on those common proteins identified in eluates from LOXL3 and LOXL3Δ transduced cells, falling a number of them (with 5 to 64 identified peptides) within the functional categories of mitosis and/or DDR (Fig. 4a and Supplementary Table 2). To validate the interactions, we performed immunoprecipitation experiments in 501MEL and A375P melanoma cells expressing ectopic LOXL3 or LOXL3Δ FLAG-tagged proteins. We detected specific binding of LOXL3 to BRCA2, SMC1A, MSH2, and NUMA1 (Fig. 4b), proteins involved in DDR and/or mitosis [30,31,32,33], suggesting a role for LOXL3 in these biological processes. Binding of endogenous LOXL3 to endogenous BRCA2, NUMA1, and SMC1A proteins was also confirmed (Fig. 4c and Supplementary Fig. 4a). LOXL3 localization has been described as cytoplasmic/perinuclear  and nuclear . Consistent with LOXL3 binding to recognized nuclear partners, cellular fractionation experiments in A375P cells revealed LOXL3 presence in the nuclear compartment (Supplementary Fig. 4b). Further differential centrifugation experiments located LOXL3 and its binding partners in the same enriched cytoplasmic and nuclear subcellular fractions (Supplementary Fig. 4c) supporting their biological association. Moreover, confocal microscopy analyses determined LOXL3 and BRCA2 co-localization in the cytoplasm and perinuclear area of melanoma cells (Fig. 4d).
Melanoma cells activate the DDR upon LOXL3 knockdown
The interaction of LOXL3 with proteins involved in repairing damaged DNA at double-strand breaks (DSBs) (Fig. 4) led us to hypothesize the association of LOXL3 to DDR checkpoint activation and/or DSB repair. LOXL3-silenced A375P cells accumulated phosphorylated H2AX (γH2AX) foci (Fig. 5a, d, g), a marker of DSBs , as well as 53BP1 foci (Fig. 5b, e), a mediator of DSB repair (reviewed in ref. ) to similar levels to those induced by known DNA damage agents, such as hydroxyurea or ionizing radiation (Supplementary Fig. 5a, b). Noticeably, at early time points post-lentiviral infection (32–56 h pi), and before any effects on cell proliferation nor apoptosis were detected (Fig. 2 and Supplementary Fig. 6a), LOXL3-silenced cells showed activation of ATM (Fig. 5c, f and Supplementary Fig. 5c), a master checkpoint kinase activated after DSBs (Supplementary Fig. 5d) and required for DNA repair and genome stability . Concomitantly, LOXL3 silencing promoted phosphorylation of the ATM effector checkpoint substrate, Chk2 (Fig. 5g). In contrast, activation of ATR, another central kinase mediating DDR , is reduced shortly after LOXL3 knockdown, as levels of pATR and its immediate target, Chk1, remarkably fell upon LOXL3 silencing (Fig. 5g). In fact, the levels of other relevant downstream effectors of ATM and ATR pathways that ultimately mediate the repair of DNA damage such as BRCA1, BRCA2, Rad51, and MSH2 [37, 38], dropped shortly after LOXL3 depletion (Fig. 5g and Supplementary Fig. 5e), whereas NUMA1 and SMC1A levels remained stable (Fig. 5h). p53 and p21 levels increased initially consistent with DDR activation  but p53 levels were not steadily maintained afterwards (Fig. 5h). Indeed, cells depleted for LOXL3 displayed reduced ability to repair DSBs as shown using the DRGFP reporter to measure the frequency of homologous recombination (HR)  (Fig. 5i) while non-homologous end joining (NHEJ)  repair pathway was not altered (Supplementary Fig. 5f). Altogether, these results suggest that LOXL3 expression in melanoma cells allows for DNA damage repair by maintaining the protein levels of BRCA2, Rad51, and MSH2. Besides, upon LOXL3 silencing, melanoma cells accumulate DSBs and subsequently activate the ATM checkpoint kinase but fail to induce a complete DDR.
LOXL3 is required for proper mitotic completion
We then analyzed the cell-cycle progression of melanoma cells and unveiled a consistent accumulation of cells in G2/M phase in the absence of LOXL3 compared to control cells (Supplementary Fig. 6a). At the molecular level, upon LOXL3 silencing, MYC levels decreased along with p21 and p27 upregulation (Fig. 5h), and the protein levels of mitotic regulators such as AURKA, cyclin A and cyclin B1 were diminished while CDK1 and cyclin D levels did not significantly change compared to control cells (Fig. 5h). Since LOXL3-depleted melanoma cells ultimately die after shRNA transduction (Fig. 2), we analyzed how A375P cells were able to enter and exit mitosis in the absence of LOXL3 at early time points after infection. G1/S synchronized A375P control and LOXL3-depleted cells were released into fresh media to follow their cell-cycle progression by FACS. The total percentage of A375P cells that entered G2/M phase was not affected by LOXL3 silencing, whereas a significant delay in the ability of LOXL3-depleted cells to exit G2/M compared to control NTC cells was detected (Supplementary Fig. 6b, c), suggesting an essential role for LOXL3 in G2/M completion. Correspondingly, we observed that endogenous LOXL3 protein levels accumulated in cells during G2/M phase (Supplementary Fig. 6d). To further investigate cell-cycle progression, LOXL3 shRNAs and scrambled (Ctrl) shRNA were subcloned into lentiviral vectors expressing histone 2B-green fluorescent protein (H2B-GFP) or histone 2B-red fluorescent protein (H2B-RFP), respectively . Control (red) and LOXL3 knockdown A375P cells (green) were synchronized in G1/S, released into fresh media and their mitotic progression was analyzed by fluorescence time-lapse imaging from 4 to 48 h. We observed that while 84% of control cells entered mitosis (122 out of 146, Fig. 6a) taking approximately 2 h for completion (Fig. 6b), only 49% of shL3#1 knockdown cells entered mitosis (79 out of 162, Fig. 6a) and progressed slower (Fig. 6b). Note that even fewer A375P cells transduced with shL3#2 were able to enter mitosis (16%, 39 out of 241, Fig. 6a). Particularly, LOXL3-silenced cells spent more time to progress to anaphase than control cells (Fig. 6b) and a remarkable fraction of LOXL3-silenced cells underwent apoptosis before entering mitosis compared to control cells (Fig. 6c). Moreover, close to 10% of LOXL3 knockdown cells attempting to divide died through mitotic catastrophe (Fig. 6f, lower panels, and Supplementary Fig. 6e). LOXL3-depleted A375P cells also displayed mitotic abnormalities such as an increased number of micronuclei (Fig. 6d, f) often related to chromosome segregation defects . Besides, most of these micronuclei also accumulated γH2AX (Fig. 5a and Fig. 6e), a marker of genomic instability. Finally, LOXL3 silencing entailed faulty chromosome alignment in the metaphase plate and the presence of lagging chromosomes upon anaphase was also detected (Fig. 6f, lower panels). The alterations in cell-cycle progression can be observed in representative videos from A375P LOXL3-silenced cells compared to control cells (Supplementary Videos V1-V3). Altogether, these data indicate that the presence of LOXL3 is required for proper mitotic progression.
Upregulation of LOXL3 in melanoma contributes to genomic stability
Due to the aforementioned results involving LOXL3 in DNA repair mechanisms and mitosis linked to proper chromosome distribution, we hypothesized that LOXL3 might be required to maintain genome stability of melanoma cells. Data mining from the TCGA  showed that upregulated LOXL3 mRNA levels significantly correlate with a higher presence of copy number alterations (CNA) and overall mutations in human melanoma samples (n > 300 patients) (Fig. 7a, b). We then performed conventional comparative genomic hybridization (CGH) profiling of several primary and metastatic cell lines upon LOXL3 silencing, identifying many genomic aberrations associated with the loss of LOXL3 expression (Supplementary Table 3) additionally supporting LOXL3 contribution to genomic integrity in melanoma.
Finally, we explored potential mechanisms responsible for LOXL3 upregulation in melanoma tumors, in particular epigenetic control. TCGA data interrogation  revealed that LOXL3 promoter methylation levels (detected with four independent probes) negatively correlate with LOXL3 mRNA expression in melanoma tumors (Fig. 7c). Remarkably, the percentage of methylation of LOXL3 regulatory regions inversely associates with LOXL3 expression levels in diverse melanoma cells (Fig. 7d, Fig. 1e and Supplementary Fig. 7). Moreover, upon inhibition of DNA methylation, LOXL3 is upregulated in melanoma cells with minimal endogenous LOXL3 levels (Fig. 7e). These data suggest that the hypomethylation of LOXL3 promoter in melanoma is one factor leading to enhanced LOXL3 expression.
Recently, accumulating evidence has contributed to recognize the importance of lysyl oxidase family members in a plethora of biological and pathological processes ranging from ECM maturation to fibrosis, tumorigenesis and metastasis [5, 6, 8, 43]. LOX proteins have a well-established role as secreted enzymes contributing to tissue homeostasis by cross-linking ECM substrates [2, 3]. Nonetheless, the generation of independent loss-of-function mouse models of Lox, Loxl1, Loxl2, and Loxl3 genes has revealed non-redundant roles and distinct contribution to tissue fitness and proper embryonic development [19,20,21, 44,45,46]. In addition to their extracellular roles, recent data support equally important intracellular functions for some LOX family members [6,7,8,9, 11]. Extensive work by our lab and others has established the deregulation of lysyl oxidases in cancer and their status has been associated with patient outcome in specific neoplasias [5, 6]. Regarding LOXL3, substantial information reinforces its role in ECM remodeling linking deregulated LOXL3 to different connective tissue disorders [16, 18,19,20,21]; however, LOXL3 involvement in cancer has not been systematically investigated. Our current in silico analyses in different tumor samples uncovered a clear association of LOXL3 expression to melanoma with LOXL3 mRNA being particularly higher among melanomas carrying well-known oncogenic mutations. LOXL3 expression analyses of an ample set of human cell lines confirmed both increased mRNA and protein levels in primary and malignant melanoma samples compared to melanocytes, which were not observed for other lysyl oxidase members. Interestingly, we found that LOXL3 hypomethylation status is associated with LOXL3 upregulation in melanoma tumor samples and cell lines, supporting epigenetic regulation as one mechanism contributing to maintain high LOXL3 expression in this tumor type. Gain and loss-of-function studies have clarified LOXL3 involvement in melanoma and the consequences of LOXL3 deregulation in primary and metastatic melanoma cell lines. Importantly, LOXL3 knockdown was found to be deleterious for cell proliferation in melanoma cells independently of their origin and the oncogenic mutations they harbor. LOXL3-silenced cells ultimately die through apoptosis and ectopic LOXL3 expression collaborates with oncogenic mutations such as BRAFV600E to induce transformation of immortalized human melanocytes, while forced LOXL3 expression in melanoma cells with minimal endogenous LOXL3 enhances their migration and invasion capabilities. Accordingly, tumor growth is delayed in immunocompromised mice orthotopically injected with LOXL3-depleted melanoma cells corroborating LOXL3 involvement in melanomagenesis. Mechanistically, we propose that LOXL3 is responsible for maintaining genome stability since its depletion promotes DNA damage, partial DNA damage checkpoint activation and impaired HR activity. Moreover, LOXL3 silencing leads to a halt in cell proliferation, a delay in cell-cycle progression and defective mitotic completion. The accumulation of DSBs due to LOXL3 knockdown activate the DDR through the ATM pathway, a central player in preventing cells from entering mitosis with damaged or unreplicated DNA . However, the ATR pathway is downregulated in LOXL3-depleted cells, indicating defective activation of the DDR, as demonstrated by the lower HR activity to repair DSB displayed by LOXL3-silenced cells whereas the error-prone NHEJ repair pathway remained unaffected. Importantly, LOXL3 specifically interacts with proteins required to maintain genome integrity (BRCA2, MSH2, NUMA1, SMC1A), some of which are in turn effectors of the DDR (BRCA2, MSH2, SMC1A) . Noticeably, some of these LOXL3 interacting partners are downregulated upon LOXL3 depletion (BRCA2 and MSH2) as well as BRCA1 and Rad51, also required for DNA repair upon damage. Similarly, several essential effectors of mitotic entry and completion like cyclin A, cyclin B1 and AURKA, required for recovery from DNA damage checkpoint activation , are downregulated upon LOXL3 depletion. Moreover, LOXL3-silenced cells struggle to reach and complete mitosis, suffering mitotic catastrophe and apoptosis, or displaying mitotic abnormalities such as chromosome segregation defects and micronuclei appearance. Additionally, LOXL3 interacting protein SMC1A is involved in DSBs repair and is required throughout mitosis to ensure faithful chromosome segregation , whereas NUMA1 coordinates cell-cycle progression and mitotic spindle function to ensure adequate separation of DNA and cell division . While NUMA1 and SMC1A protein levels remain steady upon LOXL3 silencing, we suspect their function might be compromised in the absence of LOXL3 interacting protein thus contributing to faulty mitosis and probably further enhancing DNA damage. LOXL2 interacts with and stabilizes Snail1 by hampering its ubiquitination . In this line, our results suggest that LOXL3 might contribute to maintain functional levels of some of its binding partners such as BRCA2 or MSH2, whereas LOXL3-mediated regulation of NUMA1 and SMC1A function remains to be further explored. We also propose that LOXL3 roles in melanoma are mostly due to the intracellular protein, based on LOXL3 subcellular localization and its biologically relevant association to intracellular partners.
Early in tumorigenesis, melanoma cells most likely escape the DDR checkpoint to enable cell proliferation despite the accumulation of DNA damage, but later on, checkpoint-dependent cell-cycle arrest is required to prevent transformed cells from entering mitotic catastrophe [30, 49]. Melanoma is characterized by a high mutation burden , arising and progressing to malignancy by mechanisms still poorly understood. Our results support that the presence of LOXL3 is required for proper DDR and mitosis in melanoma cells. Upon LOXL3 silencing, melanoma cells fail to activate and execute a competent DDR required for cell survival (illustrated in Fig. 8). Besides, LOXL3 binds to proteins required for mitosis such as NUMA1 and SMC1A while LOXL3 depletion prevents proper mitotic completion. Therefore, we hypothesize that LOXL3 allows melanoma cells to survive in the presence of DNA damage since its depletion results in increased DSBs, genomic aberrations and defective mitosis leading to cell death. To our knowledge, the present data represent the first report demonstrating an involvement for LOXL3 in cancer, particularly establishing melanoma cell addiction to LOXL3, and uncover a novel association between LOXL3 and mechanisms of genome integrity maintenance.
Materials and methods
Public data sets analyses
Analyses regarding mRNA expression, tumor mutational status, sample origin, CNAs, overall number of mutations, and methylation status were performed at http://www.cbioportal.org/ on data of skin cutaneous melanoma (SKCM) patients generated by the TCGA Research Network: http://cancergenome.nih.gov/. LOXL3 accession number used in the analyses was uc002smp.1. For Riker et al.  and Raskin et al.  data sets, the probe of the array corresponding to LOXL3 was 228253_at.
Cell proliferation and cell synchronization
Melanoma cell lines used in the study were obtained in 2013–2014. Hermes cell were kindly provided by D. Bennett (University of London, London, UK), most SK-MEL cell lines by A. Houghton (Memorial Sloan-Kettering Cancer Center, New York, NY), and WM cell lines (as well as 451Lu) were purchased commercially from the Wistar Institute (Philadelphia, PA). FM32 and FM35 melanocytes were kindly provided by M. Soengas, (CNIO, Madrid, Spain) and SB-Cl2 cell line by B. Jiménez (IIB, Madrid, Spain). A375P, A2058, SK-MEL-2, SK-MEL-5, SK-MEL-28, 501MEL and MeWo cells were acquired from ATCC. All experiments were performed within 5–10 passages after thawing and cells were routinely tested for mycoplasma contamination. A375P cells were authenticated by STR profiling. Melanoma cell lines culture and sources are indicated in Supplementary Data and Supplementary Table 1. For cell proliferation assays, 2500–5000 cells/well were seeded in triplicate in 24-well plates, trypsinized and counted at the indicated time intervals. For each time point, total cell number was divided by the number of cells at day 1 post-seeding. For G1/S cell synchronization assays, A375P cells were incubated with 2.5 mM thymidine (Thy, Sigma-Aldrich) for 20 h (single Thy block) or for 18 h with 2 mM Thy, washed with PBS, recovered in complete media for 6–8 h and incubated again for 18 h with 2 mM Thy (double Thy block).
LOXL3 interference and LOXL3 ectopic expression
LOXL3 shRNAs were obtained from the MISSION TRC shRNA human library (Sigma-Aldrich) in pLKO.1-puro vector as well as MISSION pLKO.1-puro non-mammalian shRNA (non-targeting, NTC) control plasmid (SHC002 SIGMA). shRNA scramble control (Crtl) and indicated LOXL3 shRNAs were subsequently cloned into Histone H2B-mRFP1 LV-RFP (Addgene 26001) or Histone H2B-GFP LV-GFP (Addgene 25999), respectively. LOXL3 and LOXL3Δ (lacking exons 4 and 5) FLAG-tagged cDNAs were subcloned into pWPI lentiviral vector (Addgene 12254) that allows simultaneous expression of the transgene and EGFP. Lentiviruses were packaged in HEK293T cells co-transfecting the lentiviral vector, psPAX2 (Addgene 12260) and pMD2.G (Addgene 12259) with Lipofectamine2000 DNA Transfection Reagent (Invitrogen). Melanoma cells were transduced with viral supernatant supplemented with polybrene (2 μg/mL) and either selected with 1 μg/ml puromycin for 2 days (pLKO based lentiviruses) or sorted for EGFP-positive cells using a BD FACSVantage SE cell sorter (pWPI based lentiviruses). For LOXL3 transient silencing, 100 nM of LOXL3 ON-TARGETplus SMARTpool (siSMART-L3) or control scramble siRNA (Dharmacon) were transfected in melanoma cells with Lipofectamine2000 (Invitrogen).
Live-cell imaging analysis
For time-lapse video microscopy, A375P cells expressing Histone H2B-mRFP1 LV-RFP (Crtl) or H2B-GFP LV-GFP (shL3#1 or shL3#2) were synchronized in G1/S after incubation with 2.5 mM thymidine for 20 h and 1.5 × 104 cells were seeded on camera chambers (IBIDI). Mitotic progression was followed by time-lapse microscopy starting 5 h after release in fresh media. Image acquisition was performed using a Cell Observer (Zeiss) equipped with an inverted light and fluorescence microscope and built with an environmental chamber for controlling temperature, humidity, and CO2. Light and fluorescence images were acquired with Plan-APOCHROMAT (40x AN1.3) objective at 5 min intervals and processed using Axiovision Rel. 4.8 software.
Immunoprecipitation and mass spectrometry analysis
Subsequent to cell lysis, immunoprecipitation of FLAG-tagged proteins was performed using FLAG-M2 beads (Sigma-Aldrich) overnight at 4 °C. As a negative control we used cells transduced with an empty vector to hinder unspecific binding to FLAG resin. LOXL3 containing protein complexes were recovered by competitive elution with 3XFLAG peptide (Sigma-Aldrich) and analyzed by mass spectrometry. The MS/MS spectra were searched using the Uniprot human database with LOXL3 and LOXL3Δ sequences inserted using Sequest within Proteome Discoverer (Thermo Fisher). The data was filtered using a 1% false discovery rate searched against a decoy database and only proteins with at least two unique peptides were retained for further analysis. For immunoprecipitation of endogenous proteins, cell lysates were incubated with specific antibodies (Supplementary Table 4) overnight at 4 °C, followed by precipitation with protein G Dynabeads (Invitrogen) at 4 °C for 3 h.
Protocols for annexin V staining, cell-cycle analysis, soft agar, migration, and invasion assays, mouse xenografts, quantitative real-time PCR (qPCR), western blots, immunofluorescence, subcellular fractionation, CRISPR/Cas9 editing, HR and NHEJ activity, DNA methylation and array-CGH are described in the Supplementary Data.
Statistical significance was performed using GraphPad Prism 6. Unless otherwise indicated, numerical data are expressed as mean ± s.e.m. Statistical significance was determined by two-sided Student’s t-test, one-way ANOVA, two-tailed Spearman correlation or Mann–Whitney test. All western blot analyses were independently repeated at least three times, and representative results are shown.
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We thank M. Soengas, M. Malumbres, O. Fernández-Capetillo, S. Mourón (Spanish National Cancer Research Center, CNIO, Spain), B. Jiménez, and B. Sáinz (IIB “Alberto Sols” CSIC-UAM, Spain) for reagents and/or melanoma related material, K. Csiszar (University of Hawaii) for polyclonal LOXL3 antibody, the NYU Proteomics Resource Center (Director, B. Ueberheide) for mass spec analyses, S. Rodríguez (CNIO) for CGH studies, and E. Ballestar (Idibell, Spain) for methylation studies. We also thank D. Navarro (IIB) for help with confocal and video microscopy analysis, L. Molero (SIdI-UAM) for her assistance in cytometry experiments, J. Majuelos for her aid with I.R. and M.J. Mazón (IIB) for advice on differential centrifugation analysis. We would like to specially thank A. Martín and Gema Moreno-Bueno for valuable ideas and members of A.C. lab for helpful discussions. A.F.M. was supported by a FPI predoctoral fellowship from the Spanish Ministry of Economy and Innovation (MINECO) (associated to SAF2013-44739-R) and by Worldwide Cancer Research (16–0295) grant. P.G.S. was a recipient of an Oncology Research Contract (AIO) from the Fundación de la Asociación Española contra el Cáncer (AECC) and is currently funded by Worldwide Cancer Research (16–0295) grant. A.V-N., V.S., S.M., and L.Y. were supported by AICR, MINECO and the Spanish Fondo de Investigaciones Sanitarias (FIS). E.H.'s laboratory is funded by the NIH (US) (R01CA155234, R01CA163891, and R21AR062239). Work in the A.C.'s laboratory is supported by grants from the MINECO (SAF2013-44739-R, SAF2016-76504-R, Consolider-Ingenio ONCOBIO CSD00C-2007-00017), Comunidad de Madrid (S2010/BMD-2303), ISCIII (RTICC-RD12/0036/0007, CIBERONC-CB16/12/00295), all of them partly supported from EU-FEDER funds, and Worldwide Cancer Research UK (formerly AICR, 12-1057, and 16-0295 grants).
Conception and design: P.G.S., A.F., E.H., A.C. Development of methodology: P.G.S., A.F., B.F.-C., A.V.-N. Execution of experiments: P.G.S., A.F., B.F.-C., A.V.-N., V.S., S.M., L.Y., A.G.-G.. Analysis and interpretation of data: P.G.S., A.F., E.H., A.C.. Writing, review, and/or revision of the manuscript: P.G.S., A.F., B.F.-C., H.P., F.P., E.H., A.C. Study supervision: P.G.S., A.F., E.H., A.C.
Conflict of interest
The authors declare that they have no competing interests.
Patricia G. Santamaría and Alfredo Floristán are Co-first authors.
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