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RETRACTED ARTICLE: microRNA-133a exerts tumor suppressive role in oral squamous cell carcinoma through the Notch signaling pathway via downregulation of CTBP2

Cancer Gene Therapy volume 29, pages 62–72 (2022)Cite this article

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This article was retracted on 31 July 2023

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Abstract

microRNAs (miRNAs) have been revealed to participate in some oral cancers and are proved to be effective. In the present study, we tried to explore the biological function of miR-133a in oral squamous cell carcinoma (OSCC) cells. The relationship that C-terminal-binding proteins 2 (CTBP2) was the putative target gene of miR-133a revealed from bioinformatics analysis was further was further validated by dual-luciferase reporter gene assay. In total, 40 patients with OSCC were enrolled for characterization of miR-133a, CTBP2, and Notch signaling pathway-related gene expression in clinical OSCC tissues. Low expression of miR-133a and high expression of CTBP2, Hes1, Notch-1, and Notch-3 were determined in OSCC tissues. OSCC cell lines were transfected with miR-133a inhibitor, miR-133a mimic, or shRNA targeting CTBP2, in response to which cell proliferation, migration, invasion, cell cycle, and apoptosis were evaluated. Transfection of miR-133a mimic induced apoptosis and inhibited OSCC cell proliferation, migration, and invasion and this was demonstrated to be attributable to decreased CTBP2 expression and suppression of the Notch signaling pathway. Taken together, we concluded that miR-133a acted as a tumor suppressor in OSCC through inhibition of the Notch signaling pathway via binding to CTBP2.

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Fig. 1: CTBP2 and hsa-miR-133a are putative regulators in OSCC.
Fig. 2: CTBP2 was the target gene of miR-133a.
Fig. 3: Expression of CTBP2 and Notch signaling pathway-related factors were found high in OSCC tissues, while expression of miR-133a had opposite trend.
Fig. 4: MiR-133a can regulate the activation of notch pathway through CTBP2.
Fig. 5: Upregulated miR-133a inhibited the proliferation and the colony formation capacity of OSCC cells.
Fig. 6: Overexpression of miR-133a could effectively inhibit the invasion and migration of OSCC cells.
Fig. 7: Overexpression of miR-133a promoted cell cycle transition from G/G 1 to S phase.
Fig. 8: MiR-133a promoted apoptosis by negatively regulating of CTBP2.
Fig. 9: Model of molecular mechanisms involved in miR-133a regulation of OSCC cells.

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Authors and Affiliations

  1. Department of Stomatology, Linyi People’s Hospital, 276000, Linyi, P. R. China

    Wei Liu, Xiaoming Shi & Baoliang Wang

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  1. Wei Liu
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Correspondence to Baoliang Wang.

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The study was approved by the Institutional Review Board of Linyi People’s Hospital and in accordance with the declaration of Helsinki. Written informed consent was obtained from each participant.

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This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1038/s41417-023-00649-4

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Liu, W., Shi, X. & Wang, B. RETRACTED ARTICLE: microRNA-133a exerts tumor suppressive role in oral squamous cell carcinoma through the Notch signaling pathway via downregulation of CTBP2. Cancer Gene Ther 29, 62–72 (2022). https://doi.org/10.1038/s41417-020-00289-y

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