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Long non-coding RNA DLEU1 predicts poor prognosis of gastric cancer and contributes to cell proliferation by epigenetically suppressing KLF2

Cancer Gene Therapy volume 25, pages 58–67 (2018)Cite this article

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A Correction to this article was published on 23 August 2021

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Abstract

Currently, accumulating documents have paid great attention to the critical role of long non-coding RNAs. The long non-coding RNAs DLEU1 has been demonstrated to be dysregulated in many solid tumors and hematological malignancies. However, the detailed descriptions about its potential roles and molecular mechanism in gastric cancer (GC) are still blurry. As for our research, it was found out that DLEU1 was observably intensified in GC tissues and cell lines. And highly expressed DLEU1 was relevant to tumor size, advanced stage of pathology and lymph node metastasis in GC patients. Silenced DLEU1 obviously suppressed proliferation via leading to the cell cycle arrest and inducing cell apoptosis of GC. Furthermore, mechanistic experiments uncovered that DLEU1 could recruit LSD1 (lysine specific demethylase 1) to the promoter regions of KLF2 and then suppressed its transcription. In addition, rescue assays revealed that the oncogenic function mediated by DLEU1 in GC was partly by regulating KLF2. Collectively, our findings manifested that DLEU1 might serve as an oncogene in GC.

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Fig. 1: DLEU1 is highly expressed in human GC tissues and cell lines
Fig. 2
Fig. 3: Silenced DLEU1 retards cell proliferation and induces apoptosis
Fig. 4: DLEU1 epigenetically silences KLF2 transcription by interacting with LSD1.
Fig. 5: The oncogenic role of DLEU1 in GC is in KLF2-dependent manner

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Acknowledgements

Sincere thanks from the authors are conveyed to the laboratory members.

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  1. Xiaobin Li and Zongze Li contributed equally to this manuscript.

Authors and Affiliations

  1. Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences&Peking Union Medical College, Beijing, 100730, P. R. China

    Xiaobin Li, Zongze Li, Ziwen Liu, Jianchun Xiao, Shuting Yu & Yimin Song

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Correspondence to Yimin Song.

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The original online version of this article was revised: After publication it was discovered that there was an error in Figure 3E.

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Li, X., Li, Z., Liu, Z. et al. Long non-coding RNA DLEU1 predicts poor prognosis of gastric cancer and contributes to cell proliferation by epigenetically suppressing KLF2. Cancer Gene Ther 25, 58–67 (2018). https://doi.org/10.1038/s41417-017-0007-9

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