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Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nature Protocols volume 2, pages 38–41 (2007)Cite this article

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Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

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Change history

  • 04 December 2008

    In the version of this article initially published, in the Reagent Setup section on p. 39, the recipe for lithium acetate (1.0 M) called for 102 g of lithium acetate dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.

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Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, R3E 0W3, Manitoba, Canada

    R Daniel Gietz

  2. Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, 90095, California, USA

    Robert H Schiestl

Authors
  1. R Daniel Gietz
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  2. Robert H Schiestl
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Correspondence to Robert H Schiestl.

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Gietz, R., Schiestl, R. Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 38–41 (2007). https://doi.org/10.1038/nprot.2007.15

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