Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Protocol
  • Published:

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nature Protocols volume 2, pages 31–34 (2007)Cite this article

This article has been updated

Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Change history

  • 20 November 2008

    Note: In the version of this article initially published, in the Reagent Setup section on p. 32, the recipe for lithium acetate (1.0 M) called for 102 g of lithium acetate dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.

References

  1. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163–168 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Schiestl, R.H. & Gietz, R.D. High efficiency transformation of intact yeast cells using single-stranded nucleic acids as carrier. Curr. Genet. 16, 339–346 (1989).

    Article  CAS  PubMed  Google Scholar 

  3. Gietz, R.D., Schiestl, R.H., Willems, A.R. & Woods, R.A. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11, 355–360 (1995).

    Article  CAS  PubMed  Google Scholar 

  4. Gietz, R.D. & Woods, R.A. Yeast transformation. in Methods in Enzymology, Guide to Yeast Genetics and Cell Biology, Parts B and C, Vol. 350 (eds. Guthrie, C. & Fink, G.R.) 87–96 (Academic Press, San Diego, CA, 2001).

    Google Scholar 

  5. Linske-O'Connell, L.I., Sherman, F. & McLendon, G. Stabilizing amino acid replacements at position 52 in yeast iso-1-cytochrome c: in vivo and in vitro effects. Biochemistry 34, 7094–7102 (1995).

    Article  CAS  PubMed  Google Scholar 

  6. Yamamoto, T., Moerschell, L.R.P., Wakem, P., Ferguson, D. & Sherman, F. Parameters affecting the frequencies of transformation and co-transformation with synthetic oligonucleotides in yeast. Yeast 8, 935–948 (1992).

    Article  CAS  PubMed  Google Scholar 

  7. Gietz, R.D. & Woods, R.A. Genetic transformation of yeast. BioTechniques 30, 816–831 (2001).

    Article  CAS  PubMed  Google Scholar 

  8. Gietz, R.D. & Schiestl, R.H. Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/nprot.2007.14.

  9. Gietz, R.D. & Schiestl, R.H. Large scale high efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/nprot.2007.15.

  10. Gietz, R.D. & Schiestl, R.H. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/nprot.2007.16.

  11. Rose, M.D. Isolation of genes by complementation in yeast. Methods Enzymol. 152, 481–504 (1987).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, R3E 0W3, Manitoba, Canada

    R Daniel Gietz

  2. Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, 90095, California, USA

    Robert H Schiestl

Authors
  1. R Daniel Gietz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert H Schiestl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Robert H Schiestl.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gietz, R., Schiestl, R. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 31–34 (2007). https://doi.org/10.1038/nprot.2007.13

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nprot.2007.13

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing