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Characterization of a newly discovered T-cell receptor β-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment

Nature Medicine volume 6, pages 904–909 (2000)Cite this article

Abstract

The facilitating cell is a rare CD8+ bone marrow subpopulation that can enhance allogeneic hematopoietic stem cell engraftment across complete major histocompatibility complex barriers without inducing acute graft-versus-host disease. Here we describe a CD3ɛ-associated complex on the facilitating cell surface that consists of the T-cell receptor β-chain disulfide-linked to a previously unknown 33-kilodalton glycoprotein. Provisionally called FCp33, this glycoprotein does not represent any of the known protein chains or surrogates associated with CD3–T-cell receptor β. Expression of this CD3–T-cell receptor β–FCp33 complex directly correlates with the facilitating cell's functional ability to enhance allogeneic stem cell engraftment in vivo.

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Figure 1: Flow cytometric analysis of facilitating cells and splenic T cells.
Figure 2: Initial characterization of the FCp33 complex.
Figure 3: Biotin western blots of serial immunoprecipitation experiments.
Figure 4: Biochemical characterization of FCp33.
Figure 5: Correlation of CD3–TCR-β–FCp33 expression with facilitation of allogeneic SC engraftment.

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Acknowledgements

We thank R. Lakomy and the Pittsburgh Cancer Institute Flow Cytometry Facility for cell sorting support, as well as M. Mazzocchetti and the Animal Care Facility for animal care. We thank C.L. Kaufman and S.T. Ildstad for their support and R.L. Simmons for reviewing the manuscript. This study was supported in part by the American Society of Transplant Surgeons–Roche Surgical Scientist Scholarship, American College of Surgeons Resident Scholarship, Leukemia Society of America Special Fellow Award, and National Institutes of Health grant R29-AI4093.

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Authors and Affiliations

  1. Department of Surgery, University of Pittsburgh Medical Center Health System, University of Pittsburgh, Pittsburgh, 15261, Pennsylvania, USA

    Matthew J. Schuchert

  2. Division of Thoracic Surgery, and Department of Pediatric Oncology, Brigham and Women's Hospital Harvard Medical School, Dana Farber Cancer Institute, Boston, 02115 , Massachusetts, USA

    Renee D. Wright & Yolonda L. Colson

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Correspondence to Yolonda L. Colson.

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Schuchert, M., Wright, R. & Colson, Y. Characterization of a newly discovered T-cell receptor β-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med 6, 904–909 (2000). https://doi.org/10.1038/78667

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