Letter | Published:

Bidirectional electromagnetic control of the hypothalamus regulates feeding and metabolism

Nature volume 531, pages 647650 (31 March 2016) | Download Citation

Abstract

Targeted, temporally regulated neural modulation is invaluable in determining the physiological roles of specific neural populations or circuits. Here we describe a system for non-invasive, temporal activation or inhibition of neuronal activity in vivo and its use to study central nervous system control of glucose homeostasis and feeding in mice. We are able to induce neuronal activation remotely using radio waves or magnetic fields via Cre-dependent expression of a GFP-tagged ferritin fusion protein tethered to the cation-conducting transient receptor potential vanilloid 1 (TRPV1) by a camelid anti-GFP antibody (anti-GFP–TRPV1)1. Neuronal inhibition via the same stimuli is achieved by mutating the TRPV1 pore, rendering the channel chloride-permeable. These constructs were targeted to glucose-sensing neurons in the ventromedial hypothalamus in glucokinase–Cre mice, which express Cre in glucose-sensing neurons2. Acute activation of glucose-sensing neurons in this region increases plasma glucose and glucagon, lowers insulin levels and stimulates feeding, while inhibition reduces blood glucose, raises insulin levels and suppresses feeding. These results suggest that pancreatic hormones function as an effector mechanism of central nervous system circuits controlling blood glucose and behaviour. The method we employ obviates the need for permanent implants and could potentially be applied to study other neural processes or used to regulate other, even dispersed, cell types.

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Acknowledgements

We would like to thank A. North, P. Ariel and K. Thomas for help with confocal imaging, D. Acehan and K. Uryu for performing EM studies and S. Korres for assistance with the manuscript. This work was funded by Howard Hughes Medical Institute, the JPB Foundation, the National Institutes of Health (GM095654 and MH105941) and a Rensselaer Fellowship (to J.S.) under an NIH predoctoral training grant (GM067545). Support for this project was provided by a grant from the Robertson Foundation.

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Affiliations

  1. Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10065, USA

    • Sarah A. Stanley
    • , Leah Kelly
    • , Kaamashri N. Latcha
    • , Sarah F. Schmidt
    • , Xiaofei Yu
    • , Alexander R. Nectow
    •  & Jeffrey M. Friedman
  2. Department of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA

    • Jeremy Sauer
    •  & Jonathan S. Dordick
  3. Department of Radiology, Weill Cornell Medical College, New York, New York 10065, USA

    • Jonathan P. Dyke
  4. Howard Hughes Medical Institute, New York, New York 10065, USA

    • Jeffrey M. Friedman

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Contributions

J.M.F. conceived the project and supervised the studies. S.A.S. and L.K. designed and performed the experiments. K.L. and S.F.S. provided technical assistance. A.N. assisted with optogenetic studies, J.S. assisted with magnet activation studies and X.Y. assisted with cell culture studies. J.D. provided technical advice for in vivo magnet activation studies. J.S.D. provided technical advice. S.A.S., L.K. and J.M.F. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jeffrey M. Friedman.

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https://doi.org/10.1038/nature17183

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