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A transfection method of PS-asODNs targeting ANGPTL4 in multicellular structures of hepatocarcinoma cell line

Cancer Gene Therapy volume 22, pages 285–290 (2015)Cite this article

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Abstract

To find an efficient transfection method for metastatic cancer cells, we established a three-dimensional (3D) growth model for solid tumor cells to mimic the metastatic cancer cells in the vascular system and compared the efficiency of several transfection methods in vitro. We found that it was optimal to transfect two-dimensional cells in vitro and detach them for 3D growth 6 h later. The transfection efficiency of this method was high, and the results were reliable. This method can be used to deliver several types of small molecules into the 3D metastatic cell model. Using this method, we increased our understanding of why drugs that are effective in vitro cannot treat the disease in vivo. If this phenomenon occurs due to the resistance of the cells to the drug, other treatment agents for the disease must be identified. However, if this occurs because the agent cannot reach the cells inside the 3D aggregate, we can improve the delivery efficiency by using methods that target the agent to all cells. Briefly, the method introduced in this study will contribute to future research focusing on the 3D metastatic cell model as well as on drug development for various solid tumors.

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Acknowledgements

The authors gratefully acknowledge Professor Qihong Huang (The Wistar Institute, University of Pennsylvania, Philadelphia, PA, USA) and Editors Benjamin G, Adam P and Elizabeth G (American Journal Experts, Durham, NC, USA) for their critically constructive suggestions regarding our manuscript. This work was supported by grants from the Natural Science Foundation of China (30700357, 30772031 and 30873025), the Natural Science Foundation of Shandong Province (ZR2010HM033) and Science and Technology Development of Shandong Province (2013YD18016).

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  1. Q Kong and G Wu: These authors contributed equally to this work.

Authors and Affiliations

  1. TaiShan Medical University, Taian, China

    Q Kong

  2. Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China

    Q Kong, G Wu, J Du & L Cao

  3. Department of Immunology, School of Medicine, Shandong University, Jinan, China

    L Han & W Sun

  4. Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan, China

    Z Zhang

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  1. Q Kong
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  2. G Wu
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Correspondence to L Cao.

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Kong, Q., Wu, G., Han, L. et al. A transfection method of PS-asODNs targeting ANGPTL4 in multicellular structures of hepatocarcinoma cell line. Cancer Gene Ther 22, 285–290 (2015). https://doi.org/10.1038/cgt.2015.22

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