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Electroporation markedly improves Sleeping Beauty transposon-induced tumorigenesis in mice

Cancer Gene Therapy volume 21, pages 333–339 (2014)Cite this article

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Abstract

The Sleeping Beauty (SB) transposon system is an important tool for genetic studies. It is used to insert a gene of interest into the host chromosome, thus enabling permanent gene expression. However, this system is less useful in higher eukaryotes because the transposition frequency is low. Efforts to improve the efficacy of the SB transposon system have focused on the method of gene delivery, but although electroporation has recently attracted much attention as an in vivo gene delivery tool, the simultaneous use of electroporation and the SB transposon system has not been studied for gene transfer in mice. In this study, electroporation was used in a model of SB transposon-induced insertional tumorigenesis. Electroporation increased the rate of tumor development to three times that of the control group. There was no difference in phenotype between tumors induced with the SB transposon system alone and those induced by the SB transposon and electroporation. Electroporation therefore may be an efficient means of improving the efficacy of gene transfer via the SB transposon system.

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Acknowledgements

This study was supported by a grant from the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI06C0868 and HI10C2014). VGX International kindly provided Cellectra (the electroporation device).

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Authors and Affiliations

  1. Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea

    S Jung, H-J Choi, H-K Park, W Jo, S Jang, J-E Ryu, E-S Yu & W-C Son

  2. Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

    S Jung, H-J Choi, H-K Park, W Jo, S Jang, J-E Ryu, W-J Kim, E-S Yu & W-C Son

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  1. S Jung
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Correspondence to W-C Son.

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Jung, S., Choi, HJ., Park, HK. et al. Electroporation markedly improves Sleeping Beauty transposon-induced tumorigenesis in mice. Cancer Gene Ther 21, 333–339 (2014). https://doi.org/10.1038/cgt.2014.33

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