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Tissue ablation by a free-electron laser tuned to the amide II band

Nature volume 371, pages 416–419 (1994)Cite this article

Abstract

EFFORTS to ablate soft tissue with conventional lasers have been limited by collateral damage and by concern over potential photochemical effects1–5. Motivated by the thermal-confinement model6, past infrared investigations targeted the OH-stretch mode of water with fast pulses from lasers emitting near 3,000 nm (refs 1, 7–9). What does a free-electron laser offer for the investigation of tissue ablation? Operating at non-photochemical single-photon energies, these infrared sources can produce trains of picosecond pulses tunable to the vibrational modes of proteins, lipids and/or water. We report here that targeting free-electron laser radiation to the amide II band of proteins leads to tissue ablation characterized by minimal collateral damage while maintaining a substantial ablation rate. To account for these observations we propose a novel ablation mechanism based on compromising tissue through resonant denaturation of structural proteins.

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References

  1. Srinivasan, R. Science 234, 559–565 (1986).

    Article  ADS  CAS  Google Scholar 

  2. Alfano, R. R. & Doukas, A. G. (eds) IEEE J. quant. Electron. QE-20, 1342–1532 (1984).

  3. Puliafito, C. A., Steinert, R. F. & Deutsch, T. F. Ophthalmology 92 (6), 741–748 (1985).

    Article  CAS  Google Scholar 

  4. Robertson J. H. & Clark, W. C. (eds) Lasers in Neurosurgery (Kluwer Academic, Dordrecht, 1988).

    Google Scholar 

  5. Anderson, R. R. & Parrish, J. A. Science 220, 524–527 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Wolbarsht, M. L. IEEE J. quant. Electron. QE-20, 1427–1432 (1984).

  7. Zysset, B., Fujimoto, J. G., Puliafito, C. A., Birngruber, R. & Deutsch, T. F. Lasers Surg. Med. 9, 193–204 (1989).

    Article  CAS  Google Scholar 

  8. Walsh, J. T. Jr, Flotte, T. J. & Deutsch, T. F. Lasers Surg. in Med. 9, 314–326 (1989).

    Article  Google Scholar 

  9. Stern, D., Puliafito, C. A., Dobi, E. T. & Reidy, W. T. Ophthalmology 95 (10), 1434–1441 (1988).

    Article  CAS  Google Scholar 

  10. Madey, J. M. J. J. appl. Phys. 42, 1906–1930 (1971).

    Article  ADS  CAS  Google Scholar 

  11. Brau, C. A. Science 239, 1115–1121 (1988).

    Article  ADS  CAS  Google Scholar 

  12. Dlott, D. & Fayer, M. J. Opt. Soc. Am. B 6 (5), 977–994 (1989).

    Article  ADS  CAS  Google Scholar 

  13. Edwards, G. S., Johnson, B., Kozub, J., Tribble, J. & Wagner, K. Opt. Engng 32 (2), 314–319 (1993).

    Article  ADS  Google Scholar 

  14. Austin, R. H., Roberson, M. W. & Mansky, P. Phys. Rev. Lett. 62, 1912–1915 (1989).

    Article  ADS  CAS  Google Scholar 

  15. Fann, W. et al. Phys. Rev. Lett. 64, 607–610 (1990).

    Article  ADS  CAS  Google Scholar 

  16. Zimdars, D. et al. Phys. Rev. Lett. 70, 2718–2721 (1993).

    Article  ADS  CAS  Google Scholar 

  17. Tuncel, E. et al. Phys. Rev. Lett. 70, 4146–4149 (1993).

    Article  ADS  CAS  Google Scholar 

  18. Brau, C. A. Nucl. Instrum. Meth. A319, 47–50 (1992).

    Article  ADS  CAS  Google Scholar 

  19. Graener, H., Seifert, G. & Laubereau, A. Phys. Rev. Lett. 66, 2092–2095 (1991).

    Article  ADS  CAS  Google Scholar 

  20. Weissbluth, M. Photon-Atom Interactions Ch. 7, p. 376 (Academic, Boston, 1989).

    Google Scholar 

  21. Brewer, R. G. & Rieckhoff, K. E. Phys. Rev. Lett. 13, 334–336 (1964).

    Article  ADS  Google Scholar 

  22. Berman, E. R. Biochemistry of the Eye (Plenum, New York, 1991).

    Book  Google Scholar 

  23. Liebman, S. A. & Levy, E. J. (eds) Pyrolysis and GC in Polymer Analysis (Dekker, New York, 1984).

  24. Joly, M. A Physico-Chemical Approach to the Denaturation of Proteins, 153 (Academic, London, 1965).

    Google Scholar 

  25. Flory, P. J. Statistical Mechanics of Chain Molecules Ch. 7. Section 6 (Hanser, New York, 1969).

    Book  Google Scholar 

  26. Privalov, P. R. et al. J. molec. Biol. 205, 737–750 (1989).

    Article  CAS  Google Scholar 

  27. McClain, P. E. & Wiley, E. R. J. biol. Chem. 247 (3), 692–697 (1972).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee, 37235, USA

    Glenn Edwards, Lou Reinisch, Bruce Johnson, Marcus Mendenhall & Jerri Tribble

  2. Departments of Ophthalmology and Visual Sciences,

    Regan Logan & Denis O'Day

  3. Neurosurgery,

    Michael Copeland

  4. Otolaryngology,

    Glenn Edwards, Lou Reinisch, Robert Maciunas, Robert Ossoff & Jay Werkhaven

  5. Pathology, Vanderbilt University Medical School, Nashville, Tennessee, 37232, USA

    Jeffrey Davidson

Authors
  1. Glenn Edwards
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  2. Regan Logan
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  3. Michael Copeland
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  4. Lou Reinisch
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  5. Jeffrey Davidson
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  6. Bruce Johnson
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  7. Robert Maciunas
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  8. Marcus Mendenhall
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  9. Robert Ossoff
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  10. Jerri Tribble
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Edwards, G., Logan, R., Copeland, M. et al. Tissue ablation by a free-electron laser tuned to the amide II band. Nature 371, 416–419 (1994). https://doi.org/10.1038/371416a0

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