Abstract
Fischer rat 3T3 (FR3T3) fibroblasts transfected with a cellular myc gene1 can be induced to grow and form colonies in soft agar by treatment either with epidermal growth factor (EGF) alone or with the combination of platelet-derived growth factor (PDGF) and type-β transforming growth factor (TGF-β)2. We now show that induction of anchorage-independent growth by each of these sets of growth factors involves different cellular pathways which can be distinguished by their sensitivity to retinoic acid. Colony formation induced by the combined action of PDGF and TGF-β is 100-fold more sensitive to inhibition by retinoic acid than is colony formation induced by treatment of the myc-transfected cells with EGF. Moreover, retinoic acid (10−8 M) is inhibitory for colony growth whenever TGF-β is present, regardless of whether the effects of TGF-β are stimulatory, as occurs in the presence of PDGF, or inhibitory, as found in the presence of EGF2.
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Roberts, A., Roche, N. & Sporn, M. Selective inhibition of the anchorage-independent growth of myc-transfected fibroblasts by retinoic acid. Nature 315, 237–239 (1985). https://doi.org/10.1038/315237a0
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DOI: https://doi.org/10.1038/315237a0
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