Abstract
Platelet-derived growth factor1–4 (PDGF) is known to be involved in regulating the mitosis of connective tissue cells, and recent studies have also shown that it may function in mediating cellular transformation5–8. The oncogene carried by simian sarcoma virus, sis, is homologous to one chain of PDGF5–8, and treatment of non-neoplastic cells with this growth factor results in increased transcription of another oncogene, myc (ref. 9). PDGF also stimulates the synthesis of proteins that are characteristic of transformed cells10. However, phenotypic transformation does not appear to result from the action of PDGF alone. For example, expression of myc does not transform cells in the absence of other oncogene expression11. We have recently shown that platelets contain another peptide growth factor, transforming growth factor-β (TGF-β)12,13, in addition to PDGF. We report here that extracts of human platelets can induce anchorage-independent growth of non-neoplastic rat kidney (NRK) fibroblasts, but that purified PDGF alone does not elicit this effect. Rather, the transforming activity of the platelet extract is due to a concerted action of three distinct peptides: PDGF, TGF-β and a newly identified analogue of epidermal growth factor (EGF).
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Assoian, R., Grotendorst, G., Miller, D. et al. Cellular transformation by coordinated action of three peptide growth factors from human platelets. Nature 309, 804–806 (1984). https://doi.org/10.1038/309804a0
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DOI: https://doi.org/10.1038/309804a0
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