Abstract
More than 300,000 copies of the ∼300 base pair (bp)-long AluI DNA sequence are dispersed throughout the human genome1–7. Several AluI sequences have been shown to contain sequences which promote transcription in vitro by the enzyme RNA polymerase III8–11. While the function(s) of transcripts synthesized from such promoters is unclear, one notion is that RNA polymerase III transcription from an AluI promoter might have an effect on expression of a nearby RNA polymerase II gene, specifically, the β-globin gene (see Fig. 1). However, we found that this AluI promoter is expressed efficiently in vitro only in conditions dramatically different from those required for the β-globin and other promoters (both RNA polymerases II and III). Surprisingly, the major transcript produced from this promoter is 2.3 kilobases (kb) long, much longer than any RNA polymerase III transcript described previously.
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Manley, J., Colozzo, M. Synthesis in vitro of an exceptionally long RNA transcript promoted by an AluI sequence. Nature 300, 376–379 (1982). https://doi.org/10.1038/300376a0
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DOI: https://doi.org/10.1038/300376a0
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