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Compounds interfering in the Diphenylamine Test for Deoxyribonucleic Acid

Abstract

THE use of DNA (deoxyribonucleic acid) as a reference standard in biochemical investigations1, which is now well established, rests on the observed constancy of DNA content in each somatic cell in a species. With certain exceptions, the number of cells in a sample may be related to the amount of DNA2.

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References

  1. Hinshelwood, C., and Caldwell, M. L., J. Chem. Soc., 1415 (1950). Sevag, M. G., Smolens, J., and Lackman, D. B., J. Biol. Chem., 134, 523 (1940). Seibert, E. A., ibid., 133, 593 (1940). Hoagland, M. B., J. Exp. Med., 72, 139 (1940).

    Google Scholar 

  2. The occurrence of polyploidy or of polynuclear cells, for example, will upset this relationship; for example, see The Nucleic Acids, edit. by Chargaff, E., and Davidson, J. N., 1 (Academic Press, Inc., New York, 1955).

  3. Dische, Z., Mikrochemie, 8, 4 (1930).

    Article  CAS  Google Scholar 

  4. Dische, Z., in The Nucleic Acids, edit. by Chargaff, E., and Davidson J. N., 1 (Academic Press, Inc., New York, 1955).

    Google Scholar 

  5. Davidson, J. N., The Biochemistry of the Nucleic Acids, 60 (Methuen Monograph, London, 1950). Pirie, N. W., Brit. J. Exp. Path., 17, 405 (1936).

    Google Scholar 

  6. Ayala, L., Moore, S., and Hess, K., J. Clin. Invest., 30, 781 (1951). Ayala, L., Hahn, F. E., and Hess, K., Proc. Soc. Exp. Biol. Med., 91, 528 (1952).

    Article  CAS  Google Scholar 

  7. Woodhouse, H., Brit. J. Cancer, 3, 510 (1949).

    Article  CAS  Google Scholar 

  8. Hoagland, M. B., J. Exp. Med., 72, 139 (1940). Hinshelwood, C., loc. cit

    Article  CAS  Google Scholar 

  9. Chargaff, E., Vischer, E., Doniger, R., Green, C., and Mitani, S., J. Biol. Chem., 177, 405 (1949). Feulgen, R., Z. physiol. Chem., 128, 154 (1923); 135, 203 (1924). See also Di Stefano, J., Proc. U.S. Nat. Acad. Sci., 34, 75 (1948).

    CAS  PubMed  Google Scholar 

  10. Li, C. H., Overend, W. G., and Stacey, M., Nature, 163, 538 (1949). DiStefano, J., loc. cit. Danielli, J. F., Symp. Soc. Exp. Biol., 1, 101 (1947); Quart. J. Micro. Sci., 90, 67 (1949). Stedman, R. J., and Stedman, M., Symp. Soc. Exp. Biol., 1, 232 (1947).

    Article  ADS  CAS  Google Scholar 

  11. Pigman, W., The Carbohydrates (Academic Press, Inc., New York, 1957).

    Google Scholar 

  12. Ellet, J., and Tollens, B., Ber., 38, 492 (1905).

    Article  Google Scholar 

  13. Pummerer, R., and Gump, W., Ber., 56, 999 (1923).

    Google Scholar 

  14. Zilliken, F., and Whitehouse, M. W., Adv. in Carbohydrate Chem., 13, 237 (1958); Blix, G., Z. physiol. Chem., 240, 43 (1936); Hiyama, N., Tohoku J. Exp. Med., 51, 319 (1948).

    CAS  Google Scholar 

  15. Klenk, E., Z. physiol. Chem., 268, 50 (1941); 273, 76 (1942).

    Article  CAS  Google Scholar 

  16. Barry, V. C., Nature, 179, 206 (1957).

    Article  ADS  CAS  Google Scholar 

  17. Zilliken, F., and Whitehouse, M. W., loc. cit.;, Kuhn, R., and Brossmer, R., Angew. Chem., 70, 25 (1958).

    Google Scholar 

  18. Ciba Symp. “Mucopolysaccharides”, 298 (1958).

  19. Fearnley, G., et al., Ann. Rheum. Dis., 14, 226 (1955); Niazzi, S., and State, D., Cancer Res., 8, 653 (1948); Pirie, N. W., et al., loc. cit.; Ayala, L., et al., loc. cit.;

    Article  CAS  Google Scholar 

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LEE, J. Compounds interfering in the Diphenylamine Test for Deoxyribonucleic Acid. Nature 200, 264–266 (1963). https://doi.org/10.1038/200264a0

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