Letter | Published:

Action of Insulin on the Isolated Rat Diaphragm in the Presence of Actinomycin D and Puromycin

Naturevolume 199pages11831184 (1963) | Download Citation



WITHIN recent years, conclusive evidence has been obtained of a direct stimulation of transport of sugar from the extracellular to the intracellular space of the isolated perfused heart of the rat1 and the isolated ‘caged’ diaphragm of the rat2. It is, however, difficult to explain the entire mechanism of insulin action through this effect, for clear-cut insulin-induced stimulations have been observed in the absence of extracellular or intracellular glucose: these effects include biosynthesis of proteins from labelled precursors3, increased labelling of RNA from 14C-adenine or glucose4 and increased specific labelling of high-energy phosphates from intracellular inorganic phosphate labelled with phosphorus-32 (ref. 5). This evidence raised the question4 whether an induced biosynthesis of messenger RNA (ref. 6), which could in turn stimulate the synthesis of specific enzymatic proteins, may participate in the hormonal action; recent work on the effects of other hormones7,8 supports this hypothesis.

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    Manchester, K. L., Biochem. J., 81, 135 (1961).

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    Wool, I. G., Biochim. Biophys. Acta, 68, 28 (1963).

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    Clauser, H., Volfin, P., and Eboué-Bonis, D., Gen. Comp. Endocrinol., 2, 369 (1962).

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    Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

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    Mueller, G. C., Gorski, J., and Aizawa, Y., Proc. U.S. Nat. Acad. Sci., 47, 164 (1961).

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    Cadenas, E., Kaji, H., Park, C. R., and Rasmussen, H., J. Biol. Chem., 236, PC 63 (1961).

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    Volfin, P., Eboué-Bonis, D., Chambaut, A. M., and Clauser, H., Bull. Soc. Chim. Biol., 45, 458 (1963).

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  1. Laboratoire de Chimie Biologique, Faculté des Sciences, 96 Boulevard Raspail, Paris

    • , A. M. CHAMBAUT
    • , P. VOLFIN
    •  & H. CLAUSER


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