Relation of Invasive Capacity to Passage of Lymphocytic Cells through Cellulose Membrane Filters

Abstract

IN a previous paper¹ it was reported that when three lymphocytic tumours, each with a different capacity to invade host tissues, were grown in Millipore Type AA filters (pore size 0.8 ± 0.05µ) (Millipore Filter Corporation, Bedford, Mass.) in double diffusion chambers, the cells of the tumours were unable to penetrate the filter after growing for 60 days in an isologous host. More recent experiments with this filter have shown that while the cells of these tumours are able to pass through the AA filter, there is nevertheless a striking difference in the capacity of the cells of an invasive and a non-invasive tumour to penetrate the pores of this membrane and establish a growing cell population on the other side.