A bacterial enzyme that uses guide RNA molecules to target DNA for cleavage has been adopted as a programmable tool to site-specifically modify genomes of cells and organisms, from bacteria and human cells to whole zebrafish.
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References
Ishino, Y., Shinagawa, H., Makino, K., Amemura, M. & Nakata, A. J. Bacteriol. 169, 5429–5433 (1987).
Cong, L. et al. Science 339, 819–823 (2013).
Mali, P. et al. Science 339, 823–826 (2013).
Cho, S. W., Kim, S., Kim, J. M. & Kim, J. S. Nature Biotechnol. 31, 230–232 http://dx.doi.org/10.1038/nbt.2507 (2013).10.1038/nbt.2507
Hwang, W. Y. et al. Nature Biotechnol. 31, 227–229 http://dx.doi.org/10.1038/nbt.2501 (2013).10.1038/nbt.2501
Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L. A. Nature Biotechnol. 31, 233–239 http://dx.doi.org/10.1038/nbt.2508 (2013).10.1038/nbt.2508
Jinek, M. et al. Science 337, 816–821 (2012).
Jansen, R., Embden, J. D., Gaastra, W. & Schouls, L. M. Mol. Microbiol. 43, 1565–1575 (2002).
Haft, D. H., Selengut, J., Mongodin, E. F. & Nelson, K. E. PLoS Comput. Biol. 1, e60 (2005).
Mojica, F. J., Diez-Villasenor, C., Garcia-Martinez, J. & Soria, E. J. Mol. Evol. 60, 174–182 (2005).
Pourcel, C., Salvignol, G. & Vergnaud, G. Microbiology 151, 653–663 (2005).
Makarova, K. S., Grishin, N. V., Shabalina, S. A., Wolf, Y. I. & Koonin, E. V. Biol. Direct 1, 7 (2006).
Barrangou, R. et al. Science 315, 1709–1712 (2007).
Brouns, S. J. et al. Science 321, 960–964 (2008).
Deltcheva, E. et al. Nature 471, 602–607 (2011).
Garneau, J. E. et al. Nature 468, 67–71 (2010).
Sapranauskas, R. et al. Nucleic Acids Res. 39, 9275–9282 (2011).
Carroll, D. Mol. Ther. 20, 1658–1660 (2012).
Jinek, M. et al. eLIFE http://dx.doi.org/10.7554/eLife.00471 (2013).
Qi, L. S. et al. Cell 152, 1173–1183 (2013).
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Charpentier, E., Doudna, J. Rewriting a genome. Nature 495, 50–51 (2013). https://doi.org/10.1038/495050a
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DOI: https://doi.org/10.1038/495050a
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