Oligonucleotide probes articles within Nature Chemistry

Featured

• Article | 26 October 2023

  Heteromultivalency enables enhanced detection of nucleic acid mutations

  Detecting genetic mutations, such as single nucleotide polymorphisms (SNPs), is essential for disease diagnostics but can be difficult using homomultivalent DNA hybridization-based approaches. Now, heteromultivalent hybridization is used to fine-tune binding specificity for the detection of one or two SNPs in a single target, enabling straightforward discrimination between adjacent and distant mutations and different viral strains.

  • Brendan R. Deal
  • , Rong Ma
  •  & Khalid Salaita

• Article | 22 January 2012

  Optimizing the specificity of nucleic acid hybridization

  High-fidelity pairing of nucleic acid polymers is important in the development of sensors and for the application of DNA nanotechnology. Here, a set of hybridization probes is described that discriminates single-base changes with high specificity. The probes function robustly across many different temperatures, salinities and nucleic acid concentrations.

  • David Yu Zhang
  • , Sherry Xi Chen
  •  & Peng Yin