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| Open AccessRandom-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution
Random-access wide-field mesoscopy enables the imaging of in vivo biodynamics in mice over an area of 160 mm2 and at a subcellular spatial resolution of about 2 μm.
- Ruheng Shi
- , Xinyue Chen
- & Lingjie Kong
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News & Views |
Single protein imaging with holography
A non-common-path interferometric scheme enables holographic detection of single proteins of mass 90 kDa and estimation of single-protein polarizability.
- Chia-Lung Hsieh
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Article
| Open AccessSingle-protein optical holography
Holographic microscopy with independent control of the signal and reference fields enables the holographic imaging of a single protein with mass below 100 kDa and estimation of their polarizability.
- Jan Christoph Thiele
- , Emanuel Pfitzner
- & Philipp Kukura
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Article
| Open AccessAttosecond electron microscopy by free-electron homodyne detection
Free-electron homodyne detection allows measuring phase-resolved optical responses in electron microscopy, demonstrated in the imaging of plasmonic fields with few-nanometre spatial and sub-cycle temporal resolutions.
- John H. Gaida
- , Hugo Lourenço-Martins
- & Claus Ropers
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Article
| Open AccessPlasmonic photoconductive terahertz focal-plane array with pixel super-resolution
A terahertz focal-plane array based on a two-dimensional array of plasmonic photoconductive nanoantennas offers high-quality imaging in the terahertz region.
- Xurong Li
- , Deniz Mengu
- & Mona Jarrahi
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Review Article |
Label-free biomedical optical imaging
This Review covers a comparison between various label-free biomedical imaging techniques, their advantages over label-based methods and relevant applications.
- Natan T. Shaked
- , Stephen A. Boppart
- & Jürgen Popp
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Article
| Open AccessSingle multimode fibre for in vivo light-field-encoded endoscopic imaging
Spatial-frequency tracking adaptive beacon light-field encoded endoscopy enables imaging through a single multimode fibre under bending and twisting. In vivo imaging with subcellular resolution is demonstrated in mice models.
- Zhong Wen
- , Zhenyu Dong
- & Qing Yang
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Article |
Bond-selective fluorescence imaging with single-molecule sensitivity
Two-photon excitation with mid- and near-infrared pulses encodes bond selectivity in fluorescence imaging. Single-molecule imaging and spectroscopy is demonstrated on individual fluorophores as well as various labelled biological targets.
- Haomin Wang
- , Dongkwan Lee
- & Lu Wei
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Article |
A double-tapered fibre array for pixel-dense gamma-ray imaging
Researchers engineer double-tapered optical-fibre arrays and use perovskite nanocrystal substrates for X-ray imaging with a three orders of magnitude output gain and spatial resolution of 22 lp mm−1. Arrayed gamma-ray imaging is also demonstrated using a nanocrystal scintillator film.
- Luying Yi
- , Bo Hou
- & Xiaogang Liu
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Letter
| Open AccessDual-comb hyperspectral digital holography
Dual-comb digital holography based on an interferometer composed of two frequency combs of slightly different repetition frequencies and a lensless camera sensor allows highly frequency-multiplexed holography with high temporal coherence.
- Edoardo Vicentini
- , Zhenhai Wang
- & Nathalie Picqué
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Review Article |
Nanoscale terahertz scanning probe microscopy
Recent progress in terahertz scanning probe microscopy is reviewed with an emphasis on techniques that access length scales below 100 nm relevant to material science. An outlook on the future of nanoscale terahertz scanning probe microscopy is also provided.
- T. L. Cocker
- , V. Jelic
- & F. A. Hegmann
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Letter |
Scattering invariant modes of light in complex media
The concept of scattering invariant modes is introduced to produce the same transmitted field profiles through a multiple scattering sample and a reference medium. Their correlations with the ballistic light can be used to improve imaging inside scattering materials.
- Pritam Pai
- , Jeroen Bosch
- & Allard P. Mosk
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Review Article |
Variable optical elements for fast focus control
Recent improvements of the operation speed of variable optical elements are reviewed with an emphasis on components with microsecond focus-varying response time.
- SeungYeon Kang
- , Martí Duocastella
- & Craig B. Arnold
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Article |
Perovskite-filled membranes for flexible and large-area direct-conversion X-ray detector arrays
Perovskite-filled-membranes enable flexible, sensitive and large-area X-ray detectors. The structures are made by infiltrating perovskite solution into porous polymer membranes.
- Jingjing Zhao
- , Liang Zhao
- & Jinsong Huang
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Article |
Broadband coherent diffractive imaging
Coherent diffractive imaging using broadband illumination is demonstrated at visible and X-ray wavelengths. The method is based on a numerical monochromatization of the broadband diffraction pattern by the regularized inversion of a matrix.
- Julius Huijts
- , Sara Fernandez
- & Hamed Merdji
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News & Views |
Super-resolution microscopy on a photonic chip
Using a photonic chip to generate the patterns of light needed for structured illumination microscopy could reduce the cost and complexity of super-resolution imaging.
- Sara Abrahamsson
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Letter |
Luminescent surfaces with tailored angular emission for compact dark-field imaging devices
A luminescent photonic substrate with a controlled angular emission profile is introduced and its ability to generate high-contrast dark-field images of micrometre-sized living organisms is demonstrated using standard optical microscopy equipment.
- Cécile A. C. Chazot
- , Sara Nagelberg
- & Mathias Kolle
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News & Views |
Overcoming the colour barrier
High-efficiency, time-domain, near-infrared fluorophores provide multiplexed colour channels for distinct deep bioimaging.
- Shoujun Zhu
- & Xiaoyuan Chen
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Letter |
High-sensitivity imaging of time-domain near-infrared light transducer
By time-shifting short-pulse excitation photon energy into prolonged luminescent emission in the time domain, both the number of light signal transducers in sub-15 nm nanoparticles and the near-infrared-in to near-infrared-out conversion efficiency can be maximized, advancing in vivo optical bioimaging.
- Yuyang Gu
- , Zhiyong Guo
- & Fuyou Li
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Article |
Computational time-of-flight diffuse optical tomography
By combining a single-photon time-of-flight camera with computational processing of the spatial and full temporal photon distribution data, an object embedded inside a strongly diffusive medium can be imaged over more than 80 transport mean free paths in a contactless manner on the timescale of the order of 1 s.
- Ashley Lyons
- , Francesco Tonolini
- & Daniele Faccio
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Letter |
Super-resolution retinal imaging using optically reassigned scanning laser ophthalmoscopy
The implementation of optically reassigned scanning laser ophthalmoscopy enables in vivo photon reassignment super-resolution imaging and high-resolution imaging of living human retinal cone photoreceptor cells without adaptive optics or chemical dilation of the eye.
- Theodore B. DuBose
- , Francesco LaRocca
- & Joseph A. Izatt
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News & Views |
Folding photography in the time domain
Exploiting an optical cavity that folds space in time in a conventional lens design provides a novel route for time-resolved imaging and depth sensing.
- Sylvain Gigan
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Article |
Photography optics in the time dimension
By folding large spaces in time using an off-resonant Fabry–Pérot cavity in camera sensors, new capabilities such as ultrafast multi-zoom imaging and ultrafast multispectral imaging, of use for time-resolved imaging and depth-sensing optics, are found.
- Barmak Heshmat
- , Matthew Tancik
- & Ramesh Raskar
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News & Views |
The super-resolution debate
In the quest for nanoscopy with super-resolution, consensus from the imaging community is that super-resolution is not always needed and that scientists should choose an imaging technique based on their specific application.
- Rachel Won
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Letter |
Far-field nanoscopy on a semiconductor quantum dot via a rapid-adiabatic-passage-based switch
A spatial resolution of 30 nm (=λ/31) exceeding the diffraction limit is achieved by super-resolution fluorescence microscopy. The nanoscopic imaging scheme can be applied to coherent quantum-mechanical systems such as quantum dots, as well as colour centres.
- Timo Kaldewey
- , Andreas V. Kuhlmann
- & Richard J. Warburton
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Review Article |
X-ray ptychography
This Review covers key advancements in X-ray ptychographic microscopy and tomography over the past ten years. Potential applications in the life and materials sciences, the latest concepts and future developments are also discussed.
- Franz Pfeiffer
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Article |
Ultrasensitive plano-concave optical microresonators for ultrasound sensing
An optical-based scheme for ultrasound sensing provides very high sensitivity with excellent broadband acoustic frequency response and wide directivity.
- James A. Guggenheim
- , Jing Li
- & Paul C. Beard
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Article |
Depolarization signatures map gold nanorods within biological tissue
An optical technique makes it possible to determine the distribution of gold nanoparticles in tissue.
- Norman Lippok
- , Martin Villiger
- & Brett E. Bouma
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Article |
Broadband image sensor array based on graphene–CMOS integration
Graphene–quantum dots on CMOS sensor offers broadband imaging.
- Stijn Goossens
- , Gabriele Navickaite
- & Frank Koppens
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Article |
High-temporal-resolution electron microscopy for imaging ultrafast electron dynamics
Ultrafast electron microscopy with an order-of-magnitude enhancement in the typical temporal resolution is demonstrated, permitting the imaging of ultrafast electron dynamics that last a few tens of femtoseconds.
- M. Th. Hassan
- , J. S. Baskin
- & A. H. Zewail
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Article |
High-resolution adaptive imaging of a single atom
The position of a single Yb atomic ion is determined with a minimum uncertainty of 1.7 nm for 0.2 s integration time — the highest position sensitivity reported to date for an isolated atom.
- J. D. Wong-Campos
- , K. G. Johnson
- & C. Monroe
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Article |
Two-photon direct laser writing of ultracompact multi-lens objectives
Femtosecond two-photon direct laser writing is used to create 100-µm-scale high-performance multi-lens objectives.
- Timo Gissibl
- , Simon Thiele
- & Harald Giessen
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Letter |
Real-space mapping of tailored sheet and edge plasmons in graphene nanoresonators
Researchers demonstrate graphene plasmon edge modes at infrared wavelengths. Such modes may offer additional electromagnetic field confinement compared with conventional sheet modes.
- A. Y. Nikitin
- , P. Alonso-González
- & R. Hillenbrand
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Commentary |
Standardizing the resolution claims for coherent microscopy
The definition and reporting of spatial resolution for coherent imaging methods varies widely in the imaging community. We advocate the use of a standard spoke-pattern imaging target and the mandatory inclusion of information about underlying a priori assumptions.
- Roarke Horstmeyer
- , Rainer Heintzmann
- & Changhuei Yang
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Letter |
Femtosecond and nanometre visualization of structural dynamics in superheated nanoparticles
Single Xe clusters are superheated using an intense optical laser pulse and the structural evolution is imaged with a single X-ray pulse. Ultrafast surface softening on the nanometre scale is resolved within 100 fs at the vacuum/sample interface.
- Tais Gorkhover
- , Sebastian Schorb
- & Christoph Bostedt
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Progress Article |
Non-fluorescent schemes for single-molecule detection, imaging and spectroscopy
Various optical schemes that can detect single molecules without the need of fluorescence are discussed.
- Jaime Ortega Arroyo
- & Philipp Kukura
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Article |
Seeing through chaos in multimode fibres
The prediction of light propagation up to hundreds of millimetres within straight or even deformed segments of multimode fibres is demonstrated. The concept is applied in an endoscope and exceptional resolution and footprint are obtained.
- Martin Plöschner
- , Tomáš Tyc
- & Tomáš Čižmár
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Article |
Imaging deep within a scattering medium using collective accumulation of single-scattered waves
Using the collective accumulation of single-scattered waves, scientists manage to image deep within a scattering medium, achieving an imaging depth 11.5 times the scattering mean free path and a near-diffraction-limit resolution of 1.5 µm.
- Sungsam Kang
- , Seungwon Jeong
- & Wonshik Choi
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Article |
Photoacoustically guided wavefront shaping for enhanced optical focusing in scattering media
Nonlinear photoacoustic signals are used to ‘guide’ laser light to a tight focal spot in scattering media.
- Puxiang Lai
- , Lidai Wang
- & Lihong V. Wang
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News & Views |
Device-level photonic testing
Non-invasive, multispectral characterization of integrated photonic circuits paves the way towards optical methodologies ready for industrial applications.
- Matteo Burresi
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Article |
High-throughput imaging of heterogeneous cell organelles with an X-ray laser
70,000 diffraction patterns captured over twelve minutes at the Linac Coherent Light Source yield reconstructions of the smallest single biological objects imaged with an X-ray laser.
- Max F. Hantke
- , Dirk Hasse
- & Inger Andersson
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Interview |
Femtophotography
A burst-mode camera developed in Japan called STAMP with a femtosecond frame rate could become a powerful tool for studying ultrafast dynamics. Nature Photonics asked Keiichi Nakagawa about the technique.
- Noriaki Horiuchi
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Letter |
Sequentially timed all-optical mapping photography (STAMP)
A single-shot burst camera has been developed that can generate motion pictures without performing repetitive measurements. It has a frame rate of 4.4 trillion frames per second and a high pixel resolution of 450 × 450 pixels, making it a powerful tool for observing difficult-to-reproduce or non-repetitive events in real time.
- K. Nakagawa
- , A. Iwasaki
- & I. Sakuma
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Article |
Shape-induced force fields in optical trapping
Through shaping of colloidal particles, optical traps with prescribed force–displacement profiles are generated and are used to design a microscopic constant-force spring capable of delivering a constant piconewton-scale restoring force for displacements of several micrometres. Potential future applications include the imaging of sensitive biological membranes.
- D. B. Phillips
- , M. J. Padgett
- & S. H. Simpson
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Letter |
An ultrafast terahertz scanning tunnelling microscope
An ultrafast terahertz (THz) scanning tunnelling microscope (STM) with subpicosecond time resolution and nanometre spatial resolution has been developed. THz pulses are coupled to the metal tip of a commercial STM and THz-pulse-induced tunnelling is observed in the STM. The THz-STM can directly image ultrafast carrier capture by a single InAs nanodot.
- Tyler L. Cocker
- , Vedran Jelic
- & Frank A. Hegmann
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