Gene targeting articles within Scientific Reports

Featured

• Article
  31 January 2024 | Open Access

  Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues

  • Thomas L. Maurissen
  • , Masahide Kawatou
  •  & Knut Woltjen

• Article
  10 November 2023 | Open Access

  Transcriptomic analysis reveals that RasGEF1b deletion alters basal and LPS-induced expression of genes involved in chemotaxis and cytokine responses in macrophages

  • Heliana B. Fernandes
  • , Isadora Mafra de Oliveira
  •  & Aristóbolo M. Silva

• Article
  31 October 2023 | Open Access

  CRISPR/Cas9-mediated knock-in of BRCA1/2 mutations restores response to olaparib in pancreatic cancer cell lines

  • Andréa Witz
  • , Julie Dardare
  •  & Alexandre Harlé

• Article
  19 July 2023 | Open Access

  Long-read sequence analysis of MMEJ-mediated CRISPR genome editing reveals complex on-target vector insertions that may escape standard PCR-based quality control

  • Yuki Higashitani
  •  & Kyoji Horie

• Article
  19 May 2023 | Open Access

  Generation of CRISPR-Cas9-mediated knockin mutant models in mice and MEFs for studies of polymorphism in clock genes

  • Kwangjun Lee
  •  & Choogon Lee

• Article
  31 January 2023 | Open Access

  Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC

  • Sameer Singh
  • , Anoosha Banerjee
  •  & Sebastian Klinge

• Article
  13 December 2022 | Open Access

  Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice

  • Manabu Ozawa
  • , Jumpei Taguchi
  •  & Masahito Ikawa

• Article
  24 November 2022 | Open Access

  Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells

  • Mariangela Pappadà
  • , Ottavia Bonuccelli
  •  & Peggy Marconi

• Article
  16 July 2022 | Open Access

  CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

  • D. J. Jedrzejczyk
  • , L. D. Poulsen
  •  & R. T. Gill

• Article
  02 July 2022 | Open Access

  CRISPR/Cas9-based genome-wide screening of Dictyostelium

  • Takanori Ogasawara
  • , Jun Watanabe
  •  & Tetsuya Muramoto

• Article
  19 November 2021 | Open Access

  Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking

  • Md. Lutfur Rahman
  • , Toshinori Hyodo
  •  & Hiroyuki Konishi

• Article
  12 November 2021 | Open Access

  First report of mitochondrial COI in foraminifera and implications for DNA barcoding

  • Jan-Niklas Macher
  • , Jeremy G. Wideman
  •  & Willem Renema

• Article
  14 September 2021 | Open Access

  Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii

  • Yasuhiko Matsumoto
  • , Tae Nagamachi
  •  & Takashi Sugita

• Article
  13 September 2021 | Open Access

  Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase θ/DNA-PK

  • Daisuke Arai
  •  & Yoichi Nakao

• Article
  23 July 2021 | Open Access

  Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish

  • Cheng-Yu Li
  • , Joshua R. Steighner
  •  & Scott A. Juntti

• Article
  13 July 2021 | Open Access

  HtrA4 is up-regulated during trophoblast syncytialization and BeWo cells fail to syncytialize without HtrA4

  • Mary Mansilla
  • , Yao Wang
  •  & Guiying Nie

• Article
  06 July 2021 | Open Access

  Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity

  • Valerie Sapp
  • , Aitor Aguirre
  •  & Mohit Jain

• Article
  21 June 2021 | Open Access

  A genome-wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP-1 cells

  • Benjamin Lindner
  • , Eva Martin
  •  & Michael Schuler

• Article
  17 May 2021 | Open Access

  CRISPR-mediated mutations in the ABC transporter gene ABCA2 confer pink bollworm resistance to Bt toxin Cry2Ab

  • Jeffrey A. Fabrick
  • , Dannialle M. LeRoy
  •  & Bruce E. Tabashnik

• Article
  05 May 2021 | Open Access

  Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

  • Bjorn Thor Adalsteinsson
  • , Thordis Kristjansdottir
  •  & Gudmundur Oli Hreggvidsson

• Article
  05 April 2021 | Open Access

  Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux

  • Gülsüm Kayman Kürekçi
  • , Ecem Kural Mangit
  •  & Pervin R. Dincer

• Article
  13 January 2021 | Open Access

  CRISPR-based transcriptional activation tool for silent genes in filamentous fungi

  • László Mózsik
  • , Mirthe Hoekzema
  •  & Arnold J. M. Driessen

• Article
  03 December 2020 | Open Access

  Improving CRISPR/Cas9 mutagenesis efficiency by delaying the early development of zebrafish embryos

  • M. Terzioglu
  • , A. Saralahti
  •  & J.-O. Andressoo

• Article
  24 August 2020 | Open Access

  Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence

  • Riki Ishibashi
  • , Kota Abe
  •  & Fumiko Toyoshima

• Article
  18 August 2020 | Open Access

  Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing

  • Yuji Yokouchi
  • , Shinichi Suzuki
  •  & Takumi Era

• Article
  28 July 2020 | Open Access

  Physiological impact and comparison of mutant screening methods in piwil2 KO founder Nile tilapia produced by CRISPR/Cas9 system

  • Ye Hwa Jin
  • , Baoshan Liao
  •  & Andrew Davie

• Article
  23 June 2020 | Open Access

  Amyloid precursor protein-b facilitates cell adhesion during early development in zebrafish

  • Rakesh Kumar Banote
  • , Jasmine Chebli
  •  & Henrik Zetterberg

• Article
  06 February 2020 | Open Access

  TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae

  • Jin Han
  • , Zhihui Xia
  •  & Wenxue Zhai

• Article
  23 January 2020 | Open Access

  Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9

  • Takayuki Sakurai
  • , Akiko Kamiyoshi
  •  & Takayuki Shindo

• Article
  27 December 2019 | Open Access

  Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method

  • Satoshi Hara
  • , Miho Terao
  •  & Shuji Takada

• Article
  23 October 2019 | Open Access

  Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry

  • Qifeng Lin
  • , Larry W. L. Low
  •  & Tom J. Carney

• Article
  10 October 2019 | Open Access

  Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

  • Harvie P. Portugaliza
  • , Oriol Llorà-Batlle
  •  & Alfred Cortés

• Article
  03 September 2019 | Open Access

  Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing

  • Wakako Kumita
  • , Kenya Sato
  •  & Erika Sasaki

• Article
  17 May 2019 | Open Access

  CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes

  • Alex K. Datsomor
  • , Nikola Zic
  •  & Per Winge

• Article
  27 December 2018 | Open Access

  The mineralocorticoid receptor is essential for stress axis regulation in zebrafish larvae

  • Erin Faught
  •  & Mathilakath M. Vijayan

• Article
  07 November 2018 | Open Access

  Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish

  • Ahmed Elaswad
  • , Karim Khalil
  •  & Rex Dunham

• Article
  13 August 2018 | Open Access

  i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

  • Shuji Takabayashi
  • , Takuya Aoshima
  •  & Masahiro Sato

• Article
  31 May 2018 | Open Access

  CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium

  • Ryoya Sekine
  • , Takefumi Kawata
  •  & Tetsuya Muramoto

• Article
  01 February 2018 | Open Access

  ‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

  • Q. Guo
  • , G. Mintier
  •  & John N. Feder

• Article
  23 January 2018 | Open Access

  Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L)

  • Alexandra Hübner
  • , Bjoern Petersen
  •  & Walter Fuchs

• Article
  18 January 2018 | Open Access

  A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis

  • Hongmin Zhang
  • , Yuexin Zhou
  •  & Wensheng Wei

• Article
  10 January 2018 | Open Access

  Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells

  • Kentaro Ishida
  • , Huaigeng Xu
  •  & Akitsu Hotta

• Article
  08 January 2018 | Open Access

  Combined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism

  • Liat Peretz
  • , Elazar Besser
  •  & Oded Behar

• Article
  01 December 2017 | Open Access

  Molecular Identification of ten species of stored-product psocids through microarray method based on ITS2 rDNA

  • Li-Jun Liu
  • , Ao-Han Pang
  •  & Zhi-Hong Li

• Article
  23 August 2017 | Open Access

  Development of a versatile and conventional technique for gene disruption in filamentous fungi based on CRISPR-Cas9 technology

  • Yan-Mei Zheng
  • , Fu-Long Lin
  •  & Dan Hu

• Article
  15 August 2017 | Open Access

  Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

  • Xuemei Zhang
  • , Wenrong Li
  •  & Mingjun Liu

• Article
  31 July 2017 | Open Access

  Modeling Dominant and Recessive Forms of Retinitis Pigmentosa by Editing Three Rhodopsin-Encoding Genes in Xenopus Laevis Using Crispr/Cas9

  • Joanna M. Feehan
  • , Colette N. Chiu
  •  & Orson L. Moritz

• Article
  24 May 2017 | Open Access

  Deletion of Runx2 in Articular Chondrocytes Decelerates the Progression of DMM-Induced Osteoarthritis in Adult Mice

  • Lifan Liao
  • , Shanxing Zhang
  •  & Di Chen

• Article
  08 May 2017 | Open Access

  CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3′ UTR induce graying in rabbit

  • Yuning Song
  • , Yuxin Xu
  •  & Zhanjun Li

• Article
  19 April 2017 | Open Access

  Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9

  • Ming Li
  • , Lauren Yun Cook Au
  •  & Omar S. Akbari