Gene targeting

Definition

Gene targeting is the process of altering a specific sequence or gene at its location in a genome. Potential modifications include deletion, insertion or replacement of endogenous sequence with alternative sequences. Targeting can be achieved by homologous recombination in some organisms (notably, mice), or with site-directed genome editing nucleases.

Latest Research and Reviews

  • Research | | open

    Chromatin looping plays an important role in gene regulation and the ability to manipulate loops would aid in understanding how this occurs. Here the authors present CLOuD9, a system that uses dimerized Cas9 complexes to selectively and reversibly establish chromatin loops.

    • Stefanie L. Morgan
    • , Natasha C. Mariano
    • , Abel Bermudez
    • , Nicole L. Arruda
    • , Fangting Wu
    • , Yunhai Luo
    • , Gautam Shankar
    • , Lin Jia
    • , Huiling Chen
    • , Ji-Fan Hu
    • , Andrew R. Hoffman
    • , Chiao-Chain Huang
    • , Sharon J. Pitteri
    •  & Kevin C. Wang
  • Research | | open

    Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sites in vitro and in vivo.

    • Yong Lei
    • , Xiaotian Zhang
    • , Jianzhong Su
    • , Mira Jeong
    • , Michael C. Gundry
    • , Yung-Hsin Huang
    • , Yubin Zhou
    • , Wei Li
    •  & Margaret A. Goodell
  • Research | | open

    Site-specific recombination and CRISPR-Cas9 have been used to generate genetically engineered mouse models of cancer. Here the authors compare sarcomas generated using both systems and see similar genetic and cellular phenotypes, suggesting CRISPR-Cas9 can be used to rapidly generate sarcoma models.

    • Jianguo Huang
    • , Mark Chen
    • , Melodi Javid Whitley
    • , Hsuan-Cheng Kuo
    • , Eric S. Xu
    • , Andrea Walens
    • , Yvonne M. Mowery
    • , David Van Mater
    • , William C. Eward
    • , Diana M. Cardona
    • , Lixia Luo
    • , Yan Ma
    • , Omar M. Lopez
    • , Christopher E. Nelson
    • , Jacqueline N. Robinson-Hamm
    • , Anupama Reddy
    • , Sandeep S. Dave
    • , Charles A. Gersbach
    • , Rebecca D. Dodd
    •  & David G. Kirsch
  • Research | | open

    Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and protein delivery of base editors to improve their DNA specificity and enable specific base editing in live animals.

    • Holly A. Rees
    • , Alexis C. Komor
    • , Wei-Hsi Yeh
    • , Joana Caetano-Lopes
    • , Matthew Warman
    • , Albert S. B. Edge
    •  & David R. Liu
  • Research | | open

    CRISPR/Cas9 gene editing has been used to generate mutations in several mouse genes. Here, the authors show that targeting events using single guide RNAs cause large deletions at 17 sites in the mouse genome, suggesting that careful genotyping is needed and sequential targeting may avoid such deletions.

    • Ha Youn Shin
    • , Chaochen Wang
    • , Hye Kyung Lee
    • , Kyung Hyun Yoo
    • , Xianke Zeng
    • , Tyler Kuhns
    • , Chul Min Yang
    • , Teresa Mohr
    • , Chengyu Liu
    •  & Lothar Hennighausen

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