Cytological techniques articles within Polymer Journal

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• Original Article | 27 February 2013

  A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis

  A novel peptide-array format system with a photo-cleavage linker for cellular toxicity analysis was constructed. In this system, a peptide was immobilized on a commercially available plate bottom via the photo-cleavable linker. UV light irradiation on the desired wells releases the peptide from the plate bottom, and then the cytotoxic behavior of the peptide can be monitored.

  • Takashi Kakiyama
  • , Kenji Usui
  •  & Hisakazu Mihara

• Original Article | 12 December 2012

  Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimensional cell culture

  Two and three times repeats of the self-assembling peptide RADA16 (RADARADARADARADA), namely RADA16x2 and RADA16x3, were genetically produced. The peptides were expressed as fusion proteins while retaining the activity of the fusion partner protein. The expressed peptides exhibited both fibril formation and cell adhesive activity. Moreover, the hydrogels formed by the peptides via addition of a medium provided a three-dimensional environment for cell proliferation.

  • Masayasu Mie
  • , Mayu Oomuro
  •  & Eiry Kobatake

• Original Article | 20 June 2012

  Poly(N-isopropylacrylamide)-modified silica beads with hyperbranched polysiloxysilane for three-dimensional cell cultivation

  The objective of this study is to establish an immobilization method of PNIPAM on silica beads surface-modified by HBPS, which could be applied to cell cultivation. The PNIPAM-modified silica beads with enough loading of N-isopropylacrylamide (cleaved PNIPAM M_n=14 800 and 23 700) shows controlled cell adhesion and detachment due to lower critical solution temperature behavior of PNIPAM.

  • Bo Ra Park
  • , Yuta Nabae
  •  & Masa-aki Kakimoto