Research Highlight |
Featured
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Editorial |
Setting standards for stem cells
New traceability and reporting standards aim to improve transparency in stem cell research.
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Article |
Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing
Femtosecond laser microdissection enables transcriptomic analyses of single neurons based on their phenotype.
- Peisen Zhao
- , Sudip Mondal
- & Adela Ben-Yakar
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Research Highlight |
Next-generation expansion microscopy
A new twist on expansion microscopy called Magnify uses a mechanically sturdy gel to simultaneously anchor and expand diverse biological samples for super-resolution imaging.
- Rita Strack
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Research Highlight |
Modeling multi-organ systems on a chip
To recapitulate some of the complexities of the human physiology, engineered tissues can be linked by vascular flow in a multi-organ chip.
- Nina Vogt
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Correspondence |
Best practices for reporting throughput in biomedical research
- Maik Herbig
- , Akihiro Isozaki
- & Keisuke Goda
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Editorial |
Catching up with multiplexed tissue imaging
Highly multiplexed tissue imaging continues to show its power for biomedical discovery. In this issue, we publish tools and guidance for implementing this class of methods and reporting subsequent results.
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Research Highlight |
Recapitulating miRNA biogenesis in cells
MapToCleave enables the large-scale study of miRNA precursor processing in living cells.
- Lei Tang
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Article |
Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D
Anterograde transneuronal tracing can be achieved with engineered variants of the yellow fever vaccine YFV-17D. Both monosynaptic and polysynaptic tracing are demonstrated in different circuits of the mouse brain.
- Elizabeth Li
- , Jun Guo
- & Wei Xu
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Article |
SEAM is a spatial single nuclear metabolomics method for dissecting tissue microenvironment
SEAM is a platform for the analysis of high-resolution secondary ion mass spectrometry imaging that allows spatially resolved nuclear metabolomic profiling at the single-cell level.
- Zhiyuan Yuan
- , Qiming Zhou
- & Michael Q. Zhang
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Research Highlight |
Bespoke antibodies fast tracked
A yeast cell culture approach mimics the benefits of somatic hypermutation to accelerate the discovery of desirable antibodies.
- Katarzyna Marcinkiewicz
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Research Highlight |
Organoid systems to recapitulate cervical cancer
Human cervical organoids mimic dynamics of cancer and sexually transmitted infections.
- Madhura Mukhopadhyay
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Research Highlight |
Human tonsils in a dish
Human tonsil organoids recapitulate key features of the antigen-specific B cell response.
- Madhura Mukhopadhyay
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Article |
3D mapping and accelerated super-resolution imaging of the human genome using in situ sequencing
OligoFISSEQ combines Oligopaints with fluorescence in situ sequencing to enable the 3D mapping of many regions across the genome in human cells to interrogate genome organization at improved genomic resolution. OligoFISSEQ is compatible with immunochemistry and OligoSTORM for super-resolution imaging.
- Huy Q. Nguyen
- , Shyamtanu Chattoraj
- & C.-ting Wu
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Brief Communication |
Elasticizing tissues for reversible shape transformation and accelerated molecular labeling
The ELAST technology renders large tissue samples elastic through embedding in hydrogel and facilitates sample handling and rapid molecular labeling.
- Taeyun Ku
- , Webster Guan
- & Kwanghun Chung
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Research Highlight |
A mouse blastocyst-like structure from scratch
Researchers show that mouse stem and adult cells can develop into blastocyst-like structures.
- Vesna Todorovic
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Article |
Engineering of human brain organoids with a functional vascular-like system
Expression of ETV2 in human cortical organoids induces the formation of vascular-like networks, which reduces cell death within organoids and increases their functional maturation.
- Bilal Cakir
- , Yangfei Xiang
- & In-Hyun Park
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Article |
An active texture-based digital atlas enables automated mapping of structures and markers across brains
An active atlas for automatic alignment of brains to a reference atlas is presented. The method uses the fine-scale pattern of tissue. The atlas is refined by each new brain and can inform on the structural variability between different brains.
- Yuncong Chen
- , Lauren E. McElvain
- & David Kleinfeld
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Resource |
A mutant-cell library for systematic analysis of heparan sulfate structure–function relationships
A library of mutant mouse lung endothelial cells expressing a comprehensive repertoire of heparin sulfate structure modifications enables studies of the structure–function relationships of this complex polysaccharide.
- Hong Qiu
- , Songshan Shi
- & Lianchun Wang
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Brief Communication |
Rapid and efficient induction of functional astrocytes from human pluripotent stem cells
A simple transcription-factor-based protocol generates functional astrocytes rapidly and efficiently from human pluripotent stem cells.
- Isaac Canals
- , Aurélie Ginisty
- & Henrik Ahlenius
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Resource |
The GAGOme: a cell-based library of displayed glycosaminoglycans
A CHO cell library displaying a near-complete repertoire of glycosaminoglycan (GAG) modifications provides a resource for cell-based binding assays, recombinant proteoglycan expression, and assembly of GAG glycan microarrays.
- Yen-Hsi Chen
- , Yoshiki Narimatsu
- & Zhang Yang
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Research Highlight |
A home for brain organoids
Inside a mouse brain, human cerebral organoids can show their potential.
- Tal Nawy
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Methods in Brief |
From somatic cells to naive pluripotent cells
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Brief Communication |
Real-time fluorescence and deformability cytometry
RT-FDC combines the specificity of fluorescent probes with functional readout of a cell's mechanical properties. The resulting correlation of fluorescence-based cell identity with mechanical measurements in real time allows the development of label-free, mechanical cell sorting.
- Philipp Rosendahl
- , Katarzyna Plak
- & Jochen Guck
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Tools in Brief |
Biobanking breast cancers
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Article |
BRCA-deficient mouse mammary tumor organoids to study cancer-drug resistance
Mouse tumor organoids are characterized as a model to study tumor biology and drug resistance.
- Alexandra A Duarte
- , Ewa Gogola
- & Sven Rottenberg
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Tools in Brief |
A yTRAP for protein aggregates
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Tools in Brief |
Stem cells tip toward totipotency
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Article |
Isolation and 3D expansion of multipotent Sox9+ mouse lung progenitors
This paper describes methods for the 3D culture of mouse lung progenitor cells that can differentiate in vitro and in vivo along all epithelial lineages.
- Massimo Nichane
- , Asif Javed
- & Bing Lim
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Research Highlights |
Antibodies trigger reprogramming
Antibodies that initiate signaling cascades and thereby replace reprogramming factors promise an efficient and safe way to produce pluripotent stem cells.
- Nicole Rusk
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Article |
Long-term expansion of alveolar stem cells derived from human iPS cells in organoids
This paper reports the efficient generation of human alveolar cells from induced pluripotent stem cells and their expansion in 3D culture.
- Yuki Yamamoto
- , Shimpei Gotoh
- & Michiaki Mishima
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Methods in Brief |
Quantitative clearing-enabled tissue analysis
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Brief Communication |
Testing for differential abundance in mass cytometry data
A statistical approach makes it possible to detect differentially abundant cell populations across biological conditions from high-dimensional mass cytometry data without relying on cell clustering.
- Aaron T L Lun
- , Arianne C Richard
- & John C Marioni
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Article |
Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
This paper describes a fully defined, nonxenogeneic in vitro niche for the differentiation of haematopoietic stem and progenitor cells to progenitor T cells in mouse and human.
- Shreya Shukla
- , Matthew A Langley
- & Peter W Zandstra
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Brief Communication |
DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
DeActs are genetically encoded tools that perturb the actin cytoskeleton. In contrast to drugs such as latrunculin, they can be targeted to specific cell types, which is demonstrated in the developing mouse brain and in Caenorhabditis elegans.
- Martin Harterink
- , Marta Esteves da Silva
- & J Bradley Zuchero
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Article |
Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids
This paper describes an in vitro method to generate human T cells from hematopoietic stem and progenitor cells (HSPCs). It should be useful for both basic and applied studies using T cells.
- Christopher S Seet
- , Chongbin He
- & Amélie Montel-Hagen
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Method to Watch |
Organoid culture
Ex vivo organoid culture could revolutionize biology, but variability must be understood.
- Natalie de Souza
Greater diversity for lineage tracing
Putting on the squeeze
Imaging organoids: a bright future ahead