Abstract
TWIST1 is a highly conserved basic helix-loop-helix transcription factor that promotes epithelial–mesenchymal transition (EMT). Its misregulation has been observed in various types of tumors. Using the MCF-10A-series of cell lines that recapitulate the early stages of breast cancer formation and EMT, we found TWIST1 to be upregulated during EMT and downregulated early in carcinogenesis. The TWIST1 3′UTR contains putative regulatory elements, including miRNA target sites and two cytoplasmic polyadenylation elements (CPE). We found that miR-580, CPEB1, and CPEB2 act as negative regulators of TWIST1 expression in a sequence-specific and additive/cooperative manner.
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Acknowledgements
We thank Valentina Evdokimova for providing us with the MCF-10ANeoT-MSCV and MCF-10ANeoT-YB-1 cell lines, Linda Louise Pritchard for editorial and scientific advice, and Leslie-Ann Largitte for technical assistance. This work was supported in part by grant n° LSHG-CT-2006-037900 from the European Commission Sixth Framework Programme to AHB and by grant n° DP08036 from The Danish Cancer Society to EMF.
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Nairismägi, ML., Vislovukh, A., Meng, Q. et al. Translational control of TWIST1 expression in MCF-10A cell lines recapitulating breast cancer progression. Oncogene 31, 4960–4966 (2012). https://doi.org/10.1038/onc.2011.650
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DOI: https://doi.org/10.1038/onc.2011.650
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