Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme

Latest Research

  • Article |

    Cryo-EM structures of two late-stage assembly intermediates of the human mitoribosomal large subunit reveal the timing of rRNA folding and protein incorporation during the final steps of ribosomal maturation and identify two new assembly factors.

    • Alan Brown
    • , Sorbhi Rathore
    • , Dari Kimanius
    • , Shintaro Aibara
    • , Xiao-chen Bai
    • , Joanna Rorbach
    • , Alexey Amunts
    •  & V Ramakrishnan
  • Article |

    Crystal structures of unprocessed and mature crRNA-bound LbaCas13a shed light upon catalytic residues involved in crRNA maturation and mechanisms blocking Cas13a nuclease activity before target-RNA binding.

    • Gavin J Knott
    • , Alexandra East-Seletsky
    • , Joshua C Cofsky
    • , James M Holton
    • , Emeric Charles
    • , Mitchell R O'Connell
    •  & Jennifer A Doudna
  • Review |

    In this Review, Peña, Hurt and Panse discuss our current knowledge on the eukaryotic ribosome assembly, a complex process that takes place across different cellular compartments and involves over 200 assembly factors.

    • Cohue Peña
    • , Ed Hurt
    •  & Vikram Govind Panse
  • Resource |

    A comprehensive proteomics screen for ‘reader’ proteins that recognize m6A-modified RNA reveals that the modification both promotes and prevents the binding of factors that control mRNA homeostasis in mammalian cells.

    • Raghu R Edupuganti
    • , Simon Geiger
    • , Rik G H Lindeboom
    • , Hailing Shi
    • , Phillip J Hsu
    • , Zhike Lu
    • , Shuang-Yin Wang
    • , Marijke P A Baltissen
    • , Pascal W T C Jansen
    • , Martin Rossa
    • , Markus Müller
    • , Hendrik G Stunnenberg
    • , Chuan He
    • , Thomas Carell
    •  & Michiel Vermeulen

News & Comment

  • News and Views |

    The monoallelic expression of many imprinted genes in mammals depends on DNA methylation marks that originate from the germ cells. Recent studies in mice and fruit flies evoke a novel, transient mode of genomic imprinting in which oocyte-acquired histone H3 Lys27 trimethylation (H3K27me3) marks are transmitted to the zygote and modulate the allele specificity and timing of gene expression in the early embryo.

    • Rakesh Pathak
    •  & Robert Feil
  • News and Views |

    The robustness of the circadian clock deteriorates with aging. Two new studies show that aging reprograms the circadian transcriptome in a cell-type-dependent manner and that such rewiring can be reversed by caloric restriction.

    • Rika Ohkubo
    •  & Danica Chen
  • News and Views |

    Group II chaperonins facilitate protein folding by undergoing ATP-driven conformational changes. A recent study reveals a tunable allosteric network in group II chaperonins that includes a residue at the intersubunit interface, which is important for assembly and allosteric coordination. The authors also propose that lower cooperativity allows group II chaperonins to achieve optimal substrate folding over a broad range of ATP concentrations.

    • Mingliang Jin
    •  & Yao Cong
  • News and Views |

    Meiotic progression is controlled by cytoplasmic polyadenylation and translational activation of masked, maternal mRNAs. RNA-binding-protein interactions with adjacent cis elements cause local conformational changes to the mRNAs that determine the extent and timing of their activation.

    • Paul Lasko
  • News and Views |

    An unusual pairing of homologous X chromosomes occurs during X inactivation. A new study in mouse embryonic stem cells shows that telomeres and the telomeric RNA PAR-TERRA are responsible for additional pairwise interactions that guide Xic–Xic pairing.

    • Ivan Krivega
    •  & Ann Dean

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