Supplementary information

From the following article:

Cycling hypoxia and free radicals regulate angiogenesis and radiotherapy response

Mark W. Dewhirst, Yiting Cao & Benjamin Moeller

Nature Reviews Cancer 8, 425-437 (June 2008)

doi:10.1038/nrc2397

Supplementary information S1 (movie)

Phosphorescence lifetime imaging was performed to image vascular oxygen concentration in 2x3 mm regions of skin fold window chamber tumors of Fischer rats. The windows contained either the 9L glioma (movie 1) or a fibrosarcoma (movie 2). Oxygen measurements were made every 2.5 min for 60-90 min. The movies depict changes in pO2 that are >5 mmHg from the immediately preceding image. Red indicates an increase, Blue indicates a decrease and yellow indicates no changes >5mmHg. Note that in both examples there are a significant number of pixels showing this behavior, with the fibrosarcoma showing more of a tendency to exhibit contiguous regions of similar behaviour.

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Supplementary information S2 (movie)

Phosphorescence lifetime imaging was performed to image vascular oxygen concentration in 2x3 mm regions of skin fold window chamber tumors of Fischer rats. The windows contained either the 9L glioma (movie 1) or a fibrosarcoma (movie 2). Oxygen measurements were made every 2.5 min for 60-90 min. The movies depict changes in pO2 that are >5 mmHg from the immediately preceding image. Red indicates an increase, Blue indicates a decrease and yellow indicates no changes >5mmHg. Note that in both examples there are a significant number of pixels showing this behavior, with the fibrosarcoma showing more of a tendency to exhibit contiguous regions of similar behaviour.

Download Movie (12.4 MB)

Supplementary information S3 (figure)

Expression of HIF-1 reporter protein (Green fluorescence protein; GFP) during angiogenesis initiation and acceleration.

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