Volume 14 Issue 7, July 2019

This protocol describes the design, application and computational analysis of high-dimensional fluorescent antibody panels for flow cytometry. Up to 28 colors can be characterized to study complex cellular populations such as the immune system.

X¹³CMS is used to annotate signals in untargeted, liquid chromatography/mass spectrometry, metabolomic datasets after samples are labeled with an isotopic tracer. The results provide insight into novel biochemical transformations and metabolic flux.

This protocol describes how to derive and maintain mouse haploid embryonic stem cells (hESCs) from female gametes. Additional procedures that can be carried out with cell lines obtained from the mouse cell resource Haplobank are also described.

This protocol describes how to fabricate and use a wireless nanopore electrode (WNE). The WNE has metal at the tip of a nanopipette, which acts as an electroactive sensing interface, providing electrochemical measurements of single entities.

This protocol describes the steps in GRID-seq, a procedure used to capture RNA in the proximity of chromatin in situ, thus enabling systematic detection of RNA–chromatin interactions in mammalian genomes.

This protocol describes how to establish stable transgenic lines of the freshwater polyp Hydra by embryo microinjection.

This protocol describes a solvent-assisted method to prepare supported lipid bilayers (SLBs) with minimal sample preparation. The approach is quick, easy to implement, and compatible with a wide range of lipid compositions and material supports.

Here, the authors describe the use of synthetic DNA spike-ins, called sequins, as internal quantitative and qualitative controls throughout the sequencing workflow. They detail their application in whole-genome and targeted human genome sequencing.

This protocol describes how to use an open-source toolbox, DeepLabCut, to train a deep neural network to precisely track user-defined features with limited training data. This allows noninvasive behavioral tracking of movement.

This protocol describes how to synthesize high-surface-area dendritic fibrous nanosilica (DFNS) with tunable sizes, textural properties and fiber density at different scales. It also includes the application of DFNSs in catalysis (Suzuki coupling) and CO₂ capture.

A protocol for the assembly and use of the optoPlate-96, a platform for high-throughput three-color optogenetics experiments in microwell plates. With the provided design files, users can assemble the optoPlate-96 from 3D-printed and laser-cut components.

This protocol outlines the chemical synthesis of proteins via a recently discovered reaction called diselenide–selenoester ligation (DSL) in a rapid, additive-free manner. After ligation, products can be chemoselectively deselenized to produce native peptide and protein products.