Volume 12 Issue 1, January 2017

Different concentrations of activin A and BMP4 are used to polarize stem cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm.

This protocol describes how to produce retinotopic maps of mouse visual cortex using intrinsic signal optical imaging and a segmentation algorithm.

Zilionis et al. describe a protocol for the inDrops platform, which is a high-throughput droplet microfluidics system that allows for single-cell barcoding of over 15,000 cells in 1 h.

This protocol describes the diastereoselective approach for the synthesis of complex, acyclic molecular architectures possessing two stereogenic centers in a 1,4 relationship based on a zirconium-promenade reaction using the Negishi reagent.

This protocol uses a doxycycline-inducible Cas9 transgene carried on a piggyBac transposon for robust, highly efficient and scarless genome editing of human iPSCs, enabling a parental line to be engineered to harbor many separate mutations.

This protocol describes surgical techniques for long-term intrathecal drug administration to rodent CNS tissue and cerebrospinal fluid collection for monitoring of drug concentration and distribution.

Winz et al. describe a protocol for NAD captureSeq, a method to capture and identify NAD-capped RNA sequences.

Correlative light and electron microscopy (CLEM) promises new insight into cellular processes by combining spatiotemporal information with high-resolution structural information. This protocol describes cryo-CLEM of virus-infected mammalian cells.

This protocol describes how to generate mouse 3D immune organoids consisting of germinal-center-like B cells in a gelatin matrix.

Correia-Melo et al. describe a protocol to generate and maintain mitochondria-depleted mammalian cell lines. These cells can be used to investigate the role of mitochondria in various cellular processes such as cell death and senescence.

This protocol describes how to differentiate human pluripotent stem cells into nephron progenitor cells with subsequent generation of 2D and 3D kidney organoids.