Volume 3 Issue 4, April 2008

Acclimatized transgenic cotton plantlets, being grown individually for analysis. Transgenic plants are cultured in vitro from embryonic axes that have been transformed by the bombardment of apical meristems with microparticle-coated DNA. Image is from the protocol by Rech et al. Cover design by Jessica Iannuzzi.

Protocol

The prion organotypic slice culture assay—POSCA

Jeppe Falsig
Adriano Aguzzi
Protocol 13 Mar 2008
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MicroRNA expression profiling using microarrays

Chang-Gong Liu
George Adrian Calin
Carlo M Croce
Protocol 13 Mar 2008
Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization

Markus Hafner
Elena Vianini
Michael Famulok
Protocol 13 Mar 2008
Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis

Y John Shyu
Susan M Hiatt
Chang-Deng Hu
Protocol 13 Mar 2008
MRI of postmortem specimens of endangered species for comparative brain anatomy

Jeremy R Corfield
J Martin Wild
M Fabiana Kubke
Protocol 13 Mar 2008
Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy

Stephen L Rogers
Gregory C Rogers
Protocol 20 Mar 2008
Protocol for the induction of arthritis in C57BL/6 mice

Julia J Inglis
Egle Šimelyte
Richard O Williams
Protocol 20 Mar 2008
Colocalization of fluorescent markers in confocal microscope images of plant cells

Andrew P French
Steven Mills
Tony P Pridmore
Protocol 20 Mar 2008
Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals

Jung-Joon Min
Vu H Nguyen
Hyon E Choy
Protocol 20 Mar 2008
Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae

Jennifer E Fox
Matthew E Burow
Charles A Miller III
Protocol 20 Mar 2008
Solid-phase synthesis of pseudo-complementary peptide nucleic acids

Makoto Komiyama
Yuichiro Aiba
Jun Sumaoka
Protocol 27 Mar 2008
Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity

Makoto Komiyama
Yuichiro Aiba
Jun Sumaoka
Protocol 27 Mar 2008
Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation

Gaspar Banfalvi
Protocol 27 Mar 2008
Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression

Celeste M Nelson
Jamie L Inman
Mina J Bissell
Protocol 27 Mar 2008
NMR: prediction of molecular alignment from structure using the PALES software

Markus Zweckstetter
Protocol 27 Mar 2008
Facile synthesis of mono-6-amino-6-deoxy-α-, β-, γ-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery

Weihua Tang
Siu-Choon Ng
Protocol 03 Apr 2008
Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans

Arnab Mukhopadhyay
Bart Deplancke
Heidi A Tissenbaum
Protocol 03 Apr 2008
A protocol describing the principles of cis-regulatory analysis in the sea urchin

Joel Smith
Protocol 03 Apr 2008
Cardiac tissue engineering using perfusion bioreactor systems

Milica Radisic
Anna Marsano
Gordana Vunjak-Novakovic
Protocol 03 Apr 2008
A protocol for expression of foreign genes in chloroplasts

Dheeraj Verma
Nalapalli P Samson
Henry Daniell
Protocol 03 Apr 2008

Volume 3 Issue 4, April 2008

Protocol

The prion organotypic slice culture assay—POSCA

MicroRNA expression profiling using microarrays

Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization

Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis

MRI of postmortem specimens of endangered species for comparative brain anatomy

Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy

Protocol for the induction of arthritis in C57BL/6 mice

Colocalization of fluorescent markers in confocal microscope images of plant cells

Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals

Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae

Solid-phase synthesis of pseudo-complementary peptide nucleic acids

Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity

Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation

Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression

NMR: prediction of molecular alignment from structure using the PALES software

Facile synthesis of mono-6-amino-6-deoxy-α-, β-, γ-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery

Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans

A protocol describing the principles of cis-regulatory analysis in the sea urchin

Cardiac tissue engineering using perfusion bioreactor systems

A protocol for expression of foreign genes in chloroplasts

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