Latest Protocols
Volume 3 No 4
- Archive
In order to view all protocols, including both Nature Protocols and Protocols Network protocols, please browse by category.
To facilitate archiving and citing, Nature Protocols are allocated to publication months and assigned unique page numbers.
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Nature protocols
The prion organotypic slice culture assay—POSCA - pp555 - 562
Jeppe Falsig & Adriano Aguzzi
Published online: 13 March 2008 | doi:10.1038/nprot.2008.13
Abstract - The prion organotypic slice culture assay[mdash]POSCA | Full Text - The prion organotypic slice culture assay—POSCA | PDF (380 KB) - The prion organotypic slice culture assay—POSCA
MicroRNA expression profiling using microarrays - pp563 - 578
Chang-Gong Liu, George Adrian Calin, Stefano Volinia & Carlo M Croce
Published online: 13 March 2008 | doi:10.1038/nprot.2008.14
Abstract - MicroRNA expression profiling using microarrays | Full Text - MicroRNA expression profiling using microarrays | PDF (1,079 KB) - MicroRNA expression profiling using microarrays
Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization - pp579 - 587
Markus Hafner, Elena Vianini, Barbara Albertoni, Laura Marchetti, Imke Grüne, Christian Gloeckner & Michael Famulok
Published online: 13 March 2008 | doi:10.1038/nprot.2008.15
Abstract - Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization | Full Text - Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization | PDF (422 KB) - Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization
Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis - pp588 - 596
Y John Shyu, Susan M Hiatt, Holli M Duren, Ronald E Ellis, Tom K Kerppola & Chang-Deng Hu
Published online: 13 March 2008 | doi:10.1038/nprot.2008.16
Abstract - Visualization of protein interactions in living : Caenorhabditis elegans: using bimolecular fluorescence complementation analysis | Full Text - Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis | PDF (261 KB) - Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis
MRI of postmortem specimens of endangered species for comparative brain anatomy - pp597 - 605
Jeremy R Corfield, J Martin Wild, Brett R Cowan, Stuart Parsons & M Fabiana Kubke
Published online: 13 March 2008 | doi:10.1038/nprot.2008.17
Abstract - MRI of postmortem specimens of endangered species for comparative brain anatomy | Full Text - MRI of postmortem specimens of endangered species for comparative brain anatomy | PDF (652 KB) - MRI of postmortem specimens of endangered species for comparative brain anatomy
Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy - pp606 - 611
Stephen L Rogers & Gregory C Rogers
Published online: 20 March 2008 | doi:10.1038/nprot.2008.18
Abstract - Culture of : Drosophila: S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy | Full Text - Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy | PDF (216 KB) - Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy
Protocol for the induction of arthritis in C57BL/6 mice - pp612 - 618
Julia J Inglis,
Egle 
imelyte,
Fiona E McCann,
Gabriel Criado
&
Richard O Williams
Published online: 20 March 2008 | doi:10.1038/nprot.2008.19
Abstract - Protocol for the induction of arthritis in C57BL/6 mice | Full Text - Protocol for the induction of arthritis in C57BL/6 mice | PDF (296 KB) - Protocol for the induction of arthritis in C57BL/6 mice
Colocalization of fluorescent markers in confocal microscope images of plant cells - pp619 - 628
Andrew P French, Steven Mills, Ranjan Swarup, Malcolm J Bennett & Tony P Pridmore
Published online: 20 March 2008 | doi:10.1038/nprot.2008.31
Abstract - Colocalization of fluorescent markers in confocal microscope images of plant cells | Full Text - Colocalization of fluorescent markers in confocal microscope images of plant cells | PDF (464 KB) - Colocalization of fluorescent markers in confocal microscope images of plant cells
Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals - pp629 - 636
Jung-Joon Min, Vu H Nguyen, Hyun-Ju Kim, Yeongjin Hong & Hyon E Choy
Published online: 20 March 2008 | doi:10.1038/nprot.2008.32
Abstract - Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals | Full Text - Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals | PDF (417 KB) - Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals
Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae - pp637 - 645
Jennifer E Fox, Matthew E Burow, John A McLachlan & Charles A Miller, III
Published online: 20 March 2008 | doi:10.1038/nprot.2008.33
Abstract - Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast : Saccharomyces cerevisiae | Full Text - Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae | PDF (241 KB) - Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae
Solid-phase synthesis of pseudo-complementary peptide nucleic acids - pp646 - 654
Makoto Komiyama, Yuichiro Aiba, Takumi Ishizuka & Jun Sumaoka
Published online: 27 March 2008 | doi:10.1038/nprot.2008.6
Abstract - Solid-phase synthesis of pseudo-complementary peptide nucleic acids | Full Text - Solid-phase synthesis of pseudo-complementary peptide nucleic acids | PDF (261 KB) - Solid-phase synthesis of pseudo-complementary peptide nucleic acids
Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity - pp655 - 662
Makoto Komiyama, Yuichiro Aiba, Yoji Yamamoto & Jun Sumaoka
Published online: 27 March 2008 | doi:10.1038/nprot.2008.7
Abstract - Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity | Full Text - Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity | PDF (306 KB) - Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity
Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation - pp663 - 673
Gaspar Banfalvi
Published online: 27 March 2008 | doi:10.1038/nprot.2008.34
Abstract - Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation | Full Text - Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation | PDF (351 KB) - Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation
Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression - pp674 - 678
Celeste M Nelson, Jamie L Inman & Mina J Bissell
Published online: 27 March 2008 | doi:10.1038/nprot.2008.35
Abstract - Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression | Full Text - Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression | PDF (352 KB) - Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression
NMR: prediction of molecular alignment from structure using the PALES software - pp679 - 690
Markus Zweckstetter
Published online: 27 March 2008 | doi:10.1038/nprot.2008.36
Abstract - NMR: prediction of molecular alignment from structure using the PALES software | Full Text - NMR: prediction of molecular alignment from structure using the PALES software | PDF (332 KB) - NMR: prediction of molecular alignment from structure using the PALES software | Supplementary information
Facile synthesis of mono-6-amino-6-deoxy-
-, 
-, 
-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery - pp691 - 697
Weihua Tang & Siu-Choon Ng
Published online: 03 April 2008 | doi:10.1038/nprot.2008.37
Abstract - Facile synthesis of mono-6-amino-6-deoxy-[alpha]-, [beta]-, [gamma]-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery | Full Text - Facile synthesis of mono-6-amino-6-deoxy-
-, 
-, 
-cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery | PDF (371 KB) - Facile synthesis of mono-6-amino-6-deoxy--, -, -cyclodextrin hydrochlorides for molecular recognition, chiral separation and drug delivery
Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans - pp698 - 709
Arnab Mukhopadhyay, Bart Deplancke, Albertha J M Walhout & Heidi A Tissenbaum
Published online: 03 April 2008 | doi:10.1038/nprot.2008.38
Abstract - Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in : Caenorhabditis elegans | Full Text - Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans | PDF (316 KB) - Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans
A protocol describing the principles of cis-regulatory analysis in the sea urchin - pp710 - 718
Joel Smith
Published online: 03 April 2008 | doi:10.1038/nprot.2008.39
Abstract - A protocol describing the principles of : cis: -regulatory analysis in the sea urchin | Full Text - A protocol describing the principles of cis-regulatory analysis in the sea urchin | PDF (324 KB) - A protocol describing the principles of cis-regulatory analysis in the sea urchin
Cardiac tissue engineering using perfusion bioreactor systems - pp719 - 738
Milica Radisic, Anna Marsano, Robert Maidhof, Yadong Wang & Gordana Vunjak-Novakovic
Published online: 03 April 2008 | doi:10.1038/nprot.2008.40
Abstract - Cardiac tissue engineering using perfusion bioreactor systems | Full Text - Cardiac tissue engineering using perfusion bioreactor systems | PDF (768 KB) - Cardiac tissue engineering using perfusion bioreactor systems
A protocol for expression of foreign genes in chloroplasts - pp739 - 758
Dheeraj Verma, Nalapalli P Samson, Vijay Koya & Henry Daniell
Published online: 03 April 2008 | doi:10.1038/nprot.2007.522
Abstract - A protocol for expression of foreign genes in chloroplasts | Full Text - A protocol for expression of foreign genes in chloroplasts | PDF (612 KB) - A protocol for expression of foreign genes in chloroplasts
