Latest Protocols
Volume 3 No 1
- Archive
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Nature protocols
Use of Raman spectroscopy as a tool for in situ monitoring of microwave-promoted reactions - pp1 - 7
Nicholas E Leadbeater & Jason R Schmink
Published online: 20 December 2007 | doi:10.1038/nprot.2007.453
Abstract - | Full Text - Use of Raman spectroscopy as a tool for in situ monitoring of microwave-promoted reactions | PDF (805 KB) - Use of Raman spectroscopy as a tool for in situ monitoring of microwave-promoted reactions
Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine - pp8 - 21
Jacek Zielonka, Jeannette Vasquez-Vivar & Balaraman Kalyanaraman
Published online: 20 December 2007 | doi:10.1038/nprot.2007.473
Abstract - | Full Text - Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine | PDF (470 KB) - Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine
Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry - pp22 - 33
Montse Morell, Alba Espargaro, Francesc Xavier Aviles & Salvador Ventura
Published online: 20 December 2007 | doi:10.1038/nprot.2007.496
Abstract - | Full Text - Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry | PDF (493 KB) - Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry
Purification and culture of nerve growth factor receptor (p75)-expressing basal forebrain cholinergic neurons - pp34 - 40
Aletta C Schnitzler, Ignacio Lopez-Coviella & Jan Krzysztof Blusztajn
Published online: 20 December 2007 | doi:10.1038/nprot.2007.477
Abstract - | Full Text - Purification and culture of nerve growth factor receptor (p75)-expressing basal forebrain cholinergic neurons | PDF (425 KB) - Purification and culture of nerve growth factor receptor (p75)-expressing basal forebrain cholinergic neurons | Supplementary information
Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets - pp41 - 50
Luke E Berchowitz & Gregory P Copenhaver
Published online: 20 December 2007 | doi:10.1038/nprot.2007.491
Abstract - | Full Text - Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets | PDF (434 KB) - Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets | Supplementary information
Southwestern blotting in investigating transcriptional regulation - pp51 - 58
Francis K Y Siu, Leo T O Lee & Billy K C Chow
Published online: 20 December 2007 | doi:10.1038/nprot.2007.492
Abstract - | Full Text - Southwestern blotting in investigating transcriptional regulation | PDF (205 KB) - Southwestern blotting in investigating transcriptional regulation
High-resolution in situ hybridization to whole-mount zebrafish embryos - pp59 - 69
Christine Thisse & Bernard Thisse
Published online: 20 December 2007 | doi:10.1038/nprot.2007.514
Abstract - | Full Text - High-resolution in situ hybridization to whole-mount zebrafish embryos | PDF (524 KB) - High-resolution in situ hybridization to whole-mount zebrafish embryos
Methods for detection, isolation and culture of mouse and human invariant NKT cells - pp70 - 78
Hiroshi Watarai, Ryusuke Nakagawa, Miyuki Omori-Miyake, Nyambayar Dashtsoodol & Masaru Taniguchi
Published online: 20 December 2007 | doi:10.1038/nprot.2007.515
Abstract - | Full Text - Methods for detection, isolation and culture of mouse and human invariant NKT cells | PDF (383 KB) - Methods for detection, isolation and culture of mouse and human invariant NKT cells
Gene knockdown by ecdysone-based inducible RNAi in stable mammalian cell lines - pp79 - 88
Danny Rangasamy, David J Tremethick & Ian K Greaves
Published online: 10 January 2008 | doi:10.1038/nprot.2007.456
Abstract - | Full Text - Gene knockdown by ecdysone-based inducible RNAi in stable mammalian cell lines | PDF (347 KB) - Gene knockdown by ecdysone-based inducible RNAi in stable mammalian cell lines
Preparation of peptide-conjugated quantum dots for tumor vasculature-targeted imaging - pp89 - 96
Weibo Cai & Xiaoyuan Chen
Published online: 10 January 2008 | doi:10.1038/nprot.2007.478
Abstract - | Full Text - Preparation of peptide-conjugated quantum dots for tumor vasculature-targeted imaging | PDF (353 KB) - Preparation of peptide-conjugated quantum dots for tumor vasculature-targeted imaging
Regioselective one-pot protection of glucose - pp97 - 113
Cheng-Chung Wang, Suvarn S Kulkarni, Jinq-Chyi Lee, Shun-Yuan Luo & Shang-Cheng Hung
Published online: 10 January 2008 | doi:10.1038/nprot.2007.493
Abstract - | Full Text - Regioselective one-pot protection of glucose | PDF (393 KB) - Regioselective one-pot protection of glucose
2-Nitroglycals: versatile intermediates for efficient and highly stereoselective base-catalyzed glycoside bond formations - pp114 - 121
B Gopal Reddy & Richard R Schmidt
Published online: 10 January 2008 | doi:10.1038/nprot.2007.495
Abstract - | Full Text - 2-Nitroglycals: versatile intermediates for efficient and highly stereoselective base-catalyzed glycoside bond formations | PDF (224 KB) - 2-Nitroglycals: versatile intermediates for efficient and highly stereoselective base-catalyzed glycoside bond formations
A mouse model of intracerebral hemorrhage using autologous blood infusion - pp122 - 128
Michal A Rynkowski, Grace H Kim, Ricardo J Komotar, Marc L Otten, Andrew F Ducruet, Brad E Zacharia, Christopher P Kellner, David K Hahn, Maxwell B Merkow, Matthew C Garrett, Robert M Starke, Byung-Moon Cho, Sergei A Sosunov & E Sander Connolly
Published online: 10 January 2008 | doi:10.1038/nprot.2007.513
Abstract - | Full Text - A mouse model of intracerebral hemorrhage using autologous blood infusion | PDF (297 KB) - A mouse model of intracerebral hemorrhage using autologous blood infusion
The regular and light–dark Suok tests of anxiety and sensorimotor integration: utility for behavioral characterization in laboratory rodents - pp129 - 136
Allan V Kalueff, Tiina Keisala, Anna Minasyan, Senthil R Kumar, Justin L LaPorte, Dennis L Murphy & Pentti Tuohimaa
Published online: 10 January 2008 | doi:10.1038/nprot.2007.516
Abstract - | Full Text - The regular and light–dark Suok tests of anxiety and sensorimotor integration: utility for behavioral characterization in laboratory rodents | PDF (289 KB) - The regular and light–dark Suok tests of anxiety and sensorimotor integration: utility for behavioral characterization in laboratory rodents | Supplementary information
Synthesis of enantiomerically enriched (R)-5-tert-butylazepan-2-one using a hydroxyalkyl azide mediated ring-expansion reaction - pp137 - 143
Timothy P Ribelin & Jeffrey Aubé
Published online: 10 January 2008 | doi:10.1038/nprot.2007.518
Abstract - | Full Text - Synthesis of enantiomerically enriched (R)-5-tert-butylazepan-2-one using a hydroxyalkyl azide mediated ring-expansion reaction | PDF (292 KB) - Synthesis of enantiomerically enriched (R)-5-tert-butylazepan-2-one using a hydroxyalkyl azide mediated ring-expansion reaction
Immunogold quantification of amino acids and proteins in complex subcellular compartments - pp144 - 152
Linda H Bergersen, Jon Storm-Mathisen & Vidar Gundersen
Published online: 10 January 2008 | doi:10.1038/nprot.2007.525
Abstract - | Full Text - Immunogold quantification of amino acids and proteins in complex subcellular compartments | PDF (412 KB) - Immunogold quantification of amino acids and proteins in complex subcellular compartments