Protocol abstract
Nature Protocols 2, - 1 - 4 (2007)
Published online: 31 January 2007 | doi:10.1038/nprot.2007.17
Subject Categories: Cell and tissue culture | Model organisms | Microbiology and virology
Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method
R Daniel Gietz1 & Robert H Schiestl2
Abstract
Here we describe a protocol for the production of frozen competent yeast cells that can be transformed with high efficiency using the lithium acetate/single-stranded carrier DNA/PEG method. This protocol allows the production of highly competent yeast cells that can be frozen and used at a later date and is especially useful for laboratories using one or two strains repeatedly. The production of yeast cells for freezing takes only approximately 30 min, once the yeast culture has grown up. Transformation with frozen competent yeast cells will take approximately 30 min, depending on the heat shock used.
- Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
- Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, California 90095, USA.
Correspondence to: Robert H Schiestl2 e-mail: rschiestl@mednet.ucla.edu
nature-products
A-Z product listing
- 1.5 ml polypropylene microcentrifuge tubes(Fisher Scientific Ltd)
- 100 tube styrofoam rack with lid(Sarstedt Inc)
- 12 ml 17
100 mm sterile polypropylene culture tube(Fisher Scientific Ltd) - 2 liter baffled Erlenmyer flask(Fisher Scientific Ltd)
- 250 ml baffled Erlenmyer flask(Fisher Scientific Ltd)
- 50 ml polypropylene sterile disposable centrifuge tubes(Fisher Scientific Ltd)
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.